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    High-resolution melting analysis
    1.
    发明授权
    High-resolution melting analysis 有权
    高分辨率熔解分析

    公开(公告)号：US08606529B2

    公开(公告)日：2013-12-10

    申请号：US13429911

    申请日：2012-03-26

    申请人： Deborah Boles ,  Kenton C. Hasson ,  Sami Kanderian

    发明人： Deborah Boles ,  Kenton C. Hasson ,  Sami Kanderian

    IPC分类号： G06F19/00

    CPC分类号： G06F19/24 ,  B01L7/52 ,  C12Q1/6816 ,  G06F19/18 ,  G06F19/20 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

    摘要： The application relates to methods and systems for analysis of dissociation behavior of nucleic acids and identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. Methods and systems are also disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

    摘要翻译： 该申请涉及用于分析核酸的解离行为和核酸鉴定的方法和系统。 在一个方面，公开了用于鉴定样品中的核酸的方法和系统，所述样品包括未知核酸并用于检测样品中核酸中的单核苷酸多态性。 还公开了用于通过将包含多个独立样品特性点的样品的可量化物理变化的测量的变性数据拟合到确定的多个独立样品特性点的功能来鉴定包含至少一种未知核酸的生物样品中的核酸的方法和系统 内在物理价值，并获得估计的物理变化功能，以及通过将至少一种未知核酸的固有物理值与已知核酸的固有物理值进行比较来鉴定生物样品中的核酸。

    High-resolution melting analysis
    2.
    发明授权
    High-resolution melting analysis 有权
    高分辨率熔解分析

    公开(公告)号：US08145433B2

    公开(公告)日：2012-03-27

    申请号：US12258098

    申请日：2008-10-24

    申请人： Deborah Boles ,  Kenton C. Hasson ,  Sami Kanderian

    发明人： Deborah Boles ,  Kenton C. Hasson ,  Sami Kanderian

    IPC分类号： G06F19/00

    CPC分类号： G06F19/24 ,  B01L7/52 ,  C12Q1/6816 ,  G06F19/18 ,  G06F19/20 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

    摘要： The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. In another aspect, methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

    摘要翻译： 本发明涉及用于分析核酸的解离行为和核酸鉴定的方法和系统。 在一个方面，公开了用于鉴定样品中的核酸的方法和系统，所述样品包括未知核酸并用于检测样品中核酸中的单核苷酸多态性。 在另一方面，公开了用于通过拟合变性数据来包括至少一种未知核酸的生物样品中的核酸的鉴定的方法和系统，所述变性数据包括在多个独立样本特性点处的样品的可量化物理变化的测量值 用于确定固有物理值并获得估计的物理变化功能，以及通过将至少一种未知核酸的固有物理值与已知核酸的固有物理值进行比较来鉴定生物样品中的核酸。

    HIGH-RESOLUTION MELTING ANALYSIS
    3.
    发明申请
    HIGH-RESOLUTION MELTING ANALYSIS 有权
    高分辨率熔融分析

    公开(公告)号：US20120239305A1

    公开(公告)日：2012-09-20

    申请号：US13429911

    申请日：2012-03-26

    申请人： Deborah BOLES ,  Kenton C. Hasson ,  Sami Kanderian

    发明人： Deborah BOLES ,  Kenton C. Hasson ,  Sami Kanderian

    IPC分类号： G06F19/10

    CPC分类号： G06F19/24 ,  B01L7/52 ,  C12Q1/6816 ,  G06F19/18 ,  G06F19/20 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

    摘要： The application relates to methods and systems for analysis of dissociation behavior of nucleic acids and identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. Methods and systems are also disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

    摘要翻译： 该申请涉及用于分析核酸的解离行为和核酸鉴定的方法和系统。 在一个方面，公开了用于鉴定样品中的核酸的方法和系统，所述样品包括未知核酸并用于检测样品中核酸中的单核苷酸多态性。 还公开了用于通过将包含多个独立样品特性点的样品的可量化物理变化的测量的变性数据拟合到确定的多个独立样品特性点的功能来鉴定包含至少一种未知核酸的生物样品中的核酸的方法和系统 内在物理价值，并获得估计的物理变化功能，以及通过将至少一种未知核酸的固有物理值与已知核酸的固有物理值进行比较来鉴定生物样品中的核酸。

