公开(公告)号：US20110129938A1

公开(公告)日：2011-06-02

申请号：US13054940

申请日：2009-07-15

申请人： Yuka Kobayashi ,  Jun Hirabayashi ,  Hiroaki Tateno ,  Hirokazu Kawagishi ,  Hideo Dohra

发明人： Yuka Kobayashi ,  Jun Hirabayashi ,  Hiroaki Tateno ,  Hirokazu Kawagishi ,  Hideo Dohra

IPC分类号： G01N33/50 ,  C07K14/37 ,  C07K14/375

CPC分类号： G01N33/574 ,  C07K14/37 ,  C12P21/02 ,  G01N2333/4724 ,  Y10T436/143333

摘要： Disclosed is a novel lectin which can bind specifically to an L-fucose α1→6 sugar chain. Also disclosed is use of the lectin. The L-fucose α1→6 specific lectin of the present invention is characterized in that: (1) the lectin is extracted from a basidiomycete or an ascomycete; (2) the lectin has a molecular weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of 4,000 to 40,000; and (3) the lectin has an affinity to an L-fucose α1→6 sugar chain, the affinity being represented by an association constant of 1.0×104M−1 or more (at 25 degrees C.). The lectin can be used for detecting an L-fucose α1→6 sugar chain specifically, and is effective for the purification of an L-fucose α1→6 sugar chain or a non L-fucose α1→6 sugar chain.

摘要翻译： 公开了一种能够特异性结合L-岩藻糖α1→6糖链的新型凝集素。 还公开了使用凝集素。 本发明的L-岩藻糖α1→6特异性凝集素的特征在于：（1）从担子菌或子囊菌中提取凝集素; （2）凝集素通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS-PAGE）的分子量为4000〜40,000; 和（3）凝集素对L-岩藻糖α1→6糖链具有亲和性，亲和力由1.0×10 4 M -1以上的相关常数（25℃）表示。 凝集素可用于特异性检测L-岩藻糖α1→6糖链，对L-岩藻糖α1→6糖链或非L-岩藻糖α1→6糖链的纯化有效。

公开(公告)号：US20100120146A1

公开(公告)日：2010-05-13

申请号：US12295814

申请日：2007-04-04

申请人： Hideyuki Okano ,  Kazunobu Sawamoto ,  Masanori Sakaguchi ,  Jun Hirabayashi

发明人： Hideyuki Okano ,  Kazunobu Sawamoto ,  Masanori Sakaguchi ,  Jun Hirabayashi

IPC分类号： C12N5/02

CPC分类号： C12N5/0623

摘要： The present invention is directed to providing the activator of the integrin signaling in neural stem cells or neural progenitors and the method for using the same. The activator of the integrin signaling comprises galectin-1 and can be used as a regulator that regulates maintenance, survival, or differentiation of a neural stem cells or a neural progenitor.

摘要翻译： 本发明涉及在神经干细胞或神经祖细胞中提供整联蛋白信号传导的激活剂及其使用方法。 整联蛋白信号传导的激活剂包含半乳凝素-1，可用作调节神经干细胞或神经祖细胞的维持，存活或分化的调节剂。

公开(公告)号：US20070269833A1

公开(公告)日：2007-11-22

申请号：US11751106

申请日：2007-05-21

申请人： Toshikazu Minamisawa ,  Kiyoshi Suzuki ,  Jun Hirabayashi

发明人： Toshikazu Minamisawa ,  Kiyoshi Suzuki ,  Jun Hirabayashi

IPC分类号： A61K38/17 ,  G01N33/53 ,  C07K14/47

CPC分类号： G01N33/53 ,  A61K38/1703 ,  G01N2400/40

摘要： A galectin-glycosaminoglycan complex and a method for controlling galectin activity.

摘要翻译： 半乳凝素 - 糖胺聚糖复合物和控制半乳凝素活性的方法。

公开(公告)号：US20070202535A1

公开(公告)日：2007-08-30

申请号：US10596692

申请日：2004-12-24

申请人： Jun Hirabayashi ,  Atsushi Kuno ,  Sachiko Nakamura ,  Noboru Uchiyama

发明人： Jun Hirabayashi ,  Atsushi Kuno ,  Sachiko Nakamura ,  Noboru Uchiyama

IPC分类号： G01N33/53 ,  C07K14/705

CPC分类号： G01N33/54393 ,  G01N33/543 ,  Y10S435/97

摘要： As a result of investigating the optimum conditions of methods for immobilizing proteins that interact with sugar chains onto a substrate, it was revealed that coating the surface of a slide glass with GTMS enables immobilization at a higher S/N ratio than conventionally possible. Moreover, by using a substrate to which a rubber with a number of holes was affixed to form a number of reaction vessels, and further by spotting lectins onto the substrate and washing with PBST, the weak interactions between sugar chains and lectins were successfully detected with improved sensitivity. In addition, by introducing an evanescent excitation-type scanner, it became possible to detect the interactions between lectins and sugar chains without washing away the probe solution.

