公开(公告)号：US10450569B2

公开(公告)日：2019-10-22

申请号：US15752182

申请日：2016-08-12

申请人： SOMAGENICS, INC.

发明人： Anne Dallas ,  Heini Ilves ,  Sumedha Jayasena ,  Brian H. Johnston

IPC分类号： C12N15/113 ,  A61K31/713 ,  C07H21/02 ,  A61P17/02 ,  A61K45/06

摘要： Wound healing is a complex homeostatic process in which several distinct types coordinate to repair a physical damage. Failure to close wounds contributes to the pathology of conditions like diabetes mellitus, particularly in the elderly. Presented herein are molecules, pharmaceutical compositions, and methods for applying small RNA oligonucleotide technology to wound healing. Small RNA oligonucleotide approaches as disclosed herein provide a therapeutic strategy for improving both basal and pathological wound healing.

公开(公告)号：US09809847B2

公开(公告)日：2017-11-07

申请号：US15318640

申请日：2015-06-19

申请人： SomaGenics, Inc.

发明人： Sergei A. Kazakov ,  Catharina Casper-Lindley ,  Anne Dallas ,  Heini Ilves ,  Brian H. Johnston

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q2521/319 ,  C12Q2521/501 ,  C12Q2525/121 ,  C12Q2525/186 ,  C12Q2531/125 ,  C12Q2535/101 ,  C12Q2563/131 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2531/113 ,  C12Q2535/122

摘要： Disclosed herein are compositions and methods for the processing, amplification and detection of polynucleotides using target-specific oligonucleotides (TSOs). Hybridization of TSOs to target polynucleotides guides target processing into and purification of small target fragments that then can be amplified and detected with high sensitivity and reproducibility. The method is specifically beneficial for highly degraded polynucleotides found in biological samples.

公开(公告)号：US20150051099A1

公开(公告)日：2015-02-19

申请号：US14367200

申请日：2012-12-21

申请人： SomaGenics, Inc.

发明人： Sergei A. Kazakov

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6837 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6813 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2525/207 ,  C12Q2539/101 ,  C12Q2525/186 ,  C12Q2525/301 ,  C12Q2525/307

摘要： Methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) are disclosed herein. Methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) can be used to attach adapters and/or linkers to target RNAs. Methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) can be used in reactions, including, but not limited to, ligation reactions, amplification reactions, and sequencing reactions. Additionally, methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) can be used for reducing and/or preventing the formation of secondary structures in target RNAs. These methods, compositions, and kits can also find use in a number of applications, for example, any application that benefits from stabilizing primary RNA structure, such as detecting and quantifying target RNAs in a sample, in the construction of small RNA libraries, in microarray and RT-qPCR applications, etc.

摘要翻译： 本文公开了包含靶特异性寡核苷酸（TSO）的方法，组合物和试剂盒。 包含靶特异性寡核苷酸（TSO）的方法，组合物和试剂盒可用于将衔接子和/或连接体连接至靶RNA。 包括靶特异性寡核苷酸（TSO）的方法，组合物和试剂盒可用于反应，包括但不限于连接反应，扩增反应和测序反应。 另外，包含靶特异性寡核苷酸（TSO）的方法，组合物和试剂盒可用于减少和/或预防靶RNA中二级结构的形成。 这些方法，组合物和试剂盒也可用于许多应用，例如从稳定初级RNA结构中获益的任何应用，例如检测和定量样品中的靶RNA，构建小RNA文库 微阵列和RT-qPCR应用等

公开(公告)号：US20090170794A1

公开(公告)日：2009-07-02

申请号：US11662506

申请日：2005-09-12

申请人： Roger L. Kaspar ,  Heine Ilves ,  Attila A. Seyhan ,  Alexander V. Vlassov ,  Brian H. Johnston

发明人： Roger L. Kaspar ,  Heine Ilves ,  Attila A. Seyhan ,  Alexander V. Vlassov ,  Brian H. Johnston

IPC分类号： A61K31/7105 ,  C12N7/01 ,  C07H21/02

CPC分类号： C12N15/1131 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2770/24211 ,  C12N2770/36111

摘要： The invention provides methods, compositions, and kits comprising small interfering RNA (shRNA or siRNA) which are useful for inhibition of viralmediated gene expression. Small interfering RNAs as described herein may be used in methods of treatment of HCV infection. ShRNA and siRNA constructs that target the internal ribosome entry site (IRES) sequence of HCV are described.

