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    ANALYSIS USING MICROFLUIDIC PARTITIONING DEVICES
    1.
    发明申请
    ANALYSIS USING MICROFLUIDIC PARTITIONING DEVICES 审中-公开
    使用微流化分离装置的分析

    公开(公告)号：US20090317798A1

    公开(公告)日：2009-12-24

    申请号：US11916025

    申请日：2006-06-02

    申请人： Christian A. Heid ,  Antoine Daridon

    发明人： Christian A. Heid ,  Antoine Daridon

    IPC分类号： C12Q1/68

    CPC分类号： B01L3/5027 ,  B01L7/52 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/301

    摘要： The invention relates to methods, reagents and devices for detection and characterization of nucleic acids, cells, and other biological samples. Assay method are provided in which a sample is partitioned into sub-samples, and analysis of the contents of the sub-samples carried out. The invention also provides microfluidic devices for conducting the assay. The invention also provides an analysis method using a universal primers and probes for amplification and detection.

    摘要翻译： 本发明涉及核酸，细胞和其他生物样品的检测和表征的方法，试剂和装置。 提供了将样品分割成子样品并进行子样品的内容的分析的测定方法。 本发明还提供用于进行测定的微流体装置。 本发明还提供了使用通用引物和探针进行扩增和检测的分析方法。

    External control reagents for nucleic acid amplification
    2.
    发明授权
    External control reagents for nucleic acid amplification 有权
    用于核酸扩增的外部对照试剂

    公开(公告)号：US06890718B2

    公开(公告)日：2005-05-10

    申请号：US10099738

    申请日：2002-03-15

    申请人： Christian A. Heid ,  Kenneth J. Livak

    发明人： Christian A. Heid ,  Kenneth J. Livak

    IPC分类号： G01N33/53 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/686 ,  Y10S435/81 ,  C12Q2545/113 ,  C12Q2545/10

    摘要： External control reagents for nucleic acid amplification are provided that verify the absence or presence of specific target sequences, and correct primers and probes. A single-stranded, external control polynucleotide is amplified with primers of the same sequence as target primers. Probes with detectable labels and sequences specific for target and external control polynucleotides allow for detection and measurement. The primers and the detectable probe are adjacent or substantially adjacent when hybridized to the external control polynucleotide. Target and control amplicons may be detected by increased fluorescence induced by polymerase-mediated 5′ nuclease cleavage or hybridization of a self-quenching probe complementary to both target and external control polynucleotides. A kit of PCR reagents can be dispensed into vessels for rapid and accurate nucleic acid amplification assay, with real-time or end-point measurements. The amplification control reagents, kits, and methods of the present invention provide positive and negative control tests which can be conducted concurrently with target amplification. Allelic differences at genetic loci can be detected, including single nucleotide polymorphisms (SNP).

    摘要翻译： 提供用于核酸扩增的外部对照试剂，其验证特定靶序列的不存在或存在，以及校正引物和探针。 使用与靶引物相同序列的引物扩增单链外部对照多核苷酸。 具有可检测标记和具有针对和外部对照多核苷酸序列的序列的探针允许检测和测量。 当与外部对照多核苷酸杂交时，引物和可检测的探针相邻或基本相邻。 可以通过聚合酶介导的5'核酸酶切割诱导的增加的荧光或与靶和外部对照多核苷酸互补的自猝灭探针的杂交来检测靶和对照扩增子。 PCR试剂盒可以分配到容器中，用于快速准确的核酸扩增测定，具有实时或终点测量。 本发明的扩增控制试剂，试剂盒和方法提供可以与靶扩增同时进行的阳性和阴性对照试验。 可以检测遗传基因座中的等位基因差异，包括单核苷酸多态性（SNP）。

    Devices and methods for separating sample constituents
    4.
    发明授权
    Devices and methods for separating sample constituents 有权
    用于分离样品成分的装置和方法

