公开(公告)号：US09233862B2

公开(公告)日：2016-01-12

申请号：US14131323

申请日：2012-07-10

申请人： Arijit Bose ,  Vijay T. John ,  Ani Tzankova Nikova

发明人： Arijit Bose ,  Vijay T. John ,  Ani Tzankova Nikova

IPC分类号： C02F1/68 ,  C09K3/32 ,  C02F1/28 ,  C02F3/00 ,  C02F3/34

CPC分类号： C02F1/68 ,  C02F1/281 ,  C02F1/681 ,  C02F3/00 ,  C02F3/343 ,  C09K3/32

摘要： A method for cleaning an oil spill in a marine environment includes forming a particle-stabilized emulsion containing seawater, carbon black and at least one oil spill component and allowing the at least one oil spill component to degrade, thereby removing said component from the marine environment. Carbon black can be added to an oil-seawater mixture to form a stabilized emulsion containing at least one oil spill component and the oil spill component allowed to degrade, thereby removing the at least one oil spill component from the oil spill. Also disclosed is an emulsion that includes one or more oil spill components, seawater and carbon black particles.

摘要翻译： 用于清洁海洋环境中的溢油的方法包括形成包含海水，炭黑和至少一种溢油成分的颗粒稳定的乳液，并允许至少一种溢油组分降解，从而从海洋环境中除去所述组分 。 可以将炭黑加入到油 - 海水混合物中以形成含有至少一种溢油组分和允许降解的溢油组分的稳定化乳液，从而从溢油中除去至少一种溢油组分。 还公开了包含一种或多种溢油组分，海水和炭黑颗粒的乳液。

公开(公告)号：US07816141B2

公开(公告)日：2010-10-19

申请号：US11338566

申请日：2006-01-24

申请人： Arijit Bose

发明人： Arijit Bose

IPC分类号： G01N33/00

CPC分类号： G01N1/42 ,  G01N1/2813

摘要： A modified freeze direct imaging of a viscous surfactant mesophase method. A chamber is provided having controlled temperature and solvent partial pressure. The chamber has two copper planchettes at the top and bottom thereof. A sample is placed in the chamber on a grid and is squeezed between the planchettes into a thin film. The thin film is placed in a liquid to vitrify the sample. The sample is removed from the planchettes to fracture the sample. The sample is then placed on a cold stage; and imaged.

摘要翻译： 粘性表面活性剂中间相方法的改进冷冻直接成像。 提供具有受控温度和溶剂分压的室。 该室在其顶部和底部具有两个铜制平面。 将样品放置在格栅上的腔室中，并在平面片之间挤压成薄膜。 将薄膜放置在液体中以使样品玻璃化。 从样品中取出样品以破碎样品。 然后将样品置于冷平台上; 并成像。

公开(公告)号：US07507533B2

公开(公告)日：2009-03-24

申请号：US11232597

申请日：2005-09-22

申请人： Arijit Bose ,  Nazneen Aziz

发明人： Arijit Bose ,  Nazneen Aziz

IPC分类号： C12Q1/00

CPC分类号： G01N1/42 ,  B01L3/022 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/68 ,  G01N2001/2873 ,  H01J2237/2001 ,  H01J2237/204

摘要： A system for generating an image of ultrastructural biomarkers from a biological sample is provided. The system includes a grid onto which a sample to be imaged may be placed and a cryogenic reservoir into which the grid and sample may be immersed for vitrification of the sample. The system also includes a stage onto which the grid and sample may be situated for subsequent imaging in a high contrast imager to permit identification of ultrastructural biomarkers therein. A method for generating an image of ultrastructural biomarkers from a biological sample is also provided. The generated image of ultrastructural biomarkers may be used subsequently for screening and monitoring diseases, evaluating drug and therapeutic efficacy, and assessing risks associated with a drug or therapeutic candidate, among other things.

摘要翻译： 提供了一种用于从生物样品生成超微结构生物标志物的图像的系统。 该系统包括可以放置要成像的样品的栅格和可以将栅格和样品浸入其中的低温储存器，用于玻璃化样品。 该系统还包括一个阶段，栅格和样本可以位于该阶段，用于在高对比度成像仪中随后成像，以允许在其中识别超微结构生物标志物。 还提供了用于从生物样品生成超微结构生物标志物的图像的方法。 生成的超微结构生物标志物的图像可以随后用于筛选和监测疾病，评估药物和治疗功效，以及评估与药物或治疗候选物相关的风险等。

