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公开(公告)号：US20240352499A1
公开(公告)日：2024-10-24
申请号：US18683749
申请日：2022-08-19
申请人： DANISCO US INC.
CPC分类号： C12P21/02 , C12N1/08 , C12N9/22 , C12N15/75 , C12R2001/125
摘要： The instant disclosure provides, among other things, novel nuclease proteins (enzymes), novel nuclease compositions and protein preparations thereof, recombinant polynucleotides (DNA) encoding such nuclease proteins, recombinant host cells expressing and producing one or more nuclease proteins (optionally co-expressing one or more proteins of interest) and the like. More particularly, the nuclease proteins (enzymes) of the instant disclosure are particularly useful in mitigating DNA contamination, such as contaminating DNA present in a fermentation broth in which microbial host cells have been fermented, contaminating DNA present in recovered proteins of interest, contaminating DNA present in protein preparations, and the like.
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公开(公告)号：US20220389474A1
公开(公告)日：2022-12-08
申请号：US17770269
申请日：2020-10-14
申请人： DANISCO US INC.
发明人： Sharief Barends , Cristina Bongiorni , Feng Guo , Lori Ann Maggio-Hall , Lawrence Philip Wackett , Gregory M. Whited
摘要： The present disclosure is generally related to the fields of biology, molecular biology, genetics, microbial host cells, industrial enzyme production, and the like. More particularly, certain embodiments of the disclosure are related to microbial host cells for the production of heterologous proteins, which microbial host cells are well-suited for growth in submerged cultures for the large-scale production of heterologous cyanuric acid hydrolases and biuret hydrolases.
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公开(公告)号：US10683528B2
公开(公告)日：2020-06-16
申请号：US15529948
申请日：2015-12-03
申请人： DANISCO US INC.
发明人： Cristina Bongiorni , Rodante Gonzales Caguiat , Carol Marie Fioresi , Brian F. Schmidt , Anita Van Kimmenade , Chao Zhu
摘要： The present invention relates in general to bacterial cells having genetic alterations that result in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include altered Gram positive microorganisms having one or more a genetic alterations that reduce the expression of a gene in the sin operon, thereby resulting in the enhanced expression of one or more proteins of interest.
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公开(公告)号：US11781147B2
公开(公告)日：2023-10-10
申请号：US17293644
申请日：2019-11-25
申请人： DANISCO US INC
摘要： The present disclosure is generally related to novel promoter sequences and methods thereof for enhanced protein production in Bacillus sp. (host) cells. As set forth herein, the novel promoter sequences of the disclosure, when operably linked to a gene or open reading frame encoding a protein of interest, are particularly well suited for use in large scale production of industrially relevant proteins.
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公开(公告)号：US10287591B2
公开(公告)日：2019-05-14
申请号：US15108074
申请日：2014-12-08
申请人： DANISCO US INC.
摘要： The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Gram positive microorganisms, such as Bacillus species, having a genetic alteration that modifies activity of a protein encoded by the ykf operon and results in enhanced expression of a protein of interest.
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公开(公告)号：US20180327757A1
公开(公告)日：2018-11-15
申请号：US15771618
申请日：2016-10-27
申请人： DANISCO US INC
CPC分类号： C12N15/75 , C12N9/1247 , C12N2320/50 , C12N2330/51 , C12N2800/101 , C12P21/00 , C12P21/02 , C12R1/01
摘要： The present disclosure is generally related to modified Gram positive bacterial cells producing increased amounts of one or more protein(s) of interest and modified Gram positive bacterial cells having increased genetic competency. Thus, certain embodiments of the disclosure are directed to modified Gram positive bacterial cells expressing an increased amount of a protein of interest, relative to an unmodified (parental) Gram positive bacterial cell expressing the same protein of interest, wherein the modified bacterial cell comprises at least one mutation in a rpoC gene encoding a variant RNA-polymerase (RNAP) β′-subunit polypeptide. In certain embodiments, the rpoC gene encoding the variant β′-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpoC gene encoding the variant β′-subunit polypeptide is comprised on an extrachromosomal plasmid introduced into the modified cell. In other embodiments, the disclosure is directed to competent Bacillus host cells comprising at least one copy of a nucleic acid construct encoding a modified rpoC polypeptide comprising 90% sequence identity to SEQ ID NO: 8 and an aspartic acid to glycine substitution at position 796 of SEQ ID NO: 8, wherein the polynucleotide encoding the rpoC polypeptide is foreign to the Bacillus host cell that was non-competent prior to the introduction of the first nucleic acid construct.
