公开(公告)号：US07141549B2

公开(公告)日：2006-11-28

申请号：US11054281

申请日：2005-02-08

申请人： Peter Mezes ,  Luca Rastelli ,  John Herrmann ,  John MacDougall ,  Haihong Zhong ,  Stacie Casman ,  Ferenc Boldog ,  Richard Shimkets ,  Linda Gorman ,  Andrew Eisen ,  Steven Spaderna ,  Corine Vernet ,  Constance Berghs ,  Kimberly Spytek ,  Vincent DiPippo ,  Bryan Zerhusen ,  John Peyman ,  Karen Ellerman ,  David Stone ,  William Grosse ,  John Alsobrook, II ,  Denise Lepley ,  Daniel Rieger ,  Catherine Burgess ,  Shlomit Edinger ,  Edward Voss ,  Charles Miller

发明人： Peter Mezes ,  Luca Rastelli ,  John Herrmann ,  John MacDougall ,  Haihong Zhong ,  Stacie Casman ,  Ferenc Boldog ,  Richard Shimkets ,  Linda Gorman ,  Andrew Eisen ,  Steven Spaderna ,  Corine Vernet ,  Constance Berghs ,  Kimberly Spytek ,  Vincent DiPippo ,  Bryan Zerhusen ,  John Peyman ,  Karen Ellerman ,  David Stone ,  William Grosse ,  John Alsobrook, II ,  Denise Lepley ,  Daniel Rieger ,  Catherine Burgess ,  Shlomit Edinger ,  Edward Voss ,  Charles Miller

IPC分类号： A61K38/17 ,  C07K14/47

CPC分类号： C12N9/2462 ,  A61K38/00 ,  A61K47/6829 ,  A61K47/6851 ,  A61K47/6869 ,  A61K2039/505 ,  C07K14/47 ,  C07K14/705 ,  C07K16/18 ,  G01N33/6893

摘要： Disclosed herein are nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.

公开(公告)号：US20060009634A1

公开(公告)日：2006-01-12

申请号：US11019711

申请日：2004-12-21

申请人： Ramesh Kekuda ,  John Alsobrook ,  Velizar Tchernev ,  Xiaohong Liu ,  Kimberly Spytek ,  Meera Patturajan ,  William Grosse ,  Denise Lepley ,  Catherine Burgess ,  Corine Vernet ,  Li Li ,  Linda Gorman ,  Shlomit Edinger ,  Paul Sciore ,  Karen Ellerman ,  Uriel Malyankar ,  Mark Rothenberg ,  David Stone ,  Ferenc Boldog ,  Xiaojia Guo ,  Suresh Shenoy ,  David Anderson ,  Muralidhara Padigaru ,  Raymond Taupier ,  Charles Miller ,  Andrew Eisen

发明人： Ramesh Kekuda ,  John Alsobrook ,  Velizar Tchernev ,  Xiaohong Liu ,  Kimberly Spytek ,  Meera Patturajan ,  William Grosse ,  Denise Lepley ,  Catherine Burgess ,  Corine Vernet ,  Li Li ,  Linda Gorman ,  Shlomit Edinger ,  Paul Sciore ,  Karen Ellerman ,  Uriel Malyankar ,  Mark Rothenberg ,  David Stone ,  Ferenc Boldog ,  Xiaojia Guo ,  Suresh Shenoy ,  David Anderson ,  Muralidhara Padigaru ,  Raymond Taupier ,  Charles Miller ,  Andrew Eisen

IPC分类号： C07H21/04

CPC分类号： C07K14/47

摘要： Disclosed herein are nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.

