公开(公告)号：US08226826B2

公开(公告)日：2012-07-24

申请号：US12457918

申请日：2009-06-25

Applicant: Andrew M. Wo ,  Chen-Lin Chen ,  Ken-Chao Chen ,  Yu-Cheng Pan

Inventor： Andrew M. Wo ,  Chen-Lin Chen ,  Ken-Chao Chen ,  Yu-Cheng Pan

IPC: B01D35/06 ,  C12M1/34

CPC classification number: C12N1/02 ,  C12M47/04 ,  G01N35/00069 ,  G01N2035/00495 ,  Y10T436/111666 ,  Y10T436/25375

Abstract: Disclosed is a disk based system for separating at least two types of particulates contained in a sample fluid. The system includes a disk-like carrier board and a magnetic attraction unit. The disk-like carrier board forms at least one flow channel structure, which includes an inner reservoir, at least one separation chamber, and at least one outer reservoir arranged in sequence from a geometric center of the disk-like carrier board to an outer circumferential rim of the disk-like carrier board. A method of separation carried out with the system includes introducing the sample fluid into the inner reservoir and then rotating the disk-like carrier board to induce a centrifugal force. The sample fluid contains particulates that are labeled with immunomagnetic beads and the labeled particulates are attracted by the magnetic force generated by the magnetic attraction unit to retain in the inner reservoir or the separation chamber. Particulates not labeled with the immunomagnetic beads and contained in the sample fluid move with the sample fluid to the outer reservoir.

Abstract translation: 公开了一种用于分离包含在样品流体中的至少两种类型的微粒的基于盘的系统。 该系统包括盘状载体板和磁性吸引单元。 盘形载体板形成至少一个流动通道结构，其包括内部储存器，至少一个分离室和至少一个外部储存器，其依次从盘状载体板的几何中心排列到外部圆周 盘状载板的边缘。 用系统进行的分离方法包括将样品流体引入内部储存器中，然后旋转盘状载体板以引起离心力。 样品流体包含用免疫磁珠标记的微粒，并且标记的微粒被磁吸引单元产生的磁力吸引，以保持在内储存器或分离室中。 未标记有免疫磁珠并且包含在样品流体中的颗粒与样品流体一起移动到外部储存器。

公开(公告)号：US20120039769A1

公开(公告)日：2012-02-16

申请号：US13104307

申请日：2011-05-10

Applicant: ANDREW MAN CHUNG WO ,  CHEN-LIN CHEN ,  CHENG WEI YANG ,  YU-CHENG PAN

Inventor： ANDREW MAN CHUNG WO ,  CHEN-LIN CHEN ,  CHENG WEI YANG ,  YU-CHENG PAN

IPC: G01N1/10

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2300/045 ,  B01L2300/048 ,  B01L2300/0803 ,  B01L2400/0409 ,  B01L2400/0644 ,  G01N35/00069 ,  G01N2035/0449 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A fluid sample collection device for a disk-based fluid separation system is disclosed. The disk-based separation system includes a compact microfluidic disk with at least one flow channel pattern formed on a side surface of the disk. At least one orifice is formed on an outflow boundary of the disk and is designed in fluid communication with the flow channel pattern through a communication channel. The fluid sample collection device includes at least one collection tube having an open end serving as a fluid receiving end and corresponding to the orifice of the disk with a distance. When the disk is rotated, at least a portion of fluid sample in a sample processing reservoir formed on the disk is delivered by centripetal force through the communication channel and the orifice, and finally the expelling fluid sample is collected in the collection tube.

