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1.发明申请Method and apparatus for generating thermal melting curves in a microfluidic device 有权用于在微流体装置中产生热解曲线的方法和装置公开(公告)号：US20070026421A1
公开(公告)日：2007-02-01
申请号：US11352452
申请日：2006-02-09
申请人： Steven Sundberg , Michael Knapp , Ivor Knight , Deborah Boles , Aaron Rulison , Wesley Dong , Edward Donlon , Robert Moti , Andrew Fabans , Allen Boronkay , Michael Slater
发明人： Steven Sundberg , Michael Knapp , Ivor Knight , Deborah Boles , Aaron Rulison , Wesley Dong , Edward Donlon , Robert Moti , Andrew Fabans , Allen Boronkay , Michael Slater
IPC分类号： C12Q1/68
CPC分类号： G01N25/04 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/52 , B01L7/525 , B01L2200/10 , B01L2300/0627 , B01L2300/0816 , B01L2300/14 , B01L2300/18 , B01L2300/1833 , B01L2400/0409 , B01L2400/0415 , B01L2400/0487 , C12Q1/68 , C12Q1/6844 , C12Q1/686 , C12Q1/6862 , G01N21/33 , G01N21/6428 , Y10T436/143333 , C12Q2565/629 , C12Q2565/101 , C12Q2527/107 , C12Q2527/101
摘要： The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.
摘要翻译： 本发明提供了采用微流体技术产生分子熔融曲线的新颖方法和装置。 特别地，根据本发明的装置和方法可用于提供PCR扩增产物的分析。
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公开(公告)号：US07518726B2
公开(公告)日：2009-04-14
申请号：US11404043
申请日：2006-04-12
IPC分类号： G01B11/00
CPC分类号： G01B11/002 , B01L3/5027 , B01L3/502715 , B01L2200/025 , B01L2300/0816 , G01N21/645 , G01N2021/0162 , G01N2021/6419 , G01N2021/6421 , G01N2021/6478 , G01N2201/0627 , G02B21/16
摘要： An optical detection system for a microfluidic device and a dry-focus microfluidic device compatible with the compact optical detection system are described. The system includes an LED; means for collimating light emitted by the LED; an aspherical, fused-silica objective lens; means for directing the collimated light through the objective onto a microfluidic device; and means for detecting a fluorescent signal emitted from the microfluidic device. The working distance between the objective and the device allows light from an external LED or laser to be brought in along a diagonal path to illuminate the microfluidic device. The dry-focus microfluidic device includes multiple channels and multiple closed optical alignment marks having curved walls. At least one of the channels is positioned between at least two of the marks. The marks are illuminated for alignment and focusing purposes by light brought in on a diagonal path from an external white LED.
摘要翻译： 描述了用于微流体装置的光学检测系统和与紧凑型光学检测系统兼容的干焦微流体装置。 该系统包括一个LED; 用于准直LED发出的光的装置; 非球面，熔融二氧化硅物镜; 用于将准直光通过物镜引导到微流体装置上的装置; 以及用于检测从微流体装置发射的荧光信号的装置。 物镜与设备之间的工作距离允许来自外部LED或激光器的光沿着对角线路进入以照亮微流体装置。 干焦微流体装置包括具有弯曲壁的多个通道和多个封闭的光学对准标记。 至少一个通道位于至少两个标记之间。 通过从外部白色LED的对角线路上引入的光照亮标记以进行对准和聚焦。
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公开(公告)号：US20060227325A1
公开(公告)日：2006-10-12
申请号：US11404043
申请日：2006-04-12
申请人： Aaron Rulison , Jeffrey Wolk , Ernest Lee , Michael Slater , Morten Jensen
发明人： Aaron Rulison , Jeffrey Wolk , Ernest Lee , Michael Slater , Morten Jensen
IPC分类号： G01B11/00
CPC分类号： G01B11/002 , B01L3/5027 , B01L3/502715 , B01L2200/025 , B01L2300/0816 , G01N21/645 , G01N2021/0162 , G01N2021/6419 , G01N2021/6421 , G01N2021/6478 , G01N2201/0627 , G02B21/16
摘要： An optical detection system for a microfluidic device and a dry-focus microfluidic device compatible with the compact optical detection system are described. The system includes an LED; means for collimating light emitted by the LED; an aspherical, fused-silica objective lens; means for directing the collimated light through the objective onto a microfluidic device; and means for detecting a fluorescent signal emitted from the microfluidic device. The working distance between the objective and the device allows light from an external LED or laser to be brought in along a diagonal path to illuminate the microfluidic device. The dry-focus microfluidic device includes multiple channels and multiple closed optical alignment marks having curved walls. At least one of the channels is positioned between at least two of the marks. The marks are illuminated for alignment and focusing purposes by light brought in on a diagonal path from an external white LED.