公开(公告)号：US06830910B1

公开(公告)日：2004-12-14

申请号：US10031893

申请日：2002-07-19

申请人： Andrew Eisen

发明人： Andrew Eisen

IPC分类号： C12N912

CPC分类号： C12N9/00 ,  A01K2217/05

摘要： The invention encompasses Drosophila Recombination Associated Protein (DRAP) isolated D. melanogaster and a nucleic acid sequence encoding DRAP. The Drosophila Recombination Associated Protein, its homologues from other organisms or active peptides derived therefrom, as well as DNA encoding such protein are useful for homology-dependent pairing of three DNA strands. The combination of strand-transfer and topoisomerase activities associated with DRAP permits directed pairing and cleavage at defined site(s) within DNA. This in turn makes possible the isolation and/or removal of a defined segment of DNA. DRAP is also useful in cloning, genomic cloning and gene mapping, in promoting gene disruptions or “knockout” mutations, in carrying out targeted mutagenesis of specific genes and in generating transgenic animals. The invention further encompasses a method for experimental and therapeutic application of DRAP driven knockouts or other modifications of genes responsible for genetic diseases as well as the use of DRAP driven genetic manipulation of genes in gene therapy.

摘要翻译： 本发明包括分离的黑腹果蝇的果蝇重组相关蛋白（DRAP）和编码DRAP的核酸序列。 果蝇重组相关蛋白，其来自其他生物的同源物或由其衍生的活性肽，以及编码这种蛋白质的DNA可用于三个DNA链的同源性依赖性配对。 与DRAP相关的链转移和拓扑异构酶活性的组合允许在DNA内的限定位点定向配对和切割。 这反过来使得能够分离和/或去除限定的DNA片段。 DRAP还可用于克隆，基因组克隆和基因作图，促进基因破坏或“敲除”突变，进行特异性基因的靶向诱变和产生转基因动物。 本发明进一步包括用于实验和治疗应用DRAP驱动敲除或负责遗传疾病的基因的其他修饰以及在基因治疗中使用DRAP驱动的基因遗传操作的方法。

公开(公告)号：US20050158751A1

公开(公告)日：2005-07-21

申请号：US11006372

申请日：2004-12-06

申请人： Andrew Eisen

发明人： Andrew Eisen

IPC分类号： C12N9/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N1/18 ,  C12N5/06 ,  C12N9/64

CPC分类号： C12N9/00 ,  A01K2217/05

摘要： The invention encompasses Drosophila Recombination Associated Protein (DRAP) isolated from D. melanogaster and a nucleic acid sequence encoding DRAP. The Drosophila Recombination Associated Protein, its homologues from other organisms or active peptides derived therefrom, as well as DNA encoding such protein are useful for homology-dependent pairing of three DNA strands. The combination of strand-transfer and topoisomerase activities associated with DRAP permits directed pairing and cleavage at defined site(s) within DNA. This in turn makes possible the isolation and/or removal of a defined segment of DNA. DRAP is also useful in cloning, genomic cloning and gene mapping, in promoting gene disruptions or “knockout” mutations, in carrying out targeted mutagenesis of specific genes and in generating transgenic animals. The invention further encompasses a method for experimental and therapeutic application of DRAP driven knockouts or other modifications of genes responsible for genetic diseases as well as the use of DRAP driven genetic manipulation of genes in gene therapy.

摘要翻译： 本发明包括从黑腹果蝇分离的果蝇重组相关蛋白（DRAP）和编码DRAP的核酸序列。 果蝇重组相关蛋白，其来自其他生物的同源物或由其衍生的活性肽，以及编码这种蛋白质的DNA可用于三个DNA链的同源性依赖性配对。 与DRAP相关的链转移和拓扑异构酶活性的组合允许在DNA内的限定位点定向配对和切割。 这反过来使得能够分离和/或去除限定的DNA片段。 DRAP还可用于克隆，基因组克隆和基因作图，促进基因破坏或“敲除”突变，进行特异性基因的靶向诱变和产生转基因动物。 本发明进一步包括用于实验和治疗应用DRAP驱动敲除或负责遗传疾病的基因的其他修饰以及在基因治疗中使用DRAP驱动的基因遗传操作的方法。

公开(公告)号：US06858716B2

公开(公告)日：2005-02-22

申请号：US10353174

申请日：2003-01-28

申请人： Andrew Eisen

发明人： Andrew Eisen

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/43 ,  A61K48/00 ,  A61P43/00 ,  C07K16/40 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10 ,  C12N9/90 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12N9/00 ,  A01K2217/05