摘要翻译： 本发明提供了可用于抑制病毒介导的基因表达的小干扰RNA（shRNA或siRNA）的方法，组合物和试剂盒。 如本文所述的小干扰RNA可用于治疗HCV感染的方法。 描述了靶向HCV的内部核糖体进入位点（IRES）序列的ShRNA和siRNA构建体。

公开(公告)号：US20060074041A1

公开(公告)日：2006-04-06

申请号：US11228705

申请日：2005-09-16

申请人： Brian Johnston ,  Sergei Kazakov ,  Kevin Kisich

发明人： Brian Johnston ,  Sergei Kazakov ,  Kevin Kisich

IPC分类号： A61K48/00 ,  C07H21/02

CPC分类号： C12N15/113 ,  A61K38/00 ,  C07H21/00 ,  C12N15/1136 ,  C12N15/1138 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2310/152 ,  C12N2310/315 ,  C12N2310/3511 ,  C12N2310/53 ,  C12Q1/6813

摘要： The present invention is directed to novel nucleic acid molecules and methods for their use. More specifically, the novel nucleic acid molecules of the present invention are capable of tightly and specifically interacting with a target molecule of interest not only through standard Watson-Crick base pairing, but also through additional features which allow the antisense molecules to become topologically “locked” onto the target nucleic acid, thereby imparting improved transcription and translation inhibitory properties.

摘要翻译： 本发明涉及新的核酸分子及其使用方法。 更具体地说，本发明的新型核酸分子不仅可以通过标准的沃森 - 克里克碱基配对，而且通过允许反义分子变得拓扑“锁定”的附加特征，与目标靶分子紧密而特异地相互作用 “到靶核酸上，从而赋予改善的转录和翻译抑制性质。

公开(公告)号：US11319538B2

公开(公告)日：2022-05-03

申请号：US16562118

申请日：2019-09-05

申请人： SOMAGENICS, INC.

发明人： Anne Dallas ,  Heini Ilves ,  Sumedha Jayasena ,  Brian H. Johnston

IPC分类号： C12N15/113 ,  A61K31/713 ,  C07H21/02 ,  A61K45/06 ,  A61P17/02

摘要： Wound healing is a complex homeostatic process in which several distinct types coordinate to repair a physical damage. Failure to close wounds contributes to the pathology of conditions like diabetes mellitus, particularly in the elderly. Presented herein are molecules, pharmaceutical compositions, and methods for applying small RNA oligonucleotide technology to wound healing. Small RNA oligonucleotide approaches as disclosed herein provide a therapeutic strategy for improving both basal and pathological wound healing.

公开(公告)号：US08871730B2

公开(公告)日：2014-10-28

申请号：US13384201

申请日：2010-07-13

申请人： Qing Ge ,  Brian H. Johnston ,  Mark A. Behlke ,  Heini Ilves ,  Anne Dallas

发明人： Qing Ge ,  Brian H. Johnston ,  Mark A. Behlke ,  Heini Ilves ,  Anne Dallas

IPC分类号： A01N43/04 ,  C12N15/00 ,  A61K31/7125 ,  C12N15/11

CPC分类号： C12N15/113 ,  A61K31/7088 ,  A61K31/7125 ,  A61K47/554 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/315 ,  C12N2310/318 ,  C12N2310/321 ,  C12N2310/344 ,  C12N2310/346 ,  C12N2310/3515 ,  C12N2310/531 ,  C12N2320/30 ,  C12N2320/53 ,  C12N2310/3521

摘要： Aspects of the present invention include the production and use of chemically modified RNAi agents (e.g., shRNAs) in gene silencing applications. The chemically modified RNAi agents disclosed herein have reduced immunostimulatory activity, increased serum stability, or both, as compared to a corresponding RNAi agent not having the chemical modification. Compositions containing chemically modified RNAi agents according to aspects of the present invention (including pharmaceutical compositions) and kits containing the same are also provided.