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公开(公告)号：US08993307B2
公开(公告)日：2015-03-31
申请号：US13552497
申请日：2012-07-18
申请人： Denis Zaccarin , Paul Lundquist , Peiqian Zhao , Cheng Frank Zhong , Stephen Turner , Yanqiao Huang , Pezhman Monadgemi , Ravi Saxena , Annette Grot , Aaron Rulison
发明人： Denis Zaccarin , Paul Lundquist , Peiqian Zhao , Cheng Frank Zhong , Stephen Turner , Yanqiao Huang , Pezhman Monadgemi , Ravi Saxena , Annette Grot , Aaron Rulison
CPC分类号： G01N21/6452 , B05D1/005 , C23C16/50 , G01N21/253 , G02B6/12002 , G02B6/131 , G02B6/132 , G02B6/136 , G02B2006/12035 , G02B2006/12061 , G02B2006/12104 , G02B2006/12164 , G02B2006/12197
摘要： Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.
摘要翻译： 用于分析离散反应的设备，系统和方法，以超高的多重复用，降低光学噪声，提高系统灵活性。 装置包括具有集成光学部件的衬底，其通过一个或多个增加密度的反应区域增加多重能力，改善光从离散反应区域发射或收集光。 集成光学部件包括反射光学元件，其重新引导照明光和从离散区域发射的光以更有效地收集发射的光。 特别优选的应用包括单分子反应分析，例如聚合酶介导的模板依赖性核酸合成和序列测定。
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公开(公告)号：US08247216B2
公开(公告)日：2012-08-21
申请号：US12567526
申请日：2009-09-25
申请人： Denis Zaccarin , Paul Lundquist , Peiqian Zhao , Cheng Frank Zhong , Stephen Turner , Yanqiao Huang , Pezhman Monadgemi , Ravi Saxena , Annette Grot , Aaron Rulison
发明人： Denis Zaccarin , Paul Lundquist , Peiqian Zhao , Cheng Frank Zhong , Stephen Turner , Yanqiao Huang , Pezhman Monadgemi , Ravi Saxena , Annette Grot , Aaron Rulison
CPC分类号： G01N21/6452 , B05D1/005 , C23C16/50 , G01N21/253 , G02B6/12002 , G02B6/131 , G02B6/132 , G02B6/136 , G02B2006/12035 , G02B2006/12061 , G02B2006/12104 , G02B2006/12164 , G02B2006/12197
摘要： Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.
摘要翻译： 用于分析离散反应的设备，系统和方法，以超高的多重复用，降低光学噪声，提高系统灵活性。 装置包括具有集成光学部件的衬底，其通过一个或多个增加密度的反应区域增加多重能力，改善光从离散反应区域发射或收集光。 集成光学部件包括反射光学元件，其重新引导照明光和从离散区域发射的光以更有效地收集发射的光。 特别优选的应用包括单分子反应分析，例如聚合酶介导的模板依赖性核酸合成和序列测定。
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公开(公告)号：US20100099100A1
公开(公告)日：2010-04-22
申请号：US12567526
申请日：2009-09-25
申请人： Denis Zaccarin , Paul Lundquist , Peiqian Zhao , Cheng Frank Zhong , Stephen Turner , Yanqiao Huang , Pezhman Monadgemi , Ravi Saxena , Annette Grot , Aaron Rulison
发明人： Denis Zaccarin , Paul Lundquist , Peiqian Zhao , Cheng Frank Zhong , Stephen Turner , Yanqiao Huang , Pezhman Monadgemi , Ravi Saxena , Annette Grot , Aaron Rulison
CPC分类号： G01N21/6452 , B05D1/005 , C23C16/50 , G01N21/253 , G02B6/12002 , G02B6/131 , G02B6/132 , G02B6/136 , G02B2006/12035 , G02B2006/12061 , G02B2006/12104 , G02B2006/12164 , G02B2006/12197
摘要： Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.