摘要： The invention encompasses Drosophila Recombination Associated Protein (DRAP) isolated from D. melanogaster and a nucleic acid sequence encoding DRAP. The Drosophila Recombination Associated Protein, its homologues from other organisms or active peptides derived therefrom, as well as DNA encoding such protein are useful for homology-dependent pairing of three DNA strands. The combination of strand-transfer and topoisomerase activities associated with DRAP permits directed pairing and cleavage at defined site(s) within DNA. This in turn makes possible the isolation and/or removal of a defined segment of DNA. DRAP is also useful in cloning, genomic cloning and gene mapping, in promoting gene disruptions or “knockout” mutations, in carrying out targeted mutagenesis of specific genes and in generating transgenic animals. The invention further encompasses a method for experimental and therapeutic application of DRAP driven knockouts or other modifications of genes responsible for genetic diseases as well as the use of DRAP driven genetic manipulation of genes in gene therapy.

摘要翻译： 本发明包括从黑腹果蝇分离的果蝇重组相关蛋白（DRAP）和编码DRAP的核酸序列。 果蝇重组相关蛋白，其来自其他生物的同源物或由其衍生的活性肽，以及编码这种蛋白质的DNA可用于三个DNA链的同源性依赖性配对。 与DRAP相关的链转移和拓扑异构酶活性的组合允许在DNA内的限定位点定向配对和切割。 这反过来使得能够分离和/或去除限定的DNA片段。 DRAP还可用于克隆，基因组克隆和基因作图，促进基因破坏或“敲除”突变，进行特异性基因的靶向诱变和产生转基因动物。 本发明进一步包括用于实验和治疗应用DRAP驱动敲除或负责遗传疾病的基因的其他修饰以及在基因治疗中使用DRAP驱动的基因遗传操作的方法。

公开(公告)号：US06534643B1

公开(公告)日：2003-03-18

申请号：US09621377

申请日：2000-07-21

申请人： Andrew Eisen

发明人： Andrew Eisen

IPC分类号： C07H2104

CPC分类号： C12N9/00 ,  A01K2217/05

摘要： The invention encompasses Drosophila Recombination Associated Protein (DRAP) isolated from D. melanogaster and a nucleic acid sequence encoding DRAP. The Drosophila Recombination Associated Protein, its homologues from other organisms or active peptides derived therefrom, as well as DNA encoding such protein are useful for homology-dependent pairing of three DNA strands. The combination of strand-transfer and topoisomerase activities associated with DRAP permits directed pairing and cleavage at defined site(s) within DNA. This in turn makes possible the isolation and/or removal of a defined segment of DNA. DRAP is also useful in cloning, genomic cloning and gene mapping, in promoting gene disruptions or “knockout” mutations, in carrying out targeted mutagenesis of specific genes and in generating transgenic animals. The invention further encompasses a method for experimental and therapeutic application of DRAP driven knockouts or other modifications of genes responsible for genetic diseases as well as the use of DRAP driven genetic manipulation of genes in gene therapy.

摘要翻译： 本发明包括从黑腹果蝇分离的果蝇重组相关蛋白（DRAP）和编码DRAP的核酸序列。 果蝇重组相关蛋白，其来自其他生物的同源物或由其衍生的活性肽，以及编码这种蛋白质的DNA可用于三个DNA链的同源性依赖性配对。 与DRAP相关的链转移和拓扑异构酶活性的组合允许在DNA内的限定位点定向配对和切割。 这反过来使得能够分离和/或去除限定的DNA片段。 DRAP还可用于克隆，基因组克隆和基因作图，促进基因破坏或“敲除”突变，进行特异性基因的靶向诱变和产生转基因动物。 本发明进一步包括用于实验和治疗应用DRAP驱动敲除或负责遗传疾病的基因的其他修饰以及在基因治疗中使用DRAP驱动的基因遗传操作的方法。

公开(公告)号：US20060013813A1

公开(公告)日：2006-01-19

申请号：US11054281

申请日：2005-02-08

申请人： Peter Mezes ,  Luca Rastelli ,  John Herrmann ,  John MacDougall ,  Haihong Zhong ,  Stacie Casman ,  Frenc Boldog ,  Richard Shimkets ,  Linda Gorman ,  Andrew Eisen ,  Steven Spaderna ,  Corine Vernet ,  Constance Berghs ,  Kimberly Spytek ,  Vincent DiPippo ,  Bryan Zerhusen ,  John Peyman ,  Karen Ellerman ,  David Stone ,  William Grosse ,  John Alsobrook ,  Denise Lepley ,  Daniel Rieger ,  Catherine Burgess ,  Sholmit Edinger ,  Edward Voss ,  Charles Miller

