公开(公告)号：US12043853B2

公开(公告)日：2024-07-23

申请号：US17376669

申请日：2021-07-15

申请人： DANISCO US INC

发明人： Andrew Shaw ,  David A Estell ,  Eugene S Sadlowski ,  Hiroshi Oh ,  Louise Wallace ,  Ronaldus Wilhelmus Joannes Hommes ,  Sang-Kyu Lee

IPC分类号： C12N9/54 ,  C11D1/02 ,  C11D3/386

CPC分类号： C12N9/54 ,  C11D1/02 ,  C11D3/386 ,  C11D3/38663 ,  C12Y304/24

摘要： The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

公开(公告)号：US12012604B2

公开(公告)日：2024-06-18

申请号：US17347204

申请日：2021-06-14

申请人： DANISCO US INC.

发明人： Paul Klaassen ,  Jozef Petrus Johannes Schmitz ,  Paulus Petrus De Waal ,  Arjen Bouma

IPC分类号： C12N15/81 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/12 ,  C12N9/16 ,  C12N9/92 ,  C12P7/06 ,  C12P7/10

CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/92 ,  C12N9/93 ,  C12P7/06 ,  C12P7/10 ,  C12Y102/0101 ,  C12Y207/01028 ,  C12Y207/01029 ,  C12Y301/03021 ,  C12Y503/01005 ,  C12Y602/01001 ,  C12P2203/00 ,  Y02E50/10

摘要： The present invention relates to a yeast cell that is genetically modified comprising:



a) a disruption of one or more aldehyde dehydrogenase (E.C:1.2.1.4) native to the yeast;
b) one or more nucleotide sequence encoding a heterologous NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10);
c) one or more nucleotide sequence encoding a homologous or heterologous acetyl-CoA synthetase (E.C. 6.2.1.1); and
d) a modification that leads to reduction of glycerol 3-phosphate phosphohydrolase (E.C. 3.1.3.21) and/or glycerol 3-phosphate dehydrogenase (E.C. 1.1.1.8 or E.C. 1.1.5.3) activity, native to the yeast.

公开(公告)号：US11946078B2

公开(公告)日：2024-04-02

申请号：US17237235

申请日：2021-04-22

申请人： DANISCO US INC

发明人： Neeraj Pandey ,  Sina Pricelius ,  Annapurna Sachan ,  Stepan Shipovskov ,  Richard Bott ,  Ajit Kumar Satapathy

IPC分类号： C12N9/24 ,  C11D3/386 ,  C12N9/42 ,  C12N15/63

CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12N9/24 ,  C12N15/63 ,  C12Y302/01004

摘要： Disclosed herein are cellulase variants, or active fragments thereof, and polynucleotides encoding same, where the cellulase variants, or active fragments thereof, have endoglucanase activity. Also disclosed herein are compositions comprising the cellulase variants, or active fragments thereof; vectors and/or host cells comprising the polynucleotides encoding the cellulase variants, or active fragments thereof; and methods for making and/or using the cellulase variants, or active fragments thereof and/or compositions containing same; where the cellulase variants, or active fragments thereof, have endoglucanase activity.

公开(公告)号：US20230365947A1

公开(公告)日：2023-11-16

申请号：US18044250

申请日：2021-09-16

申请人： Danisco US Inc.

发明人： FENG GUO ,  HELONG HAO ,  BRADLEY KELEMEN ,  AMY DEMING LIU ,  KEFENG NI ,  ZHEN QIAN ,  ZHONGMEI TANG

IPC分类号： C12N9/18 ,  C08J11/10

CPC分类号： C12N9/18 ,  C08J11/105 ,  C08J2367/02 ,  C08J2301/32

摘要： The present disclosure relates to a method and a composition comprising an esterase for enzymatic surface modification of a polyester. The present disclosure also relates to a method of degradation or hydrolysis of an insoluble plant material.

公开(公告)号：US20230348879A1

公开(公告)日：2023-11-02

申请号：US17901168

申请日：2022-09-01

申请人： DANISCO US INC

发明人： LUIS G CASCAO-PEREIRA ,  DINA FINAN ,  DAVID EDWARD WILDES ,  PIETER AUGUSTINUS ,  ROEL HERMANT ,  MONICA OCHA RUIZ ,  DEWY VAN TOL ,  RICHARD R. BOTT ,  MARC KOLKMAN

IPC分类号： C12N9/26 ,  C11D3/386 ,  C12N9/28 ,  A23L2/38 ,  C12P19/02 ,  C12P19/14

CPC分类号： C12N9/2414 ,  A23L2/382 ,  C11D3/386 ,  C11D3/38681 ,  C12N9/2417 ,  C12P19/02 ,  C12P19/14 ,  A23V2002/00

摘要： Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.

公开(公告)号：US20220389372A1

公开(公告)日：2022-12-08

申请号：US17774437

申请日：2020-11-10

申请人： DANISCO US INC.

