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10 条记录 (耗时 0.018 秒)

    HIGH-RESOLUTION MELTING ANALYSIS
    6.
    发明申请
    HIGH-RESOLUTION MELTING ANALYSIS 有权
    高分辨率熔融分析

    公开(公告)号：US20120239305A1

    公开(公告)日：2012-09-20

    申请号：US13429911

    申请日：2012-03-26

    申请人： Deborah BOLES ,  Kenton C. Hasson ,  Sami Kanderian

    发明人： Deborah BOLES ,  Kenton C. Hasson ,  Sami Kanderian

    IPC分类号： G06F19/10

    CPC分类号： G06F19/24 ,  B01L7/52 ,  C12Q1/6816 ,  G06F19/18 ,  G06F19/20 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

    摘要： The application relates to methods and systems for analysis of dissociation behavior of nucleic acids and identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. Methods and systems are also disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

    摘要翻译： 该申请涉及用于分析核酸的解离行为和核酸鉴定的方法和系统。 在一个方面，公开了用于鉴定样品中的核酸的方法和系统，所述样品包括未知核酸并用于检测样品中核酸中的单核苷酸多态性。 还公开了用于通过将包含多个独立样品特性点的样品的可量化物理变化的测量的变性数据拟合到确定的多个独立样品特性点的功能来鉴定包含至少一种未知核酸的生物样品中的核酸的方法和系统 内在物理价值，并获得估计的物理变化功能，以及通过将至少一种未知核酸的固有物理值与已知核酸的固有物理值进行比较来鉴定生物样品中的核酸。

    HIGH-RESOLUTION MELTING ANALYSIS
    7.
    发明申请
    HIGH-RESOLUTION MELTING ANALYSIS 有权
    高分辨率熔融分析

    公开(公告)号：US20090112484A1

    公开(公告)日：2009-04-30

    申请号：US12258098

    申请日：2008-10-24

    申请人： Deborah BOLES ,  Kenton C. HASSON ,  Sami KANDERIAN

    发明人： Deborah BOLES ,  Kenton C. HASSON ,  Sami KANDERIAN

    IPC分类号： G06F19/00

    CPC分类号： G06F19/24 ,  B01L7/52 ,  C12Q1/6816 ,  G06F19/18 ,  G06F19/20 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

    摘要： The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. In another aspect, methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

    摘要翻译： 本发明涉及用于分析核酸的解离行为和核酸鉴定的方法和系统。 在一个方面，公开了用于鉴定样品中的核酸的方法和系统，所述样品包括未知核酸并用于检测样品中核酸中的单核苷酸多态性。 在另一方面，公开了用于通过拟合变性数据来包括至少一种未知核酸的生物样品中的核酸的鉴定的方法和系统，所述变性数据包括在多个独立样本特性点处的样品的可量化物理变化的测量值 用于确定固有物理值并获得估计的物理变化功能，以及通过将至少一种未知核酸的固有物理值与已知核酸的固有物理值进行比较来鉴定生物样品中的核酸。

    Systems for and methods of hybrid pyrosequencing
    8.
    发明授权
    Systems for and methods of hybrid pyrosequencing 有权
    混合焦磷酸测序系统和方法

    公开(公告)号：US08901043B2

    公开(公告)日：2014-12-02

    申请号：US13542721

    申请日：2012-07-06

    申请人： Allen E. Eckhardt ,  Jonathan Benton ,  Deborah Boles

    发明人： Allen E. Eckhardt ,  Jonathan Benton ,  Deborah Boles

    IPC分类号： C40B30/00 ,  C12N15/10 ,  C12Q1/68 ,  G06F19/22 ,  G06F19/24

    CPC分类号： C12N15/1089 ,  C12Q1/6869 ,  C12Q1/689 ,  C12Q1/701 ,  C12Q1/703 ,  G06F19/22 ,  G06F19/24 ,  C12Q2537/165 ,  C12Q2565/301

    摘要： The systems and methods of the invention provide a guided approach to pyrosequencing (i.e., hybrid pyrosequencing). A de novo nucleic acid sequence may compared to a library of possible results and the next nucleotide to be dispensed is selected based on the comparison of the de novo sequence and the library of possible results. In another example, at least the first nucleotide to be dispensed is selected based on a query of a database(s) of non-sequence parameters (e.g., incidence of infection, diagnostic symptoms, sample source) and subsequent dispensations determined based on a comparison of the de novo sequence and the library of possible results (e.g., candidate sequences). The systems and methods of the invention may be performed using a droplet actuator.