摘要翻译： 用于分析离散反应的设备，系统和方法，以超高的多重复用，降低光学噪声，提高系统灵活性。 装置包括具有集成光学部件的衬底，其通过一个或多个增加密度的反应区域增加多重能力，改善光从离散反应区域发射或收集光。 集成光学部件包括反射光学元件，其重新引导照明光和从离散区域发射的光以更有效地收集发射的光。 特别优选的应用包括单分子反应分析，例如聚合酶介导的模板依赖性核酸合成和序列测定。
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公开(公告)号：US20130023039A1
公开(公告)日：2013-01-24
申请号：US13552497
申请日：2012-07-18
申请人： Denis Zaccarin , Paul Lundquist , Peiqian Zhao , Cheng Frank Zhong , Stephen Turner , Yanqiao Huang , Pezhman Monadgemi , Ravi Saxena , Annette Grot , Aaron Rulison
发明人： Denis Zaccarin , Paul Lundquist , Peiqian Zhao , Cheng Frank Zhong , Stephen Turner , Yanqiao Huang , Pezhman Monadgemi , Ravi Saxena , Annette Grot , Aaron Rulison
IPC分类号： C12M1/34
CPC分类号： G01N21/6452 , B05D1/005 , C23C16/50 , G01N21/253 , G02B6/12002 , G02B6/131 , G02B6/132 , G02B6/136 , G02B2006/12035 , G02B2006/12061 , G02B2006/12104 , G02B2006/12164 , G02B2006/12197
摘要： Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.
摘要翻译： 用于分析离散反应的设备，系统和方法，以超高的多重复用，降低光学噪声，提高系统灵活性。 装置包括具有集成光学部件的衬底，其通过一个或多个增加密度的反应区域增加多重能力，改善光从离散反应区域发射或收集光。 集成光学部件包括反射光学元件，其重新引导照明光和从离散区域发射的光以更有效地收集发射的光。 特别优选的应用包括单分子反应分析，例如聚合酶介导的模板依赖性核酸合成和序列测定。
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公开(公告)号：US20050019213A1
公开(公告)日：2005-01-27
申请号：US10861784
申请日：2004-06-04
申请人： Persefoni Kechagia , Michael Greenstein , Bruce Brogden , Ed Donlon , Masayoshi Hayashi , Aaron Rulison
发明人： Persefoni Kechagia , Michael Greenstein , Bruce Brogden , Ed Donlon , Masayoshi Hayashi , Aaron Rulison
CPC分类号： B01L9/527 , B01L3/5027 , B01L3/502715 , B01L99/00 , B01L2300/022 , B01L2300/024 , B01L2400/0415 , B01L2400/0487 , G01N1/40 , G01N27/44704 , G01N27/44773 , G01N27/44791
摘要： This invention provides methods and systems for flushing, washing, and priming microscale devices for reuse. Washing and priming methods include flowing solutions from a manifold to flush wells and microchannels of a microfluidic chip. Systems include manifolds adapted to seal and flow solutions or gasses into chip wells. Devices include microfluidic devices with data storage modules to track the reprocessing status of the microscale devices.
摘要翻译： 本发明提供用于冲洗，洗涤和引发微型装置以重新使用的方法和系统。 洗涤和引发方法包括从歧管流动的溶液冲洗微流体芯片的阱和微通道。 系统包括适于密封并将溶液或气体流入芯片井的歧管。 设备包括具有数据存储模块的微流体设备，以跟踪微型设备的再处理状态。
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