发明人： Cristina Bongiorni ,  Zhen Ma ,  Anita Van Kimmenade

IPC分类号： C12N1/20 ,  C12N15/00 ,  C12N1/08 ,  C07K14/32

摘要： The present disclosure is generally related to compositions and methods for constructing and obtaining Bacillus sp. host cells (strains) having enhanced protein production phenotypes. Certain embodiments of the disclosure are therefore related to genetically modified Bacillus sp. cells derived (obtained) from parental Bacillus sp. cells (e.g., such as parental Bacillus cells expressing/producing a protein of interest). Thus, certain embodiments are directed to modified Bacillus sp. cells expressing/producing an endogenous protein of interest, or a heterologous protein of interest, wherein the modified Bacillus sp. cell comprises a genetic modification of a yitMOP operon, a combined genetic modification of a yitMOP operon and a sdpABC operon, a genetic modification of a yitM gene and the like, wherein the modified Bacillus sp. cells produce an increased amount of the POI relative the parental cells from which they were derived (i.e., when grown/cultivated/fermented under identical conditions).

公开(公告)号：US11473075B2

公开(公告)日：2022-10-18

申请号：US16720106

申请日：2019-12-19

申请人： DANISCO US INC

发明人： David A. Estell ,  Frits Goedegebuur ,  Marc Anton Bernhard Kolkman ,  Rie Mejldal ,  Katherine Augustyn ,  Lilia Maria Babe ,  Richard R Bott ,  Jian Yao ,  Roopa Santosh Ghirnikar

IPC分类号： C12N9/54 ,  C11D3/386

摘要： The present disclosure provides AprL-clade protease enzymes, including variant AprL-clade protease enzymes, nucleic acids encoding same, and compositions and methods related to the production and use thereof, including an AprL-clade variant subtilisin enzyme that has improved stability and/or soil removal compared to a parent AprL-clade subtilisin enzyme.

公开(公告)号：US11447782B2

公开(公告)日：2022-09-20

申请号：US16081082

申请日：2017-03-06

申请人： DANISCO US INC.

发明人： Cristina Bongiorni ,  Marc Kolkman ,  Chris Leeflang ,  Virgil Arthur Rhodius ,  Anita Van Kimmenade

IPC分类号： C07H21/04 ,  C12N15/75 ,  C12N9/28 ,  C07K14/32 ,  C12N9/54

摘要： The instant disclosure is generally related to novel engineered (hybrid) promoters. In certain embodiments, the instant disclosure is directed to one or more nucleic acid compositions comprising engineered promoters operably linked to nucleic acids encoding proteins of interest. Thus, the disclosure set forth herein described methods and compositions for the production of proteins of interest using one or more novel engineered (hybrid) promoters of the disclosure.

公开(公告)号：US20220282234A1

公开(公告)日：2022-09-08

申请号：US17635349

申请日：2020-08-10

申请人： DANISCO US INC.

发明人： Ryan L. FRISCH ,  Hongxian HE

IPC分类号： C12N9/28 ,  C12N1/20 ,  C12N15/11 ,  C12N9/22 ,  C12N15/75 ,  C07K14/32

摘要： The present disclosure is generally related to compositions and methods for constructing and obtaining Bacillus licheniformis cells having increased protein production phenotypes. Thus, certain embodiments are related to modified B. licheniformis cells derived from parental B. licheniformis cells. Certain embodiments are related to modified B. licheniformis cells comprising a modified rghR locus. Certain embodiments are related to modified B. licheniformis cells having a modified rghR locus and comprising an increased protein productivity phenotype. In certain other embodiments, modified B. licheniformis cells having a modified rghR locus produce a reduced amount of red pigment. In certain other embodiments, modified B. licheniformis cells comprise an increased protein productivity phenotype and produce a reduced amount of red pigment.

公开(公告)号：US11414643B2

公开(公告)日：2022-08-16

申请号：US16957217

申请日：2018-12-12

申请人： DANISCO US INC.

发明人： Shannon Del Chase ,  Carol Marie Fioresi ,  Ryan L. Frisch ,  Helen Olivia Masson ,  Anita Van Kimmenade

IPC分类号： C12N1/20 ,  C07K14/32 ,  C12N15/75 ,  C12P21/02

摘要： The instant disclosure is generally related to novel Bacillus sp. mutants capable of producing increased amounts of industrially relevant proteins of interest. Certain embodiments of the disclosure are related to modified Bacillus sp. cells comprising an introduced polynucleotide encoding a variant GlcT protein. Other embodiments are related to methods and compositions for producing endogenous and/or heterologous proteins of interest in the modified Bacillus sp. (daughter) cells, whereas certain other embodiments are directed to nucleic acid sequences, particularly polynucleotide open reading frame (ORF) sequences, vectors thereof and DNA expression constructs thereof, encoding variant GlcT proteins of the disclosure.