公开(公告)号：US08163872B2

公开(公告)日：2012-04-24

申请号：US11577916

申请日：2005-10-27

申请人： Lene Aassveen ,  Kamilla Lundhaug ,  Kjersti Aastorp Hirth

发明人： Lene Aassveen ,  Kamilla Lundhaug ,  Kjersti Aastorp Hirth

IPC分类号： C07K1/18 ,  C07K1/30 ,  C07K9/00

CPC分类号： C07K9/008

摘要： A novel and improved method for purification of glycopeptides, especially glycopeptide antibiotics. The method comprises contacting a solution of the glycopeptide to an ion exchange chromatography material. The product of this method has a surprisingly high purity.

摘要翻译： 一种用于纯化糖肽，特别是糖肽抗生素的新颖和改进的方法。 该方法包括将糖肽的溶液与离子交换色谱材料接触。 该方法的产物具有惊人的高纯度。

公开(公告)号：US20120088906A1

公开(公告)日：2012-04-12

申请号：US13270148

申请日：2011-10-10

申请人： Fabio Polato ,  Mathias Rainer ,  Douglas T. Gjerde ,  Guenther Bonn

发明人： Fabio Polato ,  Mathias Rainer ,  Douglas T. Gjerde ,  Guenther Bonn

IPC分类号： C07K1/30 ,  C07K1/36

CPC分类号： C07K1/32

摘要： The invention provides methods and apparatus for the selective isolation of phosphorylated and glycosylated proteins and their fragments. Metal cation is used to precipitate proteins or protein fragments containing phospho groups and/or glyco groups. The sample preparation method can be used for several types of biological samples, including HeLa cells, food, and human cerebrospinal fluid. The proteins are isolated, recovered and ready for analysis by mass spectrometry or other analytical methods allowing detection limits down to the femtomole level. The method and apparatus are valuable tools in the field of protein analysis and diagnostics.

摘要翻译： 本发明提供了选择性分离磷酸化和糖基化蛋白质及其片段的方法和装置。 金属阳离子用于沉淀含有磷酸基团和/或糖基团的蛋白质或蛋白质片段。 样品制备方法可用于几种类型的生物样品，包括HeLa细胞，食物和人脑脊液。 蛋白质被分离，回收并准备好通过质谱法或其他分析方法进行分析，允许检测限降低到飞甲的水平。 该方法和装置是蛋白质分析和诊断领域的宝贵工具。

公开(公告)号：US08133521B2

公开(公告)日：2012-03-13

申请号：US11971796

申请日：2008-01-09

申请人： Ronald E. Strohbehn ,  Jesse I. Figgins

发明人： Ronald E. Strohbehn ,  Jesse I. Figgins

IPC分类号： A23J1/09 ,  A23L1/32 ,  C07K1/14 ,  C07K1/16 ,  C07K1/30 ,  C07K1/34

CPC分类号： A23K20/179 ,  A23J1/08 ,  A23J1/09 ,  A23K20/147 ,  A23K50/10 ,  A23K50/40 ,  A23K50/75 ,  A23L5/00 ,  A23L33/17 ,  A23V2002/00 ,  A23V2200/30 ,  A23V2250/5428 ,  A23V2250/211

摘要： The present invention relates to methods for the separation of various components from eggs. More particularly, the present invention relates to methods for the separation of proteins and lipids from eggs, including technical eggs (inedible) or edible eggs, yolks or whites, which comprises cross-linking the lipids of eggs with a cross-linking reagent. In an embodiment, the method includes separating the proteins from the cross-linked lipids. In an embodiment, the method includes the separation of various components associated with the cross-linked lipids. The methods disclosed herein allow for the isolation of multiple different components from the egg in an efficient, cost-effective manner without compromising the recovery process of the components or their subsequent utility in various applications or compositions. The compositions and isolated components obtained by the methods of the invention can be used in pharmaceutical, medical, nutritional, cosmetic or health applications.

摘要翻译： 本发明涉及从蛋中分离各种成分的方法。 更具体地说，本发明涉及从蛋中分离蛋白质和脂质的方法，包括技术蛋（不可食用）或食用卵，蛋黄或白蛋白，其包括用交联剂交联卵的脂质。 在一个实施方案中，该方法包括从交联的脂质中分离蛋白质。 在一个实施方案中，该方法包括分离与交联脂质相关的各种成分。 本文公开的方法允许以有效的，成本有效的方式从蛋中分离多个不同的组分，而不会影响组分的回收过程或其随后在各种应用或组合物中的应用。 通过本发明方法获得的组合物和分离组分可用于制药，医疗，营养，美容或健康应用。

公开(公告)号：US20110305817A1

公开(公告)日：2011-12-15

申请号：US13150941

申请日：2011-06-01

申请人： Seong Jun Cho ,  Sang Hyun Seo ,  Sung Wook Han ,  Je Hoon Ryu ,  Seung Won Park

发明人： Seong Jun Cho ,  Sang Hyun Seo ,  Sung Wook Han ,  Je Hoon Ryu ,  Seung Won Park