发明人： Peter Mezes ,  Luca Rastelli ,  John Herrmann ,  John MacDougall ,  Haihong Zhong ,  Stacie Casman ,  Frenc Boldog ,  Richard Shimkets ,  Linda Gorman ,  Andrew Eisen ,  Steven Spaderna ,  Corine Vernet ,  Constance Berghs ,  Kimberly Spytek ,  Vincent DiPippo ,  Bryan Zerhusen ,  John Peyman ,  Karen Ellerman ,  David Stone ,  William Grosse ,  John Alsobrook ,  Denise Lepley ,  Daniel Rieger ,  Catherine Burgess ,  Sholmit Edinger ,  Edward Voss ,  Charles Miller

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  A61K39/395 ,  C07K14/705 ,  C07K16/18

CPC分类号： C12N9/2462 ,  A61K38/00 ,  A61K47/6829 ,  A61K47/6851 ,  A61K47/6869 ,  A61K2039/505 ,  C07K14/47 ,  C07K14/705 ,  C07K16/18 ,  G01N33/6893

摘要： Disclosed herein are nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.

公开(公告)号：US20050053930A1

公开(公告)日：2005-03-10

申请号：US10454246

申请日：2003-06-03

申请人： David Anderson ,  Ferenc Boldog ,  Catherine Burgess ,  Stacie Casman ,  Shlomit Edinger ,  Andrew Eisen ,  Karen Ellerman ,  Valerie Gerlach ,  Linda Gorman ,  Xiaojia Guo ,  Vladimir Gusev ,  Weizhen Ji ,  Li Li ,  John MacDougall ,  Uriel Malyankar ,  Isabelle Millet ,  Tatiana Ort ,  Muralidhara Padigaru ,  Sudhirdas Prayaga ,  Meera Patturajan ,  Carol Pena ,  John Peyman ,  Daniel Rieger ,  Mark Rothenberg ,  Paul Sciore ,  Suresh Shenoy ,  Glennda Smithson ,  Kimberly Spytek ,  David Stone ,  Raymond Taupier ,  Velizar Tchernev ,  Corine Vernet ,  Edward Voss ,  Bryan Zerhusen ,  Mei Zhong

发明人： David Anderson ,  Ferenc Boldog ,  Catherine Burgess ,  Stacie Casman ,  Shlomit Edinger ,  Andrew Eisen ,  Karen Ellerman ,  Valerie Gerlach ,  Linda Gorman ,  Xiaojia Guo ,  Vladimir Gusev ,  Weizhen Ji ,  Li Li ,  John MacDougall ,  Uriel Malyankar ,  Isabelle Millet ,  Tatiana Ort ,  Muralidhara Padigaru ,  Sudhirdas Prayaga ,  Meera Patturajan ,  Carol Pena ,  John Peyman ,  Daniel Rieger ,  Mark Rothenberg ,  Paul Sciore ,  Suresh Shenoy ,  Glennda Smithson ,  Kimberly Spytek ,  David Stone ,  Raymond Taupier ,  Velizar Tchernev ,  Corine Vernet ,  Edward Voss ,  Bryan Zerhusen ,  Mei Zhong

IPC分类号： A61K38/17 ,  C07H21/04 ,  C07K14/47 ,  C12Q1/68

CPC分类号： C07K14/47 ,  A61K38/00

摘要： Disclosed herein are nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies that immunospecifically bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the novel polypeptide, polynucleotide, or antibody specific to the polypeptide. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.

摘要翻译： 本文公开了编码新型多肽的核酸序列。 还公开了由这些核酸序列编码的多肽，以及免疫特异性结合多肽的抗体，以及多肽特异性的新型多肽，多核苷酸或抗体的衍生物，变体，突变体或片段。 本发明还公开了用于诊断，治疗和预防与这些新型人核酸和蛋白质中的任一种相关的病症的治疗，诊断和研究方法。