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公开(公告)号：US07501240B2
公开(公告)日：2009-03-10
申请号：US10573215
申请日：2004-12-01
CPC分类号： C07H21/00 , C12Q1/6827 , C12Q2527/125 , C12Q2523/125
摘要： The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.
摘要翻译： 本发明涉及检测核酸中的甲基化胞嘧啶，其中将亚硫酸氢胍用于制备含有胍离子和亚硫酸根离子的溶液以及随后的核酸修饰。 由此，将非甲基化胞嘧啶转化为尿嘧啶。 本发明还公开了用于执行本发明方法的套件。
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公开(公告)号：US20080281087A1
公开(公告)日：2008-11-13
申请号：US12095299
申请日：2006-12-12
CPC分类号： C07H21/00 , C12Q1/6806 , C12Q1/6827 , C12Q2565/518 , C12Q2527/125 , C12Q2523/125
摘要： The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines. In this method, the nucleic acid is bisulfite treated and is bound to a solid phase when an alkaline solution is added for desulfonation and elution of the nucleic acid from the solid phase. The nucleic acid can be amplified in an additional step. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, several uses of the alkaline solution for bisulfite treatment are disclosed and a kit containing a bisulfite reagent, a solid phase and an alkaline solution.
摘要翻译： 本申请涉及用于进行亚硫酸氢盐反应以确定核酸中甲基化位置的方法，即甲基化和非甲基化胞嘧啶。 在该方法中，当加入碱溶液用于脱磺化和从固相中洗脱核酸时，核酸是亚硫酸氢盐处理的并结合固相。 可以在额外的步骤中扩增核酸。 固相优选为包含玻璃或二氧化硅，更优选玻璃纤维，玻璃膜或磁性玻璃颗粒的材料。 此外，公开了碱性溶液用于亚硫酸氢盐处理的几种用途，以及含有亚硫酸氢盐试剂，固相和碱性溶液的试剂盒。
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3.发明授权Method, kit and apparatus comprising magnetic glass particles for the isolation of biomolecules 有权包括用于分离生物分子的磁性玻璃颗粒的方法，试剂盒和装置公开(公告)号：US06562568B1
公开(公告)日：2003-05-13
申请号：US09509750
申请日：2000-09-05
IPC分类号： C12Q168
CPC分类号： H01F1/112 , B03C1/01 , C03C14/004 , C07H1/08 , C12N15/1006 , C12N15/1013 , C12Q1/6806 , G01N35/0098 , G01N35/0099
摘要： The invention concerns a process for preparing biological samples for the subsequent detection of an analyte. In particular, the invention relates to a process for the isolation of a nucleic acid in a sample using a suspension of magnetic glass particles. In addition, kits and apparatuses containing magnetic glass particles for sample preparation are provided.
摘要翻译： 本发明涉及一种制备用于随后检测分析物的生物样品的方法。 特别地，本发明涉及使用磁性玻璃颗粒的悬浮液分离样品中核酸的方法。 另外，提供了包含用于样品制备的磁性玻璃颗粒的试剂盒和装置。
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公开(公告)号：US5573922A
公开(公告)日：1996-11-12
申请号：US235393
申请日：1994-04-29
IPC分类号： G01N33/53 , G01N33/545
CPC分类号： G01N33/5308 , Y10S436/815
摘要： An immunological method for the determination of triazine and triazine derivatives by a competitive immunoassay in which the sample and labelled atrazine compete for a polyclonal antibody which is bound to a solid phase before, during or after the immunological reaction, the immunological complex binds to the solid phase, the solid phase and unbound labelled atrazine are separated and the label in the solid or liquid phase is determined as a measure of the content of triazine and triazine derivatives, which is characterized in that polyclonal antibodies which were obtained by immunization with a 4-alkylamino-S-triazine are used and a conjugate of a label and 4-amino-S-triazine which is bound to the label via the 6 position is used as the labelled atrazine. Triazine and triazine derivatives can be determined simultaneously using this method.
