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1.发明授权Geobacter strains that use alternate organic compounds, methods of making, and methods of use thereof 有权使用替代有机化合物的地衣菌株，制备方法及其使用方法公开(公告)号：US09273280B2
公开(公告)日：2016-03-01
申请号：US14070748
申请日：2013-11-04
CPC分类号： C12N1/20 , C12N1/36 , H01M4/90 , H01M8/16 , H01M8/18 , Y02E60/527 , Y02E60/528
摘要： In preferred embodiments, the present invention provides new isolated strains of a Geobacter species that are capable of using a carbon source that is selected from C3 to C12 organic compounds selected from pyruvate or metabolic precursors of pyruvate as an electron donor in metabolism and in subsequent energy production. The wild type strain of the microorganisms has been shown to be unable to use these C3 to C12 organic compounds as electron donors. The inventive strains of microorganisms are useful for improving bioremediation applications, including in situ bioremediation (including uranium bioremediation and halogenated solvent bioremediation), microbial fuel cells, power generation from small and large-scale waste facilities (e.g., biomass waste from dairy, agriculture, food processing, brewery, or vintner industries, etc.) using microbial fuel cells, and other applications of microbial fuel cells, including, but not limited to, improved electrical power supplies for environmental sensors, electronic devices, and electric vehicles.
摘要翻译： 在优选的实施方案中，本发明提供了能够使用选自选自丙酮酸或丙酮酸的代谢前体的C3至C12有机化合物作为代谢和后续能量中的电子给体的碳源的新分离菌株 生产。 微生物的野生型菌株已显示不能使用这些C3-C12有机化合物作为电子给体。 本发明的微生物菌株可用于改善生物修复应用，包括原位生物修复（包括铀生物修复和卤化溶剂生物修复），微生物燃料电池，小型和大型废弃设施的发电（例如，来自乳制品，农业， 使用微生物燃料电池的微生物燃料电池等的应用，包括但不限于用于环境传感器，电子设备和电动车辆的改进的电力供应。
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公开(公告)号：US09074198B2
公开(公告)日：2015-07-07
申请号：US12789294
申请日：2010-05-27
申请人： Derek R. Lovley , Kelly P. Nevin , Hana Yi
发明人： Derek R. Lovley , Kelly P. Nevin , Hana Yi
CPC分类号： C12N1/20 , C12N15/01 , H01M8/16 , Y02E60/527 , Y02P70/56
摘要： Embodiments of the present invention provide a method of producing genetically modified strains of electricigenic microbes that are specifically adapted for the production of electrical current in microbial fuel cells, as well as strains produced by such methods and fuel cells using such strains. In preferred embodiments, the present invention provides genetically modified strains of Geobacter sulfurreducens and methods of using such strains.
摘要翻译： 本发明的实施方案提供了一种生产遗传修饰的致病微生物菌株的方法，其特别适用于在微生物燃料电池中产生电流，以及由这种方法产生的菌株和使用这种菌株的燃料电池。 在优选的实施方案中，本发明提供遗传修饰的地土壤杆菌抗氧化剂菌株和使用这种菌株的方法。
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公开(公告)号：US20080286624A1
公开(公告)日：2008-11-20
申请号：US11750583
申请日：2007-05-18
申请人： Derek R. Lovley , Kelly P. Nevin , Minjuan Zhang , Hongfei Jia
发明人： Derek R. Lovley , Kelly P. Nevin , Minjuan Zhang , Hongfei Jia
CPC分类号： H01M8/16 , H01M4/9008 , H01M4/96 , Y02E60/527
摘要： A microbial fuel cell is provided according to embodiments of the present invention including electricigenic microbes containing at least about 0.075 milligrams of protein per square centimeter of the anode surface area. In particular embodiments, the electricigenic microbes are disposed on the anode such that at least about 90% of the portion of the anode surface area has a layer of electricigenic microbes, the layer greater than about 1 micron in thickness. This thickness is indicative of the layer including at least a first stratum of electricigenic microbes in direct contact with the anode and a second stratum of electricigenic microbes in direct contact with the first stratum such that the second stratum is in indirect contact with the anode.
