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公开(公告)号：US20110306103A1
公开(公告)日：2011-12-15
申请号：US13213602
申请日：2011-08-19
IPC分类号： C12P7/40
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
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公开(公告)号：US20110305683A1
公开(公告)日：2011-12-15
申请号：US13213642
申请日：2011-08-19
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
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公开(公告)号：US08062875B2
公开(公告)日：2011-11-22
申请号：US12572094
申请日：2009-10-01
申请人： Robert DiCosimo , Mark S. Payne , Tyler Yin
发明人： Robert DiCosimo , Mark S. Payne , Tyler Yin
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are variants enzymes that are structurally classified as CE-7 enzymes and have perhydrolysis activity. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned variant enzymes as well as methods and compositions comprising the variant enzymes. Further, disinfectant formulations comprising the peroxycarboxylic acids produced by the processes described herein are provided.
摘要翻译： 本文公开了在结构上分类为CE-7酶并具有过水解活性的变体酶。 本文还公开了使用上述变体酶从羧酸酯生产过氧羧酸的方法以及包含变体酶的方法和组合物。 此外，提供包含通过本文所述方法产生的过氧羧酸的消毒剂制剂。
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公开(公告)号：US07960151B1
公开(公告)日：2011-06-14
申请号：US13036752
申请日：2011-02-28
CPC分类号： C12N9/18 , A61L2/186 , C12P7/40 , C12Y301/01072
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
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公开(公告)号：US20100168237A1
公开(公告)日：2010-07-01
申请号：US12722309
申请日：2010-03-11
CPC分类号： A01N37/02 , A01N37/16 , A01N37/36 , A01N59/00 , A61L2/186 , C12P7/40 , C12P7/62 , A01N63/02 , A01N2300/00
摘要： A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
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公开(公告)号：US20100087529A1
公开(公告)日：2010-04-08
申请号：US12572094
申请日：2009-10-01
申请人： Robert DiCosimo , Mark S. Payne , Tyler Yin
发明人： Robert DiCosimo , Mark S. Payne , Tyler Yin
IPC分类号： A01N37/00 , C07H21/04 , C12N15/63 , C12N1/21 , C12N15/74 , C12N9/16 , C12P7/40 , A61L2/16 , D06L3/00 , C11D3/39 , A01P1/00
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are variants enzymes that are structurally classified as CE-7 enzymes and have perhydrolysis activity. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned variant enzymes as well as methods and compositions comprising the variant enzymes. Further, disinfectant formulations comprising the peroxycarboxylic acids produced by the processes described herein are provided.
摘要翻译： 本文公开了在结构上分类为CE-7酶并具有过水解活性的变体酶。 本文还公开了使用上述变体酶从羧酸酯生产过氧羧酸的方法以及包含变体酶的方法和组合物。 此外，提供包含通过本文所述方法产生的过氧羧酸的消毒剂制剂。
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公开(公告)号：US20090311763A1
公开(公告)日：2009-12-17
申请号：US12497369
申请日：2009-07-02
IPC分类号： C12P7/04
CPC分类号： C11D3/38636 , A01N37/16 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/3947 , C11D3/48 , C12P7/40 , A01N37/02 , A01N37/12 , A01N37/46 , A01N43/16 , A01N59/00 , A01N61/00 , A01N2300/00
摘要： A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
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公开(公告)号：US20090239948A1
公开(公告)日：2009-09-24
申请号：US12433324
申请日：2009-04-30
申请人： Robert DiCosimo , Mark S. Payne , Vincent Brian Croud , John E. Gavagan , Lorraine W. Wagner , Eugenia Costa Hann
发明人： Robert DiCosimo , Mark S. Payne , Vincent Brian Croud , John E. Gavagan , Lorraine W. Wagner , Eugenia Costa Hann
CPC分类号： C11D3/3947 , A61L2/183 , A61L2/186 , A61L2202/18 , A61L2202/24 , A61L2202/26 , C11D3/2093 , C11D3/32 , C11D3/38627 , C11D3/48 , C12P7/00 , C12P7/40
摘要： A process is provided to produce a concentrated aqueous peracid solution in situ using at least one enzyme having perhydrolase activity in the presence of hydrogen peroxide (at a concentration of at least 500 mM) under neutral to acidic reaction conditions from suitable carboxylic acid esters (including glycerides) and/or amides substrates. The concentrated peracid solution produced is sufficient for use in a variety of disinfection and/or bleaching applications.
摘要翻译： 提供了一种方法，在中性至酸性反应条件下，使用至少一种在过氧化氢（至少500mM浓度）存在下具有过水解酶活性的酶，从合适的羧酸酯（包括 甘油酯）和/或酰胺底物。 所生产的浓缩的过酸溶液足以用于各种消毒和/或漂白应用。
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公开(公告)号：US07473545B2
公开(公告)日：2009-01-06
申请号：US11712336
申请日：2007-02-28
摘要： Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.
摘要翻译： 提供了用于从乙醇腈生产乙醇酸的各种方法。 这些方法包括：1）使用具有改进的腈水解酶活性的乙酰甘氨酸转化成乙醇酸的Acidovorax facilis 72W腈水解酶突变体，和2）提高催化剂稳定性和/或生产率的方法。 提高催化剂稳定性/生产率的方法包括使用反应稳定剂，在基本上无氧条件下进行反应，以及控制反应混合物中底物的浓度。
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90.发明申请公开(公告)号：US20090004711A1
公开(公告)日：2009-01-01
申请号：US12189906
申请日：2008-08-12
申请人： Robert DiCosimo , Anna Panova , Jeffery Scott Thompson , Robert D. Fallon , Thomas Foo , F. Glenn Gallagher , Xu Li , George C. Fox , Joseph J. Zaher , Mark S. Payne , Daniel P. O'Keefe
发明人： Robert DiCosimo , Anna Panova , Jeffery Scott Thompson , Robert D. Fallon , Thomas Foo , F. Glenn Gallagher , Xu Li , George C. Fox , Joseph J. Zaher , Mark S. Payne , Daniel P. O'Keefe
IPC分类号： C12P7/42
CPC分类号： C07C51/48 , C07C51/42 , C07C51/487 , C12P7/42 , C07C59/06
摘要： A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.
摘要翻译： 提供从甲醛和氰化氢生产乙醇酸的方法。 更具体地，使热处理的甲醛和氰化氢反应以制备具有低浓度杂质的乙醇腈。 随后使用具有源自Acidovorax facilis 72W（ATCC 57746）的腈水解酶活性的酶催化剂将乙醇腈转化为乙醇酸铵水溶液。 使用本文所述的各种方法，从乙醇酸水溶液中以酸或盐的形式回收乙醇酸。
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