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173 条记录 (耗时 0.025 秒)

    Method for introduction of an exogenous genetic substance or a physiologically active compound
    74.
    发明授权
    Method for introduction of an exogenous genetic substance or a physiologically active compound 有权
    引入外源遗传物质或生理活性化合物的方法

    公开(公告)号：US06596540B2

    公开(公告)日：2003-07-22

    申请号：US09956166

    申请日：2001-09-20

    申请人： Kiichi Fukui ,  Akio Kobayashi ,  Satoshi Harashima ,  Eiichiro Fukusaki ,  Takefumi Sone

    发明人： Kiichi Fukui ,  Akio Kobayashi ,  Satoshi Harashima ,  Eiichiro Fukusaki ,  Takefumi Sone

    IPC分类号： C12N1500

    CPC分类号： C12N15/89

    摘要： A novel method for introduction of an exogenous substance or a physiologically active compound into cells is provided according to this invention. This method can realize introduction of an exogenous genetic substance or a physiologically active compound of large size with a large amount. Such substance is immobilized to beads of sphere fine particles having a particle size of 0.01 mm to 10 mm, and bio-active beads thus produced are introduced into cells. Bio-active beads comprising calcium alginate are particularly useful for the purpose of the present invention.

    摘要翻译： 根据本发明提供了将外源物质或生理活性化合物引入细胞的新方法。 该方法可以实现大量外源遗传物质或生理活性化合物的引入。 将该物质固定在粒径为0.01mm〜10mm的球状微粒子珠上，将生成的生物活性珠子导入到细胞中。 包含藻酸钙的生物活性珠特别适用于本发明的目的。

    Method to promote growth of a plant
    75.
    发明授权
    Method to promote growth of a plant 失效
    促进植物生长的方法

    公开(公告)号：US06465396B1

    公开(公告)日：2002-10-15

    申请号：US09517427

    申请日：2000-03-02

    申请人： Akio Kobayashi ,  Eiichiro Fukusaki ,  Akira Isogai

    发明人： Akio Kobayashi ,  Eiichiro Fukusaki ,  Akira Isogai

    IPC分类号： A01N3100

    CPC分类号： A01N31/02 ,  A01N37/02

    摘要： A concise method to promote growth of a plant useful for increase of food production. This invention provides a method to promote growth of a plant by administration of formic acid, an organic acid with a low molecular weight through avoidance of photo-oxidation damage. This invention also provides amino acid sequence of formate dehydrogenase and base sequence encoding the enzyme. The expression of the enzyme is induced by administration of formic acid and the enzyme is involved in metabolism of formic acid and its salts. Furthermore, this invention provides a method to promote growth of a plant by administration of methanol through avoidance of photo-oxidation damage.

    摘要翻译： 促进有助于增加粮食生产的植物生长的简明方法。 本发明提供一种方法，通过避免光氧化损伤，通过施用甲酸，低分子量的有机酸来促进植物的生长。 本发明还提供了编码该酶的甲酸脱氢酶和碱基序列的氨基酸序列。 通过施用甲酸诱导酶的表达，酶参与甲酸及其盐的代谢。 此外，本发明提供了一种通过避免光氧化损伤来施用甲醇促进植物生长的方法。

    Method of manufacturing spline shaft
    77.
    发明授权
    Method of manufacturing spline shaft 失效
    花键轴的制造方法

    公开(公告)号：US06336351B1

    公开(公告)日：2002-01-08

    申请号：US08842990

    申请日：1996-12-20

    申请人： Akio Kobayashi ,  Katsuyuki Morozumi

    发明人： Akio Kobayashi ,  Katsuyuki Morozumi

    IPC分类号： B21K130

    CPC分类号： B21K1/10 ,  B21C37/30 ,  B21K1/063 ,  B21K1/066 ,  B21K3/00

    摘要： A method of manufacturing a spline shaft, comprising ironing a hollow stock by cold forging and forming splines in a desired portion of said hollow stock by cold forging by using its work harden state. This method makes it possible to reduce the weight of a product by using a hollow stock, and to prevent buckling of the material of the hollow stock during the formation of splines owing to work-hardening caused by the ironing. Further, a predetermined root strength can be ensured without requiring induction hardening. Accordingly, a reduction in weight is achieved, and the production speed and yield are improved.

