公开(公告)号：US07511017B2

公开(公告)日：2009-03-31

申请号：US11182946

申请日：2005-07-18

申请人： Ying-Fei Wei ,  Jian Ni ,  Reiner L. Gentz ,  Steven M. Ruben

发明人： Ying-Fei Wei ,  Jian Ni ,  Reiner L. Gentz ,  Steven M. Ruben

IPC分类号： A61K38/16 ,  A61K38/00 ,  C07K14/435 ,  C07K14/00

CPC分类号： A61K31/522 ,  A61K31/66 ,  A61K39/395 ,  A61K45/06 ,  A61K48/00 ,  A61K2039/505 ,  C07K14/70578 ,  C07K16/2878 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2799/026 ,  A61K2300/00

摘要： The present invention relates to a novel human gene encoding a polypeptide which is a member of the TNF receptor family, and has now been found to bind TRAIL. More specifically, an isolated nucleic acid molecule is provided encoding a human polypeptide named tumor necrosis factor receptor-5, sometimes referred to as “TNFR-5” or “TR5,” and now referred to hereinafter as “TRAIL receptor without intracellular domain” or “TRID.” TRID polypeptides are also provided, as are vectors, host cells, and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists or antagonists of TRAIL polypeptide activity. Also provided are diagnostic and therapeutic methods utilizing such compositions.

摘要翻译： 本发明涉及编码作为TNF受体家族成员的多肽的新型人基因，现已发现其结合TRAIL。 更具体地，提供编码称为肿瘤坏死因子受体-5（有时称为“TNFR-5”或“TR5”）的人多肽的分离的核酸分子，以下在下文中称为“没有细胞内结构域的TRAIL受体”或 “TRID”。 还提供了TRID多肽，载体，宿主细胞以及用于制备它们的重组方法也是如此。 本发明还涉及用于鉴定TRAIL多肽活性的激动剂或拮抗剂的筛选方法。 还提供了利用这种组合物的诊断和治疗方法。

公开(公告)号：US07495084B2

公开(公告)日：2009-02-24

申请号：US10692730

申请日：2003-10-27

申请人： Jian Ni ,  Reiner L. Gentz ,  Steven M. Ruben

发明人： Jian Ni ,  Reiner L. Gentz ,  Steven M. Ruben

IPC分类号： C07K16/00

CPC分类号： C07K14/54

摘要： The present invention concerns a novel T1R-like ligand II protein. In particular, isolated nucleic acid molecules are provided encoding the T1R-like ligand II protein. T1R-like ligand II polypeptides are also provided, as are recombinant vectors and host cells for expressing the same.

摘要翻译： 本发明涉及一种新的T1R样配体II蛋白。 特别地，提供编码T1R样配体II蛋白质的分离的核酸分子。 还提供了T1R样配体II多肽，重组载体和用于表达它们的宿主细胞也是如此。

公开(公告)号：US07427492B1

公开(公告)日：2008-09-23

申请号：US08741095

申请日：1996-10-30

申请人： Jian Ni ,  Craig A. Rosen ,  Reiner L. Gentz

发明人： Jian Ni ,  Craig A. Rosen ,  Reiner L. Gentz

IPC分类号： C12N15/12 ,  C12N15/63 ,  C12N15/64 ,  C12N15/09 ,  C12N5/16 ,  C12N5/10 ,  C12N1/11 ,  C12N1/15 ,  C12N1/21

CPC分类号： C07K14/7151

摘要： The present invention relates to novel members of the Tumor Necrosis Factor family of receptors. The invention provides isolated nucleic acid molecules encoding a human TR2 receptor and two splice variants thereof. TR2 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of TR2 receptor activity. Also provided are diagnostic methods for detecting disease states related to the aberrant expression of TR2 receptors. Further provided are therapeutic methods for treating disease states related to aberrant proliferation and differentiation of cells which express the TR2 receptors.

摘要翻译： 本发明涉及肿瘤坏死因子家族受体的新成员。 本发明提供编码人TR2受体的分离的核酸分子及其两个剪接变体。 还提供TR2多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定TR2受体活性的激动剂和拮抗剂的筛选方法。 还提供了用于检测与TR2受体的异常表达相关的疾病状态的诊断方法。 还提供了治疗与表达TR2受体的细胞的异常增殖和分化相关的疾病状态的治疗方法。

公开(公告)号：US07098316B2

公开(公告)日：2006-08-29

申请号：US10314942

申请日：2002-12-10

申请人： Jian Ni ,  Craig A. Rosen ,  Reiner L. Gentz ,  Jeffrey Y. Su ,  Geoffrey Krissansen ,  Ping Feng

发明人： Jian Ni ,  Craig A. Rosen ,  Reiner L. Gentz ,  Jeffrey Y. Su ,  Geoffrey Krissansen ,  Ping Feng

IPC分类号： C07K16/00

CPC分类号： C07K14/70535 ,  A61K38/00 ,  C07K14/47 ,  C07K14/705 ,  C07K14/70503 ,  C12N9/6421 ,  C12N9/6475 ,  C12N9/6489 ,  C12N9/6491 ,  C12N2799/026

摘要： The present invention relates to novel human proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells and recombinant methods for producing the proteins of the invention. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human secreted proteins.

