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公开(公告)号:US20070190602A1
公开(公告)日:2007-08-16
申请号:US11316926
申请日:2005-12-27
申请人: Isao Abe , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
发明人: Isao Abe , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
CPC分类号: C12N9/93
摘要: The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II).(I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2.(II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208
摘要翻译: 本发明的目的在于提供优异的肽合成蛋白质和有效生产肽的方法。 在下列(I)和(II)所示的至少一种蛋白质存在下,使胺组分和羧基组分反应合成肽。 (I)具有包含SEQ ID NO:2的氨基酸序列中的任何突变1至68和突变239至290和324至377的一个或多个突变的氨基酸序列的突变蛋白。 (II)具有包含SEQ ID NO:208的氨基酸序列中的任何突变L1至L335和M1至M642的一个或多个突变的氨基酸序列的突变蛋白
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公开(公告)号:US20070292916A1
公开(公告)日:2007-12-20
申请号:US11765926
申请日:2007-06-20
申请人: Isao ABE , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
发明人: Isao ABE , Rie Takeshita , Seiichi Hara , Sonoko Suzuki , Kenzo Yokozeki , Masakazu Sugiyama , Shunichi Suzuki , Kunihiko Watanabe , Nobuhisa Shimba , Takefumi Nakamura , Uno Tagami , Yuya Kodama , Hiromi Onoye , Reiko Yuuji , Eiichiro Suzuki , Tatsuki Kashiwagi , Ningchun Xu , Yuko Kai
CPC分类号: C07K14/00 , C07K14/195 , C12N9/93
摘要: The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II). (I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2. (II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208
摘要翻译: 本发明的目的在于提供优异的肽合成蛋白质和有效生产肽的方法。 在下列(I)和(II)所示的至少一种蛋白质存在下,使胺组分和羧基组分反应合成肽。 (I)具有包含SEQ ID NO:2的氨基酸序列中的任何突变1至68和突变239至290和324至377的一个或多个突变的氨基酸序列的突变蛋白。 (II)具有包含SEQ ID NO:208的氨基酸序列中的任何突变L1至L335和M1至M642的一个或多个突变的氨基酸序列的突变蛋白
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公开(公告)号:US20060188976A1
公开(公告)日:2006-08-24
申请号:US11311467
申请日:2005-12-20
申请人: Rie Takeshita , Isao Abe , Masakazu Sugiyama , Kenzo Yokozeki , Seiichi Hara , Sonoko Suzuki , Shunichi Suzuki , Kunihiko Watanabe
发明人: Rie Takeshita , Isao Abe , Masakazu Sugiyama , Kenzo Yokozeki , Seiichi Hara , Sonoko Suzuki , Shunichi Suzuki , Kunihiko Watanabe
CPC分类号: C07K14/00 , C07K14/195 , C12N9/93
摘要: The present invention is to provide an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. A peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II): (I) The mutant protein having the amino acid sequence containing one or more mutations of the above mutations 1 to 38 in the amino acid sequence described in SEQ ID NO:2; and (II) The mutant protein having the amino acid sequence containing one or more mutations selected from the group consisting of substitution, deletion, insertion, addition and inversion at positions other than one or more mutation positions of the above mutation 1 to 38 in the mutant protein described in the above (I), and having a peptide-synthesizing activity.
摘要翻译: 本发明提供优异的肽合成蛋白质和有效生产肽的方法。 在下列(I)和(II)所示的至少一种蛋白质的存在下,使胺组分和羧基组分反应合成肽:(I)具有含有一个或多个突变的氨基酸序列的突变蛋白 在SEQ ID NO:2中描述的氨基酸序列中的上述突变1至38; 和(II)具有在上述变异株1〜38中的一个以上突变位置以外的位置上具有选自取代,缺失,插入,添加和倒位的一个或多个突变的氨基酸序列的突变蛋白质 上述(I)所述的突变型蛋白质,具有肽合成活性。
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4.
公开(公告)号:US20110081678A1
公开(公告)日:2011-04-07
申请号:US12943509
申请日:2010-11-10
CPC分类号: C12P21/02 , C07K5/0202 , C12N9/48
摘要: The present invention provides a method for efficiently producing a β-alanyl-amino acid (e.g., carnosine) or derivative thereof. Specifically, it provides a method for producing a β-alanyl-amino acid (e.g., β-alanyl-histidine) or derivative thereof, by reacting a β-alanyl ester or a β-alanyl amide and an amino acid or derivative thereof in the presence of an enzyme or an enzyme-containing product that has an ability to form the β-alanyl-amino acid (e.g., β-alanyl-histidine) or derivative thereof from the β-alanyl ester or the β-alanyl amide and the amino acid (e.g., histidine) or derivative thereof. The present invention also provides a protein which is able to catalyze production of a β-alanyl-amino acid or derivative thereof from a β-alanyl ester or a β-alanyl amide and an amino acid or derivative thereof, and a polynucleotide encoding said protein.
摘要翻译: 本发明提供了一种有效生产β-丙氨酰 - 氨基酸(如肌肽)或其衍生物的方法。 具体地说,本发明提供了一种通过使β-丙氨酰基酯或一种 - 丙氨酰基酰胺与氨基酸或氨基酸反应生产β-丙氨酰 - 氨基酸(例如, - 丙氨酰 - 组氨酸)或其衍生物的方法 其衍生物在酶或含酶产物的存在下具有从丙氨酰酯形成β-淀粉氨基酸(例如,β-丙氨酰 - 组氨酸)或其衍生物的能力,或 丙氨酰酰胺和氨基酸(如组氨酸)或其衍生物。 本发明还提供一种蛋白质,其能够催化β-氨基丙氨酰 - 氨基酸或其衍生物从β-丙氨酰酯或其 - 氨基酸或其衍生物及其衍生物的产生,以及多核苷酸 编码所述蛋白质。
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