摘要翻译： 通过竞争性免疫测定法测定三嗪和三嗪衍生物的免疫学方法，其中样品和标记的莠去津竞争在免疫反应之前，期间或之后与固相结合的多克隆抗体，免疫复合物结合固体 分离固相和未结合标记的莠去津，固定或液相中的标记作为三嗪和三嗪衍生物含量的量度来确定，其特征在于通过用4- 使用烷基氨基-S-三嗪，并且通过6位与标记结合的标记物和4-氨基-S-三嗪的缀合物用作标记的莠去津。 可以使用该方法同时测定三嗪和三嗪衍生物。
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公开(公告)号：US07413855B2
公开(公告)日：2008-08-19
申请号：US10540406
申请日：2004-01-28
CPC分类号： C07H21/04 , C01D5/145 , C12Q1/6827 , C12Q2527/101 , C12Q2523/125
摘要： The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is incubated in a solution comprising the nucleic acid for a time period of 1.5 to 3.5 hours at a temperature between 70 and 90° C., whereby the concentration of bisulfite in the solution is between 3 M and 6.25 M and whereby the pH value of the solution is between 5.0 and 6.0 whereby the nucleic acid, i.e. the cytosine bases in the nucleic acid, are deaminated. Then the solution comprising the deaminated nucleic acid is desulfonated and preferably desalted. The application is further related to a solution comprising bisulfite with a certain pH and uses thereof as well as a kit comprising the solution.
摘要翻译： 本申请涉及用于进行亚硫酸氢盐反应以确定核酸（即甲基化和非甲基化胞嘧啶）中的甲基化位置的方法，由此将核酸在包含核酸的溶液中温育1.5天至 在70〜90℃的温度下3.5小时，由此在该溶液中的亚硫酸氢盐浓度在3〜6.25M之间，由此溶液的pH值在5.0和6.0之间，由此核酸，即胞嘧啶碱基 在核酸中进行脱氨。 然后将包含脱氨酸核酸的溶液脱磺化并优选脱盐。 该应用还涉及包含具有一定pH的亚硫酸氢盐和其用途的溶液以及包含该溶液的试剂盒。
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6.发明申请Immune complex-specific antibodies for increased sensitivity in immunoassay array tests 有权免疫复合物特异性抗体，用于在免疫测定阵列测试中提高灵敏度公开(公告)号：US20060115907A1
公开(公告)日：2006-06-01
申请号：US11260938
申请日：2005-10-28
IPC分类号： G01N33/543
CPC分类号： G01N33/543 , G01N33/6854
摘要： The invention concerns a method for determining antigen-specific antibodies of a particular immunoglobulin class in a sample by means of an immunoassay in an array format in which various binding partners Bnx are bound on different discrete areas on a support where Bnx in each case contain the various antigens that are able to specifically bind to the antibodies to be detected, by incubating the support with the sample and a binding partner B2 which carries a label and subsequently detecting the label on the respective discrete areas wherein B2 specifically binds antibodies of a certain immunoglobulin class that have been bound in an antigen-specific manner.
摘要翻译： 本发明涉及一种通过以阵列形式的免疫测定来测定样品中特定免疫球蛋白类别的抗原特异性抗体的方法，其中各种结合配偶体B nxx结合在不同的离散区域上 支持，其中每种情况下，B 含有能够特异性结合待检测的抗体的各种抗原，通过将载体与样品和结合配偶体B 2孵育 其携带标签并随后检测相应离散区域上的标记，其中B 2特异性结合以抗原特异性方式结合的某一免疫球蛋白类别的抗体。
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公开(公告)号：US08129106B2
公开(公告)日：2012-03-06
申请号：US11570672
申请日：2005-06-14
CPC分类号： C07H19/16 , C12Q1/6827 , C12Q2537/164 , C12Q2522/101 , C12Q2521/125
摘要： A method for detecting sequence specific methylation in a biomolecule, comprising: (a) contacting the biomolecule with an S-adenosyl-L-methionine-dependent methyltransferase in the presence of a detectable cofactor of said methyltransferase; and (b) detecting whether the recognition sequence of said methyltransferase has been modified with the cofactor or a derivative thereof, wherein modification of the recognition sequence of said methyltransferase is indicative of an absence of methylation at said recognition sequence. Also disclosed is a cofactor specific for S-adenosyl-L-methionine-dependent methyltransferases, wherein said cofactor is an N-adenosylaziridine derivative with a reporter group attached to the 6 or 7 position of the adenine ring or attached to the aziridine ring. A complex of the cofactor and a methyltransferase a composition comprising the cofactor or the complex and the use of the cofactor or the complex for detecting sequence-specific methylation in DNA molecules are also disclosed.