摘要翻译： 本发明涉及包含编码这种蛋白质的基因的编码区的新型人类蛋白质和分离的核酸。 还提供了用于产生本发明的蛋白质的载体，宿主细胞和重组方法。 本发明还涉及用于诊断和治疗与这些新型人分泌蛋白相关的病症的诊断和治疗方法。

公开(公告)号：US06903072B2

公开(公告)日：2005-06-07

申请号：US09345373

申请日：1999-07-01

申请人： Steven M. Ruben ,  Pablo Jimenez ,  D. Roxanne Duan ,  Mark A. Rampy ,  Donna Mendrick ,  Jun Zhang ,  Jian Ni ,  Paul A. Moore ,  Timothy A. Coleman ,  Joachim R. Gruber ,  Patrick J. Dillon ,  Reiner L. Gentz

发明人： Steven M. Ruben ,  Pablo Jimenez ,  D. Roxanne Duan ,  Mark A. Rampy ,  Donna Mendrick ,  Jun Zhang ,  Jian Ni ,  Paul A. Moore ,  Timothy A. Coleman ,  Joachim R. Gruber ,  Patrick J. Dillon ,  Reiner L. Gentz

IPC分类号： A61K38/00 ,  A61K48/00 ,  C07H21/00 ,  C07K14/50 ,  C12N5/02 ,  C12N15/18 ,  A61K38/18 ,  C12N1/21

CPC分类号： C07H21/00 ,  A61K38/00 ,  A61K48/00 ,  C07K14/50 ,  C12N2799/026 ,  Y10S514/925

摘要： This invention relates to newly identified polynucleotides, polypeptides encoded by such polynucleotides, the use of such polynucleotides and polypeptides, as well as the production of such polynucleotides and polypeptides. More particularly, the polypeptide of the present invention is a Keratinocyte Growth Factor, sometimes hereinafter referred to as “KGF-2” also formerly known as Fibroblast Growth Factor 12 (FGF-12). This invention further relates to the therapeutic use of KGF-2 to promote or accelerate wound healing. This invention also relates to novel mutant forms of KGF-2 that show enhanced activity, increased stability, higher yield or better solubility.

公开(公告)号：US06610829B2

公开(公告)日：2003-08-26

申请号：US09854549

申请日：2001-05-15

申请人： Jian Ni ,  Ping Feng ,  Patrick J. Dillon ,  Reiner L. Gentz ,  Joseph Merregaert ,  Patrick Smits

发明人： Jian Ni ,  Ping Feng ,  Patrick J. Dillon ,  Reiner L. Gentz ,  Joseph Merregaert ,  Patrick Smits

IPC分类号： C07K100

CPC分类号： C12Q1/6883 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/51 ,  C07K14/47 ,  C07K14/51 ,  C12N2799/026 ,  C12Q2600/156 ,  Y10T436/143333

摘要： A human ECM-1 polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptide for stimulating the differentiation in growth of osteoblasts and osteoclasts, which may be used to promote the healing of bone fractures and de novo bone formation, for osteoporosis, for and to promote angiogenesis. Antagonists to the polypeptide of the present invention are also disclosed which may be utilized to treat osteodystrophy, osteohypertrophy, osteoma, osteoblastoma and cancers. Diagnostic assays for identifying mutations in nucleic acid sequence encoding a polypeptide of the present invention.

摘要翻译： 公开了编码这种多肽的人ECM-1多肽和DNA（RNA），以及通过重组技术产生此类多肽的方法。 还公开了利用这种多肽刺激成骨细胞和破骨细胞生长分化的方法，其可用于促进骨折愈合和从头骨形成，用于骨质疏松症，促进血管生成。 还公开了本发明多肽的拮抗剂，其可用于治疗骨营养不良，骨质肥大，骨瘤，成骨细胞瘤和癌症。 用于鉴定编码本发明多肽的核酸序列中的突变的诊断测定。

公开(公告)号：US06461823B1

公开(公告)日：2002-10-08

申请号：US09448868

申请日：1999-11-24

申请人： Jian Ni ,  Craig A. Rosen ,  James G. Pan ,  Reiner L. Gentz ,  Vishva M. Dixit

发明人： Jian Ni ,  Craig A. Rosen ,  James G. Pan ,  Reiner L. Gentz ,  Vishva M. Dixit

IPC分类号： G01N33567

CPC分类号： C07K14/70578

摘要： The present invention relates to novel Death Domain Containing Receptor-4 (DR4) proteins which are members of the tumor necrosis factor (TNF) receptor family. In particular, isolated nucleic acid molecules are provided encoding the human DR4 proteins. DR4 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of DR4 activity.

