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公开(公告)号：US10398149B2
公开(公告)日：2019-09-03
申请号：US14428284
申请日：2013-09-10
发明人： Yan Zheng , Ruihua Guo , Tiankui Yang , Xuebing Xu
摘要： A flour product whitener containing polysaccharide alcohol. When the flour product whitener is used in a flour product, the amount of the polysaccharide alcohol is 0.01-10 parts by mass in every 100 parts by mass of total flour in the flour product.
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公开(公告)号：US11618865B2
公开(公告)日：2023-04-04
申请号：US16957618
申请日：2018-12-24
发明人： Xiang Chen , Yan Zheng , Yongqing Chi
IPC分类号： C11C3/06 , A23L33/115 , A23G1/36 , C11C3/10
摘要： The present disclosure relates to an oil and fat composition, use thereof and a food comprising the oil and fat composition. The oil and fat composition of the disclosure comprises 50-99.5 wt % triglyceride and 0.5-50 wt % wax by the total weight of the composition, the triglyceride is represented by the following formula (1), and the definitions of the groups in formula (1) are as recorded in the description. The oil and fat composition is suitable for preparation of a food.
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公开(公告)号：US10144919B2
公开(公告)日：2018-12-04
申请号：US15035105
申请日：2014-11-04
发明人： Jun Xu , Yaoji Xuan , Jinmin Li
摘要： The present application provides mutants of wild type phospholipase C (PLC) specific to phosphatidylcholine from Bacillus cereus. The related mutations include mutation of asparagine at position 63 to another amino acid, also including mutation of arginine at position 20 to histidine and of alanine at position 83 to aspartic acid. The present application also provides a nucleic acid molecule encoding the mutant, a vector containing the nucleic acid molecule, and a cell containing the nucleic acid molecule or vector. The present application also provides uses of the mutant, nucleic acid molecule vector, and cell.
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公开(公告)号：US20170327807A1
公开(公告)日：2017-11-16
申请号：US15529657
申请日：2015-11-27
CPC分类号： C12N9/2445 , C07K2319/00 , C07K2319/02 , C12N9/58 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y304/23 , C12Y304/23023
摘要： Provided is a method for recombinant expression of β-glucosidase gene. Also provided is a recombinant expression vector comprising: (a) a coding sequence of an aspartic protease or active fragment thereof, (b) a coding sequence of β-glucosidase or active fragment thereof, and optionally (c) a linker sequence between (a) and (b). Further provided are the recombinant host cell and the recombinant cellulose-degrading microorganism comprising the recombinant expression vector, the preparation method and uses thereof.
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5.发明授权Method for selective preparation of fatty acid mono- and di-esters of erythritol 有权选择性制备赤藓醇脂肪酸单酯和二酯的方法公开(公告)号：US09169191B2
公开(公告)日：2015-10-27
申请号：US14403509
申请日：2013-05-24
发明人： Zonghui Ma , Yan Zheng , Tiankui Yang , Xuebing Xu
CPC分类号： C07C67/08 , A23L27/72 , A23L29/10 , A23P10/30 , A61K8/11 , A61K8/375 , A61K2800/412 , A61Q19/00 , C07C69/33 , C07C69/58 , C07C69/003
摘要： The invention provides a method of preparing β-erythritol fatty acid monoester, including: subjecting a fatty acid and erythritol to esterification reaction in the presence of an acid catalyst, a water carrier and an optional phase transfer catalyst, wherein the molar ratio of the fatty acid to erythritol is 1:2 to 1:3, and the temperature of the esterification reaction is 80-100° C. In addition, the invention further provides a method of preparing 2,3-erythritol fatty acid diester, including: subjecting a fatty acid and erythritol to esterification reaction in the presence of an acid catalyst and a water carrier, wherein the molar ratio of the fatty acid to erythritol is 2:1 to 3:1, and the temperature of the esterification reaction is 120-160° C.
