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1.发明申请Plants Having Increased Oil, Oleic Acid Content and Digestibility and Methods of Producing Same 审中-公开增加油，油酸含量和消化率的植物及其生产方法公开(公告)号：US20110126319A1
公开(公告)日：2011-05-26
申请号：US12945530
申请日：2010-11-12
申请人： Sally A. Catron , Chun-Hsiang Chang , Kimberly F. Glassman , Todd Leister , Mitchell C. Tarczynski
发明人： Sally A. Catron , Chun-Hsiang Chang , Kimberly F. Glassman , Todd Leister , Mitchell C. Tarczynski
CPC分类号： C12N15/8247
摘要： The present invention is directed to compositions and methods for producing corn plants and grain having increased oil content, increased oleic acid content of the oil, and increased digestibility over commodity corn grain. The resulting grain finds use in agricultural and industrial applications.
摘要翻译： 本发明涉及用于生产具有增加的油含量，增加的油的油酸含量以及与商品玉米粒的消化率增加的玉米植物和谷物的组合物和方法。 所得谷物用于农业和工业应用。
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2.发明申请LEAFY COTYLEDON 1 TRANSCRIPTIONAL ACTIVATOR (LEC1) VARIANT POLYNUCLEOTIDES AND POLYPEPTIDES COMPOSITIONS AND METHODS OF INCREASING TRANSFORMATION EFFICIENCY 审中-公开LEAFY COTYLEDON 1转录激活剂（LEC1）变体多核苷酸和多肽组合物和提高转化效率的方法公开(公告)号：US20110099665A1
公开(公告)日：2011-04-28
申请号：US12650259
申请日：2009-12-30
申请人： BO SHEN , MITCHELL C. TARCZYNSKI , IOWA STATE BANK , CHANGJIANG LI , KIMBERLY F. GLASSMAN , ARAGULA GURURAJ RAO , WILLIAM J. GORDON-KAMM
发明人： BO SHEN , MITCHELL C. TARCZYNSKI , IOWA STATE BANK , CHANGJIANG LI , KIMBERLY F. GLASSMAN , ARAGULA GURURAJ RAO , WILLIAM J. GORDON-KAMM
CPC分类号： C07K14/415 , C12N15/8216
摘要： The present invention provides LEC1 variants. The LEC1 variants comprise a LEC1 A domain, a LEC1 B domain and a LEC1 C domain, where the LEC1 B domain has at least one mutation and/or is chimeric with respect to the LEC1 A or C domain. The invention also includes methods of preparing such LEC1 variants, and methods of using such LEC1 variants to modulate the level or activity of LEC1 variants in a plant cell. Modulation of LEC1 activity or levels can be used for different purposes such as increasing transformation efficiency, stimulating growth of somatic embryos, improving the growth and recovery of transformants, inducing apomixes, increasing transformation frequency, enhancing tissue culture response and the like.
摘要翻译： 本发明提供了LEC1变体。 LEC1变体包含LEC1A结构域，LEC1B结构域和LEC1C结构域，其中LEC1B结构域具有至少一个突变和/或相对于LEC1A或C结构域是嵌合的。 本发明还包括制备这种LEC1变体的方法，以及使用这样的LEC1变体来调节植物细胞中LEC1变体的水平或活性的方法。 LEC1活性或水平的调节可以用于不同的目的，例如增加转化效率，刺激体细胞胚的生长，改善转化体的生长和恢复，诱导无融合体，增加转化频率，增强组织培养反应等。
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公开(公告)号：US07897383B2
公开(公告)日：2011-03-01
申请号：US12256721
申请日：2008-10-23
申请人： Anthony J. Kinney , Kimberly F. Glassman , William James Gordon-Kamm , Keith S. Lowe , Scott E. Nichols , Kevin L. Stecca
发明人： Anthony J. Kinney , Kimberly F. Glassman , William James Gordon-Kamm , Keith S. Lowe , Scott E. Nichols , Kevin L. Stecca
CPC分类号： C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/825 , C12N15/8251
摘要： Recombinant constructs useful for reducing the expression of endogenous mRNA and any substantially similar endogenous mRNA are disclosed. In particular, a recombinant construct comprising, inter alia, a suitable nucleic acid sequence and its reverse complement can be used to alter the expression of any homologous, endogenous RNA (i.e., the target RNA) which is in proximity to this suitable nucleic acid sequence.
