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    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein, and method of producing optically active amino acid
    61.
    发明授权
    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein, and method of producing optically active amino acid 失效
    编码乙内酰脲的DNA，编码N-氨基甲酰基-L-氨基酸水解酶的DNA，重组DNA，转化细胞，产生蛋白质的方法以及制备光学活性氨基酸的方法

    公开(公告)号：US08460902B1

    公开(公告)日：2013-06-11

    申请号：US12035525

    申请日：2008-02-22

    申请人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    发明人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    IPC分类号： C12N15/55 ,  C12N9/80 ,  C12P13/04 ,  C12P13/22

    CPC分类号： C12N9/80 ,  C12N9/86 ,  C12P13/04 ,  C12P41/009 ,  C12Y305/01077 ,  C12Y305/02002

    摘要： The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence of SEQ ID NO:1. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence of SEQ ID NO:3.

    摘要翻译： 本发明提供了一种通过分离涉及将5-取代的乙内酰脲或N-氨基甲酰基氨基酸转化成5-取代的乙内酰脲或N-氨基甲酰基氨基酸的能力的乙内酰脲酶基因和N-氨基甲酰基-L-氨基酸水解酶基因从5-取代的乙内酰脲生产旋光性氨基酸的方法 具有上述能力的微杆菌属的微生物的旋光活性氨基酸，通过改善基因扩增和转录和翻译活性，从而制备重组体，其中产生的所需酶的量增加。 乙内酰脲酶基因是例如编码具有乙内酰脲酶活性的蛋白质的DNA，其具有SEQ ID NO：1的核苷酸序列。 N-氨基甲酰基-L-氨基酸水解酶基因是例如编码具有N-氨基甲酰基-L-氨基酸水解酶活性的蛋白质的DNA，其具有SEQ ID NO：3的核苷酸序列。

    METHOD FOR PRODUCING BETA-ALANYL-AMINO ACID OR DERIVATIVE THEREOF
    64.
    发明申请
    METHOD FOR PRODUCING BETA-ALANYL-AMINO ACID OR DERIVATIVE THEREOF 审中-公开
    生产β-氨基酸或其衍生物的方法

    公开(公告)号：US20110081678A1

    公开(公告)日：2011-04-07

    申请号：US12943509

    申请日：2010-11-10

    申请人： Rie Takeshita ,  Yasuaki Takakura ,  Shunichi Suzuki ,  Kenzo Yokozeki

    发明人： Rie Takeshita ,  Yasuaki Takakura ,  Shunichi Suzuki ,  Kenzo Yokozeki

    IPC分类号： C12P21/02 ,  C07H21/04 ,  C12N9/00 ,  C12N5/10

    CPC分类号： C12P21/02 ,  C07K5/0202 ,  C12N9/48

    摘要： The present invention provides a method for efficiently producing a β-alanyl-amino acid (e.g., carnosine) or derivative thereof. Specifically, it provides a method for producing a β-alanyl-amino acid (e.g., β-alanyl-histidine) or derivative thereof, by reacting a β-alanyl ester or a β-alanyl amide and an amino acid or derivative thereof in the presence of an enzyme or an enzyme-containing product that has an ability to form the β-alanyl-amino acid (e.g., β-alanyl-histidine) or derivative thereof from the β-alanyl ester or the β-alanyl amide and the amino acid (e.g., histidine) or derivative thereof. The present invention also provides a protein which is able to catalyze production of a β-alanyl-amino acid or derivative thereof from a β-alanyl ester or a β-alanyl amide and an amino acid or derivative thereof, and a polynucleotide encoding said protein.

    摘要翻译： 本发明提供了一种有效生产β-丙氨酰 - 氨基酸（如肌肽）或其衍生物的方法。 具体地说，本发明提供了一种通过使β-丙氨酰基酯或一种 - 丙氨酰基酰胺与氨基酸或氨基酸反应生产β-丙氨酰 - 氨基酸（例如， - 丙氨酰 - 组氨酸）或其衍生物的方法 其衍生物在酶或含酶产物的存在下具有从丙氨酰酯形成β-淀粉氨基酸（例如，β-丙氨酰 - 组氨酸）或其衍生物的能力，或 丙氨酰酰胺和氨基酸（如组氨酸）或其衍生物。 本发明还提供一种蛋白质，其能够催化β-氨基丙氨酰 - 氨基酸或其衍生物从β-丙氨酰酯或其 - 氨基酸或其衍生物及其衍生物的产生，以及多核苷酸 编码所述蛋白质。

