公开(公告)号：US20140271691A1

公开(公告)日：2014-09-18

申请号：US14073210

申请日：2013-11-06

申请人： Biswajit Biswas ,  Carl R. Merril ,  Hossein A. Ghanbari

发明人： Biswajit Biswas ,  Carl R. Merril ,  Hossein A. Ghanbari

IPC分类号： A61K39/00

CPC分类号： A61K39/0011 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/6075 ,  C07K14/4748 ,  C07K2319/735 ,  C12N9/0071 ,  C12N2795/10342 ,  C12N2795/10343 ,  C12Y114/11016

摘要： The present invention encompasses a cancer vaccine therapy targeting Aspartyl-[Asparaginyl]-β-hydroxylase (HAAH). The present invention contemplate bacteriophage expressing HAAH peptide fragments and methods for using said bacteriophage in methods of treating cancer.

摘要翻译： 本发明包括靶向天冬氨酰 - [Asparaginyl] - ＆bgr。 - 羟化酶（HAAH）的癌症疫苗疗法。 本发明考虑了表达HAAH肽片段的噬菌体和在治疗癌症的方法中使用所述噬菌体的方法。

公开(公告)号：US5861504A

公开(公告)日：1999-01-19

申请号：US952277

申请日：1992-09-28

申请人： Mihael H. Polymeropoulos ,  Carl R. Merril

发明人： Mihael H. Polymeropoulos ,  Carl R. Merril

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6876 ,  C12Q1/6858 ,  C12Q2600/156

摘要： The invention relates to polymorphic markers (two tetranucleotide, one dinucleotide repeat polymorphisms, 27 markers characterized by primer pairs 1A-27A, and eleven markers characterized by primer pairs 1B-11B that are useful for human individualization. Applications are in forensic medicine and for paternity and prenatal screening as well as genetic mapping. These markers are characterized by sets of oligonucleotide primers according to the invention useful in PCR amplification and DNA segment resolution. The invention further relates to an assay for measuring the subtle differences in genetic material regarding an added or omitted set of dinucleotide or tetranucleotide repeat polymorphisms which comprises obtaining an amount of nucleotide segments effective for testing, amplifying the segments by the PCR procedure using at least one primer nucleotide sequence according to the present invention, resolving the amplified segments using gel electrophoresis, and comparing the resolved segments by autoradiography to observe the differences in migration patterns due to structural differences. The assay according to the invention is easy to perform and results can be obtained within 24 hours. It is not uncommon for results to be available within 3-4 hours. Accordingly, the invention also relates to an improved PCR procedure and a PCR assay kit which comprise nucleotides according to the invention.

摘要翻译： 本发明涉及多态性标记（两个四核苷酸，一个二核苷酸重复多态性，27个引物对1A-27A特征的标记，以及引物对1B-11B特征的11个标记，可用于人的个体化，应用于法医学和亲子鉴定 本发明还涉及用于测量遗传物质中关于添加或添加的遗传物质的微妙差异的测定法， 省略了一组二核苷酸或四核苷酸重复多态性，其包括获得有效用于测试的量的核苷酸区段，使用至少一个本发明引物核苷酸序列通过PCR程序扩增片段，使用凝胶电泳解析扩增片段，并比较 解决了 通过放射自显影来观察由于结构差异导致的迁移模式的差异。 根据本发明的测定容易进行，并且可以在24小时内获得结果。 结果在3-4小时内可以使用并不罕见。 因此，本发明还涉及包含根据本发明的核苷酸的改进的PCR方法和PCR测定试剂盒。

公开(公告)号：US5817797A

公开(公告)日：1998-10-06

申请号：US200876

申请日：1988-06-01

申请人： Lloyd G. Mitchell ,  Carl R. Merril

发明人： Lloyd G. Mitchell ,  Carl R. Merril

IPC分类号： C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6844 ,  C07H21/00 ,  C12N15/1096 ,  C12Q1/6869

摘要： The invention provides a process wherein a biotinylated oligonucleotide primer and an oligonucleotide primer which has not undergone biotinylation are used when amplifying a DNA sequence to facilitate separation of the DNA strands following the polymerase chain reaction process. The biotinylation/PCR product is then exposed to a support which will selectively bind the biotinylated strand to allow selective elution of the product.

摘要翻译： 本发明提供一种方法，其中当扩增DNA序列以促进聚合酶链式反应方法后的DNA链分离时，使用未经历生物素化的生物素化寡核苷酸引物和寡核苷酸引物。 然后将生物素化/ PCR产物暴露于支持物，其将选择性结合生物素化的链，以允许产物的选择性洗脱。