摘要翻译： 本发明提供一种制备赤藓糖醇脂肪酸单酯的方法，包括：在酸催化剂，水载体和任选的相转移催化剂存在下，使脂肪酸和赤藓糖醇进行酯化反应，其中， 脂肪酸与赤藓糖醇的比例为1:2〜1:3，酯化反应温度为80〜100℃。此外，本发明还提供了制备2,3-赤藓糖醇脂肪酸二酯的方法，包括： 脂肪酸和赤藓糖醇在酸催化剂和水载体的存在下酯化反应，其中脂肪酸与赤藓糖醇的摩尔比为2:1至3:1，酯化反应温度为120-160℃ C。
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公开(公告)号：US20220073889A1
公开(公告)日：2022-03-10
申请号：US17419082
申请日：2019-12-27
发明人： Yaoji XUAN , Qiwen NIU , Zhengjun XU
摘要： Provided is a polypeptide having phospholipase C activity. The polypeptide has: 1) an amino acid sequence represented by SEQ ID No: 2, comprising amino acid substitutions occurred at one or more positions, wherein the one or more positions are selected from positions 6, 8, 10, 104, and 205 in the amino acid sequence represented by SEQ ID No: 2 or any combination thereof; or 2) having at least 80% sequence identity with 1), and at least one of positions 6, 8, 10, 104 and 205 being different from positions 6, 8, 10, 104, and 205 in the amino acid sequence represented by SEQ ID No: 2. Provided are a nucleic acid molecule for encoding the polypeptide, a vector comprising the nucleic acid molecule, and a cell comprising the nucleic acid molecule or the vector. Provided are uses of the polypeptide, the nucleic acid molecule, the vector, and the cell.
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公开(公告)号：US09969995B2
公开(公告)日：2018-05-15
申请号：US15529657
申请日：2015-11-27
CPC分类号： C12N9/2445 , C07K2319/00 , C07K2319/02 , C12N9/58 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y304/23 , C12Y304/23023
摘要： Provided is a method for recombinant expression of β-glucosidase gene. Also provided is a recombinant expression vector comprising: (a) a coding sequence of an aspartic protease or active fragment thereof, (b) a coding sequence of β-glucosidase or active fragment thereof, and optionally (c) a linker sequence between (a) and (b). Further provided are the recombinant host cell and the recombinant cellulose-degrading microorganism comprising the recombinant expression vector, the preparation method and uses thereof.
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公开(公告)号：US20220162543A1
公开(公告)日：2022-05-26
申请号：US17419122
申请日：2019-12-27
发明人： Wei Wu , Xiaojun Dai , Haisheng Cao , Meifeng Zhou , Qiwen Niu
摘要： Provided is a Pichia pastoris mutant strain for expressing an exogenous gene. Specifically, provided is a Pichia pastoris mutant strain comprising, with respect to Pichia pastoris mutant strain GS115 or CICC32806, one or more of the following six mutations: BQ9382_C1-2260, EKK deletions at positions 308-310, a hypothetical protein; BQ9382_C1-3800, E129K, 60S ribosomal subunit assembly/exported protein LOC1; BQ9382_C1-5700, I312M, mitochondrial external NADH dehydrogenase, type II NAD(P)H:quinone oxidoreductase; BQ9382_C2-3950, Q145X, an essential protein having a binding partner Psr1p and used for completely activating a general stress response; BQ9382_C3-2220, E188K, a hypothetical protein; and BQ9382_C3-4370, W196X, orotidine 5\′-phosphate decarboxylase. The provided Pichia pastoris mutant strain is an effective commonly employed host for exogenous expression, and can efficiently express different proteins, especially phospholipase and lipase.
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公开(公告)号：US11566204B2
公开(公告)日：2023-01-31
申请号：US16771447
申请日：2018-12-10
发明人： Wei Wu , Xiaojun Dai , Haisheng Cao , Qiwen Niu
摘要： According to the present disclosure, on the basis of all existing mutations, the tenth glycine of a BC-PC-PLC is mutated into aspartic acid, a specific enzyme activity thereof is 83% higher than that of a sequence before the mutation, and protein expression and degumming activity of unit enzyme activity do not change, so as to further reduce manufacturing costs.
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公开(公告)号：US20220064611A1
公开(公告)日：2022-03-03
申请号：US17419091
申请日：2019-12-27
发明人： Yaoji XUAN , Qiwen NIU , Zhengjun XU
摘要： Provided in the present invention is a phospholipase C mutant with high enzyme activity, a polypeptide with the sequence as shown in SEQ ID NO:7, or a polypeptide derived from the phospholipase C formed by performing substitution, deletion or addition of one or a plurality of amino acids on the polypeptide of SEQ ID NO:7 while retaining the phospholipase C activity provided by SEQ ID NO:7. The phospholipase C mutant of the present invention can improve degumming efficiency and increase the yield of diacylglycerol (DAG) during degumming.
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