摘要翻译： 提供根据本发明实施方案的微生物燃料电池，其包括每平方厘米阳极表面积含有至少约0.075毫克蛋白质的发电微生物。 在具体实施方案中，将发电微生物设置在阳极上，使得阳极表面区域的至少约90％的部分具有一层致病微生物，该层厚度大于约1微米。 该厚度指示该层包括与阳极直接接触的至少第一层原生微生物层和与第一层直接接触的第二层原生微生物，使得第二层与阳极间接接触。
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公开(公告)号：US4886752A
公开(公告)日：1989-12-12
申请号：US248220
申请日：1988-09-23
申请人： Derek R. Lovley
发明人： Derek R. Lovley
IPC分类号： C12P3/00
CPC分类号： C12P3/00 , Y10S435/801 , Y10S435/822
摘要： A method of producing magnetite is disclosed which comprises culturing a microorganism designated GS-15 in the presence of organic matter and a ferric iron compound. Unlike prior art production of magnetite using magnetotactic bacteria, GS-15 is able to produce large amounts of ultrafine-grained magnetite extracellularly under anaerobic conditions, allowing for easy separation and recovery of the magnetite without the need for rigorous control over oxygen tensions in the culture medium. As a result, the method of the present invention can be used to mass produce magnetite efficiently using inexpensive means and materials.
摘要翻译： 公开了一种生产磁铁矿的方法，包括在有机物和三价铁化合物的存在下培养指定为GS-15的微生物。 不同于现有技术使用磁能细菌生产磁铁矿，GS-15能够在厌氧条件下在细胞外产生大量的超细晶磁铁矿，从而容易地分离和回收磁铁矿，而不需要严格控制培养物中的氧气张力 中。 结果，本发明的方法可用于使用廉价的装置和材料有效地大量生产磁铁矿。
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5.发明授权Microbial production of multi-carbon chemicals and fuels from water and carbon dioxide using electric current 有权使用电流从水和二氧化碳的微生物生产多碳化学品和燃料公开(公告)号：US09175408B2
公开(公告)日：2015-11-03
申请号：US13514378
申请日：2010-12-22
申请人： Derek R. Lovley , Kelly Nevin
发明人： Derek R. Lovley , Kelly Nevin
CPC分类号： C12M35/02 , C12M21/12 , C12M41/00 , C12P7/16 , C12P7/40 , C12P7/54 , C25B3/00 , C25B3/04 , H01M8/16 , Y02E50/10 , Y02E60/527 , Y02P20/133 , Y02P20/135 , Y02P70/56
摘要： The invention provides systems and methods for generating organic compounds using carbon dioxide as a source of carbon and electrical current as an energy source. In one embodiment, a reaction cell is provided having a cathode electrode and an anode electrode that are connected to a source of electrical power, and which are separated by a permeable membrane. A biological film is provided on the cathode. The biological film comprises a bacterium that can accept electrons and that can convert carbon dioxide to a carbon-bearing compound and water in a cathode half-reaction. At the anode, water is decomposed to free molecular oxygen and solvated protons in an anode half-reaction. The half-reactions are driven by the application of electrical current from an external source. Compounds that have been produced include acetate, butanol, 2-oxobutyrate, propanol, ethanol, and formate.
