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    Lipids for transfection of Eukaryotic cells
    1.
    发明授权
    Lipids for transfection of Eukaryotic cells 有权
    用于转染真核细胞的脂质

    公开(公告)号：US08759499B2

    公开(公告)日：2014-06-24

    申请号：US13073972

    申请日：2011-03-28

    申请人： Joel Jessee ,  Gulilat Gebeyehu

    发明人： Joel Jessee ,  Gulilat Gebeyehu

    IPC分类号： C07D487/00 ,  A61K48/00 ,  A61K51/00 ,  A61K36/14 ,  A01N63/00

    CPC分类号： C07K14/005 ,  A61K47/6455 ,  C12N15/87 ,  C12N2720/12022

    摘要： Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.

    摘要翻译： 提供了将大分子转运到真核细胞中的组合物和方法。 来自非包膜病毒融合蛋白的融合肽提高了转染试剂如阳离子脂质，聚阳离子聚合物如PEI和树枝状大分子介导的真核细胞的转染效率。 这些融合肽用作转染复合物的一部分，其将大分子例如核酸有效地递送到真核细胞中。 还提供了新的阳离子脂质和阳离子脂质的组合物，其可用于将大分子如核酸，蛋白质和肽引入多种细胞和组织中。 脂质可以单独使用，与其他脂质组合使用和/或与融合肽组合使用以制备转染复合物。

    Novel reagents for transfection of eukaryotic cells
    2.
    发明申请
    Novel reagents for transfection of eukaryotic cells 有权
    用于转染真核细胞的新型试剂

    公开(公告)号：US20090023215A1

    公开(公告)日：2009-01-22

    申请号：US11434765

    申请日：2006-05-17

    申请人： Joel Jessee ,  Gulilat Gebeyehu

    发明人： Joel Jessee ,  Gulilat Gebeyehu

    IPC分类号： C12N15/00 ,  C07C211/09 ,  C07K2/00 ,  C07C211/13 ,  C12N15/63 ,  C07K7/00 ,  C07K4/00 ,  C07C53/00 ,  C07K14/00

    CPC分类号： C07K14/005 ,  A61K47/6455 ,  C12N15/87 ,  C12N2720/12022

    摘要： Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.

    摘要翻译： 提供了将大分子转运到真核细胞中的组合物和方法。 来自非包膜病毒融合蛋白的融合肽提高了转染试剂如阳离子脂质，聚阳离子聚合物如PEI和树枝状大分子介导的真核细胞的转染效率。 这些融合肽用作转染复合物的一部分，其将大分子例如核酸有效地递送到真核细胞中。 还提供了新的阳离子脂质和阳离子脂质的组合物，其可用于将大分子如核酸，蛋白质和肽引入多种细胞和组织中。 脂质可以单独使用，与其他脂质组合使用和/或与融合肽组合使用以制备转染复合物。

    Reagents for transfection of eukaryotic cells
    3.
    发明授权
    Reagents for transfection of eukaryotic cells 有权
    用于转染真核细胞的试剂

    公开(公告)号：US07915230B2

    公开(公告)日：2011-03-29

    申请号：US11434765

    申请日：2006-05-17

    申请人： Joel Jessee ,  Gulilat Gebeyehu

    发明人： Joel Jessee ,  Gulilat Gebeyehu

    IPC分类号： A61K48/00 ,  A61K38/16 ,  C12N15/00

    CPC分类号： C07K14/005 ,  A61K47/6455 ,  C12N15/87 ,  C12N2720/12022

    摘要： Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.

    摘要翻译： 提供了将大分子转运到真核细胞中的组合物和方法。 来自非包膜病毒融合蛋白的融合肽提高了转染试剂如阳离子脂质，聚阳离子聚合物如PEI和树枝状大分子介导的真核细胞的转染效率。 这些融合肽用作转染复合物的一部分，其将大分子例如核酸有效地递送到真核细胞中。 还提供了新的阳离子脂质和阳离子脂质的组合物，其可用于将大分子如核酸，蛋白质和肽引入多种细胞和组织中。 脂质可以单独使用，与其他脂质组合使用和/或与融合肽组合使用以制备转染复合物。

    NOVEL REAGENTS FOR TRANSFECTION OF EUKARYOTIC CELLS
    5.
    发明申请
    NOVEL REAGENTS FOR TRANSFECTION OF EUKARYOTIC CELLS 有权
    用于转染真核细胞的新型试剂

    公开(公告)号：US20120052574A1

    公开(公告)日：2012-03-01

    申请号：US13073972

    申请日：2011-03-28

    申请人： Joel Jessee ,  Gulilat Gebeyehu

    发明人： Joel Jessee ,  Gulilat Gebeyehu

    IPC分类号： C12N5/02 ,  C07C229/04 ,  C07K14/14 ,  C07D241/04 ,  C07K7/06 ,  C07K7/08

    CPC分类号： C07K14/005 ,  A61K47/6455 ,  C12N15/87 ,  C12N2720/12022

    摘要： Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.

    摘要翻译： 提供了将大分子转运到真核细胞中的组合物和方法。 来自非包膜病毒融合蛋白的融合肽提高了转染试剂如阳离子脂质，聚阳离子聚合物如PEI和树枝状大分子介导的真核细胞的转染效率。 这些融合肽用作转染复合物的一部分，其将大分子例如核酸有效地递送到真核细胞中。 还提供了新的阳离子脂质和阳离子脂质的组合物，其可用于将大分子如核酸，蛋白质和肽引入多种细胞和组织中。 脂质可以单独使用，与其他脂质组合使用和/或与融合肽组合使用以制备转染复合物。

    Novel reagents for intracellular delivery of macromolecules
    9.
    发明申请
    Novel reagents for intracellular delivery of macromolecules 失效
    用于大分子细胞内转运的新型试剂

    公开(公告)号：US20050260757A1

    公开(公告)日：2005-11-24

    申请号：US11192758

    申请日：2005-07-29

    申请人： Gulilat Gebeyehu ,  Joel Jessee

    发明人： Gulilat Gebeyehu ,  Joel Jessee

    IPC分类号： A61K48/00 ,  C12N15/88 ,  C07J1/00

    CPC分类号： A61K48/00 ,  A61K47/543 ,  C07H1/00

    摘要： The present invention provides certain cationic lipids containing stigmasterol, ergosterol and cholic acid groups. Compounds of the invention are useful, either alone or in combination with other lipid aggregate-forming components (e.g., DOPE, DOSPA, DOTMA or cholesterol) for formulation into liposomes or other lipid aggregates. Such aggregates are cationic, and able to form stable complexes with anionic macromolecules, such as nucleic acids. The cationic lipids of the invention are useful in methods of transfecting cells, particularly to introduce nucleic acids into cells. The invention also related to kits for the preparation of lipid aggregates and to lipid aggregates and compositions for transfection of cells.

    摘要翻译： 本发明提供了含有豆甾醇，麦角固醇和胆酸基团的某些阳离子脂质。 本发明的化合物单独使用或与其它脂质聚集体形成组分（例如，DOPE，DOSPA，DOTMA或胆固醇）组合用于制剂成脂质体或其它脂质聚集体。 这种聚集体是阳离子的，能够与阴离子大分子如核酸形成稳定的络合物。 本发明的阳离子脂质可用于转染细胞的方法，特别是将核酸引入细胞中。 本发明还涉及用于制备脂质聚集体和用于转染细胞的脂质聚集体和组合物的试剂盒。