公开(公告)号：US08237131B2

公开(公告)日：2012-08-07

申请号：US11665768

申请日：2005-10-21

Applicant: Bertrand Viellerobe ,  Francois Lacombe ,  Alexandre Loiseau ,  Frederic Louradour ,  Mickael Lelek ,  Alain Barthelemy ,  Dominique Pagnoux

Inventor： Bertrand Viellerobe ,  Francois Lacombe ,  Alexandre Loiseau ,  Frederic Louradour ,  Mickael Lelek ,  Alain Barthelemy ,  Dominique Pagnoux

IPC: G21K5/04

CPC classification number: G02B23/2484 ,  A61B5/0062 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0086 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6417 ,  G01N2021/6484 ,  G01N2201/08 ,  G01N2201/1053 ,  G02B6/04 ,  G02B21/002 ,  G02B21/16 ,  G02B23/2469

Abstract: A system for carrying out fibered multiphoton microscopic imagery of a sample (10) for use in endoscopy or fluorescence microscopy includes: a femtosecond pulsed laser (1, 2) for generating a multiphoton excitation laser radiation; an image guide (8) having a number of optical fibers and permitting the sample to be illuminated by a point-by-point scanning in a subsurface plane; pre-compensating elements (4) for pre-compensating for dispersion effects of the excitation pulses in the image guide (8), these elements being situated between the pulsed laser and the image guide (8); scanning elements for directing, in succession, the excitation laser beam in a fiber of the image guide, and; in particular, an optical head (9) for focussing the excitation laser beam exiting the image guide in the sample (10).

Abstract translation: 用于进行用于内窥镜检查或荧光显微镜的样品（10）的纤维多光子显微镜图像的系统包括：用于产生多光子激发激光辐射的飞秒脉冲激光器（1,2） 具有多个光纤的图像引导件（8），并允许通过在地下平面中的逐点扫描照亮样品; 预补偿元件（4），用于对图像引导件（8）中的激励脉冲的色散效应进行预补偿，这些元件位于脉冲激光器和图像引导件（8）之间; 用于连续地引导图像引导件的光纤中的激发激光束的扫描元件，以及 特别地，用于聚焦离开图像引导件的激发激光束在样品（10）中的光学头（9）。

公开(公告)号：US08577445B2

公开(公告)日：2013-11-05

申请号：US10539519

申请日：2003-12-12

Applicant: Frederic Berier ,  Bertrand Viellerobe ,  Magalie Genet ,  Francois Lacombe ,  Pedro Santos

Inventor： Frederic Berier ,  Bertrand Viellerobe ,  Magalie Genet ,  Francois Lacombe ,  Pedro Santos

IPC: A61B5/05

CPC classification number: G02B21/0072 ,  A61B1/0008 ,  A61B1/00172 ,  G02B21/0028 ,  G02B23/2407 ,  G02B26/0875 ,  G02B26/101

Abstract: An optical head includes: a point source producing an excitation beam, optical elements adapted to converge the optical beam into an excitation point located in a subsurface plane relative to the surface of a sample, the plane being perpendicular to the optical axis of the optical head; and elements for scanning the excitation point so as to define an observation field in the subsurface plane along two perpendicular scanning directions, a rapid online scanning and a slow columnar scanning. The invention includes micro-electrical mechanical systems designed to move in translation along a selected displacement at least one of the optical elements, which is mobile along a direction perpendicular to the optical axis so as to obtain at least one of the scanning directions. The invention provides the advantages of maintaining an axial illumination of the sample and of using a miniature head.

