公开(公告)号：US20130305410A1

公开(公告)日：2013-11-14

申请号：US13843447

申请日：2013-03-15

申请人： Andrew Farmer Bent ,  Matthew Hudson ,  Brian Diers ,  Sara Melito ,  David Edward Cook ,  Teresa Hughes ,  Adam Bayless ,  Jianping Wang ,  Tong Geon Lee ,  Xiaoli Guo

发明人： Andrew Farmer Bent ,  Matthew Hudson ,  Brian Diers ,  Sara Melito ,  David Edward Cook ,  Teresa Hughes ,  Adam Bayless ,  Jianping Wang ,  Tong Geon Lee ,  Xiaoli Guo

IPC分类号： C12N15/82

CPC分类号： C12N15/8285 ,  A01H5/10 ,  C07K14/415 ,  Y02A40/164

摘要： Methods of increasing the resistance of plants, in particular soybeans, to nematodes, in particular soybean cyst nematodes, are provided herein. The methods include increasing the expression of Glyma18g02580, Glyma18g02590 and/or Glyma18g2610 in cells of a plant and in particular in root cells of a plant to increase the resistance of the plant and plant cells to nematodes. The methods include increasing the expression using constitutive promoters or by increasing the copy number of the polynucleotides. Constructs for expressing these polypeptides, transgenic cells, transgenic plants and methods of generating the same are also provided. Methods of screening plant cells for resistance or susceptibility to nematodes are also provided.

摘要翻译： 本文提供了将植物，特别是大豆，特别是大豆孢囊线虫的抗性增加的方法。 所述方法包括增加植物细胞中Glyma18g02580，Glyma18g02590和/或Glyma18g2610的表达，特别是在植物的根细胞中增加植物和植物细胞对线虫的抗性。 所述方法包括使用组成型启动子增加表达或增加多核苷酸的拷贝数。 还提供了用于表达这些多肽，转基因细胞，转基因植物和产生其的方法的构建体。 还提供了筛选植物细胞对线虫抗性或易感性的方法。

公开(公告)号：US20060019292A1

公开(公告)日：2006-01-26

申请号：US11181148

申请日：2005-07-13

申请人： Andrew Farmer

发明人： Andrew Farmer

IPC分类号： C40B40/08 ,  C12Q1/68 ,  C12N15/86 ,  C12N15/74

CPC分类号： C12N15/1093 ,  C12N15/1086 ,  C12N15/66

摘要： Methods are provided for producing a vector that includes at least one splicable intron. In the subject methods, intron containing vectors are produced from donor and acceptor vectors that each include a site specific recombinase site, where the subject donor and acceptor vectors further include splice donor and acceptor sites that, upon site specific recombination of the donor and acceptor vectors, define an intron in the product vector of the recombination step. Also provided are compositions for use in practicing the subject methods, including the donor and acceptor vectors themselves, as well as systems and kits that include the same. The subject invention finds use in a variety of different applications, including the production of expression vectors that encode C-terminal tagged fusion proteins, the production of expression vectors that encode pure protein and not a fusion thereof, and the like.

公开(公告)号：US20050060771A1

公开(公告)日：2005-03-17

申请号：US10927962

申请日：2004-08-27

申请人： Andrew Farmer

发明人： Andrew Farmer

IPC分类号： C12N15/11 ,  A01H1/00 ,  C12N15/82

CPC分类号： C12N15/111 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2330/30

摘要： siRNA encoding constructs and methods for using the same are provided. The subject constructs are characterized by including a siRNA coding domain flanked by opposing promoters. In using the subject constructs, sense and antisense strands of the desired siRNA product encoded by the coding domain are transcribed under the direction of the two opposing promoters flanking the coding domain. The transcribed sense and antisense strands are then annealed to each other to produce the desired siRNA double-stranded molecule. The subject constructs and methods find use in a variety of applications, including applications where the specific reduction or silencing of a gene is desired. Also provided are systems and kits for use in practicing the subject invention.

摘要翻译： 提供了siRNA编码构建体及其使用方法。 主题构建体的特征在于包括侧翼为相反启动子的siRNA编码区。 在使用受试者构建体时，由编码区编码的所需siRNA产物的有义链和反义链在编码区侧翼的两个相对的启动子的方向上转录。 然后将转录的有义链和反义链彼此退火以产生所需的siRNA双链分子。 主题构建体和方法可用于各种应用，包括需要特异性还原或沉默基因的应用。 还提供了用于实践本发明的系统和试剂盒。

公开(公告)号：US20180310497A1

公开(公告)日：2018-11-01

申请号：US15965113

申请日：2018-04-27

申请人： Jacob Andrew Farmer

发明人： Jacob Andrew Farmer

IPC分类号： A01G31/04 ,  A01G7/04 ,  A01G29/00

摘要： The present invention relates generally to agriculture. A growing system may use a movable cylinder which rotates around a central axis to secure various plants, where the root structure of the plant or plants is substantially contained inside a movable cylinder, and where plants and roots are watered or provided nutrients via a fluid which may be sprayed inside a movable cylinder, and where plants may grow their plant mass, stems, or harvestable parts substantially outside of movable cylinder. The various embodiments and methods of the present invention may allow for large quantities of plants to be grown via hydroponic or aeroponic techniques while taking up minimal space and may be used indoors.

