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公开(公告)号：US07541455B2
公开(公告)日：2009-06-02
申请号：US11018897
申请日：2004-12-21
CPC分类号： C07H21/00 , B01J2219/00452 , B01J2219/00572 , B01J2219/00576 , B01J2219/00722 , C40B40/06
摘要： A method for preparing a nucleic acid probe is provided. The method comprises forming an activated cytosine or cytidine by a bisulfite catalyzed reaction; and covalently linking a reporter molecule to the activated cytosine or cytidine, wherein said activating step, said covalently linking step, or both are conducted in the presence of microwave energy. Also provided by the invention are nucleic acid probes.
摘要翻译： 提供了制备核酸探针的方法。 该方法包括通过亚硫酸氢盐催化反应形成活化的胞嘧啶或胞苷; 并将报告分子与活化的胞嘧啶或胞苷共价连接，其中所述活化步骤，所述共价连接步骤或两者均在微波能量存在下进行。 本发明还提供了核酸探针。
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公开(公告)号：US20050158770A1
公开(公告)日：2005-07-21
申请号：US11018897
申请日：2004-12-21
CPC分类号： C07H21/00 , B01J2219/00452 , B01J2219/00572 , B01J2219/00576 , B01J2219/00722 , C40B40/06
摘要： A method for preparing a nucleic acid probe is provided. The method comprises forming an activated cytosine or cytidine by a bisulfite catalyzed reaction; and covalently linking a reporter molecule to the activated cytosine or cytidine, wherein said activating step, said covalently linking step, or both are conducted in the presence of microwave energy. Also provided by the invention are nucleic acid probes.
摘要翻译： 提供了制备核酸探针的方法。 该方法包括通过亚硫酸氢盐催化反应形成活化的胞嘧啶或胞苷; 并将报告分子与活化的胞嘧啶或胞苷共价连接，其中所述活化步骤，所述共价连接步骤或两者均在微波能量存在下进行。 本发明还提供了核酸探针。
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63.发明申请Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest 有权酶催化的金属沉积用于增强感兴趣的分析物的检测公开(公告)号：US20050100976A1
公开(公告)日：2005-05-12
申请号：US11015646
申请日：2004-12-20
IPC分类号： C07F9/09 , C07F9/655 , C07H15/20 , C07H15/24 , C07H15/26 , G01N33/58 , C12Q1/68 , G01N33/53 , G01N33/537 , G01N33/543
CPC分类号： C07F9/093 , C07F9/65517 , C07F9/65522 , C07H15/20 , C07H15/24 , C07H15/26 , G01N33/581
摘要： The invention is directed to enhanced methods for detecting an analyte of interest in situ, by immunoassay, or by hybridization comprising binding an enzyme-labeled conjugate molecule to an analyte of interest in the presence of a redox-inactive reductive species and a soluble metal ion. The enzyme catalyzes the conversion of the inactive reductive species to an active reducing agent, which in turn reduces the metal ion to a metal atom thereby providing an enhanced means of detecting the analyte via metal deposition.
摘要翻译： 本发明涉及通过免疫测定或通过杂交来检测目的分析物的增强方法，包括在氧化还原惰性还原物质和可溶性金属离子存在下将酶标记的缀合物分子与目的分析物结合 。 酶催化无活性还原物质转化为活性还原剂，其又将金属离子还原成金属原子，从而提供通过金属沉积检测分析物的增强方法。
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公开(公告)号：US06503528B1
公开(公告)日：2003-01-07
申请号：US09444478
申请日：1999-11-19
IPC分类号： A61F202
CPC分类号： A61K9/2031 , A61K9/2018
摘要： The invention relates to a bioerodible polymeric composition, a method of making such a composition, and a controlled delivery device comprising the composition.
摘要翻译： 本发明涉及生物可蚀解聚合物组合物，制备这种组合物的方法以及包含该组合物的受控递送装置。
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65.发明授权Fluoroether compositions and methods for inhibiting their degradation in the presence of a Lewis acid 有权氟醚组合物和在路易斯酸存在下抑制其降解的方法公开(公告)号：US06288127B1
公开(公告)日：2001-09-11
申请号：US09447853
申请日：1999-11-23
申请人： Christopher Bieniarz , Steve H. Chang , Keith R. Cromack , Shuyen L. Huang , Toshikazu Kawai , Manami Kobayashi , David Loffredo , Rajagopalan Raghavan , Earl R. Speicher , Honorate A. Stelmach
发明人： Christopher Bieniarz , Steve H. Chang , Keith R. Cromack , Shuyen L. Huang , Toshikazu Kawai , Manami Kobayashi , David Loffredo , Rajagopalan Raghavan , Earl R. Speicher , Honorate A. Stelmach
IPC分类号： A61K3108
CPC分类号： A61K47/02 , A61K9/0019 , A61K31/08 , A61K47/10 , A61K47/12 , A61K47/14 , Y10S514/816
摘要： The present invention relates to an anesthetic composition containing a fluoroether compound and a physiologically acceptable Lewis acid inhibitor. This composition exhibits improved stability and does not readily degrade in the presence of a Lewis acid.
