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    Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
    2.
    发明授权
    Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production 有权
    优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产

    公开(公告)号：US08323935B2

    公开(公告)日：2012-12-04

    申请号：US12244822

    申请日：2008-10-03

    申请人： Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    发明人： Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    IPC分类号： C12P7/64 ,  C07D293/10

    CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/815 ,  C12P7/6472

    摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, in the total oil fraction are described. These strains over-express heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases and C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases. Preferred gene knockouts are described, as are methods for producing EPA within the host cells and products comprising EPA from the optimized Yarrowia lipolytica strains.

    摘要翻译： 描述了能够在总油馏分中产生大于50重量％的二十碳五烯酸[EPA]（ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株过表达异源＆Dgr。9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱氢酶，Dgr。12去饱和酶和C16 / 18延伸酶，以及任选的过表达二酰基甘油胆碱磷酸转移酶。 描述优选的基因敲除，以及在宿主细胞内生产EPA的方法以及来自优化的解脂耶氏酵母菌株的包含EPA的产物的方法。

    HIGH EICOSAPENTAENOIC ACID OILS FROM IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA
    4.
    发明申请
    HIGH EICOSAPENTAENOIC ACID OILS FROM IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA 有权
    来自改良的YARROWIA LIPOLYTICA优化菌株的高选择性酸性油

    公开(公告)号：US20100317735A1

    公开(公告)日：2010-12-16

    申请号：US12814880

    申请日：2010-06-14

    申请人： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    发明人： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    IPC分类号： A61K31/202 ,  A61P9/12 ,  A61P3/10 ,  A61P3/06 ,  A61P29/00 ,  A23L1/30 ,  A23K1/00 ,  A23D9/02 ,  C12N1/19

    CPC分类号： C12N9/1029 ,  A21D2/165 ,  A23K20/158 ,  C12N9/00 ,  C12N9/13 ,  C12N9/93 ,  C12P7/6427 ,  C12P7/6472 ,  C12P7/6481

    摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

    摘要翻译： 描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。 除了其他异源＆Dgr。9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱饱和酶，Dgr。12去饱和酶，C16外，这些菌株过度表达至少一种Dgr 9延伸酶/ / 18延伸酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 该菌株具有至少一种过氧化物酶体生物发生因子蛋白质敲除。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。

    DELTA 17 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS
    6.
    发明申请
    DELTA 17 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS 有权
    DELTA 17去饱和及其在制备聚氨酯脂肪酸中的应用

    公开(公告)号：US20080125326A1

    公开(公告)日：2008-05-29

    申请号：US11779915

    申请日：2007-07-19

    申请人： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

    发明人： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

    IPC分类号： C12P17/08 ,  C07H21/04 ,  C12N1/00 ,  C12N1/12 ,  C12Q1/68 ,  C40B30/04 ,  C12N1/20 ,  C12N5/06 ,  C12N9/00

    CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12P7/6472

    摘要： The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in oleaginous yeast are disclosed.

    摘要翻译： 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力的Delta17去饱和酶（即将花生四烯酸[20：4，ARA]转化为二十碳五烯酸[20：5，EPA]） 。 公开了分离的核酸片段和包含编码Δ17去饱和酶的片段的重组构建物以及使用这些Delta17去饱和酶在含油酵母中制备长链多不饱和脂肪酸（PUFA）的方法。

    DELTA 17 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS
    8.
    发明申请
    DELTA 17 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS 审中-公开
    DELTA 17去饱和及其在制备聚氨酯脂肪酸中的应用

    公开(公告)号：US20130011846A1

    公开(公告)日：2013-01-10

    申请号：US13535775

    申请日：2012-06-28

    申请人： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

    发明人： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

    IPC分类号： C12N9/02 ,  C40B20/00 ,  C12Q1/68 ,  C12N15/53

    CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12P7/6472

    摘要： The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in oleaginous yeast are disclosed.

    摘要翻译： 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力（即将花生四烯酸[20:4，ARA]转化为二十碳五烯酸[20:5，EPA ]）。 公开了分离的核酸片段和重组构建体，其包含编码＆amp; 17去饱和酶的这种片段以及使用这些在油性酵母中的这些＆Dgr.17去饱和酶制备长链多不饱和脂肪酸（PUFA）的方法。

    Δ17 desaturase and its use in making polyunsaturated fatty acids
    9.
    发明授权
    Δ17 desaturase and its use in making polyunsaturated fatty acids 有权
    去饱和酶及其在制备多不饱和脂肪酸中的应用

    公开(公告)号：US08241884B2

    公开(公告)日：2012-08-14

    申请号：US12469208

    申请日：2009-05-20

    申请人： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

    发明人： Narendra S. Yadav ,  Zhixiong Xue ,  Quinn Qun Zhu

    IPC分类号： C12N1/16

    CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12P7/6472

    摘要： The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in oleaginous yeast are disclosed.

    摘要翻译： 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力（即将花生四烯酸[20:4，ARA]转化为二十碳五烯酸[20:5，EPA ]）。 公开了分离的核酸片段和重组构建体，其包含编码＆amp; 17去饱和酶的这种片段以及使用这些在油性酵母中的这些＆Dgr.17去饱和酶制备长链多不饱和脂肪酸（PUFA）的方法。