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公开(公告)号：US12077762B1
公开(公告)日：2024-09-03
申请号：US17818258
申请日：2022-08-08
申请人： DANISCO US INC.
发明人： Cristina Bongiorni , Marc Anton Bernhard Kolkman , Chris Leeflang , Virgil Arthur Rhodius , Anita Van Kimmenade
CPC分类号： C12N15/75 , C07K14/32 , C12N9/2417 , C12N9/54 , C12Y302/01001 , C12Y304/21062
摘要： The instant disclosure is generally related to novel engineered (hybrid) promoters. In certain embodiments, the instant disclosure is directed to one or more nucleic acid compositions comprising engineered promoters operably linked to nucleic acids encoding proteins of interest. Thus, the disclosure set forth herein described methods and compositions for the production of proteins of interest using one or more novel engineered (hybrid) promoters of the disclosure.
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公开(公告)号：US20140329309A1
公开(公告)日：2014-11-06
申请号：US14364016
申请日：2012-12-06
申请人： Danisco US Inc.
IPC分类号： C12N9/54
CPC分类号： C12N9/54 , C12N15/75 , C12Y304/21062
摘要： The present invention provides methods and compositions of improved expression systems in microorganisms. The methods and compositions comprise a ribosomal promoter derived from a Bacillus species microorganism, such a ribosomal RNA or a ribosomal protein promoter.
摘要翻译： 本发明提供了微生物中改进的表达系统的方法和组合物。 所述方法和组合物包含衍生自芽孢杆菌属物种微生物的核糖体启动子，例如核糖体RNA或核糖体蛋白质启动子。
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公开(公告)号：US08476042B2
公开(公告)日：2013-07-02
申请号：US13622968
申请日：2012-09-19
申请人： Danisco US Inc.
CPC分类号： C12N9/16 , C07K14/32 , C12N9/1205
摘要： The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified Bacillus sp. Host cells that have at least one inactivated phr gene. The enhanced production of proteins of interest by the modified Bacillus sp. Host cells is further increased in modified Bacillus sp. Host cells that overexpress YmaH. Methods for producing proteins of interest in the modified host cells are also provided.
摘要翻译： 本发明提供已被遗传操作以具有增强的产生感兴趣蛋白质的能力的宿主细胞。 特别地，本发明涉及改良的芽孢杆菌属 具有至少一个失活的phr基因的宿主细胞。 通过改良的芽孢杆菌菌种增强生产的目的蛋白质 宿主细胞在改良的芽孢杆菌中进一步增加。 宿主细胞过度表达YmaH。 还提供了在修饰的宿主细胞中产生目标蛋白质的方法。
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公开(公告)号：US20130029402A1
公开(公告)日：2013-01-31
申请号：US13622968
申请日：2012-09-19
申请人： Danisco US Inc.
CPC分类号： C12N9/16 , C07K14/32 , C12N9/1205
摘要： The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified Bacillus sp. Host cells that have at least one inactivated phr gene. The enhanced production of proteins of interest by the modified Bacillus sp. Host cells is further increased in modified Bacillus sp. Host cells that overexpress YmaH. Methods for producing proteins of interest in the modified host cells are also provided
摘要翻译： 本发明提供已被遗传操作以具有增强的产生感兴趣蛋白质的能力的宿主细胞。 特别地，本发明涉及改良的芽孢杆菌属 具有至少一个失活的phr基因的宿主细胞。 通过改良的芽孢杆菌菌种增强生产的目的蛋白质 宿主细胞在改良的芽孢杆菌中进一步增加。 宿主细胞过度表达YmaH。 还提供了在修饰的宿主细胞中产生目标蛋白质的方法
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