公开(公告)号：US06346101B1

公开(公告)日：2002-02-12

申请号：US08887196

申请日：1997-06-04

Applicant: Robert R. Alfano ,  Cheng Hui Liu

Inventor： Robert R. Alfano ,  Cheng Hui Liu

IPC: A61B1818

CPC classification number: C12M35/02 ,  C12N15/87

Abstract: A photon-mediated technique for introducing biological materials into cells and/or cellular components. The technique may be used to introduce nucleic acids, such as DNA and RNA, proteins or other biological materials into mammalian cells (as well as into other animal and plant cells), which materials may then flow into the nuclei of the cells. The technique uses picosecond or femtosecond light pulses propagating in the UV, visible and near infrared wavelength regions with powers on the order of 1×1010 W/cm2. In practice, the desired biological materials are coated on the end of the inner core of a single mode fiber or the ring core of a fiber in a fiber array. Each fiber is sized to correspond to one cell, with the core size ranging from 2&mgr; to 10&mgr;, and the cladding ranging from 10&mgr; to 30&mgr;. The laser pulse travels through a fiber core which is coated with the materials and ablates a portion of a targeted cell or cellular component membrane. In addition, as the laser pulse exits the fiber, it imparts energy and momentum to the materials applied to the end of the fiber. Consequently, after ablation of a portion of the membrane, some of the biological materials are caused to enter therethrough.

Abstract translation: 用于将生物材料引入细胞和/或细胞组分的光子介导技术。 该技术可用于将诸如DNA和RNA，蛋白质或其他生物材料的核酸引入哺乳动物细胞（以及其它动物和植物细胞）中，然后该物质可以流入细胞核。 该技术使用在约1×10 10 W / cm 2的功率下在UV，可见光和近红外波长区域中传播的皮秒或飞秒光脉冲。 实际上，将期望的生物材料涂覆在单模纤维的内芯的端部或纤维阵列中的纤维的环形芯上。 每个纤维的尺寸对应于一个电池，其核心尺寸范围为2m至10mu，包层的尺寸为10m至30mu。 激光脉冲穿过纤维芯，涂覆有材料并消融一部分靶细胞或细胞组分膜。 另外，随着激光脉冲离开纤维，它向施加到纤维端部的材料赋予能量和动量。 因此，在一部分膜消融后，一些生物材料被引入其中。

公开(公告)号：US20170049328A1

公开(公告)日：2017-02-23

申请号：US15243607

申请日：2016-08-22

Applicant: Robert R. Alfano ,  Cheng-hui Liu ,  Vidyasagar Sriramoju

Inventor： Robert R. Alfano ,  Cheng-hui Liu ,  Vidyasagar Sriramoju

IPC: A61B5/00 ,  A61B5/02

CPC classification number: A61B5/0086 ,  A61B5/0075 ,  A61B5/02007 ,  A61B2562/0238

Abstract: Vulnerable plaque (VP) is the main cause of death from heart attacks. All currently available methods developed to diagnose VP lack sensitivity and or specificity and are still unable to identify VP. Our patent addresses the problem to diagnose VP in arteries. The teachings here disclose a vulnerable plaque optical analyzer (VPOA) and Imager (VOPAI) for monitoring arterial walls by measuring whether the fingerprint Raman spectrum of adipose (lipid) tissue using Resonance Raman (RR) and common Raman(R) signals of aortic intimal wall layer. The RR and R lines of lipid determine presentation of VP.

Abstract translation: 易受伤害的斑块（VP）是心脏病发作死亡的主要原因。 目前开发用于诊断VP的所有方法缺乏灵敏度和/或特异性，仍然无法识别VP。 我们的专利解决了诊断动脉中VP的问题。 这里的教导公开了通过使用共振拉曼（RR）和主动脉内膜的共同拉曼（R）信号测量脂肪（脂质）组织的指纹拉曼光谱来监测动脉壁的易损斑块光学分析仪（VPOA）和成像仪（VOPAI） 墙层。 脂质的RR和R线决定VP的呈现。

公开(公告)号：US20100317974A1

公开(公告)日：2010-12-16

申请号：US12684739

申请日：2010-01-08

Applicant: Robert R. Alfano ,  Cheng-Hui Liu ,  Wubao Wang ,  Vidyasagar Sriramoju

Inventor： Robert R. Alfano ,  Cheng-Hui Liu ,  Wubao Wang ,  Vidyasagar Sriramoju

IPC: A61B6/00

CPC classification number: A61B5/0075 ,  A61B5/0084 ,  A61B5/02007

Abstract: An apparatus and method of use for detecting vulnerable plaque (VP) in arterial walls is provided. The method includes measuring whether the Raman spectrum of adipose (lipid) tissue signal is present in a Raman signal from aortic intimal wall tissue. The Raman vibration modes for VP are strong bands at about 1435 cm−1, about 2850 cm−1, and about 2892 cm−1 and will be present when the aortic intimal wall tissue is thin.

Abstract translation: 提供了用于检测动脉壁中的易损斑块（VP）的装置和方法。 该方法包括测量脂肪（脂质）组织信号的拉曼光谱是否存在于来自主动脉内膜壁组织的拉曼信号中。 VP的拉曼振动模式在约1435cm-1，约2850cm-1和约2892cm -1处是强带，并且当主动脉内膜壁组织较薄时将呈现。

公开(公告)号：US20190216189A1

公开(公告)日：2019-07-18

申请号：US16015400

申请日：2018-06-22

Applicant: Chun-Chung LIU ,  Cheng-Hui LIU

Inventor： Chun-Chung LIU ,  Cheng-Hui LIU

IPC: A45C3/00 ,  A45C3/06 ,  A45C13/00 ,  A45C13/10 ,  B32B5/02 ,  B32B5/18 ,  B32B5/24 ,  B32B27/06 ,  B32B27/08 ,  B32B27/12 ,  B32B27/30 ,  B32B27/36

CPC classification number: A45C3/001 ,  A45C3/06 ,  A45C13/008 ,  A45C13/103 ,  B32B5/024 ,  B32B5/18 ,  B32B5/245 ,  B32B27/065 ,  B32B27/08 ,  B32B27/12 ,  B32B27/306 ,  B32B27/36 ,  B32B2250/03 ,  B32B2250/24 ,  B32B2250/40 ,  B32B2262/0284 ,  B32B2266/0221 ,  B32B2307/3065 ,  B32B2307/558 ,  B32B2307/581 ,  B32B2307/7265 ,  B32B2439/46

Abstract: A handbag with multiple protective functions includes: a handbag body having front and rear panels and a connection panel connecting the front and rear panels to define a receiving space, wherein a partition layer is disposed in the receiving space to form a plurality of compartments. The connection panel has a plurality of zipper openings disposed at two sides of the partition layer for access into the compartments respectively. Front and rear handles are connected to the front and rear panels, and a shoulder strap having two ends connected to the handbag body. The front and rear panels, the connection panel, the partition layer, the front handle, the rear handle, and the shoulder strap are made of polyester filament yarns fabricated from polyethylene terephthalate so that the handbag has anti-cut, cut-proof, stab-resistant, waterproof, fireproof, and explosion-proof functions.