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公开(公告)号：US20100224494A1
公开(公告)日：2010-09-09
申请号：US12716142
申请日：2010-03-02
IPC分类号： B01D57/02
CPC分类号： G01N27/447 , B01D57/02 , C12N15/101
摘要： A method and system are presented for fast and efficient isolation, purification and quantitation of nucleic acids from complex biological samples using isotachophoresis in microchannels. In an embodiment, a sieving medium may be used to enhance selectivity. In another embodiment, PCR-friendly chemistries are used to purify nucleic acids from complex biological samples and yield nucleic acids ready for further analysis including for PCR. In another embodiment, small RNAs from biological samples are extracted, isolated, preconcentrated and quantitated using on-chip ITP with a high efficiency sieving medium. The invention enables fast concentration and separation (takes 10s to 100s of seconds) of nucleic acids with high selectivity and using lower volumes of reagents (order of 10s of μL to focus less than 1 pg/μL of nucleic acid).
摘要翻译： 提出了一种方法和系统，用于在微通道中使用等速电泳，从复杂生物样品中快速有效地分离，纯化和定量核酸。 在一个实施方案中，可以使用筛分介质来增强选择性。 在另一个实施方案中，使用PCR友好的化学物质从复杂的生物样品中纯化核酸，并产生准备用于进一步分析的核酸，包括用于PCR。 在另一个实施方案中，使用具有高效筛选培养基的片上ITP，提取，分离，预浓缩和定量来自生物样品的小RNA。 本发明使得能够以高选择性快速浓缩和分离（需要10秒至100秒）的核酸，并且使用较低体积的试剂（10μL的浓度以聚焦小于1pg /μL的核酸）。
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公开(公告)号：US08846314B2
公开(公告)日：2014-09-30
申请号：US12716142
申请日：2010-03-02
CPC分类号： G01N27/447 , B01D57/02 , C12N15/101
摘要： A method and system are presented for fast and efficient isolation, purification and quantitation of nucleic acids from complex biological samples using isotachophoresis in microchannels. In an embodiment, a sieving medium may be used to enhance selectivity. In another embodiment, PCR-friendly chemistries are used to purify nucleic acids from complex biological samples and yield nucleic acids ready for further analysis including for PCR. In another embodiment, small RNAs from biological samples are extracted, isolated, preconcentrated and quantitated using on-chip ITP with a high efficiency sieving medium. The invention enables fast concentration and separation (takes 10s to 100s of seconds) of nucleic acids with high selectivity and using lower volumes of reagents (order of 10s of μL to focus less than 1 pg/μL of nucleic acid).
摘要翻译： 提出了一种方法和系统，用于在微通道中使用等速电泳，从复杂生物样品中快速有效地分离，纯化和定量核酸。 在一个实施方案中，可以使用筛分介质来增强选择性。 在另一个实施方案中，使用PCR友好的化学物质从复杂的生物样品中纯化核酸，并产生准备用于进一步分析的核酸，包括用于PCR。 在另一个实施方案中，使用具有高效筛选培养基的片上ITP，提取，分离，预浓缩和定量来自生物样品的小RNA。 本发明使得能够以高选择性快速浓缩和分离（需要10秒至100秒）的核酸，并且使用较低体积的试剂（10μL的浓度以聚焦小于1pg /μL的核酸）。
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3.发明授权公开(公告)号：US08702948B2
公开(公告)日：2014-04-22
申请号：US13618228
申请日：2012-09-14
申请人： Mostafa Ronaghi , Tarun Khurana , Juan G. Santiago
发明人： Mostafa Ronaghi , Tarun Khurana , Juan G. Santiago
CPC分类号： B01L3/502761 , B01J2219/00317 , B01J2219/00459 , B01J2219/00466 , B01J2219/00596 , B01J2219/00648 , B01J2219/00653 , B01J2219/00702 , B01L2200/0668 , B01L2300/0819 , B01L2400/0415
摘要： Disclosed are a method and apparatus that use an electric field for improved biological assays. The electric field is applied across a device having wells, which receive reactants, which carry a charge. The device thus uses a controllable voltage source between the first and second electrodes, which is controllable to provide a positive charge and a negative charge to a given electrode. By controlled use of the electric field charged species in a fluid in a fluid channel are directed into or out of the well by an electric field between the electrodes. The present method involves the transport of fluids, as in a microfluidic device, and the electric field-induced movement of reactive species according to various assay procedures, such as DNA sequencing, synthesis or the like.
