公开(公告)号：US20120289600A1

公开(公告)日：2012-11-15

申请号：US13554429

申请日：2012-07-20

Applicant: Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

Inventor： Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

IPC: A61K31/202 ,  A61P3/06 ,  A23D9/00 ,  A61P29/00

CPC classification number: C12P7/6427 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/815 ,  C12P7/6472

Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, in the total oil fraction are described. These strains over-express heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases and C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases. Preferred gene knockouts are also described. Production host cells, methods for producing EPA within said host cells, and products comprising EPA from the optimized Yarrowia lipolytica strains are claimed.

Abstract translation: 描述了能够在总油馏分中产生大于50重量％的二十碳五烯酸[EPA]（ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株过表达异源＆Dgr。9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱氢酶，Dgr。12去饱和酶和C16 / 18延伸酶，以及任选的过表达二酰基甘油胆碱磷酸转移酶。 还描述了优选的基因敲除。 要求生产宿主细胞，在所述宿主细胞内生产EPA的方法，以及来自优化的解脂耶氏酵母菌株的包含EPA的产品。

公开(公告)号：US08148121B2

公开(公告)日：2012-04-03

申请号：US12512429

申请日：2009-07-30

Applicant: Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu ,  Hongxiang Zhang

Inventor： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu ,  Hongxiang Zhang

IPC: C12P7/64 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C12N9/0083 ,  C12N9/0071 ,  C12P7/6427 ,  C12Y114/19003

Abstract: The present invention relates to Δ6 desaturases, which have the ability to convert linoleic acid [“LA”; 18:2 ω-6] to γ-linolenic acid [“GLA”; 18:3 ω-6] and/or α-linolenic acid [“ALA”; 18:3 ω-3] to stearidonic acid [“STA”; 18:4 ω-3]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ6 desaturases, along with methods of making long-chain polyunsaturated fatty acids [“PUFAs”] using these Δ6 desaturases in oleaginous yeast, are disclosed.

Abstract translation: 本发明涉及具有转化亚油酸[“LA”; 18：2ω-6]与γ-亚麻酸[“GLA” 18：3ω-6]和/或α-亚麻酸[“ALA”; 18：3ω-3]至十八碳四烯酸[“STA”; 18：4ω-3]。 公开了分离的核酸片段和包含编码“Dgr”6去饱和酶的片段的重组构建体以及使用这些＆Dgr。6去饱和酶在含油酵母中制备长链多不饱和脂肪酸的方法。

公开(公告)号：US20110274787A9

公开(公告)日：2011-11-10

申请号：US12641929

申请日：2009-12-18

Applicant: Ethel Noland Jackson ,  David Richard Short ,  Dongming Xie ,  Zhixiong Xue ,  Quinn Qun Zhu

Inventor： Ethel Noland Jackson ,  David Richard Short ,  Dongming Xie ,  Zhixiong Xue ,  Quinn Qun Zhu

IPC: A23D7/02 ,  A23D9/02

CPC classification number: C12P7/6427

Abstract: Methods for the production of omega-3 and/or omega-6 fatty acids in oleaginous yeasts grown on a fermentable carbon source selected from the group consisting of invert sucrose, glucose, fructose and combinations of these, provided that glucose is used in combination with invert sucrose and/or fructose. Specifically, methods are provided for production of linoleic acid, eicosadienoic acid, gamma-linolenic acid, dihomo-gamma-linolenic acid, arachidonic acid, n-6 docosapentaenoic acid, α-linolenic acid, stearidonic acid, eicosatrienoic acid, eicosatetraenoic acid, eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid.