IPC分类号： A23L1/305 ,  A23J1/00 ,  C07K14/00 ,  C07K1/30 ,  C07K1/36

CPC分类号： A23J1/12 ,  A23J1/14 ,  A23V2002/00 ,  A23V2300/14 ,  A23V2300/40

摘要： The present invention relates to a method for separating protein from food by using an iso-electric point precipitation, comprising: obtaining a protein extract by adjusting pH of a raw material food in a media; precipitating the protein by adjusting pH of the protein extract to an iso-electric point of the desired protein; and recovering the protein precipitated after separating a protein filtrate through centrifuging the precipitated protein; in which the method may further include a heat-precipitation of the protein by heating the protein extract or the protein filtrate to 60˜145° C. The protein obtained in high yield according to the above method can be used as a food additive or a food nutritional enhancer.

摘要翻译： 本发明涉及通过使用异电点沉淀从蛋白质中分离蛋白质的方法，包括：通过调节培养基中原料食品的pH来获得蛋白质提取物; 通过将蛋白质提取物的pH调节到所需蛋白质的等电点来沉淀蛋白质; 并通过离心沉淀的蛋白质分离蛋白质滤液后回收沉淀的蛋白质; 其中所述方法还可以包括通过将蛋白质提取物或蛋白质滤液加热至60〜145℃来进行蛋白质的热沉淀。根据上述方法以高产率获得的蛋白质可用作食品添加剂或 食物营养增强剂。

公开(公告)号：US08075893B2

公开(公告)日：2011-12-13

申请号：US12326687

申请日：2008-12-02

申请人： Juan López De Silanes ,  Rita Guadalupe Mancilla Nava ,  Jorge F. Paniagua-Solís ,  Alejandro Alagon Cano ,  Walter J. García-Ubbelohde

发明人： Juan López De Silanes ,  Rita Guadalupe Mancilla Nava ,  Jorge F. Paniagua-Solís ,  Alejandro Alagon Cano ,  Walter J. García-Ubbelohde

IPC分类号： A61K39/395 ,  C07K1/30

CPC分类号： C07K16/065 ,  A61K2039/505 ,  C07K16/18 ,  C07K16/241 ,  C07K16/249 ,  C07K2317/54 ,  Y10S424/809

摘要： The present invention is directed to a pharmaceutical composition comprising F(ab′)2 antibody fragments that are preferably free from albumin and of whole antibodies and also substantially free of pyrogens, and an effective amount of a pharmaceutically acceptable carrier. It is also directed to a method for the production of a pharmaceutical composition comprising F(ab′)2 antibody fragments using serum or blood plasma of a mammal that has been previously immunized as a source of antibodies. The serum or blood plasma is digested with an enzyme pepsin, followed by separation and purification until the pharmaceutical composition of F(ab′)2 fragments is free of albumin and complete antibodies, and substantially free of pyrogens.

摘要翻译： 本发明涉及包含优选不含白蛋白和完全抗体也基本上不含热原的F（ab'）2抗体片段和有效量的药学上可接受的载体的药物组合物。 还涉及使用先前已被免疫作为抗体来源的哺乳动物的血清或血浆来制备包含F（ab'）2抗体片段的药物组合物的方法。 用酶胃蛋白酶消化血清或血浆，然后分离和纯化，直到F（ab'）2片段的药物组合物不含白蛋白和完全抗体，基本上不含热原。

公开(公告)号：US20100261886A1

公开(公告)日：2010-10-14

申请号：US12759016

申请日：2010-04-13

申请人： Alahari Arunakumari ,  Gisela M. Ferreira

发明人： Alahari Arunakumari ,  Gisela M. Ferreira

IPC分类号： C07K1/36 ,  C07K1/30 ,  C07K16/00

CPC分类号： C07K1/30 ,  C07K1/36

摘要： The invention provides methods for isolating proteins in purified form from mixtures by precipitation with citrate. The methods are advantageous in that they effectively separate a protein from lower molecular weight contaminants, including fragments or portions of the protein. Such methods are particularly useful for purifying antibodies from mixtures containing antibody proteolytic fragments and unpaired chains.

摘要翻译： 本发明提供了通过用柠檬酸盐沉淀从混合物中分离纯化形式的蛋白质的方法。 该方法的优点在于它们有效地将蛋白质与低分子量污染物（包括蛋白质的片段或部分）分离。 这样的方法特别可用于从含有抗体蛋白水解片段和未配对链的混合物中纯化抗体。

公开(公告)号：US20100093982A1

公开(公告)日：2010-04-15

申请号：US12520117

申请日：2007-11-27

申请人： Hans-Jurgen Henzler ,  Stephan Kirchner ,  Dirk Havekost ,  Klaus Kaiser ,  Jörg Kauling ,  Richard Pasquinelli ,  Rolf Treckmann

发明人： Hans-Jurgen Henzler ,  Stephan Kirchner ,  Dirk Havekost ,  Klaus Kaiser ,  Jörg Kauling ,  Richard Pasquinelli ,  Rolf Treckmann

IPC分类号： C07K1/30

CPC分类号： C07K1/30

摘要： The present invention relates to a method for precipitation of peptide where the mixing step of the peptide with the precipitation aid and the precipitation itself are specially separated.