摘要翻译： 本发明提供了使用二氧化碳作为碳源和电流源作为能量来产生有机化合物的系统和方法。 在一个实施例中，提供具有连接到电源的阴极电极和阳极电极并且由可渗透膜分离的反应池。 在阴极上设置生物膜。 该生物膜包含可以接受电子的细菌并且可以在阴极半反应中将二氧化碳转化为含碳化合物和水。 在阳极处，水在阳极半反应中被分解以释放分子氧和溶剂化质子。 半反应是通过从外部电源施加电流驱动的。 已经生产的化合物包括乙酸酯，丁醇，2-氧代丁酸酯，丙醇，乙醇和甲酸酯。
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6.发明申请Geobacter Strains That Use Alternate Organic Compounds, Methods of Making, and Methods of Use Thereof 有权使用替代有机化合物的地衣芽孢杆菌菌株，制备方法及其使用方法公开(公告)号：US20110151544A1
公开(公告)日：2011-06-23
申请号：US13021953
申请日：2011-02-07
CPC分类号： C12N1/20 , C12N1/36 , H01M4/90 , H01M8/16 , H01M8/18 , Y02E60/527 , Y02E60/528
摘要： In preferred embodiments, the present invention provides new isolated strains of a Geobacter species that are capable of using a carbon source that is selected from C3 to C12 organic compounds selected from pyruvate or metabolic precursors of pyruvate as an electron donor in metabolism and in subsequent energy production. In other aspects, other preferred embodiments of the present invention include methods of making such strains and methods of using such strains. In general, the wild type strain of the microorganisms has been shown to be unable to use these C3 to C12 organic compounds as electron donors in metabolic steps such as the reduction of metallic ions. The inventive strains of microorganisms are useful for improving bioremediation applications, including in situ bioremediation (including uranium bioremediation and halogenated solvent bioremediation), microbial fuel cells, power generation from small and large-scale waste facilities (e.g., biomass waste from dairy, agriculture, food processing, brewery, or vintner industries, etc.) using microbial fuel cells, and other applications of microbial fuel cells, including, but not limited to, improved electrical power supplies for environmental sensors, electronic devices, and electric vehicles.
摘要翻译： 在优选的实施方案中，本发明提供了能够使用选自选自丙酮酸或丙酮酸的代谢前体的C3至C12有机化合物作为代谢和后续能量中的电子给体的碳源的新分离菌株 生产。 在其他方面，本发明的其它优选实施方案包括制备这种菌株的方法和使用这些菌株的方法。 通常，野生型菌株已被证明在代谢步骤如金属离子的还原中不能使用这些C3-C12有机化合物作为电子给体。 本发明的微生物菌株可用于改善生物修复应用，包括原位生物修复（包括铀生物修复和卤化溶剂生物修复），微生物燃料电池，小型和大型废弃设施的发电（例如，乳品，农业， 使用微生物燃料电池的微生物燃料电池等的应用，包括但不限于用于环境传感器，电子设备和电动车辆的改进的电力供应。
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公开(公告)号：US20100304189A1
公开(公告)日：2010-12-02
申请号：US12789294
申请日：2010-05-27
申请人： Derek R. Lovley , Kelly P. Nevin , Hana Yi
发明人： Derek R. Lovley , Kelly P. Nevin , Hana Yi
CPC分类号： C12N1/20 , C12N15/01 , H01M8/16 , Y02E60/527 , Y02P70/56
摘要： Embodiments of the present invention provide a method of producing genetically modified strains of electricigenic microbes that are specifically adapted for the production of electrical current in microbial fuel cells, as well as strains produced by such methods and fuel cells using such strains. In preferred embodiments, the present invention provides genetically modified strains of Geobacter sulfurreducens and methods of using such strains.
摘要翻译： 本发明的实施方案提供了一种生产遗传修饰的致病微生物菌株的方法，其特别适用于在微生物燃料电池中产生电流，以及由这种方法产生的菌株和使用这种菌株的燃料电池。 在优选的实施方案中，本发明提供遗传修饰的地土壤杆菌抗氧化剂菌株和使用这种菌株的方法。
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8.发明申请SYSTEMS AND METHODS FOR MICROBIAL REDUCTIVE DECHLORINATION OF ENVIRONMENTAL CONTAMINANTS 有权微生物减少环境污染物去除的系统和方法公开(公告)号：US20100059436A1
公开(公告)日：2010-03-11
申请号：US12538744
申请日：2009-08-10
申请人： Derek R. Lovley , Sarah Strycharz , Frank Loeffler
发明人： Derek R. Lovley , Sarah Strycharz , Frank Loeffler
摘要： In preferred embodiments, bioremediation systems are provided that comprise electricigenic microbes that use electrons provided directly from the anode of an electrical bioremediation system to carry out reductive dehalogenation of halogenated hydrocarbon contaminants, including chlorinated solvents. The present invention also provides methods of performing in situ bioremediation of halogenated solvents in groundwater or soil through the use of the provided systems.