Abstract translation: 公开了一种用于基于盘的流体分离系统的流体样品收集装置。 基于盘的分离系统包括紧凑的微流体盘，其具有形成在盘的侧表面上的至少一个流动通道图案。 在盘的流出边界上形成至少一个孔，并且通过通信通道设计成与流动通道图案流体连通。 流体样品收集装置包括至少一个收集管，该收集管的开口端用作流体接收端并对应于具有一定距离的盘的孔口。 当盘旋转时，形成在盘上的样品处理容器中的流体样品的至少一部分通过向心力传递通过连通通道和孔口，最后将排出流体样品收集在收集管中。

公开(公告)号：US08039249B2

公开(公告)日：2011-10-18

申请号：US12382844

申请日：2009-03-25

Applicant: Andrew M. Wo ,  Chen-Lin Chen ,  Ken-Chao Chen ,  Yu-Cheng Pan

Inventor： Andrew M. Wo ,  Chen-Lin Chen ,  Ken-Chao Chen ,  Yu-Cheng Pan

IPC: C12M1/34

CPC classification number: B01L3/502761 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0803 ,  B01L2400/0409 ,  B01L2400/043

Abstract: Disclosed is a disk based platform for separating and detecting cells that are labeled with immunomagnetic beads. A disk-like carrier board forms therein at least one flow channel structure, which includes an inner reservoir for receiving a sample fluid, an outer reservoir, and a plurality of micro flow channels arranged between and in fluid communication with the inner and outer reservoirs. When the disk-like carrier board spins, cells labeled with immunomagnetic beads are attracted by a magnetic attraction unit that is arranged above the disk-like carrier board and adjacent to the inner reservoir to thereby remain in the inner reservoir, and cells that are not so labeled are acted upon by a centrifugal force induced by the spinning of the disk-like carrier board to flow with the sample fluid from the inner reservoir through the micro flow channels into the outer reservoir.

Abstract translation: 公开了一种用于分离和检测用免疫磁珠标记的细胞的基于盘的平台。 盘形载体板在其中形成至少一个流动通道结构，其包括用于接收样品流体的内部储存器，外部储存器和布置在内部和外部储存器之间并与其流体连通的多个微流体通道。 当盘状载体板旋转时，用免疫磁性珠子标记的细胞被磁性吸引单元吸引，磁吸引单元布置在盘状载体板的上方并与内部储存器相邻，从而保留在内部储存器中，而不是细胞 如此标记的作用是通过由盘状载体板的纺丝引起的离心力而使样品流体从内部储存器通过微流动通道流入外部储存器。

公开(公告)号：US20070275426A1

公开(公告)日：2007-11-29

申请号：US11882965

申请日：2007-08-08

Applicant: Andrew Wo ,  Chen-Lin Chen ,  Yi-Fang Chang

Inventor： Andrew Wo ,  Chen-Lin Chen ,  Yi-Fang Chang

IPC: G01N33/00

CPC classification number: B01L3/50273 ,  B01L2200/0642 ,  B01L2300/0806 ,  B01L2400/0409

Abstract: A disk-like microfluidic structure includes a disk, and a plurality of microfluidic systems formed on the disk and covered by a top sealing layer. Each microfluidic system includes a first and a second supply chamber located near a geometrical center of the disk, and at least one receiving chamber communicably connected to the first and the second supply chambers via a first and a second microchannel, respectively. When the first and the second microchannels have different geometrical sizes and the disk is driven to spin, two fluids separately held in the first and the second supply chambers are centrifugally moved into the receiving chamber via the differently sized first and second microchannels at different speeds and in different quantities to generate in the receiving chamber a fluid mixture having a specific concentration. By changing the geometrical sizes of the first and second microchannels, different concentration fluid mixtures may be generated.

