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公开(公告)号：US08144396B2
公开(公告)日：2012-03-27
申请号：US12060994
申请日：2008-04-02
IPC分类号： G02B21/26
CPC分类号： G02B21/33
摘要： An image can be prevented from becoming unclear over time during long-term observation. The invention provides a microscope apparatus including a specimen container for containing a specimen; an objective lens disposed opposite the specimen container for collecting light from the specimen in the specimen container; an immersion-liquid supplying unit for supplying immersion liquid to a space between the objective lens and the specimen container; and an immersion-liquid removing unit for removing the immersion liquid from the space between the objective lens and the specimen container. The immersion-liquid removing unit includes a nozzle for ejecting compressed air to the space between the objective lens and the specimen container.
摘要翻译： 在长期观察期间，可以防止图像变得不清楚。 本发明提供了一种显微镜装置，其包括：容纳样本的检体容器; 与样本容器相对设置的用于收集来自样本容器中的样本的光的物镜; 浸没液供给单元，其向所述物镜和所述检体容器之间的空间供给浸渍液; 以及用于从物镜和样本容器之间的空间中去除浸液的浸液清除单元。 浸液清除单元包括用于将压缩空气喷射到物镜和样本容器之间的空间的喷嘴。
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公开(公告)号：US07964853B2
公开(公告)日：2011-06-21
申请号：US12772361
申请日：2010-05-03
申请人： Akinori Araya
发明人： Akinori Araya
IPC分类号： G01N21/64
CPC分类号： G02B21/004 , G02B21/0076
摘要： A laser scanning microscope that can construct a fluorescence image with high resolution even with a photodetector having a small array size is provided. A laser scanning microscope includes a line-focusing optical system that focuses laser light from a laser light source in the form of a line; a DMD having a plurality of movable micromirrors that are arrayed in the same direction of the line and reflect the focused laser light; a galvanometer mirror that scans the laser light reflected by the DMD; an irradiation optical system that irradiates a sample with the scanned laser light; a photodetector having a plurality of channels that detect light from the sample and are arrayed in a single line; and a controller that controls the DMD so as to cause the light reflected by the different movable micromirrors to enter the respective channels, and that sequentially switches between the micromirrors that reflect the light that is to enter the channels so that the light from different positions on the sample enters the channels.
摘要翻译： 提供即使具有小阵列尺寸的光电检测器也可以构建高分辨率的荧光图像的激光扫描显微镜。 激光扫描显微镜包括以线形式聚焦来自激光光源的激光的线聚焦光学系统; 具有多个可移动微镜的DMD，其以相同方向排列并反射聚焦的激光; 用于扫描由DMD反射的激光的电流计镜; 照射光学系统，用扫描的激光照射样品; 具有多个通道的光电探测器，该多个通道检测来自样品的光并排列成一行; 以及控制器，其控制DMD以使得由不同的可移动微镜反射的光进入相应的通道，并且顺序地在反射进入通道的光的微反射镜之间切换，使得来自不同位置的光 样品进入通道。
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公开(公告)号：US20100282981A1
公开(公告)日：2010-11-11
申请号：US12772361
申请日：2010-05-03
申请人： Akinori ARAYA
发明人： Akinori ARAYA
CPC分类号： G02B21/004 , G02B21/0076
摘要： A laser scanning microscope that can construct a fluorescence image with high resolution even with a photodetector having a small array size is provided. A laser scanning microscope includes a line-focusing optical system that focuses laser light from a laser light source in the form of a line; a DMD having a plurality of movable micromirrors that are arrayed in the same direction of the line and reflect the focused laser light; a galvanometer mirror that scans the laser light reflected by the DMD; an irradiation optical system that irradiates a sample with the scanned laser light; a photodetector having a plurality of channels that detect light from the sample and are arrayed in a single line; and a controller that controls the DMD so as to cause the light reflected by the different movable micromirrors to enter the respective channels, and that sequentially switches between the micromirrors that reflect the light that is to enter the channels so that the light from different positions on the sample enters the channels.
