公开(公告)号：US07521595B2

公开(公告)日：2009-04-21

申请号：US10995951

申请日：2004-11-23

申请人： Abdelali Hannoufa ,  Dwayne Hegedus ,  Nicholas Bate

发明人： Abdelali Hannoufa ,  Dwayne Hegedus ,  Nicholas Bate

IPC分类号： C12N15/82 ,  C12N15/63 ,  C12N15/31 ,  C12N15/00 ,  A01H5/10

CPC分类号： C12N15/8217 ,  C07K14/195

摘要： The invention provides a method for selectively controlling the transcription of a gene of interest, comprising producing one or more plants that express either a first, a second, or both the first and second genetic constructs. The first genetic construct comprises a first regulatory region operatively linked to a gene of interest and at least one repressor sequence capable of controlling the activity of the first regulatory region. The second genetic construct comprises a second regulatory region in operative association with a nucleic acid molecule, or a derivative thereof, encoding a repressor protein, the repressor protein exhibiting both repressor operator sequence binding activity and repressor activity.

摘要翻译： 本发明提供了选择性控制感兴趣的基因的转录的方法，其包括产生表达第一和第二遗传构建体的第一个，第二个或第二个的一个或多个植物。 第一遗传构建物包含与感兴趣的基因有效连接的第一调节区和至少一个能够控制第一调节区的活性的阻遏物序列。 第二遗传构建体包含与编码阻抑蛋白的核酸分子或其衍生物有效结合的第二调控区，所述阻遏蛋白具有阻遏物操纵子序列结合活性和阻遏物活性。

公开(公告)号：US07321077B2

公开(公告)日：2008-01-22

申请号：US10719996

申请日：2003-11-21

申请人： Abdelali Hannoufa ,  Dwayne Hegedus ,  Nicholas Bate

发明人： Abdelali Hannoufa ,  Dwayne Hegedus ,  Nicholas Bate

IPC分类号： C12N15/63 ,  C12N15/31 ,  C12N15/82

CPC分类号： C12N15/8217 ,  C07K14/195

摘要： The invention provides a method for selectively controlling the transcription of a gene of interest, comprising producing one or more plants that express either a first, a second, or both the first and second genetic constructs. The first genetic construct comprises a first regulator region operatively linked to a gene of interest and at least one repressor sequence capable of controlling the activity of the first regulatory region. The second genetic construct comprises a second regulator region in operative association with a nucleic acid molecule, or a derivative thereof, encoding a repressor protein, the repressor protein exhibiting both repressor operator sequence binding activity and repressor activity. The first and second genetic constructs may reside on separate vectors, or the vector may comprise both the first and second genetic constructs comprised as just defined. If the first and second constructs reside within separate plants, then the first plant and the second plant are crossed to obtain progeny, so that the progeny comprise both the first genetic construct and the second genetic construct. The progeny of this cross are characterized in that the expression of the second genetic construct represses expression of the gene of interest. The first and second regulatory regions may be either the same or different and may be selected from the group consisting of a constitutive promoter, an inducible promoter, a tissue specific promoter, and a developmental promoter. if the plant comprises the vector that comprises both the first and second genetic construct, or if a plant has been co-transformed with the first and second genetic construct so that both the first and second genetic constructs may be expressed in the same plant, then it is preferred that the first and second regulatory regions are different. The first regulatory region may comprise a constitutive promoter, an inducible promoter, a tissue specific promoter, or a developmental promoter. The second regulatory region may comprise an inducible promoter, a tissue specific promoter, or a developmental promoter.

公开(公告)号：US20050245732A1

公开(公告)日：2005-11-03

申请号：US10995951

申请日：2004-11-23

申请人： Abdelali Hannoufa ,  Dwayne Hegedus ,  Nicholas Bate

发明人： Abdelali Hannoufa ,  Dwayne Hegedus ,  Nicholas Bate

IPC分类号： C07K14/195 ,  C12N15/31 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C07H21/02

CPC分类号： C12N15/8217 ,  C07K14/195

摘要： The invention provides a method for selectively controlling the transcription of a gene of interest, comprising producing one or more plants that express either a first, a second, or both the first and second genetic constructs. The first genetic construct comprises a first regulatory region operatively linked to a gene of interest and at least one repressor sequence capable of controlling the activity of the first regulatory region. The second genetic construct comprises a second regulatory region in operative association with a nucleic acid molecule, or a derivative thereof, encoding a repressor protein, the repressor protein exhibiting both repressor operator sequence binding activity and repressor activity. The first and second genetic constructs may reside on separate vectors, or the vector may comprise both the first and second genetic constructs comprised as just defined. If the first and second constructs reside within separate plants, then the first plant and the second plant are crossed to obtain progeny, so that the progeny comprise both the first genetic construct and the second genetic construct. The progeny of this cross are characterized in that the expression of the second genetic construct represses expression of the gene of interest. The first and second regulatory regions may be either the same or different and may be selected from the group consisting of a constitutive promoter, an inducible promoter, a tissue specific promoter, and a developmental promoter. If the plant comprises the vector that comprises both the first and second genetic construct, or if a plant has been co-transformed with the first and second genetic construct so that both the first and second genetic constructs may be expressed in the same plant, then it is preferred that the first and second regulatory regions are different. The first regulatory region may comprise a constitutive promoter, an inducible promoter, a tissue specific promoter, or a developmental promoter. The second regulatory region may comprise an inducible promoter, a tissue specific promoter, or a developmental promoter.