摘要翻译： 通过研究将与糖链相互作用的蛋白质固定在基质上的最佳条件的结果，显示用GTMS涂覆载玻片的表面使得能够以比传统可能的更高的S / N比固定。 此外，通过使用固定有多个孔的橡胶的基板以形成多个反应容器，并且进一步通过将凝集素点样到基板上并用PBST洗涤，成功检测到糖链和凝集素之间的弱相互作用 提高灵敏度。 此外，通过引入ev逝激发型扫描仪，可以检测凝集素和糖链之间的相互作用而不洗掉探针溶液。

公开(公告)号：US20060154303A1

公开(公告)日：2006-07-13

申请号：US11331959

申请日：2006-01-13

申请人： Toru Myogadani ,  Youji Ebe ,  Masao Yamada ,  Jun Hirabayashi

发明人： Toru Myogadani ,  Youji Ebe ,  Masao Yamada ,  Jun Hirabayashi

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/54373 ,  G01N21/6452 ,  G01N21/648 ,  G01N33/582

摘要： In the case where many interactional substances are immobilized on the front surface side of a light transmitting plate capable of generating evanescent waves on the front surface, and where the fluorescence levels in the interactions between the interactional substances and a fluorescence labeled test substance are detected in a liquid layer according to a conventional method, the detection sensitivity and S/N ratio are low due to the background noise. Furthermore, it is necessary to perform a gel filtration purification step for the purpose of removing an excessive amount of the fluorescence dye used for labeling a test substance before the interactions between the fluorescence labeled test substance and the interactional substances are performed. To solve these problems, this invention proposes the above-mentioned method for detecting the evanescent wave excited fluorescence comprising the step of adding a black colorant, for example, a pigment based colorant mainly containing fine particles such as Bokuju.

摘要翻译： 在许多相互作用物质被固定在能够在前表面上产生ev逝波的透光板的前表面侧，并且其中在相互作用的物质和荧光标记的测试物质之间的相互作用中的荧光水平被检测到的情况下 根据常规方法的液体层，由于背景噪声，检测灵敏度和S / N比都较低。 此外，为了在进行荧光标记的测试物质和相互作用物质之间的相互作用之前去除用于标记测试物质的过量的荧光染料，需要进行凝胶过滤纯化步骤。 为了解决这些问题，本发明提出了上述用于检测ev逝波激发荧光的方法，其包括添加黑色着色剂的步骤，例如主要含有诸如Bokuju的微粒的颜料基着色剂。

公开(公告)号：US06839530B2

公开(公告)日：2005-01-04

申请号：US10356583

申请日：2003-02-03

申请人： Norio Takahashi ,  Jun Hirabayashi

发明人： Norio Takahashi ,  Jun Hirabayashi

IPC分类号： G03G15/02 ,  G03G15/08 ,  G03G21/00 ,  G03G15/30

CPC分类号： G03G15/0216

摘要： An image forming apparatus includes an image bearing member; a charging roller cooperable with the image bearing member to form a nip therebetween, for charging the charging roller is rotatable for counterdirectional peripheral movement relative to the image bearing member at the nip; a developing unit for developing with a developer an electrostatic image formed on the image bearing member; wherein the potential of a potential changing portion of the image bearing member changes with respect to the peripheral moving direction of the image bearing member; and wherein at tA (sec) after arrival at an upstream end of the nip, of the potential changing portion, the developing unit is supplied with a voltage for collecting particles from the image bearing member to the developing unit, where tA (sec) is a sum of a time duration required for a surface of the charging roller to move from the upstream end of the nip to a downstream end of the nip and a time duration required for a surface of the image bearing member to move from the downstream end of the nip to a developing zone of the developing unit.

公开(公告)号：US06614460B2

公开(公告)日：2003-09-02

申请号：US09991711

申请日：2001-11-26

申请人： Masato Susukida ,  Katsuto Nagano ,  Yoshio Saita ,  Jun Hirabayashi ,  Jun Hagiwara

发明人： Masato Susukida ,  Katsuto Nagano ,  Yoshio Saita ,  Jun Hirabayashi ,  Jun Hagiwara

IPC分类号： B41J2335

CPC分类号： B41J2/3353 ,  B41J2/3357 ,  B41J2/3358

摘要： A thermal head including a protection layer having mutually opposed first and second surfaces, said first surface having a flat or protruded printing surface which is brought into contact with a heat sensitive record medium, a heat generating section including resistors and electrodes connected to the electrodes and provided on said second surface of the protection layer, and a reinforcing member made of a low melting pint glass and provided on a side of the heat generating section remote from the protection layer. The reinforcing member improves a mechanical strength of the thermal head. The reinforcing member made of a glass also serves as a heat storage member, and thus a thermal property of the thermal head is improved. The reinforcing member may be formed by an aggregate of ceramic particles. The reinforcing member may contain a heat storage layer made of a low melting point glass and a heat conduction layer provided on the heat storage layer.