Abstract translation: 盘状微流体结构包括盘，以及形成在盘上并由顶部密封层覆盖的多个微流体系统。 每个微流体系统包括位于盘的几何中心附近的第一和第二供应室，以及分别经由第一和第二微通道可通信地连接到第一和第二供应室的至少一个接收室。 当第一和第二微通道具有不同的几何尺寸并且盘被驱动旋转时，分别保持在第一和第二供应室中的两个流体通过不同尺寸的不同尺寸的第一和第二微通道以不同的速度离心地移动到接收室中， 以不同的量在接收室中产生具有特定浓度的流体混合物。 通过改变第一和第二微通道的几何尺寸，可能会产生不同浓度的流体混合物。

公开(公告)号：US20100151560A1

公开(公告)日：2010-06-17

申请号：US12382844

申请日：2009-03-25

Applicant: Andrew M. Wo ,  Chen-Lin Chen ,  Ken-Chao Chen ,  Yu-Cheng Pan

Inventor： Andrew M. Wo ,  Chen-Lin Chen ,  Ken-Chao Chen ,  Yu-Cheng Pan

IPC: C12M1/34

CPC classification number: B01L3/502761 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0803 ,  B01L2400/0409 ,  B01L2400/043

Abstract: Disclosed is a disk based platform for separating and detecting cells that are labeled with immunomagnetic beads. A disk-like carrier board forms therein at least one flow channel structure, which includes an inner reservoir for receiving a sample fluid, an outer reservoir, and a plurality of micro flow channels arranged between and in fluid communication with the inner and outer reservoirs. When the disk-like carrier board spins, cells labeled with immunomagnetic beads are attracted by a magnetic attraction unit that is arranged above the disk-like carrier board and adjacent to the inner reservoir to thereby remain in the inner reservoir, and cells that are not so labeled are acted upon by a centrifugal force induced by the spinning of the disk-like carrier board to flow with the sample fluid from the inner reservoir through the micro flow channels into the outer reservoir.

Abstract translation: 公开了一种用于分离和检测用免疫磁珠标记的细胞的基于盘的平台。 盘形载体板在其中形成至少一个流动通道结构，其包括用于接收样品流体的内部储存器，外部储存器和布置在内部和外部储存器之间并与其流体连通的多个微流体通道。 当盘状载体板旋转时，用免疫磁性珠子标记的细胞被磁性吸引单元吸引，磁吸引单元布置在盘状载体板的上方并与内部储存器相邻，从而保留在内部储存器中，而不是细胞 如此标记的作用是通过由盘状载体板的纺丝引起的离心力而使样品流体从内部储存器通过微流动通道流入外部储存器。

公开(公告)号：US08535541B2

公开(公告)日：2013-09-17

申请号：US12861989

申请日：2010-08-24

Applicant: Andrew Man Chung Wo ,  Yu-Cheng Pan ,  Ken-Chao Chen ,  Chen-Lin Chen

Inventor： Andrew Man Chung Wo ,  Yu-Cheng Pan ,  Ken-Chao Chen ,  Chen-Lin Chen

IPC: B01D21/26 ,  B01D35/06 ,  G01N33/543

CPC classification number: C12N13/00 ,  G01N33/54326

Abstract: Disclosed is a method for separating immunomagnetic bead labeled particulates. A carrier board is formed with at least one flow channel structure, which includes an inner reservoir, an outer reservoir, and at least one micro flow channel in communication with the inner reservoir and the outer reservoir. The method includes labeling target particulates with immunomagnetic bead, introducing a sample fluid into the inner reservoir, and applying a magnetic force and a driving force, wherein the driving force drives the particulates not labeled with immunomagnetic bead to flow through the micro flow channel to the outer reservoir, while the magnetic force attracts the particulates labeled with the immunomagnetic bead to retain in the inner reservoir. The driving force may be centrifugal force, pressure, or surface tension.

Abstract translation: 公开了分离免疫磁珠标记的微粒的方法。 载体板形成有至少一个流动通道结构，其包括内部储存器，外部储存器和与内部储存器和外部储存器连通的至少一个微流动通道。 该方法包括用免疫磁珠标记目标颗粒，将样品流体引入内部储存器中，以及施加磁力和驱动力，其中驱动力驱动未标记有免疫磁珠的微粒流过微流通道至 外部储存器，而磁力吸引用免疫磁珠标记的微粒保留在内部储存器中。 驱动力可以是离心力，压力或表面张力。