公开(公告)号：US5468610A

公开(公告)日：1995-11-21

申请号：US74275

申请日：1993-06-09

申请人： Mihael H. Polymeropoulos ,  Carl R. Merril

发明人： Mihael H. Polymeropoulos ,  Carl R. Merril

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q1/6876 ,  C12Q2600/156

摘要： The invention relates to polymorphic markers (two tetranucleotide and one dinucleotide repeat polymorphisms) that are useful for human individualization. Applications are in forensic medicine and for paternity and prenatal screening as well as genetic mapping. These markers are characterized by sets of oligonucleotide primers according to the invention useful in PCR amplification and DNA segment resolution. The invention further relates to an assay for measuring the subtle differences in genetic material regarding an added or omitted set of dinucleotide or tetranucleotide repeat polymorphisms which comprises obtaining an amount of nucleotide segments effective for testing, amplifying the segments by the PCR procedure using at least one primer nucleotide sequence according to the present invention, resolving the amplified segments using gel electrophoresis, and comparing the resolved segments by autoradiography to observe the differences in migration patterns due to structural differences. The assay according to the invention is easy to perform and results can be obtained within 24 hours. It is not uncommon for results to be available within 3-4 hours. Accordingly, the invention also relates to an improved PCR procedure and a PCR assay kit which comprise nucleotides according to the invention.

摘要翻译： 本发明涉及可用于人类个体化的多态性标记（两个四核苷酸和一个二核苷酸重复多态性）。 应用于法医学和亲子鉴定和产前筛查以及基因测绘。 这些标记的特征在于本发明的可用于PCR扩增和DNA片段拆分的一组寡核苷酸引物。 本发明还涉及用于测量关于添加或省略的二核苷酸或四核苷酸重复多态性的集合的遗传物质的微妙差异的测定法，其包括获得有效测试的量的核苷酸区段，通过PCR程序使用至少一个 本发明的引物核苷酸序列，使用凝胶电泳解析扩增片段，并通过放射自显影比较分辨的片段以观察由于结构差异引起的迁移模式的差异。 根据本发明的测定容易进行，并且可以在24小时内获得结果。 结果在3-4小时内可以使用并不罕见。 因此，本发明还涉及包含根据本发明的核苷酸的改进的PCR方法和PCR测定试剂盒。

公开(公告)号：US5364793A

公开(公告)日：1994-11-15

申请号：US983443

申请日：1992-12-01

申请人： Bruce M. Cameron, Sr. ,  Carl R. Merril ,  Guy J. Creed ,  Dale VanderPutten

发明人： Bruce M. Cameron, Sr. ,  Carl R. Merril ,  Guy J. Creed ,  Dale VanderPutten

IPC分类号： A61K39/395 ,  C07K16/18 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N27/447 ,  G01N33/50 ,  G01N33/53 ,  G01N33/561 ,  G01N33/577 ,  G01N33/68 ,  G01N33/49 ,  C12Q1/00 ,  G01N27/26 ,  G01N33/487

CPC分类号： G01N33/6896 ,  C07K16/18 ,  G01N33/561 ,  G01N33/68 ,  G01N33/6893 ,  G01N2800/10 ,  G01N2800/2842

摘要： Methods of diagnosing peripheral nerve damage, including diagnosing and monitoring chronic back and cervical pain are disclosed. The methods involve subjecting a body fluid sample from a patient suspected of having chronic lumbar or cervical pain and peripheral nerve damage to two-dimensional electrophoresis or an immunoassay and measuring relative amounts of protein or proteins which increase or decrease in concentration as compared to a standard control. A preferred method employs an Apo-E variant as a marker of peripheral nerve damage. Also disclosed are kits for use with the diagnostic methods.

摘要翻译： 公开了诊断周围神经损伤的方法，包括诊断和监测慢性背部和颈部疼痛。 所述方法包括将疑似患有慢性腰椎或颈部疼痛和周围神经损伤的患者的体液样品进行二维电泳或免疫测定，并测量与标准物相比增加或减少浓度的蛋白质或蛋白质的相对量 控制。 优选的方法是使用Apo-E变体作为周围神经损伤的标记物。 还公开了用于诊断方法的试剂盒。

公开(公告)号：US4508820A

公开(公告)日：1985-04-02

申请号：US582862

申请日：1984-02-27

申请人： Carl R. Merril ,  David Goldman

发明人： Carl R. Merril ,  David Goldman

IPC分类号： G01N33/60 ,  G01N33/68 ,  C12Q1/16 ,  G01T1/00

CPC分类号： G01N33/60 ,  G01N33/6836

摘要： The invention provides an assay method for cellular and extracellular protein biodynamics comprising the use of radiochemical techniques in combination with high resolution protein separation procedures and recently developed silver stain techniques, to assess turnover of polypeptides over time. Quenching of polypeptide radiolables in silver stained gels is obviated by the use of .sup.14 C-labelled amino acids as precursors.

摘要翻译： 本发明提供了细胞和细胞外蛋白质生物动力学的测定方法，其包括使用放射化学技术结合高分辨率蛋白质分离方法和最近开发的银染技术，以评估多肽随时间变化。 通过使用14C标记的氨基酸作为前体，消除了银染色凝胶中的多肽可放射性的猝灭。

公开(公告)号：US20090203143A1

公开(公告)日：2009-08-13

申请号：US12135247

申请日：2008-06-09

申请人： Lloyd G. Mitchell ,  Edward Otto ,  Carl R. Merril

发明人： Lloyd G. Mitchell ,  Edward Otto ,  Carl R. Merril

IPC分类号： C12N15/87 ,  C12N15/11 ,  C12N1/21

CPC分类号： C12N15/10 ,  C12N15/1027 ,  C12N15/1086 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/3519 ,  C12N2320/33 ,  C12N2840/445

摘要： The present invention provides methods and compositions for conferring selective death on cells expressing a specific target precursor messenger RNA (selective target pre-mRNA). The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) expressed within a cell and mediate a trans-splicing reaction resulting in the generation of a novel chimeric mRNA molecule (chimeric mRNA) capable of encoding a light producing protein or enzyme. Cell death is further mediated by the presence of a photosensitizer which upon photoactivation produces cytotoxicity.

