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    Stimulation of an immune response with antibodies labeled with the &agr;-galactosyl epitope
    73.
    发明授权
    Stimulation of an immune response with antibodies labeled with the &agr;-galactosyl epitope 有权
    用α半乳糖基表位标记的抗体刺激免疫应答

    公开(公告)号：US06472511B1

    公开(公告)日：2002-10-29

    申请号：US09599391

    申请日：2000-06-23

    申请人： Shui-on Leung ,  Zhengxing Qu

    发明人： Shui-on Leung ,  Zhengxing Qu

    IPC分类号： C07K100

    CPC分类号： C23C14/3464 ,  A61K38/00 ,  A61K39/00 ,  A61K47/6807 ,  A61K2039/505 ,  C07K16/2803 ,  C07K16/3007 ,  C07K16/3061 ,  C07K16/4266 ,  C07K2317/54 ,  C07K2319/00 ,  C23C14/10

    摘要： Humoral and cellular immune responses against tumor cells and infectious agents are induced in a mammal using an antibody that binds with an epitope of an antigen that is associated with a tumor or an infectious agent, and that contains at least one &agr;-galactosyl epitope. Such an antibody is capable of forming a complex with cells that express the target epitope and with antibodies that bind &agr;-galactosyl epitopes. Suitable antibodies include molecules that contain at least one engineered glycosylation site in the constant region of the heavy chain.

    摘要翻译： 使用与与肿瘤或感染因子相关的抗原表位结合并且含有至少一个α-半乳糖基表位的抗体在哺乳动物中诱导针对肿瘤细胞和感染因子的体液和细胞免疫应答。 这样的抗体能够与表达靶表位的细胞和结合α-半乳糖基表位的抗体形成复合物。 合适的抗体包括在重链的恒定区含有至少一个工程化糖基化位点的分子。

    Mammalian Cell Lines for Increasing Longevity and Protein Yield from a Cell Culture
    77.
    发明申请
    Mammalian Cell Lines for Increasing Longevity and Protein Yield from a Cell Culture 有权
    哺乳动物细胞系从细胞培养增加寿命和蛋白质产量

    公开(公告)号：US20120088299A1

    公开(公告)日：2012-04-12

    申请号：US13287395

    申请日：2011-11-02

    申请人： David M. Goldenberg ,  Zhengxing Qu ,  Chien-Hsing Chang ,  Edmund A. Rossi ,  Jeng-Dar Yang ,  Diane Nordstrom

    发明人： David M. Goldenberg ,  Zhengxing Qu ,  Chien-Hsing Chang ,  Edmund A. Rossi ,  Jeng-Dar Yang ,  Diane Nordstrom

    IPC分类号： C12N5/10

    CPC分类号： C07K16/2896 ,  A61K2039/505 ,  C07K14/005 ,  C07K14/4747 ,  C07K16/00 ,  C07K16/18 ,  C07K16/2803 ,  C07K16/3007 ,  C07K2317/24 ,  C07K2317/31 ,  C12N5/0694 ,  C12N15/85 ,  C12N15/86 ,  C12N2500/90 ,  C12N2501/48 ,  C12N2510/02 ,  C12N2710/20022 ,  C12N2740/10043 ,  C12P21/02

    摘要： Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hamiones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transformation under serum-free conditions. The method preferably involves eukaryotic cells, more preferably mammalian cells.

    摘要翻译： 公开了用于增加细胞培养物的寿命并允许增加蛋白质生产的组合物和方法，优选重组蛋白质，例如抗体，肽，酶，生长因子，白细胞介素，干扰素，hamiones和疫苗。 用凋亡抑制基因或载体如三重突变体Bcl-2基因转染的细胞可以在培养物中更长时间地存活，导致蛋白质生物合成的状态和产量的延长。 这样的转染细胞表现出最大的细胞密度，其等于或超过由亲本细胞系实现的最大密度。 转染的细胞也可以预先适应于无血清培养基中生长，大大减少了在无血清培养基中获得蛋白质产生所需的时间。 在某些方法中，预先调节的细胞可以在无血清条件下转化后用于蛋白质生产。 该方法优选涉及真核细胞，更优选哺乳动物细胞。

    Multivalent Carriers of Bi-Specific Antibodies
    80.
    发明申请
    Multivalent Carriers of Bi-Specific Antibodies 有权
    双特异性抗体的多价载体

    公开(公告)号：US20110223645A1

    公开(公告)日：2011-09-15

    申请号：US13089645

    申请日：2011-04-19

    申请人： Hans J. Hansen ,  William J. McBride ,  Zhengxing Qu

    发明人： Hans J. Hansen ,  William J. McBride ,  Zhengxing Qu

    IPC分类号： C12N9/96 ,  C07K17/02

    CPC分类号： C07K16/2887 ,  A61K47/6897 ,  A61K51/1027 ,  A61K51/1048 ,  A61K51/109 ,  A61K2039/505 ,  B82Y5/00 ,  C07K16/3007 ,  C07K2317/31 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/01

    摘要： Provided herein are targetable constructs that are multivalent carriers of bi-specific antibodies, i.e., each molecule of a targetable construct can serve as a carrier of two or more bi-specific antibodies. Also provided are targetable complexes formed by the association of a targetable construct with two or more bi-specific antibodies. The targetable constructs and targetable complexes of the invention are incorporated into biosensors, kits and pharmaceutical compositions, and are used in a variety of therapeutic and other methods.

    摘要翻译： 本文提供作为双特异性抗体的多价载体的可靶向构建体，即可靶向构建体的每个分子可以用作两种或更多种双特异性抗体的载体。 还提供了通过可靶向构建体与两种或更多种双特异性抗体缔合形成的可靶向复合物。 将本发明的可靶向构建体和可靶向复合物掺入生物传感器，试剂盒和药物组合物中，并且以多种治疗和其它方法使用。