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77 条记录 (耗时 0.027 秒)

    Chemiluminescence enhancement of enzyme-activated decomposition of enzymatically cleavable chemiluminescent 1,2-dioxetanes
    74.
    发明授权
    Chemiluminescence enhancement of enzyme-activated decomposition of enzymatically cleavable chemiluminescent 1,2-dioxetanes 失效
    酶促活化分解的可溶性CHEMILUMINESCENT 1,2-二氧化物的显色增强

    公开(公告)号：US5145772A

    公开(公告)日：1992-09-08

    申请号：US767566

    申请日：1991-09-30

    申请人： John C. Voyta ,  Brooks Edwards ,  Irena Y. Bronstein ,  Patricia McGrath

    发明人： John C. Voyta ,  Brooks Edwards ,  Irena Y. Bronstein ,  Patricia McGrath

    IPC分类号： C07C43/196 ,  C07C69/00 ,  C07D321/00 ,  C07F9/12 ,  C07F9/655 ,  C07F9/6574 ,  C07H15/203 ,  C07H15/26 ,  C12Q1/34 ,  G01N21/76 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/76

    CPC分类号： C07H15/203 ,  C07C43/196 ,  C07C69/00 ,  C07D321/00 ,  C07F9/12 ,  C07F9/65512 ,  C07F9/65742 ,  C07H15/26 ,  C12Q1/34 ,  G01N21/76 ,  G01N33/533 ,  G01N33/535 ,  G01N33/581 ,  G01N33/582 ,  G01N33/76 ,  C07C2103/00 ,  C07C2103/74 ,  Y10S435/96 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/827 ,  Y10T436/144444

    摘要： Water soluble naturally-occurring and synthetic enhancer substances, generally macromolecular in nature, for example globular proteins that include hydrophobic regions such as bovine serum albumin, and polymeric quaternary ammonium salts such as poly(vinylbenzyltrimethylammonium chloride), which have the ability to inhibit light-emitting fluorophores resulting from the decomposition of chemiluminescent compounds from releasing energy through non-light emitting pathways, are disclosed as permitting the stabilization, and hence increasing the light intensity, of such light-emitting fluorophores in aqueous media as compared to the intensity of the light emitted by the same quantities of such fluorophores in aqueous media in the absence of such enhancer substances. Any chemiluminescent enzymatically cleavable 1,2-dioxetane, for example 3-(2'-spiroadamantane)-4-methoxy-(3"-phosphoryloxy)phenyl-1,2-dioxetane disodium salt, can be used. Auxiliary fluorophores, for example fluorescein and derivatized fluoresceins, that accept energy from fluorophores produced by decomposition of a chemiluminescent compound and in turn emit detectable energy, can also be present. Such enhancer substance/chemiluminescent compound compositions are useful in detecting the presence or determining the concentration of chemical or biological substances in immunoassays, chemical assays and nucleic acid probe assays, and in chemical/physical probe procedures for studying the microstructures of macromolecules.

    摘要翻译： 水溶性天然存在和合成的增强剂物质（通常是大分子的），例如包括疏水区域的牛皮蛋白，例如牛血清白蛋白，以及聚合季铵盐，例如聚（乙烯基苄基三甲基氯化铵） 公开了从化学发光化合物分解而产生的荧光团从释放能量通过非发光途径发射的荧光团，因为与光强度相比，这种发光荧光团在水介质中的稳定性以及因此增加了光强度 在没有这种增强剂物质的情况下，通过相同量的这种荧光团在水性介质中发射。 可以使用任何化学发光的可酶切割的1,2-二氧环乙烷，例如3-（2'-双金刚烷）-4-甲氧基 - （3“ - 磷酰氧基）苯基-1,2-二氧杂环丁烷二钠盐。 也可以存在辅助荧光团，例如荧光素和衍生化的荧光素，其通过化学发光化合物的分解产生的荧光团接受能量，并进而发射可检测的能量。 这种增强剂物质/化学发光化合物组合物可用于检测免疫测定，化学测定和核酸探针测定中的化学或生物物质的浓度的存在或确定，以及用于研究大分子的微结构的化学/物理探针程序。

    Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes
    75.
    发明授权
    Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes 失效
    化学发光3-（取代的金刚烷-2'-亚基）1,2-二氧杂环丁烷

    公开(公告)号：US5112960A

    公开(公告)日：1992-05-12

    申请号：US574786

    申请日：1990-08-30

    申请人： Irena Y. Bronstein ,  Brooks Edwards ,  Rouh-Rong Juo

    发明人： Irena Y. Bronstein ,  Brooks Edwards ,  Rouh-Rong Juo

    IPC分类号： C07C43/178 ,  C07C43/196 ,  C07C43/215 ,  C07C69/00 ,  C07D321/00 ,  C07F9/12 ,  C07F9/655 ,  C07F9/6574 ,  C07H15/203 ,  C07H15/26 ,  C12Q1/34 ,  G01N21/76 ,  G01N33/58 ,  C07H15/24 ,  C07F9/06