Abstract translation: 光头包括：产生激发光束的点光源，适于将光束会聚到位于相对于样品表面的地下平面内的激发点的光学元件，该平面垂直于光学头的光轴 ; 以及用于扫描激发点的元件，以便沿着两个垂直扫描方向在地下平面中定义观察场，快速在线扫描和慢速柱状扫描。 本发明包括微电机械系统，其被设计成沿着沿着与光轴垂直的方向移动的至少一个光学元件的选定位移平移地移动，以便获得扫描方向中的至少一个。 本发明提供了保持样品的轴向照明和使用微型头的优点。

公开(公告)号：US07285089B2

公开(公告)日：2007-10-23

申请号：US10500160

申请日：2002-12-20

Applicant: Bertrand Viellerobe ,  Magalie Genet ,  Frédéric Berier ,  François Lacombe ,  Aymeric Perchant ,  Georges Le Goualher ,  Sandra Marti ,  Stéphane Bourriaux

Inventor： Bertrand Viellerobe ,  Magalie Genet ,  Frédéric Berier ,  François Lacombe ,  Aymeric Perchant ,  Georges Le Goualher ,  Sandra Marti ,  Stéphane Bourriaux

IPC: A61B1/06 ,  G02B26/08

CPC classification number: A61B1/00188 ,  A61B1/00165 ,  A61B1/042 ,  A61B1/07 ,  A61B2562/0242 ,  G02B23/2469

Abstract: Equipment includes an image guide (1) consisting of flexible optical fibers with: on the proximal end side: a source (2), angular scanning elements (3), injection elements (4) in one of the fibers, elements for splitting (5) the illuminating beam and the backscattered signal, elements for spatial filtering (6), elements for detecting (7) the signal, electronic elements (8) for controlling, analyzing and digital processing of the detected signal and display; and on the distal end side: an optical head (9) for focusing the illuminating beam exiting from the illuminated fiber. The scanning elements include a resonant line mirror (M1) and a galvanometric field mirror (M2) with a variable frequency and two afocal optical systems adapted to conjugate the two mirrors (M1, M2) firstly in the field mirror (M2) and the injection elements (4) in the image guide in a second step.

Abstract translation: 设备包括由柔性光纤组成的图像引导件（1），其具有：在基端侧：源（2），角扫描元件（3），其中一个光纤中的注入元件（4），用于分离的元件（5 ）照明光束和反向散射信号，用于空间滤波的元件（6），用于检测信号的元件（7），用于控制，分析和数字处理检测信号和显示的电子元件（8）; 并且在远端侧：用于聚焦从照明光纤出射的照明光束的光学头（9）。 扫描元件包括谐振线路反射镜（M1）和具有可变频率的电流计镜（M 2）和适于首先在场反射镜（M 2）和图像引导件中的注入元件（4）。

公开(公告)号：US07221824B2

公开(公告)日：2007-05-22

申请号：US10499987

申请日：2002-12-20

Applicant: Frederic Berier ,  Stephane Bourriaux ,  Magalie Genet ,  Bertrand Viellerobe ,  Alexandre Loiseau ,  Benjamin Abrat

Inventor： Frederic Berier ,  Stephane Bourriaux ,  Magalie Genet ,  Bertrand Viellerobe ,  Alexandre Loiseau ,  Benjamin Abrat

IPC: G02B6/32 ,  G02B6/06 ,  G02B27/02

CPC classification number: A61B1/00188 ,  A61B2562/0242 ,  A61B2562/146

Abstract: An optical head for equipping the distal end of a flexible optical fiber bundle, designed to be urged into contact with an analyzing surface and including optical elements for focusing an excitation signal into a so-called excitation focal point located at a specific depth beneath the analyzing surface and for sampling a signal backscattered by the excitation focal point which is carried back by the fiber bundle. The head includes an optics-holder tube wherein are inserted on one side the distal end portion of the fiber bundle and on the other optical elements, the latter including a plate placed in contact with the end of the fiber bundle whereof the index is close to that of the fiber core and a focusing optical block, an output window being further provided adapted to provide index adaptation so as to eliminate parasitic reflection occurring on the analyzing surface.

