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    Method for producing dipeptides
    1.
    发明授权
    Method for producing dipeptides 失效
    二肽生产方法

    公开(公告)号：US07754466B2

    公开(公告)日：2010-07-13

    申请号：US10476885

    申请日：2002-07-26

    申请人： Kenzo Yokozeki ,  Sonoko Suzuki

    发明人： Kenzo Yokozeki ,  Sonoko Suzuki

    IPC分类号： C12N1/20 ,  C12P21/06 ,  G01N33/58

    CPC分类号： C12P21/02 ,  C07K5/06026 ,  C12N9/48

    摘要： The present invention provides a method of producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide from an amino acid ester and an amino acid, microbial cells separated from the culture, or treated microbial cell product.

    摘要翻译： 本发明提供了一种通过工业上有利和简单的途径以低成本获得的原料制备二肽的方法。 通过使用具有从氨基酸酯和氨基酸产生二肽的能力的微生物培养物，从培养物分离的微生物细胞或经处理的微生物细胞产物，由氨基酸酯和氨基酸产生二肽。

    Method for producing tripeptides and/or peptides longer than tripeptides
    2.
    发明授权
    Method for producing tripeptides and/or peptides longer than tripeptides 失效
    生产长于三肽的三肽和/或肽的方法

    公开(公告)号：US07338780B2

    公开(公告)日：2008-03-04

    申请号：US10849814

    申请日：2004-05-21

    申请人： Kenzo Yokozeki ,  Sonoko Suzuki ,  Seiichi Hara ,  Isao Abe

    发明人： Kenzo Yokozeki ,  Sonoko Suzuki ,  Seiichi Hara ,  Isao Abe

    IPC分类号： C12P21/06 ,  C12N9/10 ,  C12N1/20 ,  C12H21/40

    CPC分类号： C07K5/06 ,  C12N9/16 ,  C12N9/52 ,  C12N9/80 ,  C12P21/02

    摘要： A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.

    摘要翻译： 公开了一种能够容易地，低成本地以高产率制备具有三个或更多个氨基酸残基的肽而不经过复合合成方法的方法。 提供了一种新颖的酶，其有效地从属于Empedobacter属或Sphingobacterium属的细菌产生肽。 该酶作用于羧基组分和胺组分以通过作用于羧基组分和胺组分形成具有三个或更多个氨基酸残基的肽。

    Mutant protein having the peptide-synthesizing activity
    4.
    发明申请
    Mutant protein having the peptide-synthesizing activity 审中-公开
    具有肽合成活性的突变蛋白

    公开(公告)号：US20060188976A1

    公开(公告)日：2006-08-24

    申请号：US11311467

    申请日：2005-12-20

    申请人： Rie Takeshita ,  Isao Abe ,  Masakazu Sugiyama ,  Kenzo Yokozeki ,  Seiichi Hara ,  Sonoko Suzuki ,  Shunichi Suzuki ,  Kunihiko Watanabe

    发明人： Rie Takeshita ,  Isao Abe ,  Masakazu Sugiyama ,  Kenzo Yokozeki ,  Seiichi Hara ,  Sonoko Suzuki ,  Shunichi Suzuki ,  Kunihiko Watanabe

    IPC分类号： C12N9/48 ,  C07H21/04 ,  C12P21/06 ,  C12N1/21 ,  C12N15/74

    CPC分类号： C07K14/00 ,  C07K14/195 ,  C12N9/93

    摘要： The present invention is to provide an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. A peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II): (I) The mutant protein having the amino acid sequence containing one or more mutations of the above mutations 1 to 38 in the amino acid sequence described in SEQ ID NO:2; and (II) The mutant protein having the amino acid sequence containing one or more mutations selected from the group consisting of substitution, deletion, insertion, addition and inversion at positions other than one or more mutation positions of the above mutation 1 to 38 in the mutant protein described in the above (I), and having a peptide-synthesizing activity.

    摘要翻译： 本发明提供优异的肽合成蛋白质和有效生产肽的方法。 在下列（I）和（II）所示的至少一种蛋白质的存在下，使胺组分和羧基组分反应合成肽：（I）具有含有一个或多个突变的氨基酸序列的突变蛋白 在SEQ ID NO：2中描述的氨基酸序列中的上述突变1至38; 和（II）具有在上述变异株1〜38中的一个以上突变位置以外的位置上具有选自取代，缺失，插入，添加和倒位的一个或多个突变的氨基酸序列的突变蛋白质 上述（I）所述的突变型蛋白质，具有肽合成活性。

    Novel peptide-forming enzyme, microbe producing the enzyme and method for producing peptide using them
    5.
    发明申请
    Novel peptide-forming enzyme, microbe producing the enzyme and method for producing peptide using them 审中-公开
    新型肽形成酶，生产酶的微生物和使用它们生产肽的方法