Abstract translation: 在选自蔗糖，葡萄糖，果糖及其组合的可发酵碳源上生长的油质酵母中生产ω-3和/或ω-6脂肪酸的方法，其中葡萄糖与 反转蔗糖和/或果糖。 具体地说，提供了用于生产亚油酸，二十碳二烯酸，γ-亚麻酸，二高-γ-亚麻酸，花生四烯酸，n-6二十二碳五烯酸，α-亚麻酸，十八碳四烯酸，二十碳三烯酸，二十碳四烯酸，二十碳五烯酸 酸，二十二碳五烯酸和二十二碳六烯酸。

公开(公告)号：US08049062B2

公开(公告)日：2011-11-01

申请号：US11992899

申请日：2006-11-16

Applicant: Howard Glenn Damude ,  Brian McGonigle ,  Quinn Qun Zhu ,  Zhixiong Xue

Inventor： Howard Glenn Damude ,  Brian McGonigle ,  Quinn Qun Zhu ,  Zhixiong Xue

IPC: A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N5/14

CPC classification number: C12N15/8247 ,  C11B1/025 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.

Abstract translation: 分离的核酸片段和包含编码Δ-9延伸酶的这种片段的重组构建物以及在植物中使用这些δ-9延长酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US07863502B2

公开(公告)日：2011-01-04

申请号：US11876115

申请日：2007-10-22

Applicant: Howard Damude ,  Quinn Qun Zhu ,  Zhixiong Xue

Inventor： Howard Damude ,  Quinn Qun Zhu ,  Zhixiong Xue

IPC: A01H5/00 ,  C12N15/82 ,  C12N5/10 ,  C07H21/04

CPC classification number: C12N15/8247 ,  A23K20/158 ,  A23L33/12 ,  C12N9/0083

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-8 desaturases in plants and oleaginous yeast.

Abstract translation: 分离的核酸片段和包含编码δ-8去饱和酶的片段的重组构建物以及使用这些δ-8去饱和酶在植物和油质酵母中制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20100159558A1

公开(公告)日：2010-06-24

申请号：US12637877

申请日：2009-12-15

Applicant: Quinn Qun Zhu ,  Zhixiong Xue

Inventor： Quinn Qun Zhu ,  Zhixiong Xue

IPC: C12N9/00 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N1/13

CPC classification number: C12P7/6427 ,  C12N9/93 ,  C12P7/6472

Abstract: Described are methods of reducing the amount of byproduct organic acids during fermentation of an organism, based on expression of a heterologous malonyl-CoA synthetase. A polyunsaturated fatty acid [“PUFA”]-producing strain of the oleaginous yeast Yarrowia lipolytica was engineered to express a heterologous malonyl-CoA synthetase gene. The expression did not effect the production of PUFAs, but did result in a reduced amount of malonates when compared to the amount of malonates produced in the parental strain not expressing malonyl-CoA synthetase.

Abstract translation: 描述了基于异源丙二酰辅酶A合成酶的表达减少生物体发酵期间副产物有机酸的量的方法。 将多油不饱和脂肪酸[“PUFA”]生产菌株的油质酵母解脂耶氏酵母解脂工程化以表达异源丙二酰辅酶A合成酶基因。 该表达不影响PUFA的产生，但与不表达丙二酰辅酶A合成酶的亲本菌株中产生的丙二酸的量相比，确实导致了丙二酸的量减少。

公开(公告)号：US07678560B2

公开(公告)日：2010-03-16

申请号：US11748629

申请日：2007-05-15

Applicant: Howard Glenn Damude ,  Dana M. Walters Pollak ,  Zhixiong Xue ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Dana M. Walters Pollak ,  Zhixiong Xue ,  Quinn Qun Zhu

IPC: C12N1/00 ,  C12N15/74 ,  C07H21/04

CPC classification number: A23L1/182 ,  A23K10/30 ,  A23L7/196 ,  A23L19/03 ,  A23V2002/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y114/19

Abstract: The present invention relates to a Δ5 desaturase, which has the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this Δ5 desaturase in oleaginous yeast are disclosed.