Abstract translation: 一种用于装备柔性光纤束的远端的光学头，设计成被推动与分析表面接触并且包括用于将激发信号聚焦到位于分析器下方特定深度处的所谓的激发焦点的光学元件 表面，并对由纤维束承载的激发焦点反向散射的信号采样。 头部包括光纤保持器管，其中插入在纤维束的远端部分的一侧和另一个光学元件上，其中光学元件包括与纤维束的端部接触的板，其中指数接近 光纤芯和聚焦光学块的输出窗口进一步提供适于提供指标适应以消除在分析表面上发生的寄生反射。

公开(公告)号：US20060256194A1

公开(公告)日：2006-11-16

申请号：US10539572

申请日：2003-12-12

Applicant: Bertrand Viellerobe ,  Frederic Berier ,  Jean-Michel Boniface

Inventor： Bertrand Viellerobe ,  Frederic Berier ,  Jean-Michel Boniface

IPC: H04N7/18

CPC classification number: G02B21/008 ,  G02B21/0028 ,  G02B21/0032 ,  G02B23/2423

Abstract: A parallel confocal laser microscopy system (2) includes a VCSEL vertical cavity laser array (23) for emitting light beams, optical elements (24) for focusing the light beams onto an object (25) to be observed. The invention is characterized in that a photodetector (22) is arranged behind each VCSEL laser such that the photodetector is capable of receiving a light beam backscattered from the object (25) via the VCSEL laser cavity, the cavity having an opening acting as filtering hole.

Abstract translation: 平行共焦激光显微镜系统（2）包括用于发射光束的VCSEL垂直腔激光器阵列（23），用于将光束聚焦到待观察的物体（25）上的光学元件（24）。 本发明的特征在于，在每个VCSEL激光器之后设置光电检测器（22），使得光电检测器能够经由VCSEL激光器腔从物体（25）反射散射的光束，空腔具有用作滤光孔的开口 。

公开(公告)号：US08808171B2

公开(公告)日：2014-08-19

申请号：US12300247

申请日：2007-05-11

Applicant: Francois Lacombe ,  Bertrand Viellerobe ,  Nicolas Boularot ,  Francois Doussoux ,  Aymeric Perchant

Inventor： Francois Lacombe ,  Bertrand Viellerobe ,  Nicolas Boularot ,  Francois Doussoux ,  Aymeric Perchant

IPC: A61B1/06 ,  A61B1/00 ,  A61B1/04 ,  G02B23/26 ,  G02B6/06 ,  A61B5/00 ,  A61B1/313

CPC classification number: G02B23/26 ,  A61B1/00165 ,  A61B1/043 ,  A61B1/313 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  G02B6/06

Abstract: An endoscopy device includes several light guides (1, 2, 3), each light guide having one or more optical fibers. The light guides are coupled with a sweeping system (4) arranged to direct an excitation light beam in alternation in one of the fibers of the guides from the proximal end of the guide including the one of the fibers. In this way, the proximal ends of the guides may make use of the same sweeping system, whereas the distal ends of the guides may be installed simultaneously in different zones of interest of an object or animal (10) under study for an almost simultaneous observation of the zones of interest. The invention also relates to an endoscopy method used in a device according to the invention. A device and a method according to the invention may be applied to quasi-simultaneous reflectance, fluorescence, multi-photon imaging of several areas of interest.

Abstract translation: 内窥镜检查装置包括若干个光导（1,2,3），每个光导具有一根或多根光纤。 光导件与扫掠系统（4）耦合，所述清扫系统（4）被布置成从引导件的近端引导引导光纤中的一个纤维中的激发光束，所述光纤包括所述一个光纤。 以这种方式，引导件的近端可以使用相同的扫掠系统，而引导件的远端可以同时安装在正在研究的几乎同时观察的物体或动物（10）的不同感兴趣的区域中 的感兴趣区域。 本发明还涉及在根据本发明的装置中使用的内窥镜检查方法。 根据本发明的装置和方法可以应用于若干感兴趣区域的准同时反射，荧光，多光子成像。