摘要翻译： 本发明涉及作为肿瘤坏死因子（TNF）受体家族成员的新的含有死亡域的受体-4（DR4）蛋白。 特别地，提供编码人DR4蛋白的分离的核酸分子。 还提供了DR4多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定DR4活性的激动剂和拮抗剂的筛选方法。

公开(公告)号：US06433147B1

公开(公告)日：2002-08-13

申请号：US09565918

申请日：2000-05-05

申请人： Jian Ni ,  Craig A. Rosen ,  James G. Pan ,  Reiner L. Gentz ,  Vishva M. Dixit

发明人： Jian Ni ,  Craig A. Rosen ,  James G. Pan ,  Reiner L. Gentz ,  Vishva M. Dixit

IPC分类号： C07K14705

CPC分类号： C07K16/2878 ,  A61K38/00 ,  A61K39/39541 ,  C07K14/70578 ,  C07K14/7151 ,  C07K2319/30 ,  C12N2799/026 ,  A61K2300/00

摘要： The present invention relates to novel Death Domain Containing Receptor-4 (DR4) proteins which are members of the tumor necrosis factor (TNF) receptor family. In particular, isolated nucleic acid molecules are provided encoding the human DR4 proteins. DR4 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of DR4 activity and methods for using DR4 polynucleotides and polypeptides.

摘要翻译： 本发明涉及作为肿瘤坏死因子（TNF）受体家族成员的新的含有死亡域的受体-4（DR4）蛋白。 特别地，提供编码人DR4蛋白的分离的核酸分子。 还提供了DR4多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定DR4活性的激动剂和拮抗剂的筛选方法以及使用DR4多核苷酸和多肽的方法。

公开(公告)号：US06262233B1

公开(公告)日：2001-07-17

申请号：US09013896

申请日：1998-01-27

申请人： Reiner L. Gentz ,  Tsu-An Hsu ,  Craig A. Rosen ,  Jian Ni

发明人： Reiner L. Gentz ,  Tsu-An Hsu ,  Craig A. Rosen ,  Jian Ni

IPC分类号： C07K400

CPC分类号： C07K14/8114 ,  A61K38/00 ,  C07K2319/00

摘要： The present invention relates to a novel TFPI-3 protein which is a member of the tissue factor protease inhibitor family. In particular, isolated nucleic acid molecules are provided encoding human TFPI-3 proteins. TFPI-3 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of TFPI-3 activity. Also provided are diagnostic methods for detecting hemostasis system-related disorders and therapeutic methods for treating hemostatis system-related disorders.

摘要翻译： 本发明涉及作为组织因子蛋白酶抑制剂家族成员的新型TFPI-3蛋白。 特别地，提供编码人TFPI-3蛋白质的分离的核酸分子。 还提供TFPI-3多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定TFPI-3活性的激动剂和拮抗剂的筛选方法。 还提供了用于检测止血系统相关疾病的诊断方法和治疗止血系统相关疾病的治疗方法。

公开(公告)号：US5945321A

公开(公告)日：1999-08-31

申请号：US875272

申请日：1997-10-02

申请人： Jian Ni ,  Reiner L. Gentz ,  Mark D. Adams

发明人： Jian Ni ,  Reiner L. Gentz ,  Mark D. Adams

IPC分类号： C12N9/00 ,  C12N9/10

CPC分类号： C12N9/93 ,  C12Y603/02019 ,  C07K2319/00 ,  C12N2799/026

摘要： Human UCE 7, UCE 8 and UCE 9 polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods of utilizing such polypeptides for the treatment of the porliferation of malignant cells. Antagonists against such polypeptides and their uses as a therapeutic to treat Alzheimer's disease, atrophying skeletal muscle, African Swine Fever Virus and apoptotic cell death are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to mutations in UCE 7, 8 and 9 nucleic acid sequences and to the concentration of polypeptides encoded by such sequences.

摘要翻译： PCT No.PCT / US95 / 01250 Sec。 371 1997年10月2日第 102（e）日期1997年10月2日PCT提交1995年1月31日PCT公布。 出版物WO96 / 23410 PCT 日期1996年8月8日公开了人类UCE 7，UCE 8和UCE 9多肽和编码此类多肽的DNA（RNA）以及通过重组技术产生此类多肽的方法。 还公开了利用这些多肽来治疗恶性细胞增殖的方法。 还公开了抗这些多肽的拮抗剂及其作为治疗性阿尔茨海默氏病，萎缩性骨骼肌，非洲猪热病毒和凋亡性细胞死亡的治疗剂的用途。 还公开了用于检测与UCE7,8和9核酸序列中的突变有关的疾病的诊断测定和由这些序列编码的多肽的浓度。