公开(公告)号：US5989882A

公开(公告)日：1999-11-23

申请号：US816105

申请日：1997-03-14

申请人： Anneliese Crueger ,  Hans-Georg Dellweg ,  Jurgen Georg Lenz ,  Werner Schroder ,  Hermann Pape ,  Klaus Goeke ,  Beate Schaper ,  Michael Hemker ,  Wolfgang Piepersberg ,  Jurgen Distler ,  Ansgar Stratmann

发明人： Anneliese Crueger ,  Hans-Georg Dellweg ,  Jurgen Georg Lenz ,  Werner Schroder ,  Hermann Pape ,  Klaus Goeke ,  Beate Schaper ,  Michael Hemker ,  Wolfgang Piepersberg ,  Jurgen Distler ,  Ansgar Stratmann

IPC分类号： C12N15/09 ,  C07H3/06 ,  C07H15/00 ,  C07H21/04 ,  C12N9/10 ,  C12N15/00 ,  C12N15/11 ,  C12N15/31 ,  C12N15/52 ,  C12N15/54 ,  C12N15/76 ,  C12P19/12 ,  C12P19/18 ,  C12P19/26 ,  C12R1/045 ,  C12R1/19 ,  C12N1/20

CPC分类号： C12P19/26 ,  C12N9/1048

摘要： The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expression of the acarviosyl transferase in a heterologous host organism, to the use of the acarviosyl transferase for converting acarbose minor constituents into acarbose or for preparing acarbose homologues, to the use of the acarviosyl transferase in acarbose purification, and also to the preparation of production mutants in which formation of minor constituents is reduced by means of inactivation of the acarviosyl transferase gene.

摘要翻译： 本发明涉及来自放线菌的主要来自Actinoplanes sp。的acarviosyl转移酶。 SE 50/110及其突变体，用于分离，纯化和表征酶的过程，分离和表征编码α-乙酰氧基转移酶的acbD基因到异源宿主生物体中的阿卡非糖转移酶的表达，使用 的阿卡波糖转移酶用于将阿卡波糖次要成分转化成阿卡波糖或用于制备阿卡波糖同系物，以及在阿卡波糖纯化中使用阿卡波糖转移酶，以及制备生产突变体，其中通过灭活 阿维生素转移酶基因。