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    Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
    4.
    发明授权
    Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production 有权
    优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产

    公开(公告)号：US08323935B2

    公开(公告)日：2012-12-04

    申请号：US12244822

    申请日：2008-10-03

    申请人： Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    发明人： Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    IPC分类号： C12P7/64 ,  C07D293/10

    CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/815 ,  C12P7/6472

    摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, in the total oil fraction are described. These strains over-express heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases and C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases. Preferred gene knockouts are described, as are methods for producing EPA within the host cells and products comprising EPA from the optimized Yarrowia lipolytica strains.

    摘要翻译： 描述了能够在总油馏分中产生大于50重量％的二十碳五烯酸[EPA]（ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株过表达异源＆Dgr。9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱氢酶，Dgr。12去饱和酶和C16 / 18延伸酶，以及任选的过表达二酰基甘油胆碱磷酸转移酶。 描述优选的基因敲除，以及在宿主细胞内生产EPA的方法以及来自优化的解脂耶氏酵母菌株的包含EPA的产物的方法。

    PENTOSE PHOSPHATE PATHWAY UPREGULATION TO INCREASE PRODUCTION OF NON-NATIVE PRODUCTS OF INTEREST IN TRANSGENIC MICROORGANISMS
    6.
    发明申请
    PENTOSE PHOSPHATE PATHWAY UPREGULATION TO INCREASE PRODUCTION OF NON-NATIVE PRODUCTS OF INTEREST IN TRANSGENIC MICROORGANISMS 审中-公开
    PENTOSE磷酸盐路径升级以增加转基因微生物中非生物利益产品的生产

    公开(公告)号：US20110244512A1

    公开(公告)日：2011-10-06

    申请号：US13074069

    申请日：2011-03-29

    申请人： Seung-Pyo Hong ,  Zhixiong Xue ,  Quinn Qun Zhu

    发明人： Seung-Pyo Hong ,  Zhixiong Xue ,  Quinn Qun Zhu

    IPC分类号： C12P1/00 ,  C12N1/00 ,  C12N1/13 ,  C12N1/19 ,  C12N1/15

    CPC分类号： C12P7/6427 ,  C12N9/0006 ,  C12N9/18 ,  C12N15/52 ,  C12P7/6472 ,  C12P7/66 ,  C12P23/00 ,  C12P33/00

    摘要： Coordinately regulated over-expression of the genes encoding glucose 6-phosphate dehydrogenase [“G6PDH”] and 6-phospho-gluconolactonase [“6PGL”] in transgenic strains of the oleaginous yeast, Yarrowia lipolytica, comprising a functional polyunsaturated fatty acid [“PUFA”] biosynthetic pathway, resulted in increased production of PUFAs and increased total lipid content in the Yarrowia cells. This is achieved by increased cellular availability of the reduced form of nicotinamide adenine dinucleotide phosphate [“NADPH”], an important reducing equivalent for reductive biosynthetic reactions, within the transgenic microorganism.

    摘要翻译： 协调调节编码葡萄糖6-磷酸脱氢酶[“G6PDH”]和6-磷酸 - 葡糖酸半乳糖醛酸酶[“6PGL”]的基因在含油酵母，解脂耶氏酵母的转基因菌株中的过表达，其包含功能性多不饱和脂肪酸[“PUFA “]生物合成途径导致了PUFA的生产增加，并增加了耶氏酵母细胞中的总脂质含量。 这是通过增加转基因微生物中烟酰胺腺嘌呤二核苷酸磷酸盐还原形式的细胞可利用度[“NADPH”]，这是还原性生物合成反应的重要还原等价物。

    HIGH EICOSAPENTAENOIC ACID OILS FROM IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA
    8.
    发明申请
    HIGH EICOSAPENTAENOIC ACID OILS FROM IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA 有权
    来自改良的YARROWIA LIPOLYTICA优化菌株的高选择性酸性油

    公开(公告)号：US20100317735A1

    公开(公告)日：2010-12-16

    申请号：US12814880

    申请日：2010-06-14

    申请人： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    发明人： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    IPC分类号： A61K31/202 ,  A61P9/12 ,  A61P3/10 ,  A61P3/06 ,  A61P29/00 ,  A23L1/30 ,  A23K1/00 ,  A23D9/02 ,  C12N1/19

    CPC分类号： C12N9/1029 ,  A21D2/165 ,  A23K20/158 ,  C12N9/00 ,  C12N9/13 ,  C12N9/93 ,  C12P7/6427 ,  C12P7/6472 ,  C12P7/6481

    摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

    摘要翻译： 描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。 除了其他异源＆Dgr。9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱饱和酶，Dgr。12去饱和酶，C16外，这些菌株过度表达至少一种Dgr 9延伸酶/ / 18延伸酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 该菌株具有至少一种过氧化物酶体生物发生因子蛋白质敲除。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。