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    Polypeptides having glucoamylase activity and polynucleotides encoding same
    61.
    发明授权
    Polypeptides having glucoamylase activity and polynucleotides encoding same 失效
    具有葡糖淀粉酶活性的多肽和编码该多肽的多核苷酸

    公开(公告)号：US08697412B2

    公开(公告)日：2014-04-15

    申请号：US13687389

    申请日：2012-11-28

    Applicant: Novozymes A/S ,  Novozymes North America, Inc.

    Inventor： Sara Landvik ,  Keiichi Ayabe ,  Guillermo Coward-Kelly

    IPC: C12N9/34 ,  C07K14/00 ,  C12N1/00 ,  C12P1/00

    CPC classification number: C12N9/26 ,  C12N9/2428 ,  C12P7/06 ,  C12P7/14 ,  C12P19/14 ,  Y02E50/17

    Abstract: The present invention relates to isolated polypeptides having glucoamylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.

    Abstract translation: 本发明涉及具有葡糖淀粉酶活性的分离多肽，催化结构域，碳水化合物结合结构域和编码多肽，催化结构域或碳水化合物结合结构域的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽，催化结构域或碳水化合物结合结构域的方法。

    Fermentation processes and compositions
    64.
    发明申请
    Fermentation processes and compositions 审中-公开
    发酵过程和组成

    公开(公告)号：US20040253696A1

    公开(公告)日：2004-12-16

    申请号：US10459315

    申请日：2003-06-10

    Applicant: Novozymes North America, Inc.

    Inventor： Varvara Grichko

    IPC: C12P007/06

    CPC classification number: C12P7/06 ,  C12P7/08 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

    Abstract: The present invention provides improved fermentation processes, including for use in an ethanol production process. The improved fermentation processes include applying at least one fatty acid oxidizing enzyme (such as a lipoxygenase) in a fermentation process. The improved fermentation process may also involve the addition of various additional enzymes and growth stimulators for the fermenting microorganisms, including vitamins and mineral.

    Abstract translation: 本发明提供改进的发酵方法，包括用于乙醇生产方法。 改进的发酵方法包括在发酵过程中应用至少一种脂肪酸氧化酶（例如脂氧合酶）。 改进的发酵方法还可以包括为发酵微生物（包括维生素和矿物质）添加各种另外的酶和生长刺激剂。

    Use of cloud-point extraction
    66.
    发明申请
    Use of cloud-point extraction 失效
    使用云点提取

    公开(公告)号：US20040147001A1

    公开(公告)日：2004-07-29

    申请号：US10734431

    申请日：2003-12-12

    Applicant: Novozymes A/S ,  Novozymes North America, Inc.

    Inventor： Prashant Iyer ,  Kishore Rane ,  Kevin S. Wenger ,  Fahd Azzabi

    IPC: C12N009/02 ,  C12N009/20 ,  C12N009/24 ,  C12N009/28 ,  C12N009/42 ,  C12N009/64

    CPC classification number: C07K1/36 ,  C07K1/145 ,  C12N9/00 ,  Y10S435/816

    Abstract: A method of extracting a polypeptide of interest from a fermentation broth comprising: i) adjusting the pH close to pI of the polypeptide of interest; ii) adding a non-ionic surfactant with a hydrophile-lipophile balance (HLB) of 12 or lower; iii) cooling the mixture for solubilization and incubating at above cloud point for extraction; iv) phase separating at below cloud point to obtain liquid-liquid-solid fractions; and v) recovering the surfactant-rich top phase containing the polypeptide of interest.

    Abstract translation: 一种从发酵液中提取目的多肽的方法，包括：i）调节pH值接近目的多肽的pI; ii）加入亲水亲油平衡（HLB）为12或更低的非离子表面活性剂; iii）冷却该混合物以溶解并在上述浊点温育以提取; iv）在低于浊点的相分离以获得液 - 液 - 固分数; 和v）回收含有目标多肽的富含表面活性剂的顶相。

    Fermentation processes and compositions
    68.
    发明申请
    Fermentation processes and compositions 审中-公开
    发酵过程和组成

    公开(公告)号：US20040063184A1

    公开(公告)日：2004-04-01

    申请号：US10459143

    申请日：2003-06-10

    Applicant: Novozymes North America, Inc.

    Inventor： Varvara Grichko

    IPC: C12P007/06 ,  C12P019/02

    CPC classification number: C12N1/38 ,  C12P7/06 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

    Abstract: The present invention provides improved fermentation processes, including for use in an ethanol production process. The improved fermentation processes include applying esterases (such as, lipases, phospholipases and cutinases), laccases, phytases and/or proteases to a fermentation process. The improved fermentation process may also involve the addition of various growth stimulators for the fermenting microorganisms, including vitamins and mineral.

    Abstract translation: 本发明提供改进的发酵方法，包括用于乙醇生产方法。 改进的发酵方法包括将酯酶（例如脂肪酶，磷脂酶和角质酶），漆酶，植酸酶和/或蛋白酶施用于发酵过程。 改进的发酵方法还可以包括添加用于发酵微生物的各种生长刺激剂，包括维生素和矿物质。

    De-inking of waste paper
    69.
    发明申请
    De-inking of waste paper 审中-公开
    废纸的脱墨

    公开(公告)号：US20040016522A1

    公开(公告)日：2004-01-29

    申请号：US10413335

    申请日：2003-04-14

    Applicant: Novozymes North America, Inc.

    Inventor： Neal Franks ,  Kelly Page

    IPC: D21B001/08 ,  D21B001/32 ,  D21C005/02

    CPC classification number: D21C5/027 ,  Y02W30/648

    Abstract: In the production of pulp and paper from starch-containing paper, the deinking effect can be improved by including treatment with both a starch-degrading enzyme and a pectate lyase. The process comprises enzyme treatment during or after disintegration of the paper to produce pulp, followed by separation of ink particles.

    Abstract translation: 在含淀粉纸的纸浆和纸的生产中，通过包括淀粉降解酶和果胶酸裂解酶的处理可以改善脱墨效果。 该方法包括在纸分解期间或之后的酶处理以产生纸浆，随后分离油墨颗粒。

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