公开(公告)号：US07869679B2

公开(公告)日：2011-01-11

申请号：US12249665

申请日：2008-10-10

Applicant: Bertrand Viellerobe ,  Francois Lacombe ,  Nicolas Boularot ,  Francois Doussoux ,  Nicolas Lavillonniere

Inventor： Bertrand Viellerobe ,  Francois Lacombe ,  Nicolas Boularot ,  Francois Doussoux ,  Nicolas Lavillonniere

IPC: G02B6/06

CPC classification number: G01N21/6458 ,  A61B1/00165 ,  A61B1/00186 ,  A61B1/043 ,  A61B1/0638 ,  A61B1/07 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/0084 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2201/024 ,  G01N2201/0846 ,  G01N2201/086

Abstract: An imaging device includes an illumination module comprising at least one emitter for emitting at least one excitation beam; a scanning and injection module comprising an image guide, a proximal end and a distal end of which are linked by a plurality of optical fibers; a scanning and injection optical system configured to alternately inject the at least one excitation beam into an optical fiber of the image guide from the proximal end of the image guide; a detection module comprising a detector for detecting a luminous flux collected at the distal end of the image guide, wherein at least one of the illumination module and the detection module is optically conjugated with the scanning and injection module using a conjugating optical fiber.

Abstract translation: 一种成像装置包括：照明模块，包括至少一个用于发射至少一个激发光束的发射器; 扫描和注射模块，包括图像引导件，其近端和远端由多根光纤连接; 扫描和注射光学系统，被配置为从所述图像引导件的近端交替地将所述至少一个激发光束注入到所述图像引导件的光纤中; 检测模块，包括用于检测在图像引导件的远端处收集的光通量的检测器，其中照明模块和检测模块中的至少一个使用共轭光纤与扫描和注射模块光学共轭。

公开(公告)号：US20100234686A1

公开(公告)日：2010-09-16

申请号：US12300247

申请日：2007-05-11

Applicant: Francois Lacombe ,  Bertrand Viellerobe ,  Nicolas Boularot ,  Francois Doussoux ,  Aymeric Perchant

Inventor： Francois Lacombe ,  Bertrand Viellerobe ,  Nicolas Boularot ,  Francois Doussoux ,  Aymeric Perchant

IPC: A61B1/06 ,  G02B6/04

CPC classification number: G02B23/26 ,  A61B1/00165 ,  A61B1/043 ,  A61B1/313 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  G02B6/06

Abstract: An endoscopy device includes several light guides (1, 2, 3), each light guide having one or more optical fibres. The light guides are coupled with a sweeping system (4) arranged to direct an excitation light beam in alternation in one of the fibres of the guides from the proximal end of the guide including the one of the fibres. In this way, the proximal ends of the guides may make use of the same sweeping system, whereas the distal ends of the guides may be installed simultaneously in different zones of interest of an object or animal (10) under study for an almost simultaneous observation of the zones of interest. The invention also relates to an endoscopy method used in a device according to the invention. A device and a method according to the invention may be applied to quasi-simultaneous reflectance, fluorescence, multi-photon imaging of several areas of interest.

Abstract translation: 内窥镜检查装置包括若干个光导（1,2,3），每个光导具有一根或多根光纤。 光导件与扫掠系统（4）耦合，所述清扫系统（4）被布置成从引导件的近端引导引导光纤中的一个纤维中的激发光束，所述光纤包括所述一个光纤。 以这种方式，引导件的近端可以使用相同的扫掠系统，而引导件的远端可以同时安装在正在研究的几乎同时观察的物体或动物（10）的不同感兴趣的区域中 的感兴趣区域。 本发明还涉及在根据本发明的装置中使用的内窥镜检查方法。 根据本发明的装置和方法可以应用于若干感兴趣区域的准同时反射，荧光，多光子成像。

