Isotachophoretic focusing of nucleic acids
    1.
    发明授权
    Isotachophoretic focusing of nucleic acids 有权
    核酸的同步聚焦聚焦

    公开(公告)号:US08846314B2

    公开(公告)日:2014-09-30

    申请号:US12716142

    申请日:2010-03-02

    摘要: A method and system are presented for fast and efficient isolation, purification and quantitation of nucleic acids from complex biological samples using isotachophoresis in microchannels. In an embodiment, a sieving medium may be used to enhance selectivity. In another embodiment, PCR-friendly chemistries are used to purify nucleic acids from complex biological samples and yield nucleic acids ready for further analysis including for PCR. In another embodiment, small RNAs from biological samples are extracted, isolated, preconcentrated and quantitated using on-chip ITP with a high efficiency sieving medium. The invention enables fast concentration and separation (takes 10s to 100s of seconds) of nucleic acids with high selectivity and using lower volumes of reagents (order of 10s of μL to focus less than 1 pg/μL of nucleic acid).

    摘要翻译: 提出了一种方法和系统,用于在微通道中使用等速电泳,从复杂生物样品中快速有效地分离,纯化和定量核酸。 在一个实施方案中,可以使用筛分介质来增强选择性。 在另一个实施方案中,使用PCR友好的化学物质从复杂的生物样品中纯化核酸,并产生准备用于进一步分析的核酸,包括用于PCR。 在另一个实施方案中,使用具有高效筛选培养基的片上ITP,提取,分离,预浓缩和定量来自生物样品的小RNA。 本发明使得能够以高选择性快速浓缩和分离(需要10秒至100秒)的核酸,并且使用较低体积的试剂(10μL的浓度以聚焦小于1pg /μL的核酸)。

    Record based code structure
    3.
    发明授权
    Record based code structure 有权
    基于记录的代码结构

    公开(公告)号:US08171045B2

    公开(公告)日:2012-05-01

    申请号:US12183823

    申请日:2008-07-31

    申请人: Dustin Kurt Adler

    发明人: Dustin Kurt Adler

    IPC分类号: G06F17/30

    CPC分类号: G06F17/30

    摘要: The compiled code of a computer program is stored in multiple pieces within a database. Each piece is optionally stored within a separate data record. Execution of the computer program includes using database queries to retrieve pieces of the compiled code for execution. The database and associated database management logic are used to provide numerous advantages in execution and management of the computer program. For example, in some embodiments, database queries are used to help facilitate program flow logic.

    摘要翻译: 计算机程序的编译代码存储在数据库中的多个部分中。 每个片段可选地存储在单独的数据记录中。 计算机程序的执行包括使用数据库查询来检索编译代码以供执行。 数据库和相关联的数据库管理逻辑用于在计算机程序的执行和管理中提供许多优点。 例如,在一些实施例中,使用数据库查询来帮助促进程序流逻辑。