    公开(公告)号：US07704360B2

    公开(公告)日：2010-04-27

    申请号：US11080282

    申请日：2005-03-14

    申请人： Daniel Sobek ,  Christian A. Heid

    发明人： Daniel Sobek ,  Christian A. Heid

    IPC分类号： B01D57/02

    CPC分类号： G01N27/44739 ,  C07K1/26

    摘要： Devices and methods for separating sample constituents are provided. The subject devices are characterized by having a fluid flow path with at least one electrophoretic separation element positioned at a region thereof. The separation element includes an element for applying an electric field across the fluid flow path and a trapping element for trapping sample constituents that migrate out of the flow path when an electric field is applied across the fluid flow path. In using the subject devices, sample is moved past the separation element and an electric field is applied across the flow path such that constituents of the sample migrate into the trapping element. The subject devices and methods find use in a variety of applications, including protein separation applications.

    摘要翻译： 提供了用于分离样品成分的装置和方法。 本发明装置的特征在于具有流体流动路径，其中至少一个电泳分离元件位于其区域。 分离元件包括用于在流体流动路径上施加电场的元件和用于捕获当横跨流体流动路径施加电场时迁移出流动路径的样品成分的捕获元件。 在使用主体装置时，将样品移动通过分离元件，并且跨过流动路径施加电场，使得样品的成分迁移到捕获元件中。 主题设备和方法可用于各种应用，包括蛋白质分离应用。

    Methods for external controls for nucleic acid amplification
    7.
    发明授权
    Methods for external controls for nucleic acid amplification 有权
    用于核酸扩增的外部对照的方法

    公开(公告)号：US06358679B1

    公开(公告)日：2002-03-19

    申请号：US09645959

    申请日：2000-08-24

    申请人： Christian A. Heid ,  Kenneth J. Livak

    发明人： Christian A. Heid ,  Kenneth J. Livak

    IPC分类号： C12Q170

    CPC分类号： C12Q1/686 ,  Y10S435/81 ,  C12Q2545/113 ,  C12Q2545/10

    摘要： Methods of nucleic acid amplification with external controls are provided that verify the absence or presence of specific target sequences, and correct primers and probes. A single-stranded, external control polynucleotide is amplified with primers of the same sequence as target primers. Probes with detectable labels and sequences specific for target and external control polynucleotides allow for detection and measurement. The primers and the detectable probe are adjacent or substantially adjacent when hybridized to the external control polynucleotide. Target and control amplicons may be detected by increased fluorescence induced by polymerase-mediated 5′ nuclease cleavage or hybridization of a self-quenching probe complementary to both target and external control polynucleotides. A kit of PCR reagents can be dispensed into vessels for rapid and accurate nucleic acid amplification assay, with real-time or end-point measurements. The amplification control reagents, kits, and methods of the present invention provide positive and negative control tests which can be conducted concurrently with target amplification. Allelic differences at genetic loci can be detected, including single nucleotide polymorphisms (SNP).

    摘要翻译： 提供使用外部对照的核酸扩增方法，其验证特定靶序列的不存在或存在，以及校正引物和探针。 使用与靶引物相同序列的引物扩增单链外部对照多核苷酸。 具有可检测标记和具有针对和外部对照多核苷酸序列的序列的探针允许检测和测量。 当与外部对照多核苷酸杂交时，引物和可检测的探针相邻或基本相邻。 可以通过聚合酶介导的5'核酸酶切割诱导的增加的荧光或与靶和外部对照多核苷酸互补的自猝灭探针的杂交来检测靶和对照扩增子。 PCR试剂盒可以分配到容器中，用于快速准确的核酸扩增测定，具有实时或终点测量。 本发明的扩增控制试剂，试剂盒和方法提供可以与靶扩增同时进行的阳性和阴性对照试验。 可以检测遗传基因座中的等位基因差异，包括单核苷酸多态性（SNP）。