摘要翻译： 在优选的实施方案中，提供了生物修复系统，其包括使用直接从电生物修复系统的阳极提供的电子进行卤代烃污染物（包括氯化溶剂）的还原性脱卤的电致生物微生物。 本发明还提供了通过使用所提供的系统对地下水或土壤中的卤化溶剂进行原位生物修复的方法。
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9.发明授权Geobacter strains that use alternate organic compounds, methods of making, and methods of use thereof 有权使用替代有机化合物的地衣菌株，制备方法及其使用方法公开(公告)号：US08597930B2
公开(公告)日：2013-12-03
申请号：US13021953
申请日：2011-02-07
IPC分类号： C12N1/20
CPC分类号： C12N1/20 , C12N1/36 , H01M4/90 , H01M8/16 , H01M8/18 , Y02E60/527 , Y02E60/528
摘要： In preferred embodiments, the present invention provides new isolated strains of a Geobacter species that are capable of using a carbon source that is selected from C3 to C12 organic compounds selected from pyruvate or metabolic precursors of pyruvate as an electron donor in metabolism and in subsequent energy production. In other aspects, other preferred embodiments of the present invention include methods of making such strains and methods of using such strains. In general, the wild type strain of the microorganisms has been shown to be unable to use these C3 to C12 organic compounds as electron donors in metabolic steps such as the reduction of metallic ions. The inventive strains of microorganisms are useful for improving bioremediation applications, including in situ bioremediation (including uranium bioremediation and halogenated solvent bioremediation), microbial fuel cells, power generation from small and large-scale waste facilities (e.g., biomass waste from dairy, agriculture, food processing, brewery, or vintner industries, etc.) using microbial fuel cells, and other applications of microbial fuel cells, including, but not limited to, improved electrical power supplies for environmental sensors, electronic devices, and electric vehicles.
摘要翻译： 在优选的实施方案中，本发明提供了能够使用选自选自丙酮酸或丙酮酸的代谢前体的C3至C12有机化合物作为代谢和后续能量中的电子给体的碳源的新分离菌株 生产。 在其他方面，本发明的其它优选实施方案包括制备这种菌株的方法和使用这些菌株的方法。 通常，野生型菌株已被证明在代谢步骤如金属离子的还原中不能使用这些C3-C12有机化合物作为电子给体。 本发明的微生物菌株可用于改善生物修复应用，包括原位生物修复（包括铀生物修复和卤化溶剂生物修复），微生物燃料电池，小型和大型废弃设施的发电（例如，来自乳制品，农业， 使用微生物燃料电池的微生物燃料电池等的应用，包括但不限于用于环境传感器，电子设备和电动车辆的改进的电力供应。
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10.发明授权Systems and methods for microbial reductive dechlorination of environmental contaminants 有权环境污染物微生物还原脱氯的系统和方法公开(公告)号：US08277657B2
公开(公告)日：2012-10-02
申请号：US12538744
申请日：2009-08-10
申请人： Derek R. Lovley , Sarah Strycharz , Frank Loeffler
发明人： Derek R. Lovley , Sarah Strycharz , Frank Loeffler
IPC分类号： C02F3/00
摘要： In preferred embodiments, bioremediation systems are provided that comprise electricigenic microbes that use electrons provided directly from the anode of an electrical bioremediation system to carry out reductive dehalogenation of halogenated hydrocarbon contaminants, including chlorinated solvents. The present invention also provides methods of performing in situ bioremediation of halogenated solvents in groundwater or soil through the use of the provided systems.
摘要翻译： 在优选的实施方案中，提供了生物修复系统，其包括使用直接从电生物修复系统的阳极提供的电子进行卤代烃污染物（包括氯化溶剂）的还原性脱卤的电致生物微生物。 本发明还提供了通过使用所提供的系统对地下水或土壤中的卤化溶剂进行原位生物修复的方法。
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