    Transporter protein
    65.
    发明授权
    Transporter protein 失效
    转运蛋白

    公开(公告)号：US07851610B2

    公开(公告)日：2010-12-14

    申请号：US11946113

    申请日：2007-11-28

    申请人： Shunichi Suzuki ,  Kenzo Yokozeki ,  Peter Henderson

    发明人： Shunichi Suzuki ,  Kenzo Yokozeki ,  Peter Henderson

    IPC分类号： C07K1/00 ,  C07H21/04 ,  A61K39/00

    CPC分类号： C07K14/195

    摘要： A novel protein which has an activity to transport hydantoin compounds is described, as well as a recombinant expressing this transporter protein. From Microbacterium liquefaciens strain AJ3912, a novel gene was discovered to encode a protein which is able to transport hydantoin compounds. A recombinant with an excellent ability to uptake hydantoin compounds is obtained by introducing and expressing the novel gene, called mhp, using gene recombination techniques.

    摘要翻译： 描述了具有转运乙内酰脲化合物的活性的新型蛋白质，以及表达该转运蛋白的重组体。 从微型液化酵母菌株AJ3912开始，发现了一种能够转运乙内酰脲化合物的蛋白质的新基因。 通过使用基因重组技术引入和表达新的基因，称为mhp，获得具有优异吸收乙内酰脲化合物能力的重组体。

    Novel Transporter Protein
    66.
    发明申请
    Novel Transporter Protein 失效
    新型运载蛋白

    公开(公告)号：US20090253168A1

    公开(公告)日：2009-10-08

    申请号：US11946113

    申请日：2007-11-28

    申请人： Shunichi Suzuki ,  Kenzo Yokozeki ,  Peter Henderson

    发明人： Shunichi Suzuki ,  Kenzo Yokozeki ,  Peter Henderson

    IPC分类号： C12P21/02 ,  C12N15/31 ,  C12N1/21 ,  C12P13/04

    CPC分类号： C07K14/195

    摘要： A novel protein which has an activity to transport hydantoin compounds is described, as well as a recombinant expressing this transporter protein. From Microbacterium liquefaciens strain AJ3912, a novel gene was discovered to encode a protein which is able to transport hydantoin compounds. A recombinant with an excellent ability to uptake hydantoin compounds is obtained by introducing and expressing the novel gene, called mhp, using gene recombination techniques.

    摘要翻译： 描述了具有转运乙内酰脲化合物的活性的新型蛋白质，以及表达该转运蛋白的重组体。 从微型液化酵母菌株AJ3912开始，发现了一种能够转运乙内酰脲化合物的蛋白质的新基因。 通过使用基因重组技术引入和表达新的基因，称为mhp，获得具有优异吸收乙内酰脲化合物能力的重组体。

    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein and method of producing optically active amino acid
    67.
    发明授权
    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein and method of producing optically active amino acid 失效
    编码乙内酰脲的DNA，编码N-氨基甲酰-L-氨基酸水解酶的DNA，重组DNA，转化细胞，产生蛋白质的方法和产生旋光氨基酸的方法

    公开(公告)号：US07361490B2

    公开(公告)日：2008-04-22

    申请号：US11476572

    申请日：2006-06-29

    申请人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    发明人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    IPC分类号： C12P13/22 ,  C12P13/04 ,  C12N9/86

    CPC分类号： C12N9/80 ,  C12N9/86 ,  C12P13/04 ,  C12P41/009 ,  C12Y305/01077 ,  C12Y305/02002

    摘要： The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence set forth in SEQ ID NO:1 in the Sequence. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence set forth in SEQ ID NO:3 in the Sequence.

    摘要翻译： 本发明提供了一种通过分离涉及将5-取代的乙内酰脲或N-氨基甲酰基氨基酸转化成5-取代的乙内酰脲或N-氨基甲酰基氨基酸的能力的乙内酰脲酶基因和N-氨基甲酰基-L-氨基酸水解酶基因从5-取代的乙内酰脲生产旋光性氨基酸的方法 具有上述能力的微杆菌属的微生物的旋光活性氨基酸，通过改善基因扩增和转录和翻译活性，从而制备重组体，其中产生的所需酶的量增加。 乙内酰脲酶基因是例如编码具有乙内酰脲酶活性的蛋白质的DNA，其具有序列中SEQ ID NO：1所示的核苷酸序列。 N-氨基甲酰基-L-氨基酸水解酶基因是例如编码具有N-氨基甲酰基-L-氨基酸水解酶活性的蛋白质的DNA，其具有SEQ ID NO：3所示的核苷酸序列 序列。