摘要翻译： 本发明提供了赋予表达特异性靶标前体信使RNA（选择性靶前体mRNA）的细胞上选择性死亡的方法和组合物。 本发明的组合物包括设计成与在细胞内表达的靶前体信使RNA分子（靶前体mRNA）相互作用的预转录分子（PTM），并介导反剪接反应，导致产生新的嵌合 能够编码产生光的蛋白质或酶的mRNA分子（嵌合mRNA）。 细胞死亡进一步由光敏剂的存在介导，光敏剂在光活化时产生细胞毒性。

公开(公告)号：US20090155768A1

公开(公告)日：2009-06-18

申请号：US11547587

申请日：2005-04-07

申请人： Dean Scholl ,  Carl R. Merril ,  Sankar Adhya ,  Michael McKinstry ,  Rotem Edgar

发明人： Dean Scholl ,  Carl R. Merril ,  Sankar Adhya ,  Michael McKinstry ,  Rotem Edgar

IPC分类号： C12Q1/70 ,  C12N15/00 ,  C12N15/87

CPC分类号： C12Q1/70 ,  C12N15/70 ,  C12Q1/6897

摘要： The invention is related to a transducing particle that comprises a bacteriophage coat and a DNA core that comprises plasmid DNA comprising: a) a host-specific bacteriophage packaging site wherein the packaging site is substantially in isolation from sequences naturally occurring adjacent thereto in the bacteriophage genome, b) a reporter gene, c) a bacteria-specific promoter operably linked to said reporter gene, d) a bacteria-specific origin of replication, and optionally e) an antibiotic resistance gene. The invention includes phage transducing particles, methods of making transducing particles, and methods of using the transducing particles in bacterial detection.

摘要翻译： 本发明涉及包含噬菌体外壳和包含质粒DNA的DNA核心的转导颗粒，所述质粒DNA包含：a）宿主特异性噬菌体包装位点，其中所述包装位点基本上与噬菌体基因组中天然存在的序列分离 b）报告基因，c）可操作地连接到所述报告基因的细菌特异性启动子，d）细菌特异性复制起点，以及e）抗生素抗性基因。 本发明包括噬菌体转导颗粒，制备转导颗粒的方法，以及在细菌检测中使用转导颗粒的方法。

公开(公告)号：US07521479B2

公开(公告)日：2009-04-21

申请号：US09835537

申请日：2001-04-16

申请人： Carl R. Merril ,  Hossein A. Ghanbari

发明人： Carl R. Merril ,  Hossein A. Ghanbari

IPC分类号： A01N37/52 ,  A61K31/155

CPC分类号： A61K31/17 ,  A61K31/155 ,  A61K33/18

摘要： The invention is related to the treatment of prion-related diseases such as the transmissible spongiform encephalopathies (TSEs) in mammals by administering chaotropic agents to or inducing a hyperthermia state in the affected mammals.

公开(公告)号：US07399753B2

公开(公告)日：2008-07-15

申请号：US10658617

申请日：2003-09-09

申请人： Lloyd G. Mitchell ,  Edward Otto ,  Carl R. Merril

发明人： Lloyd G. Mitchell ,  Edward Otto ,  Carl R. Merril

IPC分类号： C12N5/10 ,  C12N15/87 ,  C12N15/63 ,  C12N15/66 ,  C12N15/00 ,  A61K31/711 ,  C12N15/861

CPC分类号： C12N15/10 ,  C12N15/1027 ,  C12N15/1086 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/3519 ,  C12N2320/33 ,  C12N2840/445

摘要： The present invention provides methods and compositions for conferring selective death on cells expressing a specific target precursor messenger RNA (selective target pre-mRNA). The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) expressed within a cell and mediate a trans-splicing reaction resulting in the generation of a novel chimeric mRNA molecule (chimeric mRNA) capable of encoding a light producing protein or enzyme. Cell death is further mediated by the presence of a photosensitizer which upon photoactivation produces cytotoxicity.

摘要翻译： 本发明提供了赋予表达特异性靶标前体信使RNA（选择性靶前体mRNA）的细胞上选择性死亡的方法和组合物。 本发明的组合物包括设计成与在细胞内表达的靶前体信使RNA分子（靶前体mRNA）相互作用的预转录分子（PTM），并介导反剪接反应，导致产生新的嵌合 能够编码产生光的蛋白质或酶的mRNA分子（嵌合mRNA）。 细胞死亡进一步由光敏剂的存在介导，光敏剂在光活化时产生细胞毒性。