    公开(公告)号：US20050032187A1

    公开(公告)日：2005-02-10

    申请号：US10775178

    申请日：2004-02-11

    申请人： Kenzo Yokozeki ,  Sonoko Suzuki ,  Seiichi Hara ,  Satoshi Katayama

    发明人： Kenzo Yokozeki ,  Sonoko Suzuki ,  Seiichi Hara ,  Satoshi Katayama

    IPC分类号： C12N1/21 ,  C12N9/00 ,  C12N9/52 ,  C12P21/02 ,  C12P21/06 ,  C07H21/04

    CPC分类号： C12N9/93 ,  C12N9/52 ,  C12P21/02 ,  C12R1/01

    摘要： The present invention relates to a novel enzyme that allows peptide to be produced easily, inexpensively and at high yield without going through a complex synthesis method. More particularly, the present invention provides a novel enzyme that catalyzes a peptide-producing reaction from a carboxy component and an amine component, a microbe that produces the enzyme, and a method for inexpensive production of peptides using this enzyme or microbe. The novel enzyme that efficiently produces peptide was discovered from a newly discovered microbe belonging to the genus Empedobacter, and a method was found that allows peptides to be produced inexpensively and easily.

    摘要翻译： 本发明涉及一种能够容易地，低成本地以高产率生产肽而不经过复合合成方法的新型酶。 更具体地说，本发明提供了一种从羧基组分和胺组分催化产生肽的反应的新型酶，产生酶的微生物，以及使用该酶或微生物廉价生产肽的方法。 从新发现的属于Empedobacter属的微生物发现有效产生肽的新型酶，并且发现能够廉价且容易地生产肽的方法。

    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase
    8.
    发明申请
    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase 审中-公开
    二肽生产方法，其中使用的L-氨基酸酰胺水解酶，以及L-氨基酸酰胺水解酶的制备方法

    公开(公告)号：US20070042459A1

    公开(公告)日：2007-02-22

    申请号：US11249429

    申请日：2005-10-14

    申请人： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    发明人： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    IPC分类号： C12P21/06 ,  C12N9/48

    CPC分类号： C12P21/02 ,  C07K5/06026 ,  C12N9/48

    摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.

    摘要翻译： 提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。 通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或 来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物 。 该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。

    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase
    9.
    发明授权
    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase 失效
    二肽生产方法，其中使用的L-氨基酸酰胺水解酶，以及L-氨基酸酰胺水解酶的制备方法

    公开(公告)号：US07037673B2

    公开(公告)日：2006-05-02

    申请号：US10481087

    申请日：2002-07-26

    申请人： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    发明人： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    IPC分类号： C12P21/00 ,  C12N9/58

    CPC分类号： C12P21/02 ,  C07K5/06026 ,  C12N9/48

    摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.

    摘要翻译： 提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。 通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或 来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物 。 该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。

    Mutant microorganism and method for producing peptide using the same
    10.
    发明申请
    Mutant microorganism and method for producing peptide using the same 审中-公开
    突变体微生物及其使用方法

    公开(公告)号：US20050176150A1

    公开(公告)日：2005-08-11

    申请号：US11050829

    申请日：2005-02-07

    申请人： Ikuo Kira ,  Kenzo Yokozeki ,  Sonoko Suzuki ,  Yasuhiro Mihara ,  Yoshinori Hirao

    发明人： Ikuo Kira ,  Kenzo Yokozeki ,  Sonoko Suzuki ,  Yasuhiro Mihara ,  Yoshinori Hirao

    IPC分类号： C12N1/21 ,  C12N9/88 ,  C12N15/74

    CPC分类号： C12N9/88 ,  C12N15/74

    摘要： The present invention provides a method for producing the peptides comprising: cultivating in medium the microorganisms wherein at least one gene selected from the group consisting of a gene encoding; aminoacylhistidine peptidase; a gene encoding leucylaminopeptidase; and a gene encoding isoaspartyldipeptidase, respectively; has been disrupted on the chromosome and wherein preferably transformed with the recombinant DNA, comprising polynucleotide encoding the proteins having peptide-synthesizing activity, mixing at least one of the cultivated microorganisms and the disrupted cells of the microorganisms with the carboxy and amine components for the peptide synthesis.

    摘要翻译： 本发明提供了一种生产肽的方法，包括：在培养基中培养微生物，其中至少一种选自下组的基因：基因编码; 氨酰基组氨酸肽酶; 编码亮氨酰氨基肽酶的基因; 和分别编码异天竺葵酰二肽酶的基因; 已经在染色体上被破坏，并且其中优选用重组DNA转化，包括编码具有肽合成活性的蛋白质的多核苷酸，将至少一种培养的微生物和微生物的破坏细胞与肽的羧基和胺组分混合 合成。