公开(公告)号：US20180373837A1

公开(公告)日：2018-12-27

申请号：US16117976

申请日：2018-08-30

Applicant: Genia Technologies, Inc.

Inventor： Santiago Fernandez-Gomez ,  Hui Tian ,  J. William Maney ,  Jayalakshmi Rajaraman

IPC: G06F19/22 ,  G01N33/487

Abstract: A system includes a plurality of nanopore cells. Data corresponding to nanopore states of the plurality of nanopore cells is received. The data is analyzed to determine a compressed output size of the data given at least one compression technique. It is determined whether the compressed output size exceeds a data budget. In the event it is determined that the compressed output size exceeds the data budget, the data is modified. The modified data is outputted.

公开(公告)号：US20180267013A1

公开(公告)日：2018-09-20

申请号：US15983426

申请日：2018-05-18

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  David J. Fullagar

IPC: G01N33/487

Abstract: A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.

公开(公告)号：US20180245147A1

公开(公告)日：2018-08-30

申请号：US15443964

申请日：2017-02-27

Applicant: Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Preethi Sarvabhowman ,  Dhruti Vasudev Dalal

IPC: C12Q1/68 ,  C12N9/12 ,  G01N27/447

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  C12Q2521/101 ,  C12Q2565/631

Abstract: The present of disclosure provides variant Pol6 polymerase polypeptide, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result n increased processivity relative to the parental Pol6 polypeptides from which they are derived.

公开(公告)号：US20180100827A1

公开(公告)日：2018-04-12

申请号：US15819994

申请日：2017-11-21

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/327 ,  G01N33/487

CPC classification number: G01N27/3278 ,  C12Q1/6874 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system for detecting electrical properties of a molecular complex is disclosed. The system includes an electrode electrically coupled to a molecular complex that outputs an electrical signal affected by an electrical property of the molecular complex, wherein the effect of the electrical property of the molecular complex on the electrical signal is characterized by an expected bandwidth. The system further includes an integrating amplifier circuit configured to receive the electrical signal from the electrode. The integrating amplifier circuit is further configured to selectively amplify and integrate a portion of the electrical signal over time within a predetermined bandwidth, wherein the predetermined bandwidth is selected at least in part based on the expected bandwidth.

公开(公告)号：US20180017543A1

公开(公告)日：2018-01-18

申请号：US15699542

申请日：2017-09-08

Applicant: Genia Technologies, Inc.

Inventor： Robert A. Yuan

IPC: G01N33/487 ,  C12Q1/68 ,  G01N27/447

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791

Abstract: A nanopore based sequencing system is disclosed. The system includes a plurality of nanopore sensors, each nanopore sensor having a top portion for receiving a fluid. The system further includes an inlet delivering the fluid into the nanopore based sequencing system and an outlet delivering the fluid out of the nanopore based sequencing system. The system includes a fluid chamber that comprises one or more fluid flow channels above top portions of the nanopore sensors; wherein the fluid chamber includes at least one divider that limits the width of the one or more fluid flow channels. In some embodiments, the at least one divider limits the width of the one or more fluid flow channel based on whether the surface tension and adhesive forces between the fluid and the fluid flow channel surfaces are sufficient to prevent the fluid from collapsing within the fluid flow channel.

公开(公告)号：US20170254797A1

公开(公告)日：2017-09-07

申请号：US15462483

申请日：2017-03-17

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  David J. Fullagar

IPC: G01N33/487

CPC classification number: G01N33/48721

Abstract: A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.

公开(公告)号：US20170088588A1

公开(公告)日：2017-03-30

申请号：US14924861

申请日：2015-10-28

Applicant: Genia Technologies, Inc.

Inventor： Michael DORWART ,  Daniel KORENBLUM

IPC: C07K14/31 ,  C12Q1/68

CPC classification number: C07K14/31 ,  C12Q1/6869 ,  C12Q2565/631

Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The α-hemolysin variants have a decreased time to thread.

公开(公告)号：US20170059546A1

公开(公告)日：2017-03-02

申请号：US14841127

申请日：2015-08-31

Applicant: Genia Technologies, Inc.