摘要翻译： 本发明的方面包括在基因沉默应用中生产和使用化学修饰的RNAi试剂（例如shRNA）。 与不具有化学修饰的相应的RNAi剂相比，本文公开的化学修饰的RNAi剂具有降低的免疫刺激活性，增加的血清稳定性或两者。 还提供了包含根据本发明的方面（包括药物组合物）的化学修饰的RNAi剂的组合物和含有该组合物的试剂盒。

公开(公告)号：US08779115B2

公开(公告)日：2014-07-15

申请号：US13591433

申请日：2012-08-22

申请人： Qing Ge ,  Brian H. Johnston ,  Sergei A Kazakov ,  Heini Ilves ,  Anne Dallas

发明人： Qing Ge ,  Brian H. Johnston ,  Sergei A Kazakov ,  Heini Ilves ,  Anne Dallas

IPC分类号： C07H21/04

CPC分类号： C12N15/111 ,  C12N15/1131 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2310/533

摘要： Methods, compositions, and kits that include small hairpin RNA (shRNA) useful for inhibition of gene expression, such as viral-mediated gene expression, are described.

摘要翻译： 描述了包括用于抑制基因表达的小发夹RNA（shRNA）的方法，组合物和试剂盒，例如病毒介导的基因表达。

公开(公告)号：US20120220033A1

公开(公告)日：2012-08-30

申请号：US13360442

申请日：2012-01-27

申请人： ROGER L. KASPAR ,  HEINI ILVES ,  ATTILA A. SEYHAN ,  ALEXANDER V. VLASSOV ,  BRIAN H. JOHNSTON

发明人： ROGER L. KASPAR ,  HEINI ILVES ,  ATTILA A. SEYHAN ,  ALEXANDER V. VLASSOV ,  BRIAN H. JOHNSTON

IPC分类号： C12N5/02 ,  C12N15/113

CPC分类号： C12N15/1131 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2770/24211 ,  C12N2770/36111

摘要： The invention provides methods, compositions, and kits comprising small interfering RNA (shRNA or siRNA) that are useful for inhibition of viral-mediated gene expression. Small interfering RNAs as described herein can be used in methods of treatment of HCV infection. ShRNA and siRNA constructs targetING the internal ribosome entry site (IRES) sequence of HCV are described.

摘要翻译： 本发明提供了可用于抑制病毒介导的基因表达的小干扰RNA（shRNA或siRNA）的方法，组合物和试剂盒。 本文所述的小干扰RNA可用于治疗HCV感染的方法。 描述了靶向HCV的内部核糖体进入位点（IRES）序列的ShRNA和siRNA构建体。

公开(公告)号：US07902351B2

公开(公告)日：2011-03-08

申请号：US11444901

申请日：2006-06-01

申请人： Roger L. Kaspar ,  Heini Ilves ,  Attila A. Seyhan ,  Alexander V. Vlassov ,  Brian H. Johnston

发明人： Roger L. Kaspar ,  Heini Ilves ,  Attila A. Seyhan ,  Alexander V. Vlassov ,  Brian H. Johnston

IPC分类号： C07H21/04

CPC分类号： C12N15/1131 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2770/24211 ,  C12N2770/36111

摘要： The invention provides methods, compositions, and kits comprising small interfering RNA (shRNA or siRNA) that are useful for inhibition of viral-mediated gene expression. Small interfering RNAs as described herein can be used in methods of treatment of HCV infection. ShRNA and siRNA constructs targetING the internal ribosome entry site (IRES) sequence of HCV are described.

摘要翻译： 本发明提供了可用于抑制病毒介导的基因表达的小干扰RNA（shRNA或siRNA）的方法，组合物和试剂盒。 本文所述的小干扰RNA可用于治疗HCV感染的方法。 描述了靶向HCV的内部核糖体进入位点（IRES）序列的ShRNA和siRNA构建体。