摘要翻译： 一种用于检测生物分子中序列特异性甲基化的方法，包括：（a）在所述甲基转移酶的可检测辅因子的存在下，使所述生物分子与S-腺苷-L-甲硫氨酸依赖性甲基转移酶接触; 和（b）检测所述甲基转移酶的识别序列是否已经用辅因子或其衍生物修饰，其中所述甲基转移酶的识别序列的修饰指示在所述识别序列处不存在甲基化。 还公开了对S-腺苷-L-甲硫氨酸依赖性甲基转移酶特异性的辅因子，其中所述辅因子是N-腺苷氮丙啶衍生物，其报告基团连接到腺嘌呤环的6或7位或连接到氮丙啶环上。 还公开了辅因子和甲基转移酶的复合物，其包含辅因子或复合物以及辅因子或复合物用于检测DNA分子中的序列特异性甲基化的组合物。
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公开(公告)号：US20110165564A1
公开(公告)日：2011-07-07
申请号：US11570672
申请日：2005-06-14
CPC分类号： C07H19/16 , C12Q1/6827 , C12Q2537/164 , C12Q2522/101 , C12Q2521/125
摘要： A method for detecting sequence specific methylation in a biomolecule, comprising: (a) contacting the biomolecule with an S-adenosyl-L-methionine-dependent methyltransferase in the presence of a detectable cofactor of said methyltransferase; and (b) detecting whether the recognition sequence of said methyltransferase has been modified with the cofactor or a derivative thereof, wherein modification of the recognition sequence of said methyltransferase is indicative of an absence of methylation at said recognition sequence. Also disclosed is a cofactor specific for S-adenosyl-L-methionine-dependent methyltransferases, wherein said cofactor is an N-adenosylaziridine derivative with a reporter group attached to the 6 or 7 position of the adenine ring or attached to the aziridine ring. A complex of the cofactor and a methyltransferase a composition comprising the cofactor or the complex and the use of the cofactor or the complex for detecting sequence-specific methylation in DNA molecules are also disclosed.
摘要翻译： 一种用于检测生物分子中序列特异性甲基化的方法，包括：（a）在所述甲基转移酶的可检测辅因子的存在下，使所述生物分子与S-腺苷-L-甲硫氨酸依赖性甲基转移酶接触; 和（b）检测所述甲基转移酶的识别序列是否已经用辅因子或其衍生物修饰，其中所述甲基转移酶的识别序列的修饰指示在所述识别序列处不存在甲基化。 还公开了对S-腺苷-L-甲硫氨酸依赖性甲基转移酶特异性的辅因子，其中所述辅因子是N-腺苷氮丙啶衍生物，其报告基团连接到腺嘌呤环的6或7位或连接到氮丙啶环上。 还公开了辅因子和甲基转移酶的复合物，其包含辅因子或复合物以及辅因子或复合物用于检测DNA分子中的序列特异性甲基化的组合物。
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公开(公告)号：US20090208930A1
公开(公告)日：2009-08-20
申请号：US11658569
申请日：2005-09-02
CPC分类号： C12Q1/6848 , C12Q1/6858 , C12Q2523/125 , C12Q2521/331
摘要： The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention.
摘要翻译： 本发明涉及一种用于扩增样品中的甲基化靶核酸的方法，同时避免通过使其失活来扩增非甲基化靶核酸。 这是通过目标核酸亚硫酸氢盐处理后的限制酶消化完成的。 本发明进一步涉及使用限制酶避免扩增未甲基化的靶核酸同时扩增样品中的甲基化靶核酸和用于实施根据本发明的方法的试剂盒。
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公开(公告)号：US20070190530A1
公开(公告)日：2007-08-16
申请号：US10573215
申请日：2004-12-01
CPC分类号： C07H21/00 , C12Q1/6827 , C12Q2527/125 , C12Q2523/125
摘要： The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.
摘要翻译： 本发明涉及检测核酸中的甲基化胞嘧啶，其中将亚硫酸氢胍用于制备含有胍离子和亚硫酸根离子的溶液，随后改变核酸。 由此，将非甲基化胞嘧啶转化为尿嘧啶。 本发明还公开了用于执行本发明方法的套件。
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