    CPC分类号： C07H15/203 ,  C07C43/1788 ,  C07C43/196 ,  C07C43/215 ,  C07C69/00 ,  C07D321/00 ,  C07F9/12 ,  C07F9/65512 ,  C07F9/65742 ,  C07H15/26 ,  C12Q1/34 ,  G01N33/581 ,  G01N33/582 ,  C07C2103/00 ,  C07C2103/74

    摘要： Enzymatically cleavable chemiluminescent 1,2-dioxetane compounds capable of producing light energy when decomposed, substantially stable at room temperature before a bond by which an enzymatically cleavable labile substituent thereof is intentionally cleaved, are disclosed. These compounds can be represented by the formula: ##STR1## wherein: X and X.sup.1 each represent, individually, hydrogen, a hydroxyl group, a halo substituent, an unsubstituted lower alkyl group, a hydroxy (lower) alkyl group, a halo (lower) alkyl group, a phenyl group, a halophenyl group, an alkoxyphenyl group, a hydroxyalkoxy group, a cyano group or an amide group, with at least one of X and X.sup.1 being other than hydrogen; andR.sub.1 and R.sub.2, individually or together, represent an organic substituent that does not interfere with the production of light when the dioxetane compound is enzymatically cleaved and that satisfies the valence of the dioxetane compound's 4-carbon atom, with the provisos that if R.sub.1 and R.sub.2 represent individual substituents the R.sub.2 substituent is aromatic, heteroaromatic, or an unsaturated substituent in conjugation with an aromatic ring, and that at least one of R.sub.1 and R.sub.2 is, or R.sub.1 and R.sub.2 taken together are, an enzymatically cleavable labile group-substituted fluorescent chromophore group that produces a luminescent substance when the enzymatically cleavable labile substituent thereof is removed by an enzyme.The corresponding dioxetanes which, instead of being substituted at the 5' or 7', or at the 5' and 7' positions, instead contain a 4' methylene group, are also disclosed, as are intermediates for all these 3-substituted adamant-2'-ylidenedioxetanes, and their use as reporter molecules in assays.

    摘要翻译： 公开了能够在分解时产生光能的酶促切割的化学发光的1,2-二氧杂环丁烷化合物，在其中有可能将可酶切割的不稳定取代基故意断裂的键之间在室温下基本稳定。 这些化合物可以由下式表示：其中：X和X1各自独立地表示氢，羟基，卤素取代基，未取代的低级烷基，羟基（低级）烷基，卤代（低级） ）烷基，苯基，卤代苯基，烷氧基苯基，羟基烷氧基，氰基或酰胺基，其中X和X 1中的至少一个不是氢; 并且R 1和R 2各自或一起表示当二氧杂环丁烷化合物被酶切并且满足二恶丁环化合物的4-碳原子的化合价时不干扰光的产生的有机取代基，条件是如果R1和 R2表示各个取代基，R2取代基是芳族的，杂芳族的，或与芳环共轭的不饱和取代基，R1和R2中的至少一个是，或者R1和R2一起是可酶切的不稳定基团取代的荧光 当其酶促切割的不稳定取代基被酶除去时产生发光物质的发色团。 也公开了相对于在5'或7'或5'和7'位置取代含有4'亚甲基的二氧杂环丁烷，以及所有这些3-取代的金刚烷基 - 2'-亚基二氧杂环丁烷，以及它们在测定中作为报道分子的用途。

    Dioxetanes for use in assays
    76.
    发明授权
    Dioxetanes for use in assays 失效
    用于测定的二氧杂环丁烷

    公开(公告)号：US5089630A

    公开(公告)日：1992-02-18

    申请号：US512030

    申请日：1990-04-13

    申请人： Irena Y. Bronstein ,  Brooks Edwards ,  Larry Kricka ,  John Voyta

    发明人： Irena Y. Bronstein ,  Brooks Edwards ,  Larry Kricka ,  John Voyta

    IPC分类号： C07D321/00 ,  C07D491/10 ,  C07D493/10 ,  C07D495/10 ,  C07F9/655 ,  C07F9/6561 ,  C12P17/02 ,  G01N33/533 ,  G01N33/58

    CPC分类号： C07D491/10 ,  C07D321/00 ,  C07D493/10 ,  C07D495/10 ,  C07F9/65512 ,  C07F9/6561 ,  C12P17/02 ,  G01N33/533 ,  G01N33/582

    摘要： A dioxetane that includes a fluorescent chromophore spiro-bound at the 4-carbon of the dioxetane. The dioxetane has the formula ##STR1## where X is CR.sub.7 R.sub.8, O, S, or N-R (where each R.sub.7, R.sub.8, and R, independently, is H, alkyl, heteroalkyl, aryl, heteroaryl, cycloalkyl, cycloheteroalkyl, aralkyl, alkaryl, or an enzyme cleavable group), and each R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, andR.sub.6, independently, is H, an electron-withdrawing group, an electron-donating group, heteroaryl, or an enzyme cleavable group, or groups R.sub.1 -R.sub.6 together form a ring, and T is a substituted or unsubstituted aryl, polyaryl, cycloalkylidene or polycycloalkylidene group spiro-bound at the 3-carbon of the dioxetane.The dioxetane can be decomposed by direct cleavage of the dioxetane 0--0 bond or by cleavage of an enzyme cleavable group bonded to the dioxetane to form a luminescent substance that includes the coumarin portion of the dioxetane.The dioxetanes are used in an assay to detect a member of a specific binding pair or an enzyme.

