公开(公告)号：US5372820A

公开(公告)日：1994-12-13

申请号：US961033

申请日：1992-10-14

申请人： Jacqueline Jozefonvicz nee Dorgebray ,  Xavier-Francois Santarelli ,  Daniel A. Muller

发明人： Jacqueline Jozefonvicz nee Dorgebray ,  Xavier-Francois Santarelli ,  Daniel A. Muller

IPC分类号： G01N33/543 ,  B01J20/281 ,  B01J20/32 ,  G01N30/88 ,  C12N11/00 ,  C07K17/00

CPC分类号： B01J20/24 ,  B01J20/103 ,  B01J20/262 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/28061 ,  B01J20/28069 ,  B01J20/28078 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3092 ,  B01J20/3204 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3221 ,  B01J20/3274 ,  B01J20/328 ,  B01J20/3282 ,  B01J20/3289 ,  B01J2220/4812 ,  B01J2220/4843 ,  B01J2220/54 ,  B01J2220/58 ,  Y10S530/813 ,  Y10S530/814

摘要： This granular material consists of a granular porous support bearing negative or positive charges, coated on its surface with a first layer of impregnation with a hydrophilic polymer bearing charges opposite to those of the support, the quantity of said charges present on said polymer being substantially equal to that of the charges present at the surface of the support; and another layer, bound to the first by irreversible chemical coupling, of another hydrophilic polymer which is functional, namely bearing groups endowing said functional hydrophilic polymer with a specific but reversible affinity for at least one biological substance. Among the applications of this material, there are mentioned the separation and purification of antithrombin III and coagulation factors (factors II, VII, VIII, IX, X), and separation of activated coagulation factors (activated factor II, activated factor IX, activated factor X) in solutions containing said non-activated factors.

摘要翻译： 这种粒状材料由承载负电荷或正电荷的颗粒状多孔载体构成，在其表面上涂覆有第一层浸渍，其中亲水聚合物带有与载体相反的电荷，所述聚合物上存在的所述电荷的量基本相等 与支持物表面的指控相关; 另一个与第一个不可逆化学偶联的另一层是另一种功能性的亲水性聚合物，即承载基团赋予所述官能亲水性聚合物对至少一种生物物质具有特异但可逆的亲和力。 在这种材料的应用中，提到了抗凝血酶III和凝血因子（因子II，VII，VIII，IX，X）的分离和纯化以及活化凝血因子（活化因子II，活化因子IX，活化因子 X）在含有所述非活化因子的溶液中。

公开(公告)号：US5019269A

公开(公告)日：1991-05-28

申请号：US497560

申请日：1990-03-21

申请人： Didier Letourneur ,  Colette Douzon ,  Veronique Migonney ,  Daniel A. Muller ,  Marcel Jozefowicz

发明人： Didier Letourneur ,  Colette Douzon ,  Veronique Migonney ,  Daniel A. Muller ,  Marcel Jozefowicz

IPC分类号： A61M1/36 ,  B01J20/26 ,  B01J39/12 ,  C08B37/02 ,  C08F8/30 ,  C08F8/34 ,  C08F8/40 ,  C08L25/00 ,  G01N33/545 ,  G01N33/86

CPC分类号： G01N33/86 ,  A61M1/3679 ,  B01J20/26 ,  B01J39/12 ,  C08B37/0021 ,  C08F8/30 ,  C08F8/34 ,  C08F8/40 ,  G01N33/545

摘要： These resins derived from a crosslinked styrene (co)-polymer, or from a crosslinked dextran, comprise a (co)-polymer chain which is substituted with one or more groups, which may be identical or different, belonging to the following categories: --Z--A.sub.1 ; --Z--A.sub.2 ; --Z--A.sub.1 --Z'--A.sub.2 ; --Z--A.sub.1 --A.sub.3 --A.sub.2 ; --Z--A.sub.1 --A.sub.4, where Z=spacer chain, Z'=linking chain, A.sub.1 =phosphate residue, A.sub.2 =residue of purine or pyrimidine base, A.sub.3 =sugar residue and A.sub.4 =residue of a molecule participating in the polar structure of various phospholipids. These resins are applicable for the analysis and purification of molecules of biological origin, in particular as a stationary phase in ion-exchange and affinity chromatography, especially for carrying out the fractionation of protein mixtures. Their interaction with proteins, in particular the anti-DNA and anti-phospholipid antibodies present in the serum of patients suffering from SLE, makes them applicable to operations for the selective purification of the different types of antibodies developed by Lupus patients.

公开(公告)号：US4950712A

公开(公告)日：1990-08-21

申请号：US235113

申请日：1988-08-22

申请人： Didier Letourneur ,  Colette Douzon ,  Veronique Migonney ,  Daniel A. Muller ,  Marcel Jozefowicz

发明人： Didier Letourneur ,  Colette Douzon ,  Veronique Migonney ,  Daniel A. Muller ,  Marcel Jozefowicz

IPC分类号： A61M1/36 ,  B01J20/26 ,  B01J39/12 ,  C08B37/02 ,  C08F8/30 ,  C08F8/34 ,  C08F8/40 ,  C08L25/00 ,  G01N33/545 ,  G01N33/86

CPC分类号： G01N33/86 ,  A61M1/3679 ,  B01J20/26 ,  B01J39/12 ,  C08B37/0021 ,  C08F8/30 ,  C08F8/34 ,  C08F8/40 ,  G01N33/545

摘要： These resins derived from a crosslinked styrene (co)-polymer, or from a crosslinked dextran, comprise a (co)-polymer chain which is substituted with one or more groups, which may be identical or different, belonging to the following categories: --Z--A.sub.1 ; --Z--A.sub.2 ; --Z--A.sub.1 --Z'--A.sub.2 ; --Z--A.sub.1 --A.sub.3 --A.sub.2 ; --Z--A.sub.1 --A.sub.4, where Z=spacer chain, Z'=linking chain, A.sub.1 =phosphate residue, A.sub.2 =residue of purine or pyrimidine base, A.sub.3 =sugar residue and A.sub.4 =residue of a molecule participating in the polar structure of various phospholipids. These resins are applicable for the analysis and purification of molecules of biological origin, in particular as a stationary phase in ion-exchange and affinity chromatography, especially for carrying out the fractionation of protein mixtures. Their interaction with proteins, in particular the anti-DNA and anti-phospholipid antibodies present in the serum of patients suffering from SLE, makes them applicable to operations for the selective purification of the different types of antibodies developed by lupus patients.

摘要翻译： 衍生自交联苯乙烯（共）聚合物或交联葡聚糖的这些树脂包含被一个或多个可以相同或不同的基团取代的（共） - 聚合物链，属于以下类别： Z-A1; -Z-A2; -Z-A1-Z'-A2; -Z-A1-A3-A2; -Z-A1-A4，其中Z =间隔链，Z'=连接链，A1 =磷酸酯残基，A2 =嘌呤或嘧啶碱基的残基，A3 =糖残基，A4 =参与极性结构的分子的残基 各种磷脂。 这些树脂适用于分离和纯化生物来源的分子，特别是作为离子交换和亲和层析中的固定相，特别是用于进行蛋白质混合物的分级分离。 它们与蛋白质的相互作用，特别是存在于患有SLE的患者的血清中的抗DNA和抗磷脂抗体，使得它们适用于由狼疮患者开发的不同类型的抗体的选择性纯化的操作。