摘要翻译： 公开了可用于减少内源性mRNA的表达和任何基本上相似的内源mRNA的重组构建体。 特别地，包含尤其是合适的核酸序列及其反向互补序列的重组构建体可用于改变任何同源的内源RNA（即靶RNA）的表达，其接近该合适的核酸序列 。
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4.发明申请LEAFY COTYLEDON 1 TRANSCRIPTIONAL ACTIVATOR (LEC1) VARIANT POLYNUCLEOTIDES AND POLYPEPTIDES, COMPOSITIONS AND METHODS OF INCREASING OIL CONTENT IN PLANTS 审中-公开LEAFY COTYLEDON 1转录激活剂（LEC1）变种多糖和多糖，组合物和增加植物油含量的方法公开(公告)号：US20100319086A1
公开(公告)日：2010-12-16
申请号：US12650188
申请日：2009-12-30
申请人： BO SHEN , MITCHELL C. TARCZYNSKI , CHANGJIANG LI , KIMBERLY F. GLASSMAN , ARAGULA GURURAJ RAO , WUAS SHAANALEYZ
发明人： BO SHEN , MITCHELL C. TARCZYNSKI , CHANGJIANG LI , KIMBERLY F. GLASSMAN , ARAGULA GURURAJ RAO , WUAS SHAANALEYZ
CPC分类号： C07K14/415 , C12N15/8247
摘要： The present invention provides LEC1 variants. The LEC1 variants comprise a LEC1 A domain, a LEC1 B domain and a LEC1 C domain, where the LEC1 B domain has at least one mutation and/or is chimeric with respect to the LEC1 A or C domain. The invention also includes methods of preparing such LEC 1 variants, and methods of using such LEC1 variants to modulate the level or activity of LEC1 variants in a plant cell. Modulation of LEC1 activity or levels can be used for different purposes such as increasing embryo oil concentration, oil content of a seed or altering oil phenotype in a plant.
摘要翻译： 本发明提供了LEC1变体。 LEC1变体包含LEC1A结构域，LEC1B结构域和LEC1C结构域，其中LEC1B结构域具有至少一个突变和/或相对于LEC1A或C结构域是嵌合的。 本发明还包括制备这种LEC1变体的方法，以及使用这种LEC1变体调节植物细胞中LEC1变体的水平或活性的方法。 LEC1活性或水平的调节可以用于不同的目的，例如增加植物油中的胚胎油浓度，种子油含量或改变油表型。
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5.发明申请Polynucleotides Encoding Stop Codons in Multiple Reading Frames and Methods of Use 失效多核苷酸编码多个阅读框中的停止密码子的多核苷酸和使用方法公开(公告)号：US20090038034A1
公开(公告)日：2009-02-05
申请号：US12183135
申请日：2008-07-31
CPC分类号： C12N15/8218
摘要： Compositions having polynucleotides encoding multiple translational stop signals in more than one reading frame are provided. The compositions include isolated polynucleotides, expression cassettes, and vectors, as well as host cells, prokaryotic organisms, and eukaryotic organisms comprising the polynucleotide(s). Methods include using the polynucleotides to stop translation of an mRNA into a protein, to produce a transformed cell and/or organism comprising the polynucleotide, and to identify transformed cells or organisms of a specific lineage.
摘要翻译： 提供了在多于一个阅读框中具有编码多个平移停止信号的多核苷酸的组合物。 组合物包括分离的多核苷酸，表达盒和载体，以及宿主细胞，原核生物和包含多核苷酸的真核生物。 方法包括使用多核苷酸停止将mRNA翻译成蛋白质，以产生包含该多核苷酸的转化细胞和/或生物体，并鉴定特定谱系的转化细胞或生物体。
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公开(公告)号：US20070261136A1
公开(公告)日：2007-11-08
申请号：US11741821
申请日：2007-04-30
CPC分类号： C12N15/8245 , C12N9/1051
摘要： The present invention is directed to compositions and methods for producing a dominant high-amylopectin starch trait in cereal grain and methods for screening grain for genetic variation in hydrolysis and fermentation time.
