公开(公告)号：US20090022698A1

公开(公告)日：2009-01-22

申请号：US12237207

申请日：2008-09-24

申请人： Chul-Won Ha ,  Yoon-Sun Yang ,  Sung-Eun Yang

发明人： Chul-Won Ha ,  Yoon-Sun Yang ,  Sung-Eun Yang

IPC分类号： A61K35/12 ,  C12N5/06 ,  A61P19/00

CPC分类号： A61K35/28 ,  A61K35/51 ,  Y02A50/411

摘要： Disclosed is a composition for the treatment of cartilage or bone damage or loss or defect. The composition comprises mesenchymal stem cells separated from umbilical cord blood and/or mesenchymal stem cells proliferated and/or differentiated. The composition also comprise chondrocytes and/or chondroblasts, or osteocytes and/or osteoblasts, differentiated from the mesenchymal stem cells separated from the umbilical cord blood.

公开(公告)号：US20100184218A1

公开(公告)日：2010-07-22

申请号：US12579140

申请日：2009-10-14

申请人： Chul-Won HA ,  Yoon-Sun Yang ,  Sung-Eun Yang

发明人： Chul-Won HA ,  Yoon-Sun Yang ,  Sung-Eun Yang

IPC分类号： C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0665 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/42 ,  C12N2501/15

摘要： There is provided a method for the isolation and cultivation of mesenchymal stem/progenitor cells from umbilical cord blood, and also to a method for the differentiation of the umbilical cord blood-derived mesenchymal stem/progenitor cells into various mesenchymal tissues. The method include overlaying umbilical cord blood onto Ficoll-Hypaque solution; centrifuging the umbilical cord blood on the Ficoll-Hypaque solution to obtain a mononuclear cell layer; reacting cells obtained by monolayer culture of the mononuclear cells with antibodies to mesenchymal stem/progenitor cell-specific antigens for a predetermined period of incubation time; isolating only cells bound to their corresponding antibodies using a cell sorter; and cultivating the isolated cells, thereby obtaining mesenchymal stem/progenitor cells with high purity and excellent viability.

摘要翻译： 提供了从脐带血分离和培养间充质干/祖细胞的方法，以及将脐带血来源的间充质干/祖细胞分化成各种间充质组织的方法。 该方法包括将脐带血覆盖在Ficoll-Hypaque溶液上; 在Ficoll-Hypaque溶液上离心脐带血，获得单核细胞层; 将单核细胞的单层培养获得的细胞与间充质干/祖细胞特异性抗原的抗体反应预定的孵育时间; 使用细胞分选器分离仅与其相应抗体结合的细胞; 并培养分离的细胞，从而获得高纯度和优良活力的间充质干细胞/祖细胞。

公开(公告)号：US20090060885A1

公开(公告)日：2009-03-05

申请号：US12237182

申请日：2008-09-24

申请人： Chul-Won Ha ,  Yoon-Sun Yang ,  Sung-Eun Yang

发明人： Chul-Won Ha ,  Yoon-Sun Yang ,  Sung-Eun Yang

IPC分类号： A61K35/12 ,  A61P19/04

CPC分类号： A61K35/28 ,  A61K35/51 ,  Y02A50/411

摘要： Disclosed is a composition for the treatment of cartilage or bone damage or loss or defect. The composition comprises mesenchymal stem cells separated from umbilical cord blood and/or mesenchymal stem cells proliferated and/or differentiated. The composition also comprise chondrocytes and/or chondroblasts, or osteocytes and/or osteoblasts, differentiated from the mesenchymal stem cells separated from the umbilical cord blood.

摘要翻译： 公开了用于治疗软骨或骨损伤或损失或缺陷的组合物。 该组合物包含从脐带血和/或间充质干细胞分离的间充质干细胞增殖和/或分化。 组合物还包括与从脐带血分离的间充质干细胞分化的软骨细胞和/或软骨细胞，或骨细胞和/或成骨细胞。

公开(公告)号：US20090047261A1

公开(公告)日：2009-02-19

申请号：US12237225

申请日：2008-09-24

申请人： Chul-Won HA ,  Yoon-sun Yang ,  Sung-Eun Yang

发明人： Chul-Won HA ,  Yoon-sun Yang ,  Sung-Eun Yang

IPC分类号： A61K35/12

CPC分类号： A61K35/28 ,  A61K35/51 ,  Y02A50/411

摘要： Disclosed is a composition for the treatment of cartilage or bone damage or loss or defect. The composition comprises mesenchymal stem cells separated from umbilical cord blood and/or mesenchymal stem cells proliferated and/or differentiated. The composition also comprise chondrocytes and/or chondroblasts, or osteocytes and/or osteoblasts, differentiated from the mesenchymal stem cells separated from the umbilical cord blood.