摘要翻译： 公开了使用电场进行改进的生物测定的方法和装置。 电场被施加在具有井的装置上，该装置具有承载电荷的反应物。 因此，该器件在第一和第二电极之间使用可控电压源，其可控制以向给定电极提供正电荷和负电荷。 通过控制使用流体通道中的流体中的电场带电物质通过电极之间的电场被引导进入或流出阱。 本方法涉及如在微流体装置中的流体的运输，以及根据各种测定程序（例如DNA测序，合成等）的反应物种的电场诱导运动。
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4.发明申请公开(公告)号：US20090032401A1
公开(公告)日：2009-02-05
申请号：US12170941
申请日：2008-07-10
申请人： Mostafa Ronaghi , Tarun Khurana , Juan G. Santiago
发明人： Mostafa Ronaghi , Tarun Khurana , Juan G. Santiago
IPC分类号： B01D59/42
CPC分类号： B01L3/502761 , B01J2219/00317 , B01J2219/00459 , B01J2219/00466 , B01J2219/00596 , B01J2219/00648 , B01J2219/00653 , B01J2219/00702 , B01L2200/0668 , B01L2300/0819 , B01L2400/0415
摘要： Disclosed are a method and apparatus that use an electric field for improved biological assays. The electric field is applied across a device having wells, which receive reactants, which carry a charge. The device thus uses a controllable voltage source between the first and second electrodes, which is controllable to provide a positive charge and a negative charge to a given electrode. By controlled use of the electric field charged species in a fluid in a fluid channel are directed into or out of the well by an electric field between the electrodes. The present method involves the transport of fluids, as in a microfluidic device, and the electric field-induced movement of reactive species according to various assay procedures, such as DNA sequencing, synthesis or the like.
摘要翻译： 公开了使用电场进行改进的生物测定的方法和装置。 电场被施加在具有井的装置上，该装置具有承载电荷的反应物。 因此，该器件在第一和第二电极之间使用可控电压源，其可控制以向给定电极提供正电荷和负电荷。 通过控制使用流体通道中的流体中的电场带电物质通过电极之间的电场被引导进入或离开阱。 本方法涉及如在微流体装置中的流体的运输，以及根据各种测定程序（例如DNA测序，合成等）的反应物种的电场诱导运动。
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5.发明申请公开(公告)号：US20130008789A1
公开(公告)日：2013-01-10
申请号：US13618228
申请日：2012-09-14
申请人： Mostafa Ronaghi , Tarun Khurana , Juan G. Santiago
发明人： Mostafa Ronaghi , Tarun Khurana , Juan G. Santiago
CPC分类号： B01L3/502761 , B01J2219/00317 , B01J2219/00459 , B01J2219/00466 , B01J2219/00596 , B01J2219/00648 , B01J2219/00653 , B01J2219/00702 , B01L2200/0668 , B01L2300/0819 , B01L2400/0415
摘要： Disclosed are a method and apparatus that use an electric field for improved biological assays. The electric field is applied across a device having wells, which receive reactants, which carry a charge. The device thus uses a controllable voltage source between the first and second electrodes, which is controllable to provide a positive charge and a negative charge to a given electrode. By controlled use of the electric field charged species in a fluid in a fluid channel are directed into or out of the well by an electric field between the electrodes. The present method involves the transport of fluids, as in a microfluidic device, and the electric field-induced movement of reactive species according to various assay procedures, such as DNA sequencing, synthesis or the like.
摘要翻译： 公开了使用电场进行改进的生物测定的方法和装置。 电场被施加在具有井的装置上，该装置具有承载电荷的反应物。 因此，该器件在第一和第二电极之间使用可控电压源，其可控制以向给定电极提供正电荷和负电荷。 通过控制使用流体通道中的流体中的电场带电物质通过电极之间的电场被引导进入或离开阱。 本方法涉及如在微流体装置中的流体的运输，以及根据各种测定程序（例如DNA测序，合成等）的反应物种的电场诱导运动。
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6.发明授权公开(公告)号：US08277628B2
公开(公告)日：2012-10-02
申请号：US12170941
申请日：2008-07-10
申请人： Mostafa Ronaghi , Tarun Khurana , Juan G. Santiago
发明人： Mostafa Ronaghi , Tarun Khurana , Juan G. Santiago
IPC分类号： G01N27/26
CPC分类号： B01L3/502761 , B01J2219/00317 , B01J2219/00459 , B01J2219/00466 , B01J2219/00596 , B01J2219/00648 , B01J2219/00653 , B01J2219/00702 , B01L2200/0668 , B01L2300/0819 , B01L2400/0415
摘要： Disclosed are a method and apparatus that use an electric field for improved biological assays. The electric field is applied across a device having wells, which receive reactants, which carry a charge. The device thus uses a controllable voltage source between the first and second electrodes, which is controllable to provide a positive charge and a negative charge to a given electrode. By controlled use of the electric field charged species in a fluid in a fluid channel are directed into or out of the well by an electric field between the electrodes. The present method involves the transport of fluids, as in a microfluidic device, and the electric field-induced movement of reactive species according to various assay procedures, such as DNA sequencing, synthesis or the like.