摘要翻译： 本发明涉及用于生产谷物中主要的高支链淀粉性状的组合物和方法以及用于筛选水解和发酵时间遗传变异的谷物的方法。
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7.发明申请NUCLEOTIDES ENCODING STOP CODONS IN MULTIPLE READING FRAMES AND METHODS OF USE 审中-公开在多个读取框架中编码停止编码的核心和使用方法公开(公告)号：US20120164734A1
公开(公告)日：2012-06-28
申请号：US13413850
申请日：2012-03-07
CPC分类号： C12N15/8218
摘要： Compositions having polynucleotides encoding multiple translational stop signals in more than one reading frame are provided. The compositions include isolated polynucleotides, expression cassettes, and vectors, as well as host cells, prokaryotic organisms, and eukaryotic organisms comprising the polynucleotide(s). Methods include using the polynucleotides to stop translation of an mRNA into a protein, to produce a transformed cell and/or organism comprising the polynucleotide, and to identify transformed cells or organisms of a specific lineage.
摘要翻译： 提供了在多于一个阅读框中具有编码多个平移停止信号的多核苷酸的组合物。 组合物包括分离的多核苷酸，表达盒和载体，以及宿主细胞，原核生物和包含多核苷酸的真核生物。 方法包括使用多核苷酸停止将mRNA翻译成蛋白质，以产生包含该多核苷酸的转化细胞和/或生物体，并鉴定特定谱系的转化细胞或生物体。
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公开(公告)号：US20090142829A1
公开(公告)日：2009-06-04
申请号：US12256721
申请日：2008-10-23
申请人： Kimberly F. Glassman , William J. Gordon-Kamm , Anthony J. Kinney , Keith S. Lowe , Scott E. Nichols , Kevin L. Stecca
发明人： Kimberly F. Glassman , William J. Gordon-Kamm , Anthony J. Kinney , Keith S. Lowe , Scott E. Nichols , Kevin L. Stecca
CPC分类号： C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/825 , C12N15/8251
摘要： Recombinant constructs useful for reducing the expression of endogenous mRNA and any substantially similar endogenous mRNA are disclosed. In particular, a recombinant construct comprising, inter alia, a suitable nucleic acid sequence and its reverse complement can be used to alter the expression of any homologous, endogenous RNA (i.e., the target RNA) which is in proximity to this suitable nucleic acid sequence.
摘要翻译： 公开了可用于减少内源性mRNA的表达和任何基本上相似的内源mRNA的重组构建体。 特别地，包含尤其是合适的核酸序列及其反向互补序列的重组构建体可用于改变任何同源的内源RNA（即靶RNA）的表达，其接近该合适的核酸序列 。
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公开(公告)号：US5258300A
公开(公告)日：1993-11-02
申请号：US204388
申请日：1988-06-09
IPC分类号： A01H5/00 , A01H1/02 , A01H4/00 , C07K14/415 , C12N5/10 , C12N9/88 , C12N15/09 , C12N15/82 , C12P13/08 , C12R1/91 , C12N5/14 , C12N15/32
CPC分类号： C07K14/415 , A01H1/02 , C12N15/8254 , C12N9/88
摘要： A method is provided for increasing the level of free L-lysine in a plant comprising: (a) introducing a foreign gene into the cells of a plant tissue source; and (b) expressing said foreign gene in said cells, wherein a first DNA sequence of said gene encodes dihydrodipicolinic acid synthase (DHDPS) which is substantially resistant to feedback inhibition by endogenously-produced free L-lysine.
摘要翻译： 提供了一种用于增加植物中游离L-赖氨酸水平的方法，包括：（a）将外源基因导入植物组织来源的细胞; 和（b）在所述细胞中表达所述外源基因，其中所述基因的第一DNA序列编码二氢吡啶二羧酸合成酶（DHDPS），其基本上抗内源产生的游离L-赖氨酸的反馈抑制。
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