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    POLYCATIONIC METHYL PHOSPHOLIPIDS FOR IMPROVEDDELIVERY OF NUCLEIC ACIDS TO EUKARYOTIC CELLS
    2.
    发明申请
    POLYCATIONIC METHYL PHOSPHOLIPIDS FOR IMPROVEDDELIVERY OF NUCLEIC ACIDS TO EUKARYOTIC CELLS 审中-公开
    用于改进核酸向真核细胞增殖的聚合甲基磷脂

    公开(公告)号：US20170029448A1

    公开(公告)日：2017-02-02

    申请号：US15107889

    申请日：2013-10-28

    申请人： MOLECULAR TRANSFER, INC.

    发明人： Gulilat GEBEYEHU ,  Joel JESSEE

    IPC分类号： C07F9/10 ,  C12N15/88 ,  C12N15/113 ,  C12P21/00

    CPC分类号： C07F9/10 ,  C07F9/106 ,  C07F9/6506 ,  C12N15/113 ,  C12N15/88 ,  C12N2310/14 ,  C12P21/00

    摘要： New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes. The present invention is in the field of molecular biology, and more particularly in the field of transfection, which utilizes new compounds and methods for the introduction of nucleic acids into eukaryotic cells.

    摘要翻译： 提供了新的阳离子脂质，其可用于将大分子如核酸递送到真核细胞中。 脂质可以单独使用，与其他脂质组合使用，和/或与其他转染增强试剂组合使用以制备转染复合物。 本发明涉及分子生物学领域，特别是转染领域，其利用新的化合物和将核酸引入真核细胞的方法。

    Lipids for transfection of Eukaryotic cells
    4.
    发明授权
    Lipids for transfection of Eukaryotic cells 有权
    用于转染真核细胞的脂质

    公开(公告)号：US08759499B2

    公开(公告)日：2014-06-24

    申请号：US13073972

    申请日：2011-03-28

    申请人： Joel Jessee ,  Gulilat Gebeyehu

    发明人： Joel Jessee ,  Gulilat Gebeyehu

    IPC分类号： C07D487/00 ,  A61K48/00 ,  A61K51/00 ,  A61K36/14 ,  A01N63/00

    CPC分类号： C07K14/005 ,  A61K47/6455 ,  C12N15/87 ,  C12N2720/12022

    摘要： Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.

    摘要翻译： 提供了将大分子转运到真核细胞中的组合物和方法。 来自非包膜病毒融合蛋白的融合肽提高了转染试剂如阳离子脂质，聚阳离子聚合物如PEI和树枝状大分子介导的真核细胞的转染效率。 这些融合肽用作转染复合物的一部分，其将大分子例如核酸有效地递送到真核细胞中。 还提供了新的阳离子脂质和阳离子脂质的组合物，其可用于将大分子如核酸，蛋白质和肽引入多种细胞和组织中。 脂质可以单独使用，与其他脂质组合使用和/或与融合肽组合使用以制备转染复合物。

    Ready-to-use electroporation cuvette including frozen electrocompetent cells
    10.
    发明申请
    Ready-to-use electroporation cuvette including frozen electrocompetent cells 有权
    即用型电穿孔试管，包括冷冻电感受体细胞

    公开(公告)号：US20050214946A1

    公开(公告)日：2005-09-29

    申请号：US11072715

    申请日：2005-03-07

    申请人： Robert Bebee

    发明人： Robert Bebee

    IPC分类号： C12M1/42 ,  C12M3/00 ,  C12N13/00 ,  C12N15/87

    CPC分类号： C12M35/02

    摘要： A ready-to-use electroporation cuvette is provided that includes a cuvette, first and second electrodes positioned within the cuvette and electroporation competent cells frozen in a suspension solution within the cuvette, wherein the electroporation cuvette is configured to permit electroporation of the cells when the cells are thawed. The electroporation cuvette may be sealed with a cap that may be color coded to aid the user.

    摘要翻译： 提供了一种现成的电穿孔试管，其包括比色皿，位于比色皿内的第一和第二电极以及冷藏在比色杯内的悬浮溶液中的电穿孔感受态细胞，其中电穿孔比色皿构造成允许电穿孔 细胞解冻。 电穿孔试管可以用可以用颜色编码的帽来密封以帮助使用者。