Inventor： John Foster ,  Morgan Mager

IPC: G01N33/487 ,  G01N27/447 ,  H01L29/40

Abstract: A nanopore cell includes a conductive layer and a working electrode disposed above the conductive layer and at the bottom of a well into which an electrolyte may be contained, such that at least a portion of a top base surface area of the working electrode is exposed to the electrolyte. The nanopore cell further includes a first insulating wall disposed above the working electrode and surrounding a lower section of a well, and a second insulating wall disposed above the first insulating wall and surrounding an upper section of the well, forming an overhang above the lower section of the well. The upper section of the well includes an opening that a membrane may span across, and wherein a base surface area of the opening is smaller than the at least a portion of the top base surface area of the working electrode that is exposed to the electrolyte.

Abstract translation: 纳米孔单元包括导电层和工作电极，其设置在导电层上方和可以容纳电解质的阱的底部，使得工作电极的顶部基面表面区域的至少一部分暴露于 电解液。 纳米孔单元还包括设置在工作电极上方并围绕阱的下部的第一绝缘壁和设置在第一绝缘壁上方并围绕阱的上部的第二绝缘壁，在第二绝缘壁的下部 的井。 阱的上部包括膜可跨越的开口，并且其中开口的基面面积小于暴露于电解质的工作电极的顶部基面表面区域的至少一部分。

公开(公告)号：US20160333327A1

公开(公告)日：2016-11-17

申请号：US15151364

申请日：2016-05-10

Applicant: Genia Technologies, Inc.

Inventor： Aruna AYER ,  Cleoma ARNOLD ,  Charles Wayan SCHWAB ,  Eileen THAI ,  Ilya LEDERMAN ,  Colin Alexander MCGAW ,  Tyler O'Brien SHULTZ

IPC: C12N9/12

Abstract: Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, 1295, Y342, T343, I357, S360, L361, I363, S365, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.

Abstract translation: 本文描述的是具有至少一个选自H223，N224，Y225，H227,1295，Y342，T343，I357，S360，L361，I363，S365，S366，Y367，P368，D417，E475，Y476， F478，K518，H527，T529，M531，N535，G539，P542，N545，Q546，A547，L549，I550，N552，G553，F558，A596，G603，A610，V615，Y622，C623，D624，I628，Y629， R632，N635，M641，A643，I644，T647，I648，T651，I652，K655，W656，D657，V658，H660，F662，L690及其组合。

公开(公告)号：US20160274082A1

公开(公告)日：2016-09-22

申请号：US14664484

申请日：2015-03-20

Applicant: Genia Technologies, Inc.

Inventor： Robert A. Yuan

IPC: G01N33/487 ,  G01N27/447 ,  C12Q1/68

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791

Abstract: A nanopore based sequencing system is disclosed. The system includes a plurality of nanopore sensors, each nanopore sensor having a top portion for receiving a fluid. The system further includes an inlet delivering the fluid into the nanopore based sequencing system and an outlet delivering the fluid out of the nanopore based sequencing system. The system includes a fluid chamber that comprises one or more fluid flow channels above top portions of the nanopore sensors; wherein the fluid chamber includes at least one divider that limits the width of the one or more fluid flow channels. In some embodiments, the at least one divider limits the width of the one or more fluid flow channel based on whether the surface tension and adhesive forces between the fluid and the fluid flow channel surfaces are sufficient to prevent the fluid from collapsing within the fluid flow channel.

Abstract translation: 公开了一种基于纳米孔的测序系统。 该系统包括多个纳米孔传感器，每个纳米孔传感器具有用于接收流体的顶部部分。 该系统还包括将流体输送到基于纳米孔的测序系统中的入口和将流体输出基于纳米孔的测序系统的出口。 该系统包括流体室，该流体室包括在纳米孔传感器顶部上方的一个或多个流体流动通道; 其中所述流体室包括限制所述一个或多个流体流动通道的宽度的至少一个分隔件。 在一些实施例中，所述至少一个分离器基于流体和流体流动通道表面之间的表面张力和粘合力是否足以防止流体在流体流动内塌陷来限制一个或多个流体流动通道的宽度 渠道。