    摘要翻译： 包含在二氧杂环丁烷的4-碳上螺旋结合的荧光发色团的二氧环乙烷。 二氧杂环丁烷具有下式：其中X是CR 7 R 8，O，S或NR（其中每个R 7，R 8和R独立地是H，烷基，杂烷基，芳基，杂芳基，环烷基，环杂烷基，芳烷基，烷芳基， 或可裂解酶基团），并且每个R 1，R 2，R 3，R 4，R 5和R 6独立地为H，吸电子基团，给电子基团，杂芳基或酶可裂解基团，或基团R1- R6一起形成环，T是取代或未取代的芳基，芳基，亚环烷基或多环亚烷基，其在二氧杂环丁烷的3-碳上螺旋结合。 二氧杂环丁烷可以通过直接切割二氧杂环丁烷0-0键或通过与二氧杂环丁烷键合的酶切割基团切割而分解，形成包含二氧杂环丁烷的香豆素部分的发光物质。 二氧杂环丁烷用于检测特异性结合对或酶的成员的测定中。

    Enzymatically-cleavable chemiluminescent fused polycyclic ring-containing 1,2-dioxetanes
    77.
    发明授权
    Enzymatically-cleavable chemiluminescent fused polycyclic ring-containing 1,2-dioxetanes 失效
    可酶切的化学发光的稠环多环的1,2-二氧杂环丁烷

    公开(公告)号：US4952707A

    公开(公告)日：1990-08-28

    申请号：US213672

    申请日：1988-06-30

    申请人： Brooks Edwards ,  Irena Y. Bronstein ,  Alison A. Laird ,  John C. Voyta

    发明人： Brooks Edwards ,  Irena Y. Bronstein ,  Alison A. Laird ,  John C. Voyta

    IPC分类号： C07C43/215 ,  C07C43/225 ,  C07C43/23 ,  C07C49/84 ,  C07C69/00 ,  C07D321/00 ,  C07D405/04 ,  C07F9/655 ,  C07H17/04 ,  C09K11/06 ,  C09K11/07 ,  C12Q1/34 ,  G01N33/58

    CPC分类号： C07C43/215 ,  C07C43/225 ,  C07C43/23 ,  C07C49/84 ,  C07C69/00 ,  C07D321/00 ,  C07F9/65512 ,  C12Q1/34 ,  G01N33/582 ,  C07C2103/00 ,  C07C2103/74 ,  Y10S435/968 ,  Y10S435/973

    摘要： Chemiluminescent 1,2-dioxetane compounds are disclosed in which the molecule is stabilized at the 3-position on the dioxetane ring against decomposition prior to the molecule's coming in contact with a labile group-removing substance (e.g., an enzyme that will cleave the labile group to cause the molecule to decompose to form at least one light-emitting fluorophore) and substituted at the 4-position on the dioxetane ring with a fused polycyclic ring-containing fluorophore moiety bearing a labile ring substituent whose point of attachment to the fused polycyclic ring, in relation to this ring's point(s) of attachment to the dioxetane ring, is such that the total number of ring atoms separating these points of attachment, including the ring atoms at the points of attachment, is an odd whole number. These odd pattern substituted compounds decompose to emit light of greater intensity and of a different wavelength than that emitted by the corresponding even pattern substituted isomers. They are useful in detecting the presence or determining the concentration of chemical or biological substances in immunoassays, chemical assays and nucleic acid probe assays, and in chemical/physical probe procedures for studying the microstructures of macromolecules. Two or more of them can also be used in combination, or one or more of them can be used together with other chemiluminescent compounds, in multi-channel assays to detect two or more different analytes simultaneously. Novel intermediates used in the preparation of these odd pattern substituted compounds are also disclosed.

    摘要翻译： 公开了化学发光的1,2-二氧杂环丁烷化合物，其中在分子与不稳定基团除去物质（例如，将切割不稳定的酶的酶接触）之前，分子在二氧杂环丁烷环上的3-位上被稳定化，免于分解 基团，使分子形成至少一个发光的荧光团），并用含有稠环多环的荧光团部分在二氧杂环丁烷环上的4位上取代，该部分带有不饱和环取代基，其连接点与稠合多环 环相对于该环的连接到二氧杂环丁烷环的点是使得这些连接点（包括连接点处的环原子）分离的环原子的总数为奇数整数。 这些奇数图案取代的化合物分解发射比由相应的均匀图案取代的异构体发射的光更强的和不同波长的光。 它们可用于检测免疫测定，化学测定和核酸探针测定中的化学或生物物质的浓度的存在或确定，以及用于研究大分子微结构的化学/物理探针程序。 它们中的两种或更多种也可以组合使用，或者其中一种或多种可以与其他化学发光化合物一起使用，在多通道测定中同时检测两种或多种不同的分析物。 还公开了用于制备这些奇数图案取代的化合物的新型中间体。