摘要翻译： 本发明涉及含有氟醚化合物和生理上可接受的路易斯酸抑制剂的麻醉组合物。 该组合物表现出改进的稳定性，并且在路易斯酸存在下不容易降解。
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公开(公告)号：US06245949B1
公开(公告)日：2001-06-12
申请号：US09587414
申请日：2000-06-01
IPC分类号： C07C4101
CPC分类号： C07C41/28 , C07C41/50 , C07C43/123 , C07C43/313
摘要： A method for fluoromethylating halogenated alcohols. The method includes the step of providing an alpha-halogenated alcohol of the formula R1C(CX3)2OH, wherein R1 is selected from the group consisting of hydrogen and alkyl groups. The alpha-halogenated alcohol is reacted with a first compound of the formula CH2(OR2)2 in the presence of an acid catalyst to form an acetal. The resulting acetal is then chlorinated with a chlorinating agent to form a chloride compound of the formula R1C(CX3)2OCH2Cl. The chloride compound is then converted to a fluoride compound of the formula R1C(CX3)2OCH2F using a fluorinating agent.
摘要翻译： 氟代甲基化卤代醇的方法。 该方法包括提供式R1C（CX3）2OH的α-卤代醇的步骤，其中R 1选自氢和烷基。 在酸催化剂存在下，使α-卤代醇与式CH 2（OR 2）2的第一化合物反应，形成缩醛。 然后将得到的缩醛用氯化剂氯化以形成式R 1 C（CX 3）2 OCH 2 Cl的氯化物化合物。 然后使用氟化剂将氯化物化合物转化为式R1C（CX3）2OCH2F的氟化物。
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67.发明授权Polymeric fluorophores enhanced by moieties providing a hydrophobic and conformationally restrictive microenvironment 失效通过提供疏水和构象限制性微环境的部分增强的聚合物荧光团
公开(公告)号：US5994143A
公开(公告)日：1999-11-30
申请号：US595092
申请日：1996-02-01
IPC分类号： C09B69/10 , G01N21/78 , G01N33/533 , G01N33/00 , C09B5/00
CPC分类号： C09B69/10 , G01N33/533 , Y10S436/80 , Y10T436/14 , Y10T436/141111 , Y10T436/17 , Y10T436/18
摘要： Fluorescent conjugates suitable for use in flow cytometry and other biological applications. The fluorescent conjugates comprise an antibody having a polymeric dye bound thereto. The polymeric dye is preferably enhanced by a hydrophobic and conformationally restrictive moiety either bound thereto or in close association therewith. The hydrophobic and conformationally restrictive moiety is preferably derived from a cyclodextrin. The polymeric dye comprises a polymeric entity having signal-generating groups, such as aminostyryl pyridinium dye residues attached thereto. The fluorescent conjugates exhibit exceptional stability characteristics and avoid many of the problems of energy transfer, bio-conjugability, and solubility.
摘要翻译： 适用于流式细胞术和其他生物应用的荧光偶联物。 荧光共轭物包含具有与其结合的聚合染料的抗体。 聚合染料优选通过与其结合或与其紧密结合的疏水性和构象限制性部分增强。 疏水和构象限制性部分优选衍生自环糊精。 聚合染料包括具有信号产生基团的聚合物，例如与其连接的氨基苯乙烯基吡啶鎓染料残基。 荧光共轭物表现出优异的稳定性特征，并避免能量转移，生物共轭性和溶解度的许多问题。
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公开(公告)号：US09291597B2
公开(公告)日：2016-03-22
申请号：US13805983
申请日：2011-07-01
申请人： Rui Hong , Hong Wang , Mark Lefever , Jan Froehlich , Christopher Bieniarz , Brian Kelly , Phillip Miller
发明人： Rui Hong , Hong Wang , Mark Lefever , Jan Froehlich , Christopher Bieniarz , Brian Kelly , Phillip Miller
CPC分类号： G01N33/6848 , G01N27/62
摘要： Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags having the same chemical structure but different masses. Mass codes produced by ionizing the mass tags are detected and/or quantified using mass spectrometry. The method can be used for multiplexed detection of multiple targets in a particular sample. In some embodiments, a map divided into sections representing sections of the tissue sample may be prepared, with the map sections including data corresponding to quantification data wherein the size of a mass peak is determined and correlated with the amount of a target for the corresponding tissue sample section.
摘要翻译： 本文公开的具体公开的实施方案涉及使用一种或多种各种质量标签，其可以通过直接或间接的酶催化转化特异性沉积在靶上，以提供用于鉴定组织样品中靶标的方法。 质量标签可以用稳定同位素标记，以产生具有相同化学结构但质量不同的质量标签。 使用质谱检测和/或定量通过电离质量标签产生的质量代码。 该方法可用于特定样品中多个目标的多路检测。 在一些实施例中，可以制备分成表示组织样本部分的部分的图，其中，图表部分包括对应于量化数据的数据，其中质量峰的大小被确定并与相应组织的靶的量相关联 样品部分。
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69.发明授权Substrates for chromogenic detection and methods of use in detection assays and kits 有权用于显色检测的底物和用于检测测定和试剂盒的方法公开(公告)号：US09127028B2
公开(公告)日：2015-09-08
申请号：US13809354
申请日：2011-08-12
IPC分类号： C07F9/12 , C12Q1/70 , C07F9/6503 , G01N21/78
CPC分类号： C07F9/65031 , G01N21/78
摘要： Embodiments of substrates and processes for chromogenic detection, and in particular pyrazolyl dihydrogen phosphate compounds, are disclosed.
摘要翻译： 公开了用于显色检测的底物和方法，特别是吡唑基二氢磷酸酯化合物的实施方案。
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公开(公告)号：US08486620B2
公开(公告)日：2013-07-16
申请号：US13066980
申请日：2011-04-29
CPC分类号： G01N33/581 , A61K47/6883 , A61K49/0058 , A61K49/0067 , C07K17/08 , G01N33/532 , G01N33/54353 , G01N33/544 , G01N33/545 , G01N33/56983 , G01N33/57492 , G01N33/582 , G01N33/587 , G01N33/588
摘要： Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.
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