公开(公告)号：US20110059500A1

公开(公告)日：2011-03-10

申请号：US12861989

申请日：2010-08-24

Applicant: ANDREW MAN CHUNG WO ,  YU-CHENG PAN ,  KEN-CHAO CHEN ,  CHEN-LIN CHEN

Inventor： ANDREW MAN CHUNG WO ,  YU-CHENG PAN ,  KEN-CHAO CHEN ,  CHEN-LIN CHEN

IPC: C12N13/00

CPC classification number: C12N13/00 ,  G01N33/54326

Abstract: Disclosed is a method for separating immunomagnetic bead labeled particulates. A carrier board is formed with at least one flow channel structure, which includes an inner reservoir, an outer reservoir, and at least one micro flow channel in communication with the inner reservoir and the outer reservoir. The method includes labeling target particulates with immunomagnetic bead, introducing a sample fluid into the inner reservoir, and applying a magnetic force and a driving force, wherein the driving force drives the particulates not labeled with immunomagnetic bead to flow through the micro flow channel to the outer reservoir, while the magnetic force attracts the particulates labeled with the immunomagnetic bead to retain in the inner reservoir. The driving force may be centrifugal force, pressure, or surface tension.

Abstract translation: 公开了分离免疫磁珠标记的微粒的方法。 载体板形成有至少一个流动通道结构，其包括内部储存器，外部储存器和与内部储存器和外部储存器连通的至少一个微流动通道。 该方法包括用免疫磁珠标记目标颗粒，将样品流体引入内部储存器中，以及施加磁力和驱动力，其中驱动力驱动未标记有免疫磁珠的微粒流过微流通道至 外部储存器，而磁力吸引用免疫磁珠标记的微粒保留在内部储存器中。 驱动力可以是离心力，压力或表面张力。

公开(公告)号：US20120024083A1

公开(公告)日：2012-02-02

申请号：US13104289

申请日：2011-05-10

Applicant: ANDREW MAN CHUNG WO ,  CHEN-LIN CHEN ,  CHENG WEI YANG

Inventor： ANDREW MAN CHUNG WO ,  CHEN-LIN CHEN ,  CHENG WEI YANG

IPC: G01N1/10

CPC classification number: G01N35/00069 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/043 ,  B01L2400/0694 ,  G01N21/07

Abstract: A disk-based fluid sample separation device including at least one air vent forming a part of a flow channel pattern on a microfluidic disk is disclosed. The fluid sample separation device is provided with an air vent sealing cover having at least through hole and is placed on the top surface of the disk. The air vent sealing cover is rotated with respect to the disk either at a first position or a second position. At the first position, the hole of the air vent sealing cover is in correspondence to the air vent of the flow channel pattern to control the sample liquid delivery. At the second position, the air vent of the flow channel pattern is closed. The flow channel pattern includes at least one sample storage reservoir, at least one sample processing reservoir, and at least one communication channel which is in fluid communication between the sample storage reservoir and the sample processing reservoir. In alternative, the status of the hole of the air vent sealing cover is controlled by a control unit.

Abstract translation: 公开了一种基于盘的流体样品分离装置，其包括在微流体盘上形成流路图案的一部分的至少一个排气口。 流体样品分离装置设置有至少具有通孔的排气密封盖，并且放置在盘的顶表面上。 排气密封盖在第一位置或第二位置处相对于盘旋转。 在第一位置，排气孔密封盖的孔对应于流动通道图案的排气口，以控制样品液体输送。 在第二位置，流路图案的排气口关闭。 流动通道图案包括至少一个样品储存储存器，至少一个样品处理储存器以及在样品储存储存器和样品处理储存器之间流体连通的至少一个通信通道。 另外，排气密封盖的孔的状态由控制单元控制。

公开(公告)号：US08524169B2

公开(公告)日：2013-09-03

申请号：US13104307

申请日：2011-05-10

Applicant: Andrew Man Chung Wo ,  Chen-Lin Chen ,  Cheng-Wei Yang ,  Yu-Cheng Pan

