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    Catalyst for preparing polyacrylamide gel which improves the detection of biomaterials by silver staining
    1.
    发明授权
    Catalyst for preparing polyacrylamide gel which improves the detection of biomaterials by silver staining 失效
    用于制备聚丙烯酰胺凝胶的催化剂，其通过银染改善生物材料的检测

    公开(公告)号：US5292665A

    公开(公告)日：1994-03-08

    申请号：US849344

    申请日：1992-03-11

    申请人： Denis F. Hochstrasser ,  Carl R. Merril

    发明人： Denis F. Hochstrasser ,  Carl R. Merril

    IPC分类号： G01N27/447 ,  G01N21/77

    CPC分类号： G01N27/44747 ,  Y10S436/905 ,  Y10T436/25

    摘要： A polyacrylamide gel comprising acrylamide and diacrylylpiperazine. A method for providing a polyacrylamide gel comprising employing a catalyst system which comprises dimethylpiperazine, sodium thiosulfate or a mixture thereof and ammonium or potassium persulfate. The use of the gel as the matrix in a silver staining procedure for the detection of biomaterials, provides for the obtainment of reduced background staining.

    摘要翻译： 包含丙烯酰胺和二丙烯酰基哌嗪的聚丙烯酰胺凝胶。 一种提供聚丙烯酰胺凝胶的方法，包括使用包含二甲基哌嗪，硫代硫酸钠或其混合物和过硫酸铵或过硫酸钾的催化剂体系。 在用于检测生物材料的银染色方法中使用凝胶作为基质，提供了减少的背景染色。

    Pre-cast gel systems for two-dimensional electrophoresis
    2.
    发明授权
    Pre-cast gel systems for two-dimensional electrophoresis 失效
    用于二维电泳的预制凝胶体系

    公开(公告)号：US4874490A

    公开(公告)日：1989-10-17

    申请号：US267069

    申请日：1988-11-04

    申请人： Denis F. Hochstrasser

    发明人： Denis F. Hochstrasser

    IPC分类号： B01D57/02 ,  C07K1/26 ,  G01N27/447

    CPC分类号： G01N27/44773 ,  G01N27/44704

    摘要： A strip gel and a slab gel are combined in a single gel enclosure or on a single gel backing for two-dimensional electrophoresis. The enclosure is comprised of a single pair of support plates, and a removable electrically insulating region is included to separate the gels. The region is occupied either by air or an electrically nonconductive material which can be removed without disturbing either gel. In use, a sample is loaded onto one end of the strip gel and an electric potential is imposed across the strip gel for the first dimension separation while the strip gel is electrically insulated from the slab gel. The insulating region is then removed and the two gels placed in electrical contact for the second dimension separation.

    Stable polyacrylamide gels containing chaotropic agents
    5.
    发明授权
    Stable polyacrylamide gels containing chaotropic agents 失效
    含有离液剂的稳定的聚丙烯酰胺凝胶

    公开(公告)号：US4950708A

    公开(公告)日：1990-08-21

    申请号：US237819

    申请日：1988-08-29

    申请人： Denis F. Hochstrasser

    发明人： Denis F. Hochstrasser

    IPC分类号： C07K1/26 ,  C08F220/56 ,  G01N27/447

    CPC分类号： G01N27/44747 ,  C08F220/56

    摘要： Polyacrylamide gels containing chaotropic agents such as urea as solubilizing agents for protein or nucleic acid separations are rendered stable for prolonged periods of time by the use of certain diacrylyl compounds with tertiary amide groups as cross linking agents. These compounds have the general structure ##STR1## where the R groups are generally saturated hydrocarbon groups and R.sup.4 through R.sup.9 may also be hydrogen. When used as cross linkers, these compounds have the further advantage of preventing urea crystallization at lowered temperatures.