-
公开(公告)号：US20060014200A1
公开(公告)日：2006-01-19
申请号：US11225247
申请日：2005-09-12
申请人： William McMillan , Lee Christel , David Borkholder , Steven Young
发明人： William McMillan , Lee Christel , David Borkholder , Steven Young
CPC分类号： C12Q1/6851 , B01L7/52 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/645 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6471 , G01N2035/00366 , G01N2035/00376 , G01N2035/0097 , G01N2201/062 , G01N2201/0627 , C12Q2537/165 , C12Q2545/107 , C12Q2545/113 , C12Q2545/101
摘要： An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of a nucleic acid sequence being amplified in the reaction. A controller in communication with the detection mechanism is programmed to store signal values defining a growth curve for the nucleic acid sequence, determine a derivative of the growth curve, and calculate a cycle number or time value associated with a characteristic of the derivative.
-
2.发明申请公开(公告)号：US20050255516A1
公开(公告)日：2005-11-17
申请号：US11170620
申请日：2005-06-28
申请人： William McMillan , Lee Christel , David Borkholder , Steven Young
发明人： William McMillan , Lee Christel , David Borkholder , Steven Young
IPC分类号： B01L7/00 , C07H21/02 , C07H21/04 , C12M1/00 , C12M1/34 , C12N9/98 , C12P19/34 , C12Q1/00 , C12Q1/68 , G01N21/27 , G01N21/64 , G01N33/48 , G01N33/50 , G01N35/00 , G06F19/00
CPC分类号： C12Q1/6851 , B01L7/52 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/645 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6471 , G01N2035/00366 , G01N2035/00376 , G01N2035/0097 , G01N2201/062 , G01N2201/0627 , C12Q2537/165 , C12Q2545/107 , C12Q2545/113 , C12Q2545/101
摘要： A method for determining an unknown starting quantity of a target nucleic acid sequence in a test sample comprises the steps of amplifying the unknown starting quantity of the target nucleic acid sequence in the test sample and known starting quantities of a calibration nucleic acid sequence in respective calibration samples; and determining a respective threshold value for each of the nucleic acid sequences using a derivative of a growth curve derived for the sequence. The starting quantity of the target nucleic acid sequence in the test sample is determined using the threshold value determined for the target sequence and a calibration curve derived from the threshold values determined for the known starting quantities of the calibration nucleic acid sequences. The invention also provides methods for determining a starting quantity of a nucleic acid sequence in a sample using quantitative internal controls or using internal standards.
摘要翻译： 用于确定测试样品中目标核酸序列的未知起始量的方法包括以下步骤：扩增测试样品中目标核酸序列的未知起始量和已校准核酸序列的开始量在相应的校准 样品; 以及使用由该序列导出的生长曲线的衍生物来确定每个核酸序列的相应阈值。 使用针对靶序列确定的阈值和从针对校准核酸序列的已知起始量确定的阈值得到的校准曲线来确定测试样品中靶核酸序列的起始量。 本发明还提供了使用定量内部对照或使用内部标准确定样品中核酸序列的起始量的方法。
-
公开(公告)号：US20190322111A1
公开(公告)日：2019-10-24
申请号：US16388459
申请日：2019-04-18
申请人： Chaitanya G. Mahajan , Denis Cormier , Mark Irving , Scott Williams , David Borkholder , Ahmed Alfadhel
发明人： Chaitanya G. Mahajan , Denis Cormier , Mark Irving , Scott Williams , David Borkholder , Ahmed Alfadhel
摘要： An approach to printing a nickel precursor ink on a wide range of substrates for electronics and magnetic applications is disclosed. The nickel ink reduces to elemental nickel following heating. The ink was printed using an ultrasonic aerosol printing technique. By sintering the nickel precursor ink in the presence of a homogeneous magnetic field, the reduced nickel complex formed continuously aligned nickel nanofibers axially aligned with the direction of the magnetic field. The fabrication of aligned interlayered nanofiber films provides opportunities to produce structures with enhanced isotropic electrical and magnetic properties. The resistivity of the film was found to be as low as 0.56 mΩ·cm, and the saturation magnetization was measured to be 30 emu/g, which is comparable to bulk Ni. Magnetic anisotropy was induced with an easy axis along the direction of the applied magnetic field with soft magnetic properties.
-
公开(公告)号：US11254156B2
公开(公告)日：2022-02-22
申请号：US16388459
申请日：2019-04-18
申请人： Chaitanya G. Mahajan , Denis Cormier , Mark Irving , Scott Williams , David Borkholder , Ahmed Alfadhel
发明人： Chaitanya G. Mahajan , Denis Cormier , Mark Irving , Scott Williams , David Borkholder , Ahmed Alfadhel
摘要： An approach to printing a nickel precursor ink on a wide range of substrates for electronics and magnetic applications is disclosed. The nickel ink reduces to elemental nickel following heating. The ink was printed using an ultrasonic aerosol printing technique. By sintering the nickel precursor ink in the presence of a homogeneous magnetic field, the reduced nickel complex formed continuously aligned nickel nanofibers axially aligned with the direction of the magnetic field. The fabrication of aligned interlayered nanofiber films provides opportunities to produce structures with enhanced isotropic electrical and magnetic properties. The resistivity of the film was found to be as low as 0.56 mΩ·cm, and the saturation magnetization was measured to be 30 emu/g, which is comparable to bulk Ni. Magnetic anisotropy was induced with an easy axis along the direction of the applied magnetic field with soft magnetic properties.
-
5.发明授权公开(公告)号：US06377057B1
公开(公告)日：2002-04-23
申请号：US09251027
申请日：1999-02-18
申请人： David Borkholder
发明人： David Borkholder
IPC分类号： F02P1700
CPC分类号： G01N33/4836
摘要： Biologically active compounds are classified according to their effect on ion channels, changes in membrane potential and ionic currents, and the frequency content of action potentials that the compound(s) evoke in excitable cells. The spectral density changes of such evoked membrane potential or action potential are a characteristic for each channel type that is modulated by the test compound. A pattern of spectral changes in membrane potential is determined by contacting a responsive cell with a compound, and monitoring the membrane potential or ionic currents over time. These changes correlate with the effect of that compound, or class of compounds, on the ion channels of the responding cell. This pattern of spectral changes provides a unique signature for the compound, and provides a useful method for characterization of channel modulating agents.
摘要翻译： 生物活性化合物根据其对离子通道的影响，膜电位和离子电流的变化以及化合物在可兴奋细胞中诱发的动作电位的频率含量进行分类。 这种诱发膜电位或动作电位的光谱密度变化是由测试化合物调制的每种通道类型的特征。 通过使响应细胞与化合物接触并监测膜电位或离子电流随时间而确定膜电位的光谱变化的模式。 这些变化与该化合物或一类化合物对响应细胞的离子通道的影响相关。 这种光谱变化模式为化合物提供了独一无二的特征，并提供了一种用于表征通道调节剂的有用方法。
-
-
-
-
搜索结果
5 条记录 (耗时 0.012 秒)