公开(公告)号：US09355813B2

公开(公告)日：2016-05-31

申请号：US13439296

申请日：2012-04-04

申请人： Arijit Bose ,  Anubhav Tripathi ,  Jinkee Lee

发明人： Arijit Bose ,  Anubhav Tripathi ,  Jinkee Lee

IPC分类号： H01J37/20

CPC分类号： H01J37/20 ,  H01J2237/2001 ,  H01J2237/2002 ,  H01J2237/208 ,  H01J2237/2802

摘要： A method and system is provided for automatically preparing transmission electron microscopy (TEM) samples for examination by depositing extremely small samples onto a grid without need for a blotting step. A sample liquid droplet is formed at the end of a capillary, wherein a portion of the liquid is transferred to the TEM sample grid by contact. The excess volume in the liquid droplet is then retracted by an adjacent capillary. After a predetermined time interval, the retraction capillary is moved toward the drop of the sample to remove the excess volume. As compared to a conventional machine, where the blotting procedure can deform the structure of the molecule of interest, the present invention utilizes a very low shear rate for removal of the excess sample fluid.

摘要翻译： 提供了一种方法和系统，用于通过将非常小的样品沉积到网格上而无需印迹步骤，自动制备透射电子显微镜（TEM）样品进行检查。 在毛细管末端形成一个样品液滴，其中一部分液体通过接触转移到TEM样品网格。 液滴中的过剩体积然后被相邻的毛细管缩回。 在预定时间间隔之后，使收缩毛细管朝向样品的液滴移动以除去过量的体积。 与常规机器相比，其中印迹方法可能使感兴趣的分子的结构变形，本发明利用非常低的剪切速率去除多余的样品流体。

公开(公告)号：US20120241607A1

公开(公告)日：2012-09-27

申请号：US13439296

申请日：2012-04-04

申请人： Arijit Bose ,  Anubhav Tripathi ,  Jinkee Lee

发明人： Arijit Bose ,  Anubhav Tripathi ,  Jinkee Lee

IPC分类号： H01J37/20

CPC分类号： H01J37/20 ,  H01J2237/2001 ,  H01J2237/2002 ,  H01J2237/208 ,  H01J2237/2802

摘要： A method and system is provided for automatically preparing transmission electron microscopy (TEM) samples for examination by depositing extremely small samples onto a grid without need for a blotting step. A sample liquid droplet is formed at the end of a capillary, wherein a portion of the liquid is transferred to the TEM sample grid by contact. The excess volume in the liquid droplet is then retracted by an adjacent capillary. After a predetermined time interval, the retraction capillary is moved toward the drop of the sample to remove the excess volume. As compared to a conventional machine, where the blotting procedure can deform the structure of the molecule of interest, the present invention utilizes a very low shear rate for removal of the excess sample fluid.

摘要翻译： 提供了一种方法和系统，用于通过将非常小的样品沉积到网格上而无需印迹步骤，自动制备透射电子显微镜（TEM）样品进行检查。 在毛细管末端形成一个样品液滴，其中一部分液体通过接触转移到TEM样品网格。 液滴中的过剩体积然后被相邻的毛细管缩回。 在预定时间间隔之后，使收缩毛细管朝向样品的液滴移动以除去过量的体积。 与常规机器相比，其中印迹方法可能使感兴趣的分子的结构变形，本发明利用非常低的剪切速率去除多余的样品流体。

公开(公告)号：US20100216247A1

公开(公告)日：2010-08-26

申请号：US12622291

申请日：2009-11-19

申请人： Arijit Bose ,  Nazneen Aziz ,  Ashish Jha

发明人： Arijit Bose ,  Nazneen Aziz ,  Ashish Jha

IPC分类号： G01N33/48

CPC分类号： G01N33/57434

摘要： A system and method for determining presence of prostate cancer or prostate related diseases is provided. The method includes first obtaining a test sample from a test subject. Next, at least one ultrastructural biomarker indicative of prostate cancer within the sample may be identified. Thereafter, qualitative or quantitative data from the ultrastructural biomarker in the test sample can be obtained. The qualitative or quantitative data of the biomarker in the sample from the test subject can then be compared to that in a sample from a control subject for variations. The presence of qualitative and quantitative variations can act as a determinant for prostate cancer or prostate related diseases.