摘要翻译： 公开了使用电场进行改进的生物测定的方法和装置。 电场被施加在具有井的装置上，该装置具有承载电荷的反应物。 因此，该器件在第一和第二电极之间使用可控电压源，其可控制以向给定电极提供正电荷和负电荷。 通过控制使用流体通道中的流体中的电场带电物质通过电极之间的电场被引导进入或离开阱。 本方法涉及如在微流体装置中的流体的运输，以及根据各种测定程序（例如DNA测序，合成等）的反应物种的电场诱导运动。
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公开(公告)号：US08476022B2
公开(公告)日：2013-07-02
申请号：US13545682
申请日：2012-07-10
申请人： Mostafa Ronaghi , Helmy A. Eltoukhy
发明人： Mostafa Ronaghi , Helmy A. Eltoukhy
CPC分类号： C12Q1/6874 , C12Q1/6837 , C12Q1/6869 , C12Q2535/122 , C12Q2565/543
摘要： A method of making an array of nucleic acid colonies, by (a) providing a substrate having a patterned surface of features, wherein the features are spatially separated from each other on the surface of the substrate; (b) contacting the substrate with a solution of different target nucleic acids to seed a subset of the features that contact the solution, wherein each feature in the subset is seeded with a single nucleic acid from the solution, wherein a plurality of the features that contact the solution are not seeded with a nucleic acid from the solution; (c) amplifying the nucleic acids to form a nucleic acid colony at each of the features in the subset; and (d) repeating steps (b) and (c) to increase the number of the features on the surface that have a nucleic acid colony, thereby making an array of nucleic acid colonies.
摘要翻译： 通过（a）提供具有特征图案化表面的基底，制备核酸集落阵列的方法，其中所述特征在所述基底的表面上彼此空间上分离; （b）使所述底物与不同靶核酸的溶液接触以接种与所述溶液接触的特征的子集，其中所述亚组中的每个特征从所述溶液中接种单个核酸，其中所述多个特征 接触溶液不会从溶液中接种核酸; （c）扩增核酸以在子集中的每个特征上形成核酸集落; 和（d）重复步骤（b）和（c）以增加具有核酸集落的表面上的特征的数目，由此形成核酸集落阵列。
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公开(公告)号：US20120065091A1
公开(公告)日：2012-03-15
申请号：US13206120
申请日：2011-08-09
申请人： Thomas D. Willis , Paul Hardenbol , Maneesh Jain , Viktor Stolc , Mostafa Ronaghi , Ronald W. Davis
发明人： Thomas D. Willis , Paul Hardenbol , Maneesh Jain , Viktor Stolc , Mostafa Ronaghi , Ronald W. Davis
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q2531/113 , C12Q2525/307 , C12Q2537/143
摘要： The invention is directed to novel methods of multiplexing nucleic acid reactions, including amplification, detection and genotyping. The invention relies on the use of precircle probes that are circularized in the presence of the corresponding target nucleic acids, cleaved, and then amplified.
摘要翻译： 本发明涉及复制核酸反应的新方法，包括扩增，检测和基因分型。 本发明依赖于在相应靶核酸存在下被环化的前循环探针的使用，被切割，然后扩增。
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公开(公告)号：US20060008824A1
公开(公告)日：2006-01-12
申请号：US11134683
申请日：2005-05-19
申请人： Mostafa Ronaghi , Foad Mashayekhi
发明人： Mostafa Ronaghi , Foad Mashayekhi
CPC分类号： C12Q1/6874 , B01J2219/00497 , B01J2219/005 , B01J2219/00547 , B01J2219/00572 , B01J2219/00722 , C12Q2565/518 , C12Q2563/185
摘要： Described herein are methods and compositions relating to amplifying nucleic acid. In certain embodiments, the invention provides methods for labeling and amplifying a nucleic acid molecule.
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公开(公告)号：US08753816B2
公开(公告)日：2014-06-17
申请号：US13881320
申请日：2011-10-26
申请人： Roberto Rigatti , Jonathan Mark Boutell , Jason Richard Betley , Niall Anthony Gormley , Mostafa Ronaghi , Dirk Evers
发明人： Roberto Rigatti , Jonathan Mark Boutell , Jason Richard Betley , Niall Anthony Gormley , Mostafa Ronaghi , Dirk Evers
CPC分类号： C12Q1/6874 , C12Q2565/514 , C12Q2537/155 , C12Q2525/186 , C12Q2525/185 , C12Q2537/113 , C12Q2525/204
摘要： The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to nucleic acid sequencing.
摘要翻译： 本技术涉及分子科学，如基因组学。 更具体地，本技术涉及核酸测序。
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