公开(公告)号：US08162292B2

公开(公告)日：2012-04-24

申请号：US11587980

申请日：2005-04-28

申请人： Andrew Farmer

发明人： Andrew Farmer

IPC分类号： E04H17/00

CPC分类号： A01K3/00 ,  E04H17/003

摘要： An animal barrier device comprising one or more rotating bodies and fixture structures, in which in use the fixture structures, rotatably support the one or more rotating bodies on a construction with which the animal barrier is used, in which each rotating body is an elongate body which rotates in use about a longitudinal axis, and in which the longitudinal axis is located at any point on a line between a center of the rotating body and a point below the rotating body.

摘要翻译： 一种包括一个或多个旋转体和固定结构的动物屏障装置，其中在使用中，固定装置结构可旋转地将一个或多个旋转体支撑在使用动物屏障的结构上，其中每个旋转体是细长体 其在使用中围绕纵向轴线旋转，并且其中纵向轴线位于旋转体的中心和旋转体下方的点之间的线上的任何点处。

公开(公告)号：US20070141368A1

公开(公告)日：2007-06-21

申请号：US11306221

申请日：2005-12-20

申请人： Andrew Farmer ,  Bangalore Nagaraj ,  Wenfeng Lu ,  Ching-Pang Lee ,  James Guentert

发明人： Andrew Farmer ,  Bangalore Nagaraj ,  Wenfeng Lu ,  Ching-Pang Lee ,  James Guentert

IPC分类号： B05D3/12 ,  C23C4/08 ,  B32B15/04

CPC分类号： F01D5/288 ,  F01D9/041 ,  F05D2220/31 ,  F05D2230/21 ,  F05D2230/31 ,  F05D2230/90 ,  F05D2260/95 ,  F05D2300/15 ,  F05D2300/611

摘要： A gas turbine engine nozzle segment and process for producing such a nozzle segment to exhibit improved durability and aerodynamic performance. The process produces a nozzle segment having at least one vane between and interconnecting a pair of platforms. The nozzle segment is cast from a gamma prime-strengthened nickel-base superalloy, on whose surface is thermal sprayed an environmental coating formed of a MCrAlX-type coating material. The surface of the environmental coating is then worked to cause the coating to have a surface finish of less than 2.0 micrometers Ra. Cooling holes are then drilled in the nozzle segment, after which an oxidation-resistant coating is applied on the smoothed surface of the nozzle segment so as to maintain an outermost surface on the nozzle segment having surface finish of less than 2.0 micrometers Ra.

摘要翻译： 一种燃气涡轮发动机喷嘴段和用于生产这种喷嘴段的工艺，以显示出改善的耐久性和空气动力性能。 该过程产生具有在一对平台之间并互连的至少一个叶片的喷嘴段。 喷嘴段由伽马强化镍基超级合金铸造，其表面被热喷涂为由MCrAlX型涂层材料形成的环境涂层。 然后对环境涂层的表面进行加工，使涂层的表面光洁度小于2.0微米。 然后在喷嘴段中钻出冷却孔，然后在喷嘴段的平滑表面上施加耐氧化涂层，以便保持表面光洁度小于2.0微米Ra的喷嘴段上的最外表面。

公开(公告)号：US20060093849A1

公开(公告)日：2006-05-04

申请号：US10978427

申请日：2004-11-02

申请人： Andrew Farmer ,  Bangalore Nagaraj ,  Roger Wustman ,  Theodore Grossman ,  John Reiss

发明人： Andrew Farmer ,  Bangalore Nagaraj ,  Roger Wustman ,  Theodore Grossman ,  John Reiss

IPC分类号： B05D3/02

CPC分类号： C23C30/00 ,  C23C10/28 ,  C23C10/56 ,  C23C28/023 ,  C23C28/028 ,  Y10T428/12743 ,  Y10T428/12847 ,  Y10T428/12875

摘要： A method for applying a chromium containing coating to an underlying metal substrate where the metal substrate has an overlaying platinum-containing layer, as well as a corrosion resistant coated article thereof. A chromium-containing layer is deposited on the platinum-containing layer with an aluminide diffusion layer being deposited on the chromium-containing layer, the aluminide diffusion layer having an inner diffusion layer adjacent the chromium-containing layer and an outer additive layer adjacent to the inner diffusion layer. The chromium-containing layer is deposited by a deposition technique that permits chromium in the chromium-containing layer to more readily diffuse into a subsequently deposited aluminde diffusion coating layer. The chromium-containing and aluminide diffusion layers are then treated to cause chromium from the chromium-containing layer to diffuse into the outer additive layer in an amount of at least about 8%. The resulting coated article is resistant to corrosion.

