公开(公告)号：US06261848B1

公开(公告)日：2001-07-17

申请号：US09074644

申请日：1998-05-08

申请人： Charles W. Anderson ,  C. Brent Bargeron ,  Richard C. Benson ,  Micah A. Carlson ,  Allan B. Fraser ,  John D. Groopman ,  Harvey W. Ko ,  David R. Kohler ,  Terry E. Phillips ,  Paul T. Strickland

发明人： Charles W. Anderson ,  C. Brent Bargeron ,  Richard C. Benson ,  Micah A. Carlson ,  Allan B. Fraser ,  John D. Groopman ,  Harvey W. Ko ,  David R. Kohler ,  Terry E. Phillips ,  Paul T. Strickland

IPC分类号： G01N33543

CPC分类号： G01N1/40 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2400/0481 ,  G01N1/405 ,  G01N21/6428 ,  G01N21/645 ,  G01N30/02 ,  G01N33/54373 ,  G01N33/582 ,  G01N2001/022 ,  G01N2201/0221 ,  B01D15/3804

摘要： A hand held, self-contained, automatic, low power and rapid sensor platform for detecting and quantifying a plurality of analytes. A sample solution potentially containing an unknown amount of an analyte is passed through an affinity column which contains antibodies to which the analyte binds thereby extracting the analyte. The affinity column is then rinsed to remove any other chemicals that may fluoresce. The rinsed affinity column is then eluted with a known volume of elution fluid causing the analyte to release from the antibody and dissolve in the fluid (eluant). The eluant is then placed in the quartz cuvette of a fluorometer. The analyte suspended in the eluant fluoresces at a waveband which is different than that of the light source that excites it. The amount of fluorescence is measured and the level of analyte determined. The rinsing and elution fluids, and/or the affinity column can be placed in a module for easy insertion and removal from the sensor when depleted or when the sensor is to be used against a different analyte.

公开(公告)号：US4148585A

公开(公告)日：1979-04-10

申请号：US767883

申请日：1977-02-11

申请人： C. Brent Bargeron ,  Owen J. Deters

发明人： C. Brent Bargeron ,  Owen J. Deters

IPC分类号： G01P5/26 ,  G01P3/36

CPC分类号： G01P5/26

摘要： A laser Doppler velocimeter which can measure fluid flow in three dimensions, employing a laser beam projected through a rotating diffraction grating and spaced lenses, with a beam splitter in the major optical path between two lenses ahead of the flow channel through which the fluid passes. Selected pairs of laser-derived beams are directed through the fluid and intersect at a predetermined point in the fluid. The movement of the particles through interference patterns formed by the intersecting laser-derived beams generates further-modulated scattered light, which is focused on a photomultiplier tube, from the output of which a fluid velocity directional component signal is derived for each pair. There are four laser-divided beams, and different pairs of these beams can be selected, to provide measurements from which the components of the fluid velocity along three mutually orthogonal directions can be derived.

摘要翻译： 一种激光多普勒测速仪，其可以使用通过旋转衍射光栅投射的激光束和间隔开的透镜来测量三维流体流动，其中分束器在流体通过的流动通道之前的两个透镜之间的主光路中。 所选择的一对激光衍生的光束被引导通过流体并在流体中的预定点处相交。 通过由相交的激光衍生的光束形成的干涉图案，颗粒的运动从其输出中产生进一步调制的散射光，该散射光聚焦在光电倍增管上，其输出对于每对导出流体速度方向分量信号。 有四个激光分束，并且可以选择不同对的这些光束，以提供可以从中导出沿着三个相互正交的方向的流体速度的分量的测量。

公开(公告)号：US06969605B2

公开(公告)日：2005-11-29

申请号：US09906243

申请日：2001-07-16

申请人： Charles W. Anderson ,  C. Brent Bargeron ,  Richard C. Benson ,  Micah A. Carlson ,  Allan B. Fraser ,  John D. Groopman ,  Harvey W. Ko ,  David R. Kohler ,  Terry E. Phillips ,  Paul T. Strickland

发明人： Charles W. Anderson ,  C. Brent Bargeron ,  Richard C. Benson ,  Micah A. Carlson ,  Allan B. Fraser ,  John D. Groopman ,  Harvey W. Ko ,  David R. Kohler ,  Terry E. Phillips ,  Paul T. Strickland

IPC分类号： B01L3/00 ,  G01N1/02 ,  G01N1/40 ,  G01N21/64 ,  G01N21/76 ,  G01N21/78 ,  G01N30/02 ,  G01N33/543 ,  G01N33/58 ,  C12M3/00

CPC分类号： G01N1/40 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2400/0481 ,  G01N1/405 ,  G01N21/6428 ,  G01N21/645 ,  G01N30/02 ,  G01N33/54373 ,  G01N33/582 ,  G01N2001/022 ,  G01N2201/0221 ,  B01D15/3804

摘要： A hand held, self-contained, automatic, low power and rapid sensor platform for detecting and quantifying a plurality of analytes. A sample solution potentially containing an unknown amount of an analyte is passed through an affinity column which contains antibodies to which the analyte binds thereby extracting the analyte. The affinity column is then rinsed to remove any other chemicals that may fluoresce. The rinsed affinity column is then eluted with a known volume of elution fluid causing the analyte to release from the antibody and dissolve in the fluid (eluant). The eluant is then placed in the quartz cuvette of a fluorometer. The analyte suspended in the eluant fluoresces at a waveband which is different than that of the light source that excites it. The amount of fluorescence is measured and the level of analyte determined. The rinsing and elution fluids, and/or the affinity column can be placed in a module for easy insertion and removal from the sensor when depleted or when the sensor is to be used against a different analyte.

摘要翻译： 用于检测和量化多种分析物的手持式，独立的，自动的，低功率和快速的传感器平台。 可能含有未知量的分析物的样品溶液通过亲和柱，其包含分析物结合的抗体，从而提取分析物。 然后漂洗亲和柱以除去可能发荧光的任何其他化学物质。 然后用已知体积的洗脱液洗脱漂洗的亲和柱，使得分析物从抗体中释放并溶解在流体（洗脱剂）中。 然后将洗脱液置于荧光计的石英比色杯中。 悬浮在洗脱液中的分析物以与激发它的光源不同的波段发出荧光。 测量荧光的量并测定分析物的水平。 冲洗和洗脱流体和/或亲和柱可以放置在模块中，以便在耗尽时或当传感器用于抵抗不同分析物时容易地从传感器中插入和移除。