摘要翻译： 本发明涉及肽的沉淀方法，其中肽与沉淀助剂的混合步骤和沉淀本身被特别分离。

公开(公告)号：US20100048873A1

公开(公告)日：2010-02-25

申请号：US12447358

申请日：2007-09-24

申请人： Martin Lotz ,  Gerold Eggengoor

发明人： Martin Lotz ,  Gerold Eggengoor

IPC分类号： C07K14/415 ,  C07K1/30

CPC分类号： A61K8/645 ,  A23J1/006 ,  A23K10/35 ,  A23K20/147 ,  A23L33/185 ,  A61Q19/00 ,  C07K1/30 ,  C07K14/415 ,  Y02P60/877

摘要： A method of preparing a coagulated plant protein fraction with a medium molecular weight of between 14 to 97, by providing fruit juice in aqueous solution; precipitating a high-molecular-weight plant protein fraction whose bulk has a molecular weight of from above 100 to 600, by adjusting an acidic pH and/or a temperature above room temperature, and mechanically separating the fraction precipitated thus; precipitating a medium-molecular-weight coagulated plant protein fraction under warm conditions by treating, at pH 2 to 7, and between 60 to 90° C., the solution obtained after separation of the high-molecular-weight plant protein fraction and mechanically separating the medium-molecular-weight coagulated plant protein fraction with a molecular weight of between approximately 14 to 97, with the bulk of the molecular weight distribution being between 20 to 60. The plant protein fraction is usable as foodstuff, food additive, additive in pharmaceuticals, animal feed, in cosmetics, as industrial protein, as adhesive.

摘要翻译： 通过在水溶液中提供果汁来制备中等分子量在14至97之间的凝固的植物蛋白质级分的方法; 通过调节酸性pH和/或高于室温的温度，并使由此沉淀的级分机械分离，沉淀其本体具有高于100至600的分子量的高分子量植物蛋白级分; 在温度条件下通过在pH2-7和60至90℃下处理中分子量凝固的植物蛋白质级分，在分离出高分子量植物蛋白质级分后获得的溶液和机械分离 分子量约为14至97的中分子量凝固植物蛋白质级分，分子量分布的大部分为20至60.植物蛋白质级分可用作食品，食品添加剂，药物中的添加剂 ，动物饲料，在化妆品中，作为工业蛋白质，作为粘合剂。

公开(公告)号：US20090292109A1

公开(公告)日：2009-11-26

申请号：US12425328

申请日：2009-04-16

申请人： Robert S. GRONKE ,  Orlando A. Jaquez

发明人： Robert S. GRONKE ,  Orlando A. Jaquez

IPC分类号： C07K1/30 ,  C07K16/00

CPC分类号： C07K1/30 ,  A61K39/39591 ,  C07K16/00 ,  C07K2317/14

摘要： The present invention is related to a method of isolating a biological macromolecule in a composition. Specifically, the present invention is related to a method of isolating a biomacromolecule in a composition containing an impurity, the method comprising adding a polyalkylene glycol to the composition, adding a transition metal to the composition, and separating said biomacromolecule from said impurity.

摘要翻译： 本发明涉及在组合物中分离生物大分子的方法。 具体地，本发明涉及在含有杂质的组合物中分离生物大分子的方法，该方法包括向组合物中加入聚亚烷基二醇，向组合物中加入过渡金属，并将所述生物大分子与所述杂质分离。

公开(公告)号：US20080139465A1

公开(公告)日：2008-06-12

申请号：US10572523

申请日：2005-06-23

申请人： Shabtai Bauer

发明人： Shabtai Bauer

IPC分类号： A61K38/00 ,  C07K1/30 ,  C12N7/06

CPC分类号： C07K14/8125 ,  A61K38/00

摘要： A process suitable for processing scaled-up amounts of source material in the range of tens of kilograms for the purification of alpha-1 proteinase inhibitor (API) from a mixture of unpurified proteins is provided. More particularly, a process for the purification of API from blood plasma or from plasma fractions to obtain pharmaceutical grade API on a commercial scale is provided. The API produced by the process is highly pure (at least 90% API out of the total protein) and highly active (at least 90% active API). Pharmaceutical compositions comprising the purified API and methods of using same are also described.

摘要翻译： 提供了一种适用于处理数十公斤范围内的源材料的放大量用于从未纯化蛋白质的混合物纯化α-1蛋白酶抑制剂（API）的方法。 更具体地，提供了从血浆或血浆级分纯化API以获得商业规模的药物级API的方法。 该方法生产的API是高纯度（总蛋白中至少90％的API）和高活性（至少90％活性的API）。 还描述了包含纯化的API的药物组合物及其使用方法。