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公开(公告)号：US07192735B2
公开(公告)日：2007-03-20
申请号：US10261049
申请日：2002-09-30
IPC分类号： C12P21/02 , C12P1/00 , C12P39/00 , C12N9/10 , C12N1/19 , C12N1/21 , C12N5/04 , C12N5/06 , A61K38/04 , C07H21/04
CPC分类号： C12N9/1258 , C12N9/1029 , C12Q1/48 , G01N33/5005 , G01N33/569
摘要： The invention pertains to isolated phosphopantetheinyl transferases, such as the E. coli acyl carrier protein synthase, which transfer a phosphopantetheinyl group onto a substrate. The enzyme can be purified from a natural source, produced recombinantly, or synthetically. Accordingly, the invention provides compositions and kits including phosphopantetheinyl transferases and host cells expressing phosphopantetheinyl transferases. The invention also provides nucleic acids encoding phosphopantetheinyl transferases and vectors comprising such nucleic acids. The invention further provides methods for phosphopantetheinylating a substrate in vitro or in vivo and methods for producing antibiotics in vitro or in vivo.
摘要翻译： 本发明涉及分离的磷酸二氢乙烯基转移酶，例如大肠杆菌酰基载体蛋白合成酶，其将磷酸泛酰乙烯基转移到基底上。 该酶可以从天然来源纯化，重组产生或合成产生。 因此，本发明提供了组合物和试剂盒，其包括磷酸泛酰乙酰转移酶和表达磷酸聚乙二炔转移酶的宿主细胞。 本发明还提供了编码磷酸泛酰乙酰转移酶的核酸和包含此类核酸的载体。 本发明进一步提供了体外或体内磷酸化乙酰化底物的方法以及在体外或体内生产抗生素的方法。
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公开(公告)号：US4758195A
公开(公告)日：1988-07-19
申请号：US904299
申请日：1986-09-08
申请人： Christopher T. Walsh
发明人： Christopher T. Walsh
CPC分类号： A63H33/04
摘要： Elastomeric foam blocks are provided for constructing toys and models with a means of attachment of one block to another block in the form of flexible protuberances and cellular receptacles present on all surfaces, so that when one or more blocks are engaged, a clinging force is generated. Furthermore, either independently of, or in collaboration with the above, another means of connecting said blocks is provided by inserting simple pins into the matrix of said foam in any direction and without preformed perforations. The pins are held in place in the perforations created by the insertion of the pins into the foam by the elastomeric forces inherent in the polymeric structure of the foam, and the perforations snap shut upon the removal of the pins, thus allowing the building units to be reused again to construct more toys and models.
摘要翻译： 弹性体泡沫块被提供用于构造玩具和模型，其具有将一个块附接到另一个块的方式，其以存在于所有表面上的柔性突起和细胞容器的形式存在，使得当一个或多个块被接合时，产生附着力 。 此外，独立于或与上述协作，通过在任何方向上将简单的销插入所述泡沫的基体中并且没有预先形成的穿孔来提供连接所述块的另一种装置。 销被保持在通过由泡沫聚合物结构中固有的弹性力插入泡沫而产生的穿孔中，并且穿孔在销的移除时咬合，从而允许建筑物单元 再次重新使用更多的玩具和模型。
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公开(公告)号：US06579695B1
公开(公告)日：2003-06-17
申请号：US08728742
申请日：1996-10-11
IPC分类号： C12P1926
CPC分类号： C12N9/1258 , C12N9/1029 , C12Q1/48 , G01N33/5005 , G01N33/569
摘要： The invention pertains to isolated phosphopantetheinyl transferases, such as the E. coli acyl carrier protein synthase, which transfer a phosphopantetheinyl group onto a substrate. The enzyme can be purified from a natural source, produced recombinantly, or synthetically. Accordingly, the invention provides compositions and kits including phosphopantetheinyl transferases and host cells expressing phosphopantetheinyl transferases. The invention also provides nucleic acids encoding phosphopantetheinyl transferases and vectors comprising such nucleic acids. The invention further provides methods for phosphopantetheinylating a substrate in vitro or in vivo and methods for producing antibiotics in vitro or in vivo.
摘要翻译： 本发明涉及分离的磷酸二氢乙烯基转移酶，例如大肠杆菌酰基载体蛋白质合酶，其将磷酸泛酰乙烯基转移到基底上。 该酶可以从天然来源纯化，重组产生或合成产生。 因此，本发明提供了组合物和试剂盒，其包括磷酸泛酰乙酰转移酶和表达磷酸聚乙二炔转移酶的宿主细胞。 本发明还提供了编码磷酸泛酰乙酰转移酶的核酸和包含此类核酸的载体。 本发明进一步提供了体外或体内磷酸化乙酰化底物的方法以及在体外或体内生产抗生素的方法。
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公开(公告)号：US20100048422A1
公开(公告)日：2010-02-25
申请号：US11989257
申请日：2006-07-21
CPC分类号： C12N15/102 , C12N15/1093 , C12N15/52
摘要： The present invention provides a method producing a modified assembly line, such as those that produce non-ribosomal peptides and polyketides. The modified assembly lines of the invention can be used to produce novel compounds with therapeutic activities. The invention also provides organisms containing modified assembly lines and libraries of modified assembly lines.
摘要翻译： 本发明提供了生产改性装配线的方法，例如产生非核糖体肽和聚酮化合物的那些方法。 本发明的改性装配线可用于生产具有治疗活性的新型化合物。 本发明还提供了包含改进的组装线的生物体和改进的装配线的文库。
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公开(公告)号：US5648244A
公开(公告)日：1997-07-15
申请号：US127692
申请日：1993-09-27
CPC分类号： C12N9/644 , C07K14/39 , C12N15/62 , C12N9/90 , C12Y304/21022 , C07K2319/00 , C07K2319/23 , C07K2319/75
摘要： A method for producing a fusion peptide. A vector is provided with nucleic acid encoding a carrier peptide and at the 3' end of the nucleic acid, a unidirectional restriction endonuclease cleavage site recognized by a restriction endonuclease with the ability to create a non-palindromic 3-base overhang. The vector is cleaved with the restriction endonuclease to produce a cleaved vector. One or more nucleic acids encoding a desired peptide and having at least a 3-base overhang at each end configured and arranged for ligation with the cleaved vector is then ligated to the cleavage site.
摘要翻译： 一种融合肽的制备方法。 向载体提供编码载体肽的核酸，并且在核酸的3'末端，由限制性内切核酸酶识别的单向限制性内切核酸酶切割位点具有产生非回文性3-碱基突出端的能力。 用限制性内切核酸酶切割载体以产生切割的载体。 编码所需肽并且在每个末端具有至少3个碱基突出端的一个或多个核酸被配置并排列用于与切割的载体连接，然后连接到切割位点。
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