公开(公告)号：US20070290145A1

公开(公告)日：2007-12-20

申请号：US11665768

申请日：2005-10-21

Applicant: Bertrand Viellerobe ,  Francois Lacombe ,  Alexandre Loiseau ,  Frederic Louradour ,  Mikael Lelek ,  Alain Barthelemy ,  Domoinique Pagnoux

Inventor： Bertrand Viellerobe ,  Francois Lacombe ,  Alexandre Loiseau ,  Frederic Louradour ,  Mikael Lelek ,  Alain Barthelemy ,  Domoinique Pagnoux

IPC: G21K5/04 ,  F21K2/00 ,  F21V9/16

CPC classification number: G02B23/2484 ,  A61B5/0062 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0086 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6417 ,  G01N2021/6484 ,  G01N2201/08 ,  G01N2201/1053 ,  G02B6/04 ,  G02B21/002 ,  G02B21/16 ,  G02B23/2469

Abstract: A system for carrying out fibered multiphoton microscopic imagery of a sample (10) for use in endoscopy or fluorescence microscopy includes: a femtosecond pulsed laser (1, 2) for generating a multiphoton excitation laser radiation; an image guide (8) having a number of optical fibers and permitting the sample to be illuminated by a point-by-point scanning in a subsurface plane; pre-compensating elements (4) for pre-compensating for dispersion effects of the excitation pulses in the image guide (8), these elements being situated between the pulsed laser and the image guide (8); scanning elements for directing, in succession, the excitation laser beam in a fiber of the image guide, and; in particular, an optical head (9) for focussing the excitation laser beam exiting the image guide in the sample (10).

Abstract translation: 用于进行用于内窥镜检查或荧光显微镜检查的样品（10）的纤维多光子显微镜图像的系统包括：用于产生多光子激发激光辐射的飞秒脉冲激光器（1,2） 具有多个光纤的图像引导件（8），并允许通过在地下平面中的逐点扫描照亮样品; 预补偿元件（4），用于对图像引导件（8）中的激励脉冲的色散效应进行预补偿，这些元件位于脉冲激光器和图像引导件（8）之间; 用于连续地引导图像引导件的光纤中的激发激光束的扫描元件，以及 特别地，用于聚焦离开图像引导件的激发激光束在样品（10）中的光学头（9）。

公开(公告)号：US08081310B2

公开(公告)日：2011-12-20

申请号：US11629213

申请日：2005-06-09

Applicant: Bertrand Viellerobe ,  Florence Jean ,  Genevieve Bourg-Heckly ,  Alexandre Loiseau

Inventor： Bertrand Viellerobe ,  Florence Jean ,  Genevieve Bourg-Heckly ,  Alexandre Loiseau

IPC: G01N21/64

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N2021/6417 ,  G01N2021/6423

Abstract: The invention relates to a method for producing a fluorescent fiber image of a sample, wherein a sample is scanned with the aid of an excitation signal; the fluorescent signal emanating from the sample is detected, wherein the excitation signal and fluorescent signal use the same optical path; the optical path is used to excite at least two fluorophores contained in the sample; a final image is produced, including areas that are colored according to the at least two fluorophores. The multimarking according to the invention makes it possible to simultaneously acquire two images in two different wavelength bands. The system according to the invention can comprise a spectrometer for spectral quantification of the fluorescent signal.

Abstract translation: 本发明涉及一种用于制备样品的荧光纤维图像的方法，其中借助于激发信号扫描样品; 检测从样品发出的荧光信号，其中激发信号和荧光信号使用相同的光路; 光路用于激发样品中包含的至少两个荧光团; 产生最终图像，包括根据至少两个荧光团着色的区域。 根据本发明的多标记使得可以同时获取两个不同波长带中的两个图像。 根据本发明的系统可以包括用于荧光信号的光谱定量的光谱仪。