Abstract translation: 本发明涉及一种具有将二硫-γ-亚麻酸（DGLA; 20：3ω-6）转化成花生四烯酸（ARA; 20：4ω-6）和/或二十碳四烯酸 酸（ETA; 20：4ω-3）至二十碳五烯酸（EPA; 20：5ω-3）。 公开了分离的核酸片段和重组构建体，其包含编码“Dgr。5去饱和酶”的片段以及在含油酵母中使用该Dgr。5去饱和酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20090280996A1

公开(公告)日：2009-11-12

申请号：US12469208

申请日：2009-05-20

Applicant: Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

Inventor： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

IPC: C40B30/04 ,  C12N15/11 ,  C12N9/00 ,  C12Q1/68

CPC classification number: C12P7/6427 ,  C12N9/0083 ,  C12P7/6472

Abstract: The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力的Delta17去饱和酶（即将花生四烯酸[20：4，ARA]转化为二十碳五烯酸[20：5，EPA]） 。 公开了分离的核酸片段和包含编码Δ17去饱和酶的片段的重组构建物以及使用这些Delta17去饱和酶在含油酵母中制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20090253188A1

公开(公告)日：2009-10-08

申请号：US12408860

申请日：2009-03-23

Applicant: Quinn Qun Zhu ,  Zhixiong Xue

Inventor： Quinn Qun Zhu ,  Zhixiong Xue

IPC: C12P7/64 ,  C12N9/02 ,  C12N5/10 ,  C12N1/19 ,  C07H21/04

CPC classification number: C12N9/0083

Abstract: Described here are Δ4 desaturases that convert all-cis-7,10,13,16,19-docosapentaenoic acid [“DPA”; 22:5 ω-3] to docosahexaenoic acid [“DHA”; 22:6 ω-3], with secondary activity in converting docosatetraenoic acid [“DTA”; 22:4 ω-6] to all-cis-4,7,10,13,16-docosapentaenoic acid [“DPAn-6”; 22:5 ω-6]. Also, described here are isolated nuclei acid fragments and recombinant constructs comprising such fragments encoding Δ4 desaturases as well as methods of making long chain polyunsaturated fatty acids [“PUFAs”] using this Δ4 desaturase in oleaginous yeast.

Abstract translation: 这里描述的是转化全顺式-7,10,13,16,19-二十二碳五烯酸[“DPA”; 22：5ω-3]至二十二碳六烯酸[“DHA”; 22：6ω-3]，其二次活性转化为二十二碳四烯酸[“DTA”; 22：4ω-6]至全顺式-4,7,10,13,16-二十二碳五烯酸[“DPAn-6”; 22：5ω-6]。 此外，这里描述的是分离的核酸片段和包含编码Δ4去饱和酶的片段的重组构建体以及使用该Delta4去饱和酶在含油酵母中制备长链多不饱和脂肪酸[“PUFA”]的方法。

公开(公告)号：US20090093543A1

公开(公告)日：2009-04-09

申请号：US12244822

申请日：2008-10-03

Applicant: Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

Inventor： Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

IPC: A61K31/202 ,  C12N1/15 ,  A61P9/10 ,  A61P35/00 ,  A23D9/00 ,  A61P9/12 ,  C12P7/64

CPC classification number: C12P7/6427 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/815 ,  C12P7/6472

Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, in the total oil fraction are described. These strains over-express heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases and C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases. Preferred gene knockouts are also described. Production host cells, methods for producing EPA within said host cells, and products comprising EPA from the optimized Yarrowia lipolytica strains are claimed.

Abstract translation: 描述了能够在总油馏分中产生大于50重量％的二十碳五烯酸[“EPA”]，ω-3多不饱和脂肪酸的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株过表达异源Delta9延伸酶，Δ8去饱和酶，Δ5去饱和酶，Δ17去饱和酶，Δ12去饱和酶和C16 / 18延长酶，以及任选地过表达的二酰基甘油胆碱磷酸转移酶。 还描述了优选的基因敲除。 要求生产宿主细胞，在所述宿主细胞内生产EPA的方法，以及来自优化的解脂耶氏酵母菌株的包含EPA的产品。