摘要翻译： 一种将含铬涂层施加到金属基底上的金属基底具有覆盖的含铂层的方法，以及其耐腐蚀涂层制品。 含铬层沉积在含铂层上，铝化物扩散层沉积在含铬层上，铝化物扩散层具有邻近含铬层的内部扩散层和邻近含铬层的外部添加剂层 内扩散层。 通过沉积技术沉积含铬层，其允许含铬层中的铬更容易扩散到随后沉积的铝粘合扩散涂层中。 然后对含铬和铝化物扩散层进行处理，使含铬层的铬以至少约8％的量扩散到外部添加剂层中。 所得涂层制品耐腐蚀。

公开(公告)号：US07927807B1

公开(公告)日：2011-04-19

申请号：US12705151

申请日：2010-02-12

申请人： David P Fries ,  John H Paul ,  Andrew Farmer ,  Matthew Smith

发明人： David P Fries ,  John H Paul ,  Andrew Farmer ,  Matthew Smith

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12M1/00

CPC分类号： G01N1/02 ,  G01N35/00 ,  G01N35/00009 ,  G01N35/00069 ,  G01N2035/00881

摘要： An autonomous genosensor apparatus and methods for use are provided for the field detection and analysis of ambient chemical, biochemical, biologic, biogenetic, and radiologic materials under field conditions in fluid or gaseous environments, such as marine or aquatic environments or industrial processes. Autonomous genosensors provide integral, self contained units which automatically extract environmental samples, prepare those samples for analytical studies, analyze those samples using studies such as DNA or biomarker analysis, and store or transmit the data produced to a remote computer or computer network. Autonomous genosensors may be used as freestanding units, or may be networked and controlled through a remote computer network.

摘要翻译： 提供了一种自主的传感器装置和使用方法，用于在流体或气体环境（如海洋或水生环境或工业过程）的现场条件下对环境化学，生化，生物，生物和放射性材料的现场检测和分析。 自主基因传感器提供一体化的自包含单元，可自动提取环境样品，准备样品进行分析研究，使用DNA或生物标志物分析等研究分析样品，并将产生的数据存储或传输到远程计算机或计算机网络。 自主基因传感器可以用作独立单元，或者可以通过远程计算机网络联网和控制。

公开(公告)号：US07186989B2

公开(公告)日：2007-03-06

申请号：US10908994

申请日：2005-06-03

申请人： Andrew Farmer ,  David P. Fries ,  Bill Flanery

发明人： Andrew Farmer ,  David P. Fries ,  Bill Flanery

IPC分类号： G01J1/58

CPC分类号： G01N21/645 ,  G01N21/0332 ,  G01N2201/0221

摘要： The present invention provides core technologies necessary for a portable, low cost, thermal-regulating LED-based handheld fluorometer. The regulated fluorometer is based on a low thermal mass infrared heater, and an orthogonal geometry LED based filter fluorometer. Power is supplied through an external power supply and data is collected in real-time through standard serial interfaces of personal computers or personal digital assistants. Thermal regulation is automatically maintained using temperature sensor feedback control. Optical excitation relies on LED light source(s) and optical detection is through an adjustable integrating photodetector. Such a handheld system can allow applications requiring temperature sensitive photometric measurements for real time analyte detection to be performed in the field.

摘要翻译： 本发明提供了便携式，低成本，基于热调节的基于LED的手持式荧光计所需的核心技术。 调节荧光计是基于低热量红外加热器和基于正交几何LED的滤光片。 通过外部电源提供电源，并通过个人电脑或个人数字助理的标准串行接口实时收集数据。 使用温度传感器反馈控制自动保持热调节。 光学激发依赖于LED光源，光学检测是通过可调整的积分光电检测器。 这样的手持系统可以允许在现场执行需要温度敏感测光测量的实时实时分析物检测的应用。

公开(公告)号：US20200271952A1

公开(公告)日：2020-08-27

申请号：US16757014

申请日：2018-10-15

申请人： Andrew Farmer

发明人： Andrew Farmer

IPC分类号： G02C5/14

摘要： An eyewear device having a frame positionable to a removable engagement with any of a plurality of lens assemblies. Each lens assembly has shoulders at opposing ends which slidably engage within respective shoulder slots formed into the body of the frame. A biased ball having a portion extending into one or both shoulder slots, biasly contacts into a ball cavity depending into a respective shoulder to maintain the lens assembly in the removable engagement.