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    RECOMBINANT BACTERIOPHAGE AND METHODS FOR THEIR USE
    2.
    发明申请
    RECOMBINANT BACTERIOPHAGE AND METHODS FOR THEIR USE 有权
    重组细菌及其使用方法

    公开(公告)号：US20110136688A1

    公开(公告)日：2011-06-09

    申请号：US12941717

    申请日：2010-11-08

    申请人： Dean SCHOLL ,  Steven Williams

    发明人： Dean SCHOLL ,  Steven Williams

    IPC分类号： C40B30/04 ,  C12N7/01 ,  C40B40/02 ,  C12Q1/70 ,  C07H21/00

    CPC分类号： C12N7/00 ,  C07K14/21 ,  C07K2319/035 ,  C07K2319/35 ,  C07K2319/74 ,  C12N2795/10143 ,  C12N2810/6009

    摘要： Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed as are methods for producing them in GRAS organisms. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.

    摘要翻译： 公开了天然存在的细菌素的修饰形式，例如绿脓假单胞菌的R型py霉素，作为在GRAS生物体中生产它们的方法。 细菌素在其尾纤维的末端被修饰，负责对其同源结合配偶体或受体（例如细菌表面上的那些）的结合特异性和亲和力。 还描述了在细菌表面上使用修饰的细菌素，例如结合受体（包括毒力或适应因子）的方法。

    Method for determining sensitivity to a bacteriophage
    4.
    发明申请
    Method for determining sensitivity to a bacteriophage 审中-公开
    确定对噬菌体敏感性的方法

    公开(公告)号：US20050118567A1

    公开(公告)日：2005-06-02

    申请号：US10498428

    申请日：2003-01-23

    申请人： Carl Merril ,  Sankar Adhya ,  Dean Scholl

    发明人： Carl Merril ,  Sankar Adhya ,  Dean Scholl

    IPC分类号： A01N63/00 ,  A61K20060101 ,  A61K35/76 ,  A61K39/00 ,  A61K48/00 ,  A61P31/04 ,  C12M1/34 ,  C12N7/01 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/569 ,  G01N33/68

    CPC分类号： G01N33/56911 ,  A61K35/76 ,  A61K2039/5256 ,  C12N2710/00032 ,  C12Q1/6897 ,  G01N33/6845 ,  Y02A50/478

    摘要： This disclosure provides methods of selecting a therapeutic bacteriophage and identifying bacteria in a sample. The sample may be obtained from a plant or animal subject diagnosed with a disease caused by a bacterial infection or an object suspected of being exposed to a bacterium. The activity of reporter molecules, either encoded in the bacteriophage genome or added during sample analysis, is used to determine whether bacteriophages are capable of infecting assayed bacteria. Also provided are methods of selecting a bacteriophage for potential use in treating of bacterial infection, based upon the selectivity of the bacteriophage host range for the bacterium. The bacteriophages or bacteria may be immobilized in an array, such that multiple bacteriophages and/or bacteria may be assayed. Kits are also provided.

    摘要翻译： 本公开提供了选择治疗性噬菌体并鉴定样品中的细菌的方法。 样品可以从被诊断患有由细菌感染引起的疾病或疑似暴露于细菌的物体的植物或动物受试者获得。 在噬菌体基因组中编码或在样品分析过程中加入的报道分子的活性用于确定噬菌体是否能够感染测定的细菌。 还提供了基于细菌的噬菌体宿主范围的选择性来选择用于治疗细菌感染的潜在用途的噬菌体的方法。 噬菌体或细菌可以固定在阵列中，使得可以测定多种噬菌体和/或细菌。 还提供套件。

    Reporter plasmid phage packaging system for detection of bacteria
    5.
    发明申请
    Reporter plasmid phage packaging system for detection of bacteria 审中-公开
    报告质粒噬菌体包装系统用于检测细菌

    公开(公告)号：US20090155768A1

    公开(公告)日：2009-06-18

    申请号：US11547587

    申请日：2005-04-07

    申请人： Dean Scholl ,  Carl R. Merril ,  Sankar Adhya ,  Michael McKinstry ,  Rotem Edgar

    发明人： Dean Scholl ,  Carl R. Merril ,  Sankar Adhya ,  Michael McKinstry ,  Rotem Edgar

    IPC分类号： C12Q1/70 ,  C12N15/00 ,  C12N15/87

    CPC分类号： C12Q1/70 ,  C12N15/70 ,  C12Q1/6897

    摘要： The invention is related to a transducing particle that comprises a bacteriophage coat and a DNA core that comprises plasmid DNA comprising: a) a host-specific bacteriophage packaging site wherein the packaging site is substantially in isolation from sequences naturally occurring adjacent thereto in the bacteriophage genome, b) a reporter gene, c) a bacteria-specific promoter operably linked to said reporter gene, d) a bacteria-specific origin of replication, and optionally e) an antibiotic resistance gene. The invention includes phage transducing particles, methods of making transducing particles, and methods of using the transducing particles in bacterial detection.

    摘要翻译： 本发明涉及包含噬菌体外壳和包含质粒DNA的DNA核心的转导颗粒，所述质粒DNA包含：a）宿主特异性噬菌体包装位点，其中所述包装位点基本上与噬菌体基因组中天然存在的序列分离 b）报告基因，c）可操作地连接到所述报告基因的细菌特异性启动子，d）细菌特异性复制起点，以及e）抗生素抗性基因。 本发明包括噬菌体转导颗粒，制备转导颗粒的方法，以及在细菌检测中使用转导颗粒的方法。