    THERMAL CALIBRATION
    7.
    发明申请
    THERMAL CALIBRATION 审中-公开
    热校准

    公开(公告)号：US20120178077A1

    公开(公告)日：2012-07-12

    申请号：US13223258

    申请日：2011-08-31

    申请人： Fernando DeCastro ,  Renee Howell ,  Sami Kanderian ,  Johnathan S. Coursey ,  Kenton C. Hasson ,  Scott Sundberg

    发明人： Fernando DeCastro ,  Renee Howell ,  Sami Kanderian ,  Johnathan S. Coursey ,  Kenton C. Hasson ,  Scott Sundberg

    IPC分类号： G01N25/04 ,  C09K3/00 ,  G01K15/00 ,  C07H21/04

    CPC分类号： G01K11/06 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/0816 ,  B01L2300/1827 ,  C12Q1/6806 ,  G01K15/00 ,  C12Q2527/107 ,  C12Q2531/113 ,  C12Q2545/101 ,  C12Q2545/113 ,  C12Q2545/114 ,  C12Q2565/629

    摘要： The present invention relates to the use of one or more amplicons as temperature calibrators. In some embodiments, the calibrators may be used to calibrate the temperature of a microfluidic channel in which amplification and/or melt analysis is performed. In some embodiments, the amplicons may be genomic, ultra conserved elements and/or synthetic. The amplicon(s) may have a known or expected melt temperature(s). The calibrators may be added to primers of study or may follow or lead the primers of study in the channel. The amplicon(s) may be amplified and melted, and the temperature(s) at which the amplicon(s) melted may be determined. The measured temperature(s) may be compared to the known temperature(s) at which the amplicon(s) was expected to melt. The difference(s) between the measured and expected temperatures may be used to calibrate/adjust one or more temperature control elements used to control and/or detect the temperature of the channel.

    摘要翻译： 本发明涉及一种或多种扩增子作为温度校准剂的用途。 在一些实施方案中，校准物可用于校准其中进行扩增和/或熔融分析的微流体通道的温度。 在一些实施方案中，扩增子可以是基因组，超保守元件和/或合成的。 扩增子可以具有已知或预期的熔融温度。 校准物可以加入到研究的引物中，或者可以跟踪或引导通道中研究的引物。 可以扩增和融合扩增子，并且可以确定扩增子熔化的温度。 测量的温度可以与扩增子预期熔化的已知温度进行比较。 测量温度和预期温度之间的差异可用于校准/调节用于控制和/或检测通道温度的一个或多个温度控制元件。

    Rapid method of pattern recognition, machine learning, and automated genotype classification through correlation analysis of dynamic signals
    10.
    发明授权
    Rapid method of pattern recognition, machine learning, and automated genotype classification through correlation analysis of dynamic signals 有权
    通过动态信号的相关分析，模式识别，机器学习和自动化基因型分类的快速方法

    公开(公告)号：US08412466B2

    公开(公告)日：2013-04-02

    申请号：US12759415

    申请日：2010-04-13

    申请人： Sami Kanderian

    发明人： Sami Kanderian

    IPC分类号： G06F19/00 ,  G06F15/00 ,  C12Q1/68

    CPC分类号： G06F19/22 ,  C12Q1/6827 ,  G06F19/24 ,  C12Q2527/101 ,  C12Q2527/107 ,  C12Q2537/165

    摘要： The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of determining whether a genotype is present in a biological sample. This includes methods and systems for determining whether a genotype is present in a biological sample, through generating a dynamic profile an unknown genotype, correlating the dynamic profile to an average profile for a known genotype to generate a correlation value, and determining whether the correlation value falls within an acceptable threshold to determine if the unknown genotype is the known genotype. The present invention also relates to methods and systems for generating a training set to allow a machine to recognize a known genotype from within a class of known genotypes. The training set generated by these methods and systems may be used to assist in identification of unknown genotypes.

    摘要翻译： 本发明涉及用于分析核酸解离行为的方法和系统以及确定基因型是否存在于生物样品中的鉴定。 这包括用于通过生成未知基因型的动态分布来确定基因型是否存在于生物样品中的方法和系统，将动态分布与已知基因型的平均分布相关联以产生相关值，以及确定相关值 落在可接受的阈值内，以确定未知的基因型是否是已知的基因型。 本发明还涉及用于产生训练集以允许机器从一类已知基因型中识别已知基因型的方法和系统。 由这些方法和系统产生的训练集可用于帮助识别未知基因型。