    摘要翻译： 本发明的系统和方法提供了焦磷酸测序（即，杂化焦磷酸测序）的指导方法。 从头核酸序列可以与可能结果的文库进行比较，并且基于从头序列与可能结果库的比较来选择待分配的下一个核苷酸。 在另一个实例中，基于对非序列参数（例如，感染发生率，诊断症状，样本来源）的数据库的查询和基于比较确定的随后分配来选择至少要分配的第一核苷酸 的新生序列和可能结果的库（例如候选序列）。 本发明的系统和方法可以使用液滴致动器来执行。

    High-resolution melting analysis
    9.
    发明授权
    High-resolution melting analysis 有权
    高分辨率熔解分析

    公开(公告)号：US08606529B2

    公开(公告)日：2013-12-10

    申请号：US13429911

    申请日：2012-03-26

    申请人： Deborah Boles ,  Kenton C. Hasson ,  Sami Kanderian

    发明人： Deborah Boles ,  Kenton C. Hasson ,  Sami Kanderian

    IPC分类号： G06F19/00

    CPC分类号： G06F19/24 ,  B01L7/52 ,  C12Q1/6816 ,  G06F19/18 ,  G06F19/20 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

    摘要： The application relates to methods and systems for analysis of dissociation behavior of nucleic acids and identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. Methods and systems are also disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

    摘要翻译： 该申请涉及用于分析核酸的解离行为和核酸鉴定的方法和系统。 在一个方面，公开了用于鉴定样品中的核酸的方法和系统，所述样品包括未知核酸并用于检测样品中核酸中的单核苷酸多态性。 还公开了用于通过将包含多个独立样品特性点的样品的可量化物理变化的测量的变性数据拟合到确定的多个独立样品特性点的功能来鉴定包含至少一种未知核酸的生物样品中的核酸的方法和系统 内在物理价值，并获得估计的物理变化功能，以及通过将至少一种未知核酸的固有物理值与已知核酸的固有物理值进行比较来鉴定生物样品中的核酸。

    High-resolution melting analysis
    10.
    发明授权
    High-resolution melting analysis 有权
    高分辨率熔解分析

    公开(公告)号：US08145433B2

    公开(公告)日：2012-03-27

    申请号：US12258098

    申请日：2008-10-24

    申请人： Deborah Boles ,  Kenton C. Hasson ,  Sami Kanderian

    发明人： Deborah Boles ,  Kenton C. Hasson ,  Sami Kanderian

    IPC分类号： G06F19/00

    CPC分类号： G06F19/24 ,  B01L7/52 ,  C12Q1/6816 ,  G06F19/18 ,  G06F19/20 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

    摘要： The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. In another aspect, methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

    摘要翻译： 本发明涉及用于分析核酸的解离行为和核酸鉴定的方法和系统。 在一个方面，公开了用于鉴定样品中的核酸的方法和系统，所述样品包括未知核酸并用于检测样品中核酸中的单核苷酸多态性。 在另一方面，公开了用于通过拟合变性数据来包括至少一种未知核酸的生物样品中的核酸的鉴定的方法和系统，所述变性数据包括在多个独立样本特性点处的样品的可量化物理变化的测量值 用于确定固有物理值并获得估计的物理变化功能，以及通过将至少一种未知核酸的固有物理值与已知核酸的固有物理值进行比较来鉴定生物样品中的核酸。