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    METHODS FOR NUCLEAR REPROGRAMMING OF CELLS
    73.
    发明申请
    METHODS FOR NUCLEAR REPROGRAMMING OF CELLS 审中-公开
    细胞核代谢的方法

    公开(公告)号：US20150086649A1

    公开(公告)日：2015-03-26

    申请号：US14493107

    申请日：2014-09-22

    申请人： LONZA LTD

    发明人： PATRICK WALSH ,  THOMAS FELLNER

    IPC分类号： C12N5/074 ,  A61K33/08

    摘要： Described herein are methods for enhancing the nuclear reprogramming of somatic cells to become induced pluripotent stem cells. In particular, the methods disclosed herein involve the use of damage-associated molecular pattern molecules (DAMP). In certain embodiments the DAMPs are aluminum compositions such as aluminum hydroxide. Such DAMPs have unexpectedly and surprisingly been found to enhance the nuclear reprogramming efficiency of the reprogramming factors commonly used to induce somatic cells to become induced pluripotent stem cells. Accordingly, this disclosure describes methods of nuclear reprogramming as well as cells obtained from such methods along with therapeutic methods for using such cells for the treatment of disease amendable to treatment by stem cell therapy; as well as kits for such uses.

    摘要翻译： 本文描述了用于增强体细胞核重编程以成为诱导多能干细胞的方法。 特别地，本文公开的方法涉及使用损伤相关分子模式分子（DAMP）。 在某些实施方案中，DAMP是铝组合物，例如氢氧化铝。 已经意外地惊奇地发现了这种DAMP以提高通常用于诱导体细胞成为诱导多能干细胞的重编程因子的核重编程效率。 因此，本公开描述了核重新编程的方法以及从这些方法获得的细胞以及使用这种细胞治疗可通过干细胞治疗治疗的疾病的治疗方法; 以及用于此类用途的套件。

    REGULATABLE PROMOTER
    77.
    发明申请
    REGULATABLE PROMOTER 有权
    可调节的促销者

    公开(公告)号：US20140242636A1

    公开(公告)日：2014-08-28

    申请号：US14350352

    申请日：2012-10-05

    申请人： LONZA LTD.

    发明人： Diethard Mattanovich ,  Brigitte Gasser ,  Michael Maurer ,  Roland Prielhofer ,  Joachim Klein ,  Jana Wenger

    IPC分类号： C12N15/63 ,  C07K16/00 ,  C12P21/00

    CPC分类号： C12N15/815 ,  C07K14/39 ,  C07K16/00 ,  C07K2317/55 ,  C12N9/0008 ,  C12N15/635 ,  C12N15/67 ,  C12N15/81 ,  C12P21/00 ,  C12Y102/01012

    摘要： A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.

    摘要翻译： 一种通过培养包含表达构建体的重组真核生物细胞系（包括可调节启动子和编码所述启动子的转录控制下的POI的核酸分子）的重组真核生物细胞系的方法，包括以下步骤：a）培养细胞 与抑制启动子的基础碳源连接，b）用限制量的补充碳源培养细胞系，抑制启动子以与天然存在相比以至少15％的转录速率诱导POI的产生 pGAP启动子，和c）生产和回收POI; 并进一步分离可调节的启动子和相应的表达系统。

    Customizable facility
    78.
    发明授权
    Customizable facility 有权

    公开(公告)号：US11613900B2

    公开(公告)日：2023-03-28

    申请号：US17553283

    申请日：2021-12-16

    申请人： LONZA LTD

    发明人： Jonathan Fortin ,  Lesley Wood ,  Stefan Widmer ,  Sandra Carbonneau ,  Alexander Zahiri

    IPC分类号： E04H1/00 ,  F24F3/167 ,  C12M1/12 ,  E04H5/02 ,  E04H5/00 ,  E04B1/00

    摘要： A customizable facility for manufacturing at least one product has at least one modular unit in communication with at least one central unit configured to provide utilities to the at least one modular unit. In some embodiments, the central unit(s) and modular unit(s) are positioned at least partially within a shell. Each modular unit can be a fermentation unit, a pre-viral unit, a post-viral unit, a utility space, a warehouse, a media buffer facility, an office, a personnel unit, or another unit. The modular units can be arranged to maximize a number of modular units within the shell while minimizing a footprint of the shell. A method of assembling a facility for manufacturing at least one product includes providing at least one central unit and providing at least one modular unit in communication with the central unit(s) such that utilities are provided by the central unit(s) to the modular unit(s).

    Single Use Bioreactor
    80.
    发明申请
    Single Use Bioreactor 有权

    公开(公告)号：US20230045226A1

    公开(公告)日：2023-02-09

    申请号：US17850452

    申请日：2022-06-27

    申请人： Lonza Ltd

    发明人： Colin Mark Jaques ,  Mohsan Waseem Khan ,  Rita D'Ornelas P. De Barros Costa ,  Anthony Beaney ,  David Valentine

    IPC分类号： C12M1/00 ,  C12M1/06 ,  B01F23/231 ,  B01F27/053 ,  B01F27/86 ,  B01F27/191 ,  B01F27/113 ,  B01F35/513

    摘要： A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.