摘要翻译： 公开了用于治疗软骨或骨损伤或损失或缺陷的组合物。 该组合物包含从脐带血和/或间充质干细胞分离的间充质干细胞增殖和/或分化。 组合物还包括与从脐带血分离的间充质干细胞分化的软骨细胞和/或软骨细胞，或骨细胞和/或成骨细胞。

公开(公告)号：US07459307B2

公开(公告)日：2008-12-02

申请号：US10485816

申请日：2002-08-14

申请人： Chul-Won Ha ,  Yoon-Sun Yang ,  Sung-Eun Yang

发明人： Chul-Won Ha ,  Yoon-Sun Yang ,  Sung-Eun Yang

IPC分类号： C12N5/00 ,  A01N63/00

CPC分类号： A61K35/28 ,  A61K35/51 ,  Y02A50/411

摘要： Disclosed herein is a composition for the treatment of articular cartilage damage or loss or defect. The composition for the treatment of articular cartilage injury of the present invention includes (i) cellular components separated, proliferated, and/or differentiated from the umbilical cord blood, (ii) a culture medium; and (iii) a biocompatible polymer. The composition has very superior ability of proliferation and differentiation and easier to be collected and acquired.

摘要翻译： 本文公开了用于治疗关节软骨损伤或损失或缺陷的组合物。 用于治疗本发明的关节软骨损伤的组合物包括（i）从脐带血分离，增殖和/或分化的细胞成分，（ii）培养基; 和（iii）生物相容性聚合物。 该组合物具有非常优异的增殖和分化能力，更易于收集和获得。

公开(公告)号：US20050118714A1

公开(公告)日：2005-06-02

申请号：US10503134

申请日：2003-02-19

申请人： Chul-Won Ha ,  Yoon-Sun Yang ,  Sung-Eun Yang

发明人： Chul-Won Ha ,  Yoon-Sun Yang ,  Sung-Eun Yang

IPC分类号： C12Q1/68 ,  A61L27/00 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/08

CPC分类号： C12N5/0665 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/42 ,  C12N2501/15

摘要： The present invention relates to a method for the isolation and cultivation of mesenchymal stem/progenitor cells from umbilical cord blood, and also to a method for the differentiation of the umbilical cord blood-derived mesenchymal stem/progenitor cells into various mesenchymal tissues. The method comprises the steps of: overlaying umbilical cord blood onto Ficoll-Hypaque solution; centrifuging the umbilical cord blood on the Ficoll-Hypaque solution to obtain a mononuclear cell layer; reacting cells obtained by monolayer culture of the mononuclear cells with antibodies to mesenchymal stem/progenitor cell-specific antigens for a predetermined period of incubation time; isolating only cells bound to their corresponding antibodies using a cell sorter; and cultivating the isolated cells, thereby obtaining mesenchymal stem/progenitor cells with high purity and excellent viability. The mesenchymal stem/progenitor cells of the present invention are capable of differentiating into various mesenchymal tissues including chondrocytes and osteoblasts. Thus, the method of cell isolation and cultivation according to the present invention allows mass production of the mesenchymal stem/progenitor cells, and the cells obtained by the present invention are useful in the renewal and treatment of injured mesenchymal tissues.

摘要翻译： 本发明涉及从脐带血分离和培养间充质干细胞/祖细胞的方法，还涉及将脐带血来源的间充质干细胞/祖细胞分化为各种间充质组织的方法。 该方法包括以下步骤：将脐带血重叠在Ficoll-Hypaque溶液上; 在Ficoll-Hypaque溶液上离心脐带血，获得单核细胞层; 将单核细胞的单层培养获得的细胞与间充质干/祖细胞特异性抗原的抗体反应预定的孵育时间; 使用细胞分选器分离仅与其相应抗体结合的细胞; 并培养分离的细胞，从而获得高纯度和优良活力的间充质干细胞/祖细胞。 本发明的间充质干/祖细胞能够分化为包括软骨细胞和成骨细胞在内的各种间充质组织。 因此，根据本发明的细胞分离和培养方法允许大量生产间充质干细胞/祖细胞，并且通过本发明获得的细胞可用于损伤的间充质组织的更新和治疗。