公开(公告)号：US11879162B2

公开(公告)日：2024-01-23

申请号：US17810696

申请日：2022-07-05

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q1/68 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2565/101

Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US11674960B2

公开(公告)日：2023-06-13

申请号：US17344870

申请日：2021-06-10

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Axel Scherer ,  Samuel Njoroge ,  Jingqing Huang

IPC: G01N33/543 ,  G01N33/566 ,  A61B5/1459 ,  G01N21/41 ,  G01N33/552 ,  A61B5/1491

CPC classification number: G01N33/566 ,  A61B5/1459 ,  G01N21/41 ,  G01N33/54373 ,  G01N33/552 ,  A61B5/1491 ,  A61B2562/0233 ,  A61B2562/043 ,  G01N2201/068 ,  G01N2201/08

Abstract: An implantable diagnostic device in accordance with the present disclosure provides various benefits such as a compact size thereby allowing implanting of the device inside animate objects; low cost due to incorporation of inexpensive detection circuitry and the use of conventional IC fabrication techniques; re-usability by heating thereby allowing multiple diagnostic tests to be performed without discarding the device; and a configuration that allows performing of simultaneous and/or sequential diagnostic tests for detecting one or more similar or dissimilar target molecules concurrently or at different times.

公开(公告)号：US11313722B2

公开(公告)日：2022-04-26

申请号：US17093211

申请日：2020-11-09

Applicant: California Institute of Technology

Inventor： Axel Scherer ,  Taeyoon Jeon ,  Jieun Myung

IPC: G01J3/12 ,  G01J3/10 ,  G01J3/02 ,  G01J3/26

Abstract: An infrared spectrometer for operation in the mid-infrared spectral range is disclosed, where the spectrometer includes a Bragg-mirror-based spectral filter that is operative for providing an output optical signal whose spectral content is spatially dispersed along a first direction, where the Bragg mirrors include low-refractive-index layers comprising a polymer material that is transmissive across the mid-infrared spectral range and is characterized by less than ten absorption peaks with the operating spectral range of the spectrometer.

公开(公告)号：US11260943B2

公开(公告)日：2022-03-01

申请号：US16719371

申请日：2019-12-18

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. Kartalov ,  Axel Scherer

IPC: B63C11/02 ,  G01N27/22 ,  G01N21/41

Abstract: Methods and devices including implantable micro-sensors used to detect tissue-dissolved inert gas and to detect microbubble formation to avoid Caisson disease are described. The disclosed methods and devices are based on measuring the refractive index changes in hydrophobic liquids after absorbing an inert gas such as nitrogen. The changes in the refractive index are based on implementing one of an interferometry, optical microcavity resonance shift, a photonic crystal resonance, a beam deflection, a resonance tuning or detuning, an amplitude change, or an intensity change method.

公开(公告)号：US20210363581A1

公开(公告)日：2021-11-25

申请号：US17125870

申请日：2020-12-17

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer ,  Mark D. Goldberg

IPC: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/70

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US10959617B2

公开(公告)日：2021-03-30

申请号：US16204942

申请日：2018-11-29

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Axel Scherer ,  Muhammad Mujeeb-U-Rahman ,  Meisam Honavar Nazari ,  Muhammad Musab Jilani

IPC: A61B5/00 ,  A61B5/145 ,  G16H40/67 ,  A61B5/1473 ,  A61B5/1459 ,  G06F19/00

Abstract: A sensing device allows detection of biological quantities in ways that are minimally invasive. Micrometer or nanometer sized needles allow sensing of bodily fluids in a minimally invasive method. The device comprises electronics and power harvesting. Antennas or coils allow communication and power harvesting from an external device, which can be attached to smartphones to allow operation of a camera and camera light for biosensing.

公开(公告)号：US20210002732A1

公开(公告)日：2021-01-07

申请号：US16827590

申请日：2020-03-23

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US20200309676A1

公开(公告)日：2020-10-01

申请号：US16901352

申请日：2020-06-15

Applicant: California Institute of Technology

Inventor： Axel Scherer ,  Amirhossein Nateghi ,  Taeyoon Jeon ,  Frank T. Hartley

IPC: G01N21/03 ,  G01N1/42 ,  G01N1/44 ,  G01N21/17 ,  G01N21/25 ,  G01N21/35

Abstract: The present disclosure is directed toward optical elements, such as sample cuvettes, lenses, prisms, and the like, whose transmissivity is increased by the addition of a geometric anti-reflection layer disposed on at least one surface of the optical element, where the geometric anti-reflection layer includes a plurality of geometric features that collectively reduce the reflectivity of the interface between the surface and another medium. As a result, more of an optical signal incident on the surface passes through the interface. In some embodiments, every surface through which an optical signal passes includes a geometric anti-reflection layer. Due to the increased transmissivity of the optical element, in some embodiments, the use of low-cost, high-refractive-index materials, such as conventional silicon, is enabled.

公开(公告)号：US10770170B2

公开(公告)日：2020-09-08

申请号：US15914356

申请日：2018-03-07

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer

IPC: G01N33/48 ,  G01N33/50 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64 ,  C12Q1/6825 ,  G06F17/10 ,  G06F17/11 ,  G16B40/00

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US10712258B2

公开(公告)日：2020-07-14

申请号：US16212347

申请日：2018-12-06

Applicant: California Institute of Technology

Inventor： Axel Scherer ,  Amirhossein Nateghi ,  Taeyoon Jeon ,  Frank T. Hartley

IPC: G01N1/42 ,  G01N21/03 ,  G01N1/44 ,  G01N21/17 ,  G01N21/25 ,  G01N21/35 ,  G01N21/3504 ,  G01N21/3577

Abstract: The present disclosure is directed toward a cuvette for holding a test sample during optical interrogation with a light signal. The transmissivity of the cuvette is increased by a geometric anti-reflection layer disposed on at least one surface of the cuvette, where the geometric anti-reflection layer includes a plurality of geometric features that collectively reduce the reflectivity of the interface between the surface and another medium. As a result, more of the interrogation signal passes through the interface. In some embodiments, every surface through which the interrogation signal passes includes a geometric anti-reflection layer. Due to the increased transmissivity of the cuvette, light detected after passing through it can have an improved signal-to-noise ratio and/or the light signal used to interrogate the sample can have lower intensity. In addition, the reduction of the reflectivity of each surface enables the use of low-cost, high-refractive-index materials, such as conventional silicon.