Inventor： Andrew Man Chung Wo ,  Chen-Lin Chen ,  Cheng-Wei Yang ,  Yu-Cheng Pan

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2300/045 ,  B01L2300/048 ,  B01L2300/0803 ,  B01L2400/0409 ,  B01L2400/0644 ,  G01N35/00069 ,  G01N2035/0449 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A fluid sample collection device for a disk-based fluid separation system is disclosed. The disk-based separation system includes a compact microfluidic disk with at least one flow channel pattern formed on a side surface of the disk. At least one orifice is formed on an outflow boundary of the disk and is designed in fluid communication with the flow channel pattern through a communication channel. The fluid sample collection device includes at least one collection tube having an open end serving as a fluid receiving end and corresponding to the orifice of the disk with a distance. When the disk is rotated, at least a portion of fluid sample in a sample processing reservoir formed on the disk is delivered by centripetal force through the communication channel and the orifice, and finally the expelling fluid sample is collected in the collection tube.

Abstract translation: 公开了一种用于基于盘的流体分离系统的流体样品收集装置。 基于盘的分离系统包括紧凑的微流体盘，其具有形成在盘的侧表面上的至少一个流动通道图案。 在盘的流出边界上形成至少一个孔，并且通过通信通道设计成与流动通道图案流体连通。 流体样品收集装置包括至少一个收集管，该收集管的开口端用作流体接收端并对应于具有一定距离的盘的孔口。 当盘旋转时，形成在盘上的样品处理容器中的流体样品的至少一部分通过向心力传递通过连通通道和孔口，最后将排出流体样品收集在收集管中。

公开(公告)号：US20100227379A1

公开(公告)日：2010-09-09

申请号：US12457918

申请日：2009-06-25

Applicant: Andrew M. Wo ,  Chen-Lin Chen ,  Ken-Chao Chen ,  Yu-Cheng Pan

Inventor： Andrew M. Wo ,  Chen-Lin Chen ,  Ken-Chao Chen ,  Yu-Cheng Pan

IPC: C12N1/02 ,  C12M1/42

CPC classification number: C12N1/02 ,  C12M47/04 ,  G01N35/00069 ,  G01N2035/00495 ,  Y10T436/111666 ,  Y10T436/25375

Abstract: Disclosed is a disk based system for separating at least two types of particulates contained in a sample fluid. The system includes a disk-like carrier board and a magnetic attraction unit. The disk-like carrier board forms at least one flow channel structure, which includes an inner reservoir, at least one separation chamber, and at least one outer reservoir arranged in sequence from a geometric center of the disk-like carrier board to an outer circumferential rim of the disk-like carrier board. A method of separation carried out with the system includes introducing the sample fluid into the inner reservoir and then rotating the disk-like carrier board to induce a centrifugal force. The sample fluid contains particulates that are labeled with immunomagnetic beads and the labeled particulates are attracted by the magnetic force generated by the magnetic attraction unit to retain in the inner reservoir or the separation chamber. Particulates not labeled with the immunomagnetic beads and contained in the sample fluid move with the sample fluid to the outer reservoir.

Abstract translation: 公开了一种用于分离包含在样品流体中的至少两种类型的微粒的基于盘的系统。 该系统包括盘状载体板和磁性吸引单元。 盘形载体板形成至少一个流动通道结构，其包括内部储存器，至少一个分离室和至少一个外部储存器，其依次从盘状载体板的几何中心排列到外部圆周 盘状载板的边缘。 用系统进行的分离方法包括将样品流体引入内部储存器中，然后旋转盘状载体板以引起离心力。 样品流体包含用免疫磁珠标记的微粒，并且标记的微粒被磁吸引单元产生的磁力吸引，以保持在内储存器或分离室中。 未标记有免疫磁珠并且包含在样品流体中的颗粒与样品流体一起移动到外部储存器。