摘要翻译： 提供了一种用于确定前列腺癌或前列腺相关疾病的存在的系统和方法。 该方法包括首先从测试对象获得测试样本。 接下来，可以鉴定至少一个指示样品中前列腺癌的超微结构生物标志物。 此后，可以获得来自测试样品中的超微结构生物标志物的定性或定量数据。 然后可以将来自测试对象的样品中生物标志物的定性或定量数据与来自对照受试者的样品进行比较。 定性和定量变异的存在可以作为前列腺癌或前列腺相关疾病的决定因素。

公开(公告)号：US20060177935A1

公开(公告)日：2006-08-10

申请号：US11338566

申请日：2006-01-24

申请人： Arijit Bose

发明人： Arijit Bose

IPC分类号： G01N33/00

CPC分类号： G01N1/42 ,  G01N1/2813

摘要： A modified freeze direct imaging of a viscous surfactant mesophase method. A chamber is provided having controlled temperature and solvent partial pressure. The chamber has two copper planchettes at the top and bottom thereof. A sample is placed in the chamber on a grid and is squeezed between the planchettes into a thin film. The thin film is placed in a liquid to vitrify the sample. The sample is removed from the planchettes to fracture the sample. The sample is then placed on a cold stage; and imaged.

摘要翻译： 粘性表面活性剂中间相方法的改进冷冻直接成像。 提供具有受控温度和溶剂分压的室。 该室在其顶部和底部具有两个铜制平面。 将样品放置在格栅上的腔室中，并在平面片之间挤压成薄膜。 将薄膜放置在液体中以使样品玻璃化。 从样品中取出样品以破碎样品。 然后将样品置于冷平台上; 并成像。

公开(公告)号：US20060068373A1

公开(公告)日：2006-03-30

申请号：US11232597

申请日：2005-09-22

申请人： Arijit Bose ,  Nazneen Aziz

发明人： Arijit Bose ,  Nazneen Aziz

IPC分类号： C12Q1/00 ,  C12Q1/68 ,  C12M1/34

CPC分类号： G01N1/42 ,  B01L3/022 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/68 ,  G01N2001/2873 ,  H01J2237/2001 ,  H01J2237/204

摘要： A system for generating an image of ultrastructural biomarkers from a biological sample is provided. The system includes a grid onto which a sample to be imaged may be placed and a cryogenic reservoir into which the grid and sample may be immersed for vitrification of the sample. The system also includes a stage onto which the grid and sample may be situated for subsequent imaging in a high contrast imager to permit identification of ultrastructural biomarkers therein. A method for generating an image of ultrastructural biomarkers from a biological sample is also provided. The generated image of ultrastructural biomarkers may be used subsequently for screening and monitoring diseases, evaluating drug and therapeutic efficacy, and assessing risks associated with a drug or therapeutic candidate, among other things.

公开(公告)号：US06635181B2

公开(公告)日：2003-10-21

申请号：US09933381

申请日：2001-08-20

申请人： Arijit Bose

发明人： Arijit Bose

IPC分类号： B01D3506

CPC分类号： B03C1/01 ,  B03C1/035 ,  B03C1/23 ,  B03C1/288

摘要： A continuous, hybrid magnetic field gradient device for colloidal magnetic affinity separation having an axially-rotating horizontal glass tube, and a plurality of axially located repeating magnetic units. Each magnetic units consists of an alternating current solenoid that surrounds the chamber followed by computer-controlled electromagnets carrying a direct current. The on-off cycle of the electromagnets is used to control the residence time of target-bound magnetic particles in the chamber thereby allowing the separate collection of the particle and target rich fractions and the target-lean fractions without interrupting the feed flow. The azimuthally flowing alternating current in the solenoid introduces transient axial and radial forces as well as torque on the magnetic particles, promoting mixing.

公开(公告)号：US20090196484A1

公开(公告)日：2009-08-06

申请号：US12367194

申请日：2009-02-06

申请人： Arijit Bose ,  Nazneen Aziz

发明人： Arijit Bose ,  Nazneen Aziz

IPC分类号： G06K9/00

CPC分类号： G01N1/42 ,  B01L3/022 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/68 ,  G01N2001/2873 ,  H01J2237/2001 ,  H01J2237/204

摘要： A system for generating an image of ultrastructural biomarkers from a biological sample is provided. The system includes a grid onto which a sample to be imaged may be placed and a cryogenic reservoir into which the grid and sample may be immersed for vitrification of the sample. The system also includes a stage onto which the grid and sample may be situated for subsequent imaging in a high contrast imager to permit identification of ultrastructural biomarkers therein. A method for generating an image of ultrastructural biomarkers from a biological sample is also provided. The generated image of ultrastructural biomarkers may be used subsequently for screening and monitoring diseases, evaluating drug and therapeutic efficacy, and assessing risks associated with a drug or therapeutic candidate, among other things.