    摘要翻译： 一种花键轴的制造方法，其特征在于，包括通过冷锻而使中空坯料进行熨烫，并通过使用其加工硬化状态进行冷锻而在所述中空坯料的所需部分形成花键。 该方法使得可以通过使用中空坯料来减小产品的重量，并且防止由于熨烫引起的加工硬化而在形成花键期间中空坯料的屈曲。 此外，可以确保预定的根强度，而不需要感应淬火。 因此，实现了重量的减轻，并且提高了生产速度和产率。

    Motion vector detection circuit and object tracking camera device utilizing the same
    78.
    发明授权
    Motion vector detection circuit and object tracking camera device utilizing the same 失效
    运动矢量检测电路和物体跟踪摄像装置

    公开(公告)号：US6002428A

    公开(公告)日：1999-12-14

    申请号：US546219

    申请日：1995-10-20

    申请人： Hideki Matsumura ,  Akio Kobayashi ,  Hiroshi Nakashima

    发明人： Hideki Matsumura ,  Akio Kobayashi ,  Hiroshi Nakashima

    IPC分类号： G01S3/786 ,  H04N5/14 ,  H04N7/18 ,  H04N5/32

    CPC分类号： G01S3/7864 ,  H04N5/145 ,  H04N7/188

    摘要： A motion vector detection circuit includes a registration determining switch which sets a first registration determination mode wherein at an initial field, correlation values are evaluated by a correlator on the basis of integrated color data of a specific detection block being stored in a representative color memory and integrated color data of twenty-five (25) detection blocks being stored in an integrated color memory. A comparison circuit compares the correlation values with a predetermined threshold value, and if a condition that the threshold value is larger than the correlation value is satisfied in each of eighty (80) percents or more detection blocks, the integrated color data stored in the representative color memory is registered as a representative color of an object, and otherwise, no registration is performed. In a second registration determination mode set by the registration determining switch, the correlator evaluates correlation values on the basis of the integrated color data of the specific detection block and integrated color data of four (4) detection block.

    摘要翻译： 一种运动矢量检测电路，包括：登记确定开关，其设置第一登记确定模式，其中在初始场中，相关器根据存储在代表性颜色存储器中的特定检测块的积分颜色数据由相关器评估相关值;以及 存储在集成彩色存储器中的二十五（25）个检测块的综合颜色数据。 比较电路将相关值与预定阈值进行比较，并且如果在八十（80）个百分比以上的检测块中满足阈值大于相关值的条件，则存储在代表中的积分色数据 颜色存储器被注册为对象的代表颜色，否则不执行注册。 在由注册确定开关设置的第二注册确定模式中，相关器基于特定检测块的积分颜色数据和四（4）个检测块的积分颜色数据来评估相关值。

    Method of forming internal spline shaft
    79.
    发明授权
    Method of forming internal spline shaft 失效
    形成内花键轴的方法

    公开(公告)号：US5730018A

    公开(公告)日：1998-03-24

    申请号：US781269

    申请日：1997-01-10

    申请人： Akio Kobayashi ,  Katsuyuki Morozumi

    发明人： Akio Kobayashi ,  Katsuyuki Morozumi

    IPC分类号： F16C3/02 ,  B21C37/20 ,  B21K1/12 ,  B21K1/30

    CPC分类号： B21C37/202 ,  B21K1/063 ,  B21K1/066 ,  B21K1/30 ,  Y10T29/49474

    摘要： A method of forming an internal spline shaft, comprising forming splines on an inner circumferential portion of a hollow stock by cold forging in such a way that a clearance "a" between an inner diameter of the hollow stock and an outer diameter of a tool guide portion, a difference "b" between a reduced diameter of a forming land and the inner diameter of the hollow stock, and a forming-land approach angle .theta. satisfy respectively specific relationships. By this method, products having internal long splines can be obtained in a high yield by cold forging method.

    摘要翻译： 一种形成内部花键轴的方法，包括通过冷锻成形在中空坯料的内圆周部分上形成花键，使得中空坯料的内径和工具导向件的外径之间的间隙“a” 成形台面的缩小直径与中空坯料的内径之间的差“b”以及成形面接近角θ分别满足特定的关系。 通过该方法，可以通过冷锻法以高产率获得具有内部长花键的产品。