公开(公告)号：US07560612B2

公开(公告)日：2009-07-14

申请号：US11527087

申请日：2006-09-26

申请人： Xiping Niu ,  Nicholas Bate

发明人： Xiping Niu ,  Nicholas Bate

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/823

摘要： The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for regulatory elements providing expression preferentially in the inflorescence meristem and developing floral tissues. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences are also provided, as well as expression constructs, vectors, and transformed cells and plants.

摘要翻译： 本发明提供用于调节植物中分离的核苷酸序列表达的组合物和方法。 组合物是用于调节元件的新型核酸序列，其优先在花序分生组织和发育中的花组织中提供表达。 还提供了使用调节序列在植物中表达分离的核苷酸序列的方法，以及表达构建体，载体和转化的细胞和植物。

公开(公告)号：US20070136891A1

公开(公告)日：2007-06-14

申请号：US11527087

申请日：2006-09-26

申请人： Xiping Niu ,  Nicholas Bate

发明人： Xiping Niu ,  Nicholas Bate

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/823

摘要： The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for regulatory elements providing expression preferentially in the inflorescence meristem and developing floral tissues. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences are also provided, as well as expression constructs, vectors, and transformed cells and plants.

摘要翻译： 本发明提供用于调节植物中分离的核苷酸序列表达的组合物和方法。 组合物是用于调节元件的新型核酸序列，其优先在花序分生组织和发育中的花组织中提供表达。 还提供了使用调节序列在植物中表达分离的核苷酸序列的方法，以及表达构建体，载体和转化的细胞和植物。

公开(公告)号：US20110016580A1

公开(公告)日：2011-01-20

申请号：US12428177

申请日：2009-04-22

申请人： Xiping Niu ,  Nicholas Bate

发明人： Xiping Niu ,  Nicholas Bate

IPC分类号： C12N15/82 ,  C07H21/04 ,  C12N15/63 ,  A01H5/00 ,  A01H5/10 ,  C12N5/10

CPC分类号： C12N15/8261 ,  C12N15/8222 ,  C12N15/8229 ,  Y02A40/146

摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. The compositions are novel nucleic acid sequences which confer cellular division and/or proliferation-preferred regulation of operably attached nucleotide sequences. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences, expression cassettes, vectors and resultant plants are also provided.

摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物是赋予可操作地连接的核苷酸序列的细胞分裂和/或增殖优选调节的新型核酸序列。 还提供了使用调节序列，表达盒，载体和所得植物在植物中表达分离的核苷酸序列的方法。

公开(公告)号：US20050120404A1

公开(公告)日：2005-06-02

申请号：US10388359

申请日：2003-03-13

申请人： Xiping Niu ,  Nicholas Bate

发明人： Xiping Niu ,  Nicholas Bate

IPC分类号： C12N15/82 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/22 ,  A01H1/00 ,  A01H5/00 ,  C12N5/04

CPC分类号： C12N15/8261 ,  C12N15/8222 ,  C12N15/8229 ,  Y02A40/146

摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. The compositions are novel nucleic acid sequences which confer cellular division and/or proliferation-preferred regulation of operably attached nucleotide sequences. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences, expression cassettes, vectors and resultant plants are also provided.

摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物是赋予可操作地连接的核苷酸序列的细胞分裂和/或增殖优选调节的新型核酸序列。 还提供了使用调节序列，表达盒，载体和所得植物在植物中表达分离的核苷酸序列的方法。

公开(公告)号：US20070238179A1

公开(公告)日：2007-10-11

申请号：US11751411

申请日：2007-05-21

申请人： Xiping Niu ,  Nicholas Bate

发明人： Xiping Niu ,  Nicholas Bate

IPC分类号： C12N15/00 ,  A01H5/00 ,  C12N15/82 ,  C12N5/00 ,  C07H21/04

CPC分类号： C12N15/8261 ,  C12N15/8222 ,  C12N15/8229 ,  Y02A40/146

摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. The compositions are novel nucleic acid sequences which confer cellular division and/or proliferation-preferred regulation of operably attached nucleotide sequences. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences, expression cassettes, vectors and resultant plants are also provided.

摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物是赋予可操作地连接的核苷酸序列的细胞分裂和/或增殖优选调节的新型核酸序列。 还提供了使用调节序列，表达盒，载体和所得植物在植物中表达分离的核苷酸序列的方法。