摘要翻译： 提供即使具有小阵列尺寸的光电检测器也可以构建高分辨率的荧光图像的激光扫描显微镜。 激光扫描显微镜包括以线形式聚焦来自激光光源的激光的线聚焦光学系统; 具有多个可移动微镜的DMD，其以相同方向排列并反射聚焦的激光; 用于扫描由DMD反射的激光的电流计镜; 照射光学系统，用扫描的激光照射样品; 具有多个通道的光电探测器，该多个通道检测来自样品的光并排列成一行; 以及控制器，其控制DMD以使得由不同的可移动微镜反射的光进入相应的通道，并且顺序地在反射进入通道的光的微反射镜之间切换，使得来自不同位置的光 样品进入通道。
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公开(公告)号：US20090135476A1
公开(公告)日：2009-05-28
申请号：US12272923
申请日：2008-11-18
申请人： Akinori ARAYA
发明人： Akinori ARAYA
CPC分类号： G02B21/0032 , H01S5/0427 , H01S5/06832 , H01S5/06835
摘要： Beth of highly stable light having the same optical performance and fast-modulated light are emitted simply and inexpensively. A light source apparatus is provided, which includes semiconductor laser elements that emit laser light according to an inputted current signal, a light receiving element that receives the laser light emitted from the semiconductor laser element, and a controller that controls light emission of the semiconductor laser element, wherein the controller includes a first semiconductor laser element drive circuit that outputs a current signal to the semiconductor laser elements according to an instruction signal, a second semiconductor laser element drive circuit that adjusts the current signal on the basis of light quantity of the laser light received by the light receiving element, and outputs the adjusted current signal to the semiconductor laser element, and a circuit switching section that switches between the first and the second semiconductor laser element drive circuits according to an instruction signal.
摘要翻译： 具有相同光学性能和快速调制光的高度稳定的光的Beth被简单且廉价地发射。 提供了一种光源装置，其包括根据输入的电流信号发射激光的半导体激光元件，接收从半导体激光元件发射的激光的光接收元件和控制半导体激光器的发光的控制器 元件，其中所述控制器包括：第一半导体激光元件驱动电路，其根据指令信号向半导体激光元件输出电流信号;第二半导体激光元件驱动电路，其基于激光的光量调节电流信号 由光接收元件接收的光，并将经调整的电流信号输出到半导体激光元件;以及电路切换部，其根据指示信号切换第一和第二半导体激光元件驱动电路。
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公开(公告)号：US07369308B2
公开(公告)日：2008-05-06
申请号：US11050883
申请日：2005-02-04
IPC分类号： G02B21/06
CPC分类号： G01N21/648 , G01N21/6458 , G02B21/0088 , G02B21/16
摘要： A total internal reflection fluorescence microscope includes a light source which generates laser light having a plurality of wavelengths, a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination, a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination, an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen, and a controller which controls the incident angle adjuster such that the amount of the permeation depth of the evanescent light is the same when the wavelength of the laser light from the laser light source is switched.
摘要翻译： 全内反射荧光显微镜包括产生具有多个波长的激光的光源，通过物镜以预定的入射角将来自光源的激光照射在样本上并产生ev逝照明的聚焦光学系统 观察由于ev逝照明而产生的荧光的荧光观察单元，调整照射在样本上的激光的入射角的入射角度调节器，以及控制入射角度调节器的控制器， 当切换来自激光光源的激光的波长时，渐逝光的透过深度相同。
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公开(公告)号：US20070272885A1
公开(公告)日：2007-11-29
申请号：US11805874
申请日：2007-05-24
申请人： Yusuke Yamashita , Akinori Araya
发明人： Yusuke Yamashita , Akinori Araya
IPC分类号： G03B42/08
CPC分类号： G02B21/0076 , G02B21/0048 , G02B21/0064
摘要： To provide a laser scanning microscope including a laser beam source for stimulation that emits a laser beam for stimulation for applying photostimulation to a sample, a scanner that performs scanning with the laser beam for stimulation, a control device that controls the scanner, and an objective lens that condenses the laser beam for stimulation used for scanning by the scanner to the sample. The scanner has at least one acoustooptic device arranged on an optical path of the laser beam for stimulation. The control device determines a plurality of frequencies on the basis of the position and the range of a photostimulation region, and simultaneously applies the high-frequency signals of the determined plurality of frequencies to a vibrator attached to the acoustooptic device.
摘要翻译： 为了提供一种激光扫描显微镜，包括用于刺激的激光束源，其发射用于刺激的激光束以对样品施加光刺激，使用激光束进行刺激的扫描仪，控制扫描仪的控制装置和目标 将用于扫描器扫描的刺激的激光束聚集到样品上的透镜。 扫描仪具有至少一个设置在激光束的光路上用于刺激的声光装置。 控制装置基于光刺激区域的位置和范围来确定多个频率，并且将确定的多个频率的高频信号同时施加到附接到声光装置的振动器。
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公开(公告)号：US20050263690A1
公开(公告)日：2005-12-01
申请号：US11141517
申请日：2005-05-31
申请人： Akinori Araya , Makio Ueno
发明人： Akinori Araya , Makio Ueno
CPC分类号： G02B26/101 , G02B21/002 , G02F1/11 , G02F1/33
摘要： A laser scanning microscope includes an acousto-optic element or an electro-optic element in an illumination optical system which introduces a laser beam from a laser beam source of the laser scanning microscope into a microscope. The laser scanning microscope further includes a beam expander disposed between the laser beam source and the acousto-optic element or the electro-optic element.
摘要翻译： 激光扫描显微镜包括在激光扫描显微镜的激光束源将激光束引入显微镜的照明光学系统中的声光元件或电光元件。 激光扫描显微镜还包括设置在激光束源和声光元件或电光元件之间的光束扩展器。
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公开(公告)号：US07724426B2
公开(公告)日：2010-05-25
申请号：US11805874
申请日：2007-05-24
申请人： Yusuke Yamashita , Akinori Araya
发明人： Yusuke Yamashita , Akinori Araya
CPC分类号： G02B21/0076 , G02B21/0048 , G02B21/0064
摘要： To provide a laser scanning microscope including a laser beam source for stimulation that emits a laser beam for stimulation for applying photostimulation to a sample, a scanner that performs scanning with the laser beam for stimulation, a control device that controls the scanner, and an objective lens that condenses the laser beam for stimulation used for scanning by the scanner to the sample. The scanner has at least one acoustooptic device arranged on an optical path of the laser beam for stimulation. The control device determines a plurality of frequencies on the basis of the position and the range of a photostimulation region, and simultaneously applies the high-frequency signals of the determined plurality of frequencies to a vibrator attached to the acoustooptic device.
摘要翻译： 为了提供一种激光扫描显微镜，包括用于刺激的激光束源，其发射用于刺激的激光束以对样品施加光刺激，使用激光束进行刺激的扫描仪，控制扫描仪的控制装置和目标 将用于扫描器扫描的刺激的激光束聚集到样品上的透镜。 扫描仪具有至少一个设置在激光束的光路上用于刺激的声光装置。 控制装置基于光刺激区域的位置和范围来确定多个频率，并且将确定的多个频率的高频信号同时施加到附接到声光装置的振动器。
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公开(公告)号：US20080151366A1
公开(公告)日：2008-06-26
申请号：US12002508
申请日：2007-12-17
申请人： Akinori Araya , Tatsuo Nakata , Makio Ueno
发明人： Akinori Araya , Tatsuo Nakata , Makio Ueno
IPC分类号： G02B21/00
CPC分类号： G02B21/0036
摘要： It is possible to achieve a required field number and numerical aperture for microscope observation at a scanning speed equal to video-rate or higher and also to change the scanning speed with a simple configuration. The invention provides a laser microscope including a laser light source; a scanning unit configured to scan a specimen with laser light emitted from the laser light source; and an objective lens configured to focus the laser light scanned by the scanning unit on the specimen. The scanning unit is provided with an electro-optical deflecting element including an electro-optical crystal in which a refractive index gradient is induced by injecting electric current.
摘要翻译： 可以以等于视频速率或更高的扫描速度实现用于显微镜观察的所需场数和数值孔径，并且还可以以简单的配置改变扫描速度。 本发明提供一种包括激光光源的激光显微镜; 扫描单元，被配置为用从所述激光光源发射的激光扫描样本; 以及配置为将由扫描单元扫描的激光聚焦在样本上的物镜。 扫描单元设置有电光偏转元件，其包括通过注入电流而引起折射率梯度的电光晶体。
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公开(公告)号：US20050179903A1
公开(公告)日：2005-08-18
申请号：US11050883
申请日：2005-02-04
CPC分类号： G01N21/648 , G01N21/6458 , G02B21/0088 , G02B21/16
摘要： A total internal reflection fluorescence microscope includes a light source which generates laser light having a plurality of wavelengths, a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination, a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination, an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen, and a controller which controls the incident angle adjuster such that the amount of the permeation depth of the evanescent light is the same when the wavelength of the laser light from the laser light source is switched.
摘要翻译： 全内反射荧光显微镜包括产生具有多个波长的激光的光源，通过物镜以预定的入射角将来自光源的激光照射在样本上并产生ev逝照明的聚焦光学系统 观察由于ev逝照明而产生的荧光的荧光观察单元，调整照射在样本上的激光的入射角的入射角度调节器，以及控制入射角度调节器的控制器， 